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The map label for this gene is braE [H]

Identifier: 158423735

GI number: 158423735

Start: 2428081

End: 2429052

Strand: Direct

Name: braE [H]

Synonym: AZC_2111

Alternate gene names: 158423735

Gene position: 2428081-2429052 (Clockwise)

Preceding gene: 158423734

Following gene: 158423736

Centisome position: 45.22

GC content: 67.28

Gene sequence:

>972_bases
ATGACGCGCGGCATCCTCCCCCTTCTCGCCCTTGTGGTACTCATCGCGGCGCTGCCGCTGGTGACGGCCTCCAACACGGC
CATCAATTTCGTCGTCTTCGTGCTCATCATCGCTCTGGGCGCGCAGGGCTGGAACCTGCTCGGTGGTGTCGGCGGCCAGT
TCTCCTTCGGCCATGCGGCCTTCTTCGGCACGGGCGCCTATGTCTCGGCCGTGCTTCAGGCCCGCTTCGGCGTGAACGCC
TGGGTGGCCTTCGCGGCCGGTGTCGCCATGGGCGCGCTGGTGGGCTGGGTCATTGGTTTCCTGAGCTTCCGGGCCGGCCT
GCGTGGCTCCTATTTCGCTCTTGTCACGCTCGCATTCGCGGAAGTGTTGCGCATTCTTGCCAATGCCCTGCCCATCACCG
GCGGCGGCGCGGGCCTGCTGCTGAAGCTCGATTTGGGCCTCGGCAACTTCCAGTTCTCCAGCCGCGCCGTTTTCCTCTGG
ATCGCGCTGGCGCTGGTGGCGGCGGGGCTTCTGCTGTGCCGCTGGATCGCGGATTCTCGCTTCGGCGCGCAGCTCGTCGC
CGTGCGCGAGAATGAGGATGCGGCGCGCGCGCTCGGCGTCGACGTGCTGAAGGTGAAGCTCAAGGCCATTACCCTTTCCG
GCGCGCTCACCGCCGCTGCCGGGGCCTTCTATCTTCAGTATTTCCTCTATCTCGACGCCAACATCGCCTATGGCGTGTGG
ATCTCGGTGGAGGTGCTGCTGGCGGCCATGGTGGGCGGCCGGGGCACGGTGCTCGGGCCGCTGGTGGGCGCTTTCGCGCT
CCATGGCCTCGGCGATGTCACCAAGCAATATGCCGGCGGCATTCCCGGCATCGATCTCGCCGTCTATGGCGCGGTGCTGA
TCTTCGTGGTGGCCTTCGCCCCCTCGGGCCTCATCGGGCTGGTGCAACGTCTGTTCGCCCGCCGCTCCACGGCCGCGCAG
GGAGGCGCATGA

Upstream 100 bases:

>100_bases
GCCTCTTCCTCGGCGACAGCCTGGGCCAGATCGGCATCTTCCTCATCTTCATCCTTGTCCTGCTGCTGCGGCCCAGCGGC
CTGTTCGGAGCCCGGGCCTG

Downstream 100 bases:

>100_bases
TGCTGGAGGTCTCCGACATCACCATGCGCTTCGGTGGCCTCGTCGCCATCTCGGACGCCTCCTTGAGCGTGCCGAAGGGC
GGGCTCGTGGGGCTCATCGG

Product: amino acid ABC transporter permease

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 323; Mature: 322

Protein sequence:

>323_residues
MTRGILPLLALVVLIAALPLVTASNTAINFVVFVLIIALGAQGWNLLGGVGGQFSFGHAAFFGTGAYVSAVLQARFGVNA
WVAFAAGVAMGALVGWVIGFLSFRAGLRGSYFALVTLAFAEVLRILANALPITGGGAGLLLKLDLGLGNFQFSSRAVFLW
IALALVAAGLLLCRWIADSRFGAQLVAVRENEDAARALGVDVLKVKLKAITLSGALTAAAGAFYLQYFLYLDANIAYGVW
ISVEVLLAAMVGGRGTVLGPLVGAFALHGLGDVTKQYAGGIPGIDLAVYGAVLIFVVAFAPSGLIGLVQRLFARRSTAAQ
GGA

Sequences:

>Translated_323_residues
MTRGILPLLALVVLIAALPLVTASNTAINFVVFVLIIALGAQGWNLLGGVGGQFSFGHAAFFGTGAYVSAVLQARFGVNA
WVAFAAGVAMGALVGWVIGFLSFRAGLRGSYFALVTLAFAEVLRILANALPITGGGAGLLLKLDLGLGNFQFSSRAVFLW
IALALVAAGLLLCRWIADSRFGAQLVAVRENEDAARALGVDVLKVKLKAITLSGALTAAAGAFYLQYFLYLDANIAYGVW
ISVEVLLAAMVGGRGTVLGPLVGAFALHGLGDVTKQYAGGIPGIDLAVYGAVLIFVVAFAPSGLIGLVQRLFARRSTAAQ
GGA
>Mature_322_residues
TRGILPLLALVVLIAALPLVTASNTAINFVVFVLIIALGAQGWNLLGGVGGQFSFGHAAFFGTGAYVSAVLQARFGVNAW
VAFAAGVAMGALVGWVIGFLSFRAGLRGSYFALVTLAFAEVLRILANALPITGGGAGLLLKLDLGLGNFQFSSRAVFLWI
ALALVAAGLLLCRWIADSRFGAQLVAVRENEDAARALGVDVLKVKLKAITLSGALTAAAGAFYLQYFLYLDANIAYGVWI
SVEVLLAAMVGGRGTVLGPLVGAFALHGLGDVTKQYAGGIPGIDLAVYGAVLIFVVAFAPSGLIGLVQRLFARRSTAAQG
GA

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=322, Percent_Identity=26.3975155279503, Blast_Score=75, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 33245; Mature: 33114

Theoretical pI: Translated: 10.28; Mature: 10.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRGILPLLALVVLIAALPLVTASNTAINFVVFVLIIALGAQGWNLLGGVGGQFSFGHAA
CCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCHH
FFGTGAYVSAVLQARFGVNAWVAFAAGVAMGALVGWVIGFLSFRAGLRGSYFALVTLAFA
HHCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
EVLRILANALPITGGGAGLLLKLDLGLGNFQFSSRAVFLWIALALVAAGLLLCRWIADSR
HHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
FGAQLVAVRENEDAARALGVDVLKVKLKAITLSGALTAAAGAFYLQYFLYLDANIAYGVW
CCCEEEEEECCCHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHHCCCCHHHHH
ISVEVLLAAMVGGRGTVLGPLVGAFALHGLGDVTKQYAGGIPGIDLAVYGAVLIFVVAFA
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHC
PSGLIGLVQRLFARRSTAAQGGA
CCCHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TRGILPLLALVVLIAALPLVTASNTAINFVVFVLIIALGAQGWNLLGGVGGQFSFGHAA
CCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCHH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CCCHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]