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The map label for this gene is hemB [H]

Identifier: 158423167

GI number: 158423167

Start: 1754931

End: 1755860

Strand: Reverse

Name: hemB [H]

Synonym: AZC_1543

Alternate gene names: 158423167

Gene position: 1755860-1754931 (Counterclockwise)

Preceding gene: 158423172

Following gene: 158423166

Centisome position: 32.7

GC content: 67.1

Gene sequence:

>930_bases
GTGCGCGAACACACGCTCACCGTCGATGACCTGATCTGGCCGATCTTCGTCGTCGAAGGCGAGAAGGTGCGCGAGAATGT
GGCCTCCATGCCGGGCGTCGAGCGCCTGTCCATCGACGAGGCGGTGCGCGCAGCGGAAATGGCGGCAAAGCTGCGCATTC
CCGCCCTCGCGCTCTTCCCCTACACAGAGCCGGGCCTGCGCGATCCGCACGGCTCCGAGGCGCTGAACGACAACAATCTC
GTCTGCCGCGCGCTGCGCGCCATCAAGGCCCGCGTGCCGGAGATCGGCCTCATCACCGACGTGGCGCTCGATCCCTACAC
CAGCCATGGCCATGACGGCCTCATGGACGGCGAGCGCATCGTCAATGACGCGACGGTGGAAGTGCTGGTGCGCCAGTCCA
TCGTCCAGGCCCAATGCGGAGCCGATGTGATCGCCCCCTCCGACATGATGGATGGCCGCGTCGGCGCCATCCGCCTCGGC
CTCGACAAGGCGGGCTTCGAGGACGTGCAGATCATGTCCTATGCGGCCAAGTACGCGTCGGCCTTCTATGGTCCGTTCCG
CGATGCCATCGGCACCTCCAAGACCCTCATCGGCGACAAGCGCACCTATCAGATGGACCCCGCCAATGGCGCGGAAGCCA
TCCGGGAAGCCGCCCTTGATGTGGAGGAGGGCGCCGACATGCTGATGGTGAAGCCCGGCCTGCCCTATCTGGACGTGGTC
TATCGCCTGAAGGAGGCCTTCGCGCTGCCCACCTATGCCTATCAGGTGTCCGGCGAGTACGCGATGATCGAGGGCGCCAT
CCGCAACGGCTGGCTCGACGGCAGCCGCGTGGTGGAGGAGAGCCTCATCGCCTTCAAGCGGGCGGGTGCCGACGGCATCC
TCACCTACTTCGCCCCGCGCGTGGCCGAGCGGCTCGCCGCCGGCGCGTGA

Upstream 100 bases:

>100_bases
CCTCCACTCCATCGATCCCGCGAAGGCAGCGGGCGGCCAGTCCCTCGATCTCGTCACCCGCCCCCGCCGCAACCGCAAGA
GCGACTGGAGCCGGCGGCTG

Downstream 100 bases:

>100_bases
CACCGGCGGCGCGCCTTCCGCTTTGCCTCTGGCCGTTGCTCGCGCTGCTGTGCGCGGGCTGCGGTTCGGTGGTGGACGTG
GATCAGGCGCGCCTGTGCGA

Product: delta-aminolevulinic acid dehydratase

Products: NA

Alternate protein names: ALAD; ALADH; Porphobilinogen synthase [H]

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MREHTLTVDDLIWPIFVVEGEKVRENVASMPGVERLSIDEAVRAAEMAAKLRIPALALFPYTEPGLRDPHGSEALNDNNL
VCRALRAIKARVPEIGLITDVALDPYTSHGHDGLMDGERIVNDATVEVLVRQSIVQAQCGADVIAPSDMMDGRVGAIRLG
LDKAGFEDVQIMSYAAKYASAFYGPFRDAIGTSKTLIGDKRTYQMDPANGAEAIREAALDVEEGADMLMVKPGLPYLDVV
YRLKEAFALPTYAYQVSGEYAMIEGAIRNGWLDGSRVVEESLIAFKRAGADGILTYFAPRVAERLAAGA

Sequences:

>Translated_309_residues
MREHTLTVDDLIWPIFVVEGEKVRENVASMPGVERLSIDEAVRAAEMAAKLRIPALALFPYTEPGLRDPHGSEALNDNNL
VCRALRAIKARVPEIGLITDVALDPYTSHGHDGLMDGERIVNDATVEVLVRQSIVQAQCGADVIAPSDMMDGRVGAIRLG
LDKAGFEDVQIMSYAAKYASAFYGPFRDAIGTSKTLIGDKRTYQMDPANGAEAIREAALDVEEGADMLMVKPGLPYLDVV
YRLKEAFALPTYAYQVSGEYAMIEGAIRNGWLDGSRVVEESLIAFKRAGADGILTYFAPRVAERLAAGA
>Mature_309_residues
MREHTLTVDDLIWPIFVVEGEKVRENVASMPGVERLSIDEAVRAAEMAAKLRIPALALFPYTEPGLRDPHGSEALNDNNL
VCRALRAIKARVPEIGLITDVALDPYTSHGHDGLMDGERIVNDATVEVLVRQSIVQAQCGADVIAPSDMMDGRVGAIRLG
LDKAGFEDVQIMSYAAKYASAFYGPFRDAIGTSKTLIGDKRTYQMDPANGAEAIREAALDVEEGADMLMVKPGLPYLDVV
YRLKEAFALPTYAYQVSGEYAMIEGAIRNGWLDGSRVVEESLIAFKRAGADGILTYFAPRVAERLAAGA

Specific function: Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen. Required for nodule development [H]

COG id: COG0113

COG function: function code H; Delta-aminolevulinic acid dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ALADH family [H]

Homologues:

Organism=Homo sapiens, GI189083849, Length=317, Percent_Identity=40.0630914826498, Blast_Score=206, Evalue=3e-53,
Organism=Escherichia coli, GI87081728, Length=302, Percent_Identity=48.6754966887417, Blast_Score=248, Evalue=2e-67,
Organism=Saccharomyces cerevisiae, GI6321398, Length=308, Percent_Identity=36.6883116883117, Blast_Score=176, Evalue=4e-45,
Organism=Drosophila melanogaster, GI21358291, Length=291, Percent_Identity=38.4879725085911, Blast_Score=217, Evalue=1e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001731
- InterPro:   IPR013785 [H]

Pfam domain/function: PF00490 ALAD [H]

EC number: =4.2.1.24 [H]

Molecular weight: Translated: 33563; Mature: 33563

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: PS00169 D_ALA_DEHYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MREHTLTVDDLIWPIFVVEGEKVRENVASMPGVERLSIDEAVRAAEMAAKLRIPALALFP
CCCCEEEHHHHHEEEEEECCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEC
YTEPGLRDPHGSEALNDNNLVCRALRAIKARVPEIGLITDVALDPYTSHGHDGLMDGERI
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCHHH
VNDATVEVLVRQSIVQAQCGADVIAPSDMMDGRVGAIRLGLDKAGFEDVQIMSYAAKYAS
HHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHH
AFYGPFRDAIGTSKTLIGDKRTYQMDPANGAEAIREAALDVEEGADMLMVKPGLPYLDVV
HHHHHHHHHCCCCCCEECCCCEEEECCCCHHHHHHHHHHHHHCCCCEEEECCCCCHHHHH
YRLKEAFALPTYAYQVSGEYAMIEGAIRNGWLDGSRVVEESLIAFKRAGADGILTYFAPR
HHHHHHHCCCCEEEEECCCEEEEEHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHH
VAERLAAGA
HHHHHHCCC
>Mature Secondary Structure
MREHTLTVDDLIWPIFVVEGEKVRENVASMPGVERLSIDEAVRAAEMAAKLRIPALALFP
CCCCEEEHHHHHEEEEEECCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEC
YTEPGLRDPHGSEALNDNNLVCRALRAIKARVPEIGLITDVALDPYTSHGHDGLMDGERI
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCHHH
VNDATVEVLVRQSIVQAQCGADVIAPSDMMDGRVGAIRLGLDKAGFEDVQIMSYAAKYAS
HHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHH
AFYGPFRDAIGTSKTLIGDKRTYQMDPANGAEAIREAALDVEEGADMLMVKPGLPYLDVV
HHHHHHHHHCCCCCCEECCCCEEEECCCCHHHHHHHHHHHHHCCCCEEEECCCCCHHHHH
YRLKEAFALPTYAYQVSGEYAMIEGAIRNGWLDGSRVVEESLIAFKRAGADGILTYFAPR
HHHHHHHCCCCEEEEECCCEEEEEHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHH
VAERLAAGA
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8226669; 12597275 [H]