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The map label for this gene is purU [H]

Identifier: 158423116

GI number: 158423116

Start: 1692943

End: 1693812

Strand: Reverse

Name: purU [H]

Synonym: AZC_1492

Alternate gene names: 158423116

Gene position: 1693812-1692943 (Counterclockwise)

Preceding gene: 158423117

Following gene: 158423112

Centisome position: 31.54

GC content: 64.02

Gene sequence:

>870_bases
ATGCCCACGCCCAGCCAGACCTTTGTCCTGAAATTCTCCTGCCCCGACCGGGCCGGCATCGTTGCCGCCGTCTCGACCTT
TCTCTATGAGCAGGGCTGCAACATCCTGGAAGCCCAGCAGTTCGACGACACGGAGAGCAACCGCTTCTTCATGCGCGTCG
CCTTCAACGTGATCGAAGGCACGGCCGACCTTCCGCGGATCCGCACCGