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The map label for this gene is puuR [H]

Identifier: 158423697

GI number: 158423697

Start: 2385931

End: 2386626

Strand: Direct

Name: puuR [H]

Synonym: AZC_2073

Alternate gene names: 158423697

Gene position: 2385931-2386626 (Clockwise)

Preceding gene: 158423691

Following gene: 158423710

Centisome position: 44.43

GC content: 64.94

Gene sequence:

>696_bases
TTGGAACTGTGCGACGTCTTTCGTAATTCGATGAACTCTGTGATGGGCCATCGGAAATCGTTTCATTCAGGGATCGTTTT
CACGATGGTGAAGGGGCGGGCCGAAGTGGCGTCAACCGAGGACGAGGCGGCGAGCGAGCATGATCGTGCCTTCGGGTCCA
GCATCCGCGAGGCGCGGCTCGCGCGCGGGCTTTCGCTCCAGCGCGTGGCGGATGCGGCCGGTCTTTCGGTGGGGTTGCTC
AGCCAGGTGGAGCGCGGGATTTCCTCGCCCTCCGTGCGCGTGCTGCGGGCGATCTGCGGTGCCCTTGGAGTCACCGTGCA
ATCGCTGTTCGGGGAGGACGGGGCTCCGCTCTCCGAGAGCCGCCATGTGATGCGGCCTTACCAACGCCGGCGGCTCGATC
TCGGAGCCAAGGGCATCGTGAAGGAATTCCTCAGCCGCAGCGATCTCAGCACGCTGCAGATCATGGAAATCATCCTCGCG
CCCGGCGGCAGTTCCGGCGAGGAGGCCTATAACCACATTGGCGAGGAAGGCGGCGTGGTGGTGGAAGGCGCGATGGAACT
CGTCATCGACGGCACCGTGCACCGCCTCATGCAGGGGGATTCCTTCGCCTTCGAGAGCGCCCTGCCGCACAAGTTCCGCA
ACCTCTCGGAGGGCGTCACCCGCGTGCTCTGGATCACCACGCCGCCGGTGTGGTGA

Upstream 100 bases:

>100_bases
GCTGCCTTGGCCGGTCCGCATCGGAACCGCCGCAGCCACTATAAGCCAACCCTGCACCTCGGCAATGGGCGCAGGTTGCA
AAATTTCACTTTTATGAAAA

Downstream 100 bases:

>100_bases
GGCCGGCGGCGCCCGCTCAGGACAGTCGGGCCATCAGGCCGGCGATGAGGCGGCCCCGCTCGGCCAAGGTGTCGATGCGG
ATGTGCTCGTTGAGCGTGTG

Product: XRE family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 231; Mature: 231

Protein sequence:

>231_residues
MELCDVFRNSMNSVMGHRKSFHSGIVFTMVKGRAEVASTEDEAASEHDRAFGSSIREARLARGLSLQRVADAAGLSVGLL
SQVERGISSPSVRVLRAICGALGVTVQSLFGEDGAPLSESRHVMRPYQRRRLDLGAKGIVKEFLSRSDLSTLQIMEIILA
PGGSSGEEAYNHIGEEGGVVVEGAMELVIDGTVHRLMQGDSFAFESALPHKFRNLSEGVTRVLWITTPPVW

Sequences:

>Translated_231_residues
MELCDVFRNSMNSVMGHRKSFHSGIVFTMVKGRAEVASTEDEAASEHDRAFGSSIREARLARGLSLQRVADAAGLSVGLL
SQVERGISSPSVRVLRAICGALGVTVQSLFGEDGAPLSESRHVMRPYQRRRLDLGAKGIVKEFLSRSDLSTLQIMEIILA
PGGSSGEEAYNHIGEEGGVVVEGAMELVIDGTVHRLMQGDSFAFESALPHKFRNLSEGVTRVLWITTPPVW
>Mature_231_residues
MELCDVFRNSMNSVMGHRKSFHSGIVFTMVKGRAEVASTEDEAASEHDRAFGSSIREARLARGLSLQRVADAAGLSVGLL
SQVERGISSPSVRVLRAICGALGVTVQSLFGEDGAPLSESRHVMRPYQRRRLDLGAKGIVKEFLSRSDLSTLQIMEIILA
PGGSSGEEAYNHIGEEGGVVVEGAMELVIDGTVHRLMQGDSFAFESALPHKFRNLSEGVTRVLWITTPPVW

Specific function: Represses puuD [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH cro/C1-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787557, Length=188, Percent_Identity=29.7872340425532, Blast_Score=91, Evalue=6e-20,
Organism=Escherichia coli, GI1787704, Length=166, Percent_Identity=27.1084337349398, Blast_Score=65, Evalue=4e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013096
- InterPro:   IPR011051
- InterPro:   IPR001387
- InterPro:   IPR010982
- InterPro:   IPR014710 [H]

Pfam domain/function: PF07883 Cupin_2; PF01381 HTH_3 [H]

EC number: NA

Molecular weight: Translated: 25037; Mature: 25037

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS50943 HTH_CROC1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELCDVFRNSMNSVMGHRKSFHSGIVFTMVKGRAEVASTEDEAASEHDRAFGSSIREARL
CCHHHHHHHHHHHHHHHHHHHHCCHHEEEECCCHHHHCCCHHHHHHHHHHHHHHHHHHHH
ARGLSLQRVADAAGLSVGLLSQVERGISSPSVRVLRAICGALGVTVQSLFGEDGAPLSES
HHCCHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHC
RHVMRPYQRRRLDLGAKGIVKEFLSRSDLSTLQIMEIILAPGGSSGEEAYNHIGEEGGVV
HHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHCCCCCEE
VEGAMELVIDGTVHRLMQGDSFAFESALPHKFRNLSEGVTRVLWITTPPVW
EECHHHHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHEEEEEECCCCC
>Mature Secondary Structure
MELCDVFRNSMNSVMGHRKSFHSGIVFTMVKGRAEVASTEDEAASEHDRAFGSSIREARL
CCHHHHHHHHHHHHHHHHHHHHCCHHEEEECCCHHHHCCCHHHHHHHHHHHHHHHHHHHH
ARGLSLQRVADAAGLSVGLLSQVERGISSPSVRVLRAICGALGVTVQSLFGEDGAPLSES
HHCCHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHC
RHVMRPYQRRRLDLGAKGIVKEFLSRSDLSTLQIMEIILAPGGSSGEEAYNHIGEEGGVV
HHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHCCCCCEE
VEGAMELVIDGTVHRLMQGDSFAFESALPHKFRNLSEGVTRVLWITTPPVW
EECHHHHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1840553 [H]