Click here to switch to the map view.

The map label for this gene is prpB [H]

Identifier: 158423739

GI number: 158423739

Start: 2431974

End: 2432879

Strand: Direct

Name: prpB [H]

Synonym: AZC_2115

Alternate gene names: 158423739

Gene position: 2431974-2432879 (Clockwise)

Preceding gene: 158423738

Following gene: 158423740

Centisome position: 45.29

GC content: 67.33

Gene sequence:

>906_bases
ATGAGTCTGAAAGCCCGCCTTGCGCAGGAGCGCGTCCTGCCCGCGCCGGGCATCTATGATGCCCTCACGGCTTCGCTCGC
GGCCGCCGCCGGCTTCGAGGCCCTGTATCTCTCCGGCGCCGCCATTGCCTATACGCGCCTCGGGCGGCCGGACATCGGCC
TCGTCTCCATGACCGAGGTGGCCGAGGTGATCGCACTGGTTCGCGACCGCGTGCCGACGCCAGTGATTGTCGATGCGGAC
AATGGCTATGGCAACGCGCTGAACGTGCAGCGCACGGTGCGGACCTTCGAGCGGGCGGGCGCAAGCGCCCTCCAGCTTGA
GGATCAGACCATGCCCAAGCGCTGCGGCCATTTGCAGGACAAGAGCCTGATCAGCACCGCCGAGATGATCGGTAAGATCA
AGGCCGCAGTGGACGCGCGGACCAGTGAGGAGACGCTCATCATCGCCCGAACCGATGCGGTGGCGGTGGAGGGCTTCGAG
CCGGCCATCGAGCGCGCCCGGCTCTATGCGGAAGCCGGCGCGGACGTACTGTTCGTGGAGGCGCCGCGCTCGCAGGACCA
GCTTGCCGCCGTCACGCGGGCGCTGGGCAATGGCTTGCCCTTGCTCGTGAACATGGTGGAAGGCGGCGACACGCCGCTCG
CGACCACCGAGGAACTGGGCGAGCTCGGCTTCAAGATCGTGATCTTCCCGGGCGGAATCGTGCGCGCGCTCGCCCGCACG
GCGCAAGCCTATTACCGCTCGCTCTTCACCAATGGCAGCAACGAGCCGTTCCGCGAGCACATGTTCGATTTCTCCGCCCT
CAACGGCCTCATCGGCACCGCCGACCTGCTGGCCCAAGGGCGTGAGTATGATGTCTTCGGCGCGGCTCTGGCCGATGCGC
CGCTCACCAGGAAAGCCGCGCGATGA

Upstream 100 bases:

>100_bases
GATCTTCTGGCCTGAGGCGAAACCGCCTCCGGCTTCTGTTGTGAACGGCGCCGTCCGGCCCATCGCTCCGGACCCCGCCC
CTTTGGGAAAGACCTGTGCC

Downstream 100 bases:

>100_bases
CCGCCATCGACCCCGTCACACTCGCCGTCCTGAAGGGGCGGCTGGAGCAGATCGCCGACGAGATGGATGCGACGCTCTAC
CGCTCCGCTTTCAATCCCAT

Product: isocitrate lyase family protein

Products: NA

Alternate protein names: 2-methylisocitrate lyase [H]

Number of amino acids: Translated: 301; Mature: 300

Protein sequence:

>301_residues
MSLKARLAQERVLPAPGIYDALTASLAAAAGFEALYLSGAAIAYTRLGRPDIGLVSMTEVAEVIALVRDRVPTPVIVDAD
NGYGNALNVQRTVRTFERAGASALQLEDQTMPKRCGHLQDKSLISTAEMIGKIKAAVDARTSEETLIIARTDAVAVEGFE
PAIERARLYAEAGADVLFVEAPRSQDQLAAVTRALGNGLPLLVNMVEGGDTPLATTEELGELGFKIVIFPGGIVRALART
AQAYYRSLFTNGSNEPFREHMFDFSALNGLIGTADLLAQGREYDVFGAALADAPLTRKAAR

Sequences:

