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The map label for this gene is mltB [H]

Identifier: 158423847

GI number: 158423847

Start: 2544151

End: 2545416

Strand: Direct

Name: mltB [H]

Synonym: AZC_2223

Alternate gene names: 158423847

Gene position: 2544151-2545416 (Clockwise)

Preceding gene: 158423846

Following gene: 158423848

Centisome position: 47.38

GC content: 68.48

Gene sequence:

>1266_bases
ATGGACGACCACATGCGGCCCGCGCGGCACGGGATCGATCGCCGCCTGTTCCTAGGTGCACTGACTGCCGGCACGGCCGG
TTTCCTCACACCCGGTCTGGCGCTTGCCCAGAGCGCGGCGCAGCCGTTCGGCACCTGGGTGGAGAAGTTTCGCGCGCGGG
CCCGTGCCCGCGGCATTTCGGACGCCACCTATTCCCGCGTCTTCGCGACCGTGAAGCCCGACCCGAGCGTGTTCGAGGCG
GATCGGGCGCAGCCCGAGTTTCAGGAAGAGACGTGGCAATATCTCAACCGCCGCGTTTCGGACTGGCGCATCGCCACCGG
CCGCCAGCGGGCGTCGGAATATGCCGCCCTGTTCACCCGGATCGAGAATGATTTCGGCGTCGATCGCGCGGTCATGCTCG
GGTACTGGGGCATGGAATCGGCGTTCGGCGACGTGGTGACCAATCCCCGTCACATGAAGCCGGTCTTCAACTCGCTCGCG
ACTCTCGCCTGGGGCGATCCGCGCCGGCGCAAATATTGGGAGACCGAGCTTCTCAACGCGCTCGTCATCGTGGAGCGCGG
GTGGAGCACGCCGCAGGAGATGGTCGGCTCCTGGGCGGGAGCCATGGGGCACACCCAATGGATGCCCGAGGTCTGGCTGA
ACATGGGCGTCGACTATGACGGCAACGGCCGTATCTCGCCCTTCGGCAAGCCGGACGACGCGCTGGCCGGCACCGCCAAT
TTCATCCTGAAGCGCGGCCGCTACCGCAAAGGCGAGCCCTGGGGGATCGAGGCCCGACTTCCGGCCGGATTCAACACGCA
GCTTGCGGACAACAAGACCTGGCGCCCGCTTTCCGCCTGGGGCGGCATGGGGCTGACCATGGTCGAGGGGCAGTCCATCA
CCGAATATGACAACCCCGCCCGCCTGTGGCTGCCCGGCGGCGCCGGCGGACCGGCGCTGCTGCTGCTCCACAATTTCTAT
GCGGTGCGCTCCTATAATCCCTCCTCCGCTTATGCGCTGGCGGTGGTGCATCTCGGCGACCGGGTGATGGGCGGCGAGCC
GTTCAGGACGCCCTGGCCGGGCGGCGAGCGCACCTTGACGCTGGCCGAATTGCAGGAGATCCAGCAGCGCCTCACCCGCC
TCGGCTTCGACACCGGCGGGACCGACGGGCGGGTGGGCAATGACACCATGCGCGCGGTCAAGGCCTTCCAGCAGCGCGCC
GGCCTCACCCCGGCGGACGGCTATCCCGGCCTCCAGGTGCTGACGGCGCTGCGCGGAGCCCGCTGA

Upstream 100 bases:

>100_bases
TCGCGTCCTGAGGCCGCGCCGATCACAAGGGCGCGGGCGCGCTGACGTTGCGGCAATTCGGGCTCGCCCGTGCCATCAGA
ATCTGGGAGGCTCCCGCGCC

Downstream 100 bases:

>100_bases
AATACGCGCCACCGCTGGATTTGCGCTCATCCTGAATTGACAGGCTGACGCATCGGGGGTATGAGCGCCCACTCTTCGTG
GGTCGGTTCGCCGGCCCGCG

Product: lytic murein transglycosylase

Products: Muramic Acid Residue [C]

Alternate protein names: 35 kDa soluble lytic transglycosylase; Murein hydrolase B; Slt35 [H]

Number of amino acids: Translated: 421; Mature: 421

Protein sequence:

>421_residues
MDDHMRPARHGIDRRLFLGALTAGTAGFLTPGLALAQSAAQPFGTWVEKFRARARARGISDATYSRVFATVKPDPSVFEA
DRAQPEFQEETWQYLNRRVSDWRIATGRQRASEYAALFTRIENDFGVDRAVMLGYWGMESAFGDVVTNPRHMKPVFNSLA
TLAWGDPRRRKYWETELLNALVIVERGWSTPQEMVGSWAGAMGHTQWMPEVWLNMGVDYDGNGRISPFGKPDDALAGTAN
FILKRGRYRKGEPWGIEARLPAGFNTQLADNKTWRPLSAWGGMGLTMVEGQSITEYDNPARLWLPGGAGGPALLLLHNFY
AVRSYNPSSAYALAVVHLGDRVMGGEPFRTPWPGGERTLTLAELQEIQQRLTRLGFDTGGTDGRVGNDTMRAVKAFQQRA
GLTPADGYPGLQVLTALRGAR

Sequences:

>Translated_421_residues
MDDHMRPARHGIDRRLFLGALTAGTAGFLTPGLALAQSAAQPFGTWVEKFRARARARGISDATYSRVFATVKPDPSVFEA
DRAQPEFQEETWQYLNRRVSDWRIATGRQRASEYAALFTRIENDFGVDRAVMLGYWGMESAFGDVVTNPRHMKPVFNSLA
TLAWGDPRRRKYWETELLNALVIVERGWSTPQEMVGSWAGAMGHTQWMPEVWLNMGVDYDGNGRISPFGKPDDALAGTAN
FILKRGRYRKGEPWGIEARLPAGFNTQLADNKTWRPLSAWGGMGLTMVEGQSITEYDNPARLWLPGGAGGPALLLLHNFY
AVRSYNPSSAYALAVVHLGDRVMGGEPFRTPWPGGERTLTLAELQEIQQRLTRLGFDTGGTDGRVGNDTMRAVKAFQQRA
GLTPADGYPGLQVLTALRGAR
>Mature_421_residues
MDDHMRPARHGIDRRLFLGALTAGTAGFLTPGLALAQSAAQPFGTWVEKFRARARARGISDATYSRVFATVKPDPSVFEA
DRAQPEFQEETWQYLNRRVSDWRIATGRQRASEYAALFTRIENDFGVDRAVMLGYWGMESAFGDVVTNPRHMKPVFNSLA
TLAWGDPRRRKYWETELLNALVIVERGWSTPQEMVGSWAGAMGHTQWMPEVWLNMGVDYDGNGRISPFGKPDDALAGTAN
FILKRGRYRKGEPWGIEARLPAGFNTQLADNKTWRPLSAWGGMGLTMVEGQSITEYDNPARLWLPGGAGGPALLLLHNFY
AVRSYNPSSAYALAVVHLGDRVMGGEPFRTPWPGGERTLTLAELQEIQQRLTRLGFDTGGTDGRVGNDTMRAVKAFQQRA
GLTPADGYPGLQVLTALRGAR

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG2951

COG function: function code M; Membrane-bound lytic murein transglycosylase B

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor; Periplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789053, Length=255, Percent_Identity=29.8039215686275, Blast_Score=97, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011757 [H]

Pfam domain/function: NA

EC number: 3.2.1.- [C]

Molecular weight: Translated: 46682; Mature: 46682

Theoretical pI: Translated: 9.82; Mature: 9.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDDHMRPARHGIDRRLFLGALTAGTAGFLTPGLALAQSAAQPFGTWVEKFRARARARGIS
CCCCCCHHHHCCCHHHHHHHHHCCCHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCC
DATYSRVFATVKPDPSVFEADRAQPEFQEETWQYLNRRVSDWRIATGRQRASEYAALFTR
HHHHHHEEEEECCCCCHHCCCCCCCCHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHH
IENDFGVDRAVMLGYWGMESAFGDVVTNPRHMKPVFNSLATLAWGDPRRRKYWETELLNA
HHHHCCCCHHHHEECCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHE
LVIVERGWSTPQEMVGSWAGAMGHTQWMPEVWLNMGVDYDGNGRISPFGKPDDALAGTAN
EEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHH
FILKRGRYRKGEPWGIEARLPAGFNTQLADNKTWRPLSAWGGMGLTMVEGQSITEYDNPA
HHHHCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCHHHHCCCCEEEECCCCCCCCCCCC
RLWLPGGAGGPALLLLHNFYAVRSYNPSSAYALAVVHLGDRVMGGEPFRTPWPGGERTLT
EEEECCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEEECCCHHCCCCCCCCCCCCCCCEEE
LAELQEIQQRLTRLGFDTGGTDGRVGNDTMRAVKAFQQRAGLTPADGYPGLQVLTALRGA
HHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCC
R
C
>Mature Secondary Structure
MDDHMRPARHGIDRRLFLGALTAGTAGFLTPGLALAQSAAQPFGTWVEKFRARARARGIS
CCCCCCHHHHCCCHHHHHHHHHCCCHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCC
DATYSRVFATVKPDPSVFEADRAQPEFQEETWQYLNRRVSDWRIATGRQRASEYAALFTR
HHHHHHEEEEECCCCCHHCCCCCCCCHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHH
IENDFGVDRAVMLGYWGMESAFGDVVTNPRHMKPVFNSLATLAWGDPRRRKYWETELLNA
HHHHCCCCHHHHEECCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHE
LVIVERGWSTPQEMVGSWAGAMGHTQWMPEVWLNMGVDYDGNGRISPFGKPDDALAGTAN
EEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHH
FILKRGRYRKGEPWGIEARLPAGFNTQLADNKTWRPLSAWGGMGLTMVEGQSITEYDNPA
HHHHCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCHHHHCCCCEEEECCCCCCCCCCCC
RLWLPGGAGGPALLLLHNFYAVRSYNPSSAYALAVVHLGDRVMGGEPFRTPWPGGERTLT
EEEECCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEEECCCHHCCCCCCCCCCCCCCCEEE
LAELQEIQQRLTRLGFDTGGTDGRVGNDTMRAVKAFQQRAGLTPADGYPGLQVLTALRGA
HHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCC
R
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

General reaction: Cleavage Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7476170; 7789526; 9205837; 9278503; 3553176; 9761817; 10570954; 10545329; 10684641 [H]