>Translated_301_residues
MSLKARLAQERVLPAPGIYDALTASLAAAAGFEALYLSGAAIAYTRLGRPDIGLVSMTEVAEVIALVRDRVPTPVIVDAD
NGYGNALNVQRTVRTFERAGASALQLEDQTMPKRCGHLQDKSLISTAEMIGKIKAAVDARTSEETLIIARTDAVAVEGFE
PAIERARLYAEAGADVLFVEAPRSQDQLAAVTRALGNGLPLLVNMVEGGDTPLATTEELGELGFKIVIFPGGIVRALART
AQAYYRSLFTNGSNEPFREHMFDFSALNGLIGTADLLAQGREYDVFGAALADAPLTRKAAR
>Mature_300_residues
SLKARLAQERVLPAPGIYDALTASLAAAAGFEALYLSGAAIAYTRLGRPDIGLVSMTEVAEVIALVRDRVPTPVIVDADN
GYGNALNVQRTVRTFERAGASALQLEDQTMPKRCGHLQDKSLISTAEMIGKIKAAVDARTSEETLIIARTDAVAVEGFEP
AIERARLYAEAGADVLFVEAPRSQDQLAAVTRALGNGLPLLVNMVEGGDTPLATTEELGELGFKIVIFPGGIVRALARTA
QAYYRSLFTNGSNEPFREHMFDFSALNGLIGTADLLAQGREYDVFGAALADAPLTRKAAR

Specific function: Catalyzes the formation of pyruvate and succinate from 2-methylisocitrate [H]

COG id: COG2513

COG function: function code G; PEP phosphonomutase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate lyase/PEP mutase superfamily. Methylisocitrate lyase family [H]

Homologues:

Organism=Escherichia coli, GI1786525, Length=263, Percent_Identity=41.06463878327, Blast_Score=186, Evalue=1e-48,
Organism=Escherichia coli, GI1790445, Length=159, Percent_Identity=32.7044025157233, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000918
- InterPro:   IPR018523
- InterPro:   IPR012695
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00463 ICL [H]

EC number: =4.1.3.30 [H]

Molecular weight: Translated: 32051; Mature: 31920

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS00161 ISOCITRATE_LYASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLKARLAQERVLPAPGIYDALTASLAAAAGFEALYLSGAAIAYTRLGRPDIGLVSMTEV
CCCHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHHHCCCHHEEEECCCCCCCCEEHHHH
AEVIALVRDRVPTPVIVDADNGYGNALNVQRTVRTFERAGASALQLEDQTMPKRCGHLQD
HHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEECHHHHHHHHCCCCH
KSLISTAEMIGKIKAAVDARTSEETLIIARTDAVAVEGFEPAIERARLYAEAGADVLFVE
HHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCEEEECCCHHHHHHHHHHHHCCCCEEEEE
APRSQDQLAAVTRALGNGLPLLVNMVEGGDTPLATTEELGELGFKIVIFPGGIVRALART
CCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHHHHCCCEEEEECCHHHHHHHHH
AQAYYRSLFTNGSNEPFREHMFDFSALNGLIGTADLLAQGREYDVFGAALADAPLTRKAA
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCHHHCC
R
C
>Mature Secondary Structure 
SLKARLAQERVLPAPGIYDALTASLAAAAGFEALYLSGAAIAYTRLGRPDIGLVSMTEV
CCHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHHHCCCHHEEEECCCCCCCCEEHHHH
AEVIALVRDRVPTPVIVDADNGYGNALNVQRTVRTFERAGASALQLEDQTMPKRCGHLQD
HHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEECHHHHHHHHCCCCH
KSLISTAEMIGKIKAAVDARTSEETLIIARTDAVAVEGFEPAIERARLYAEAGADVLFVE
HHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCEEEECCCHHHHHHHHHHHHCCCCEEEEE
APRSQDQLAAVTRALGNGLPLLVNMVEGGDTPLATTEELGELGFKIVIFPGGIVRALART
CCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHHHHCCCEEEEECCHHHHHHHHH
AQAYYRSLFTNGSNEPFREHMFDFSALNGLIGTADLLAQGREYDVFGAALADAPLTRKAA
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCHHHCC
R
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10382966 [H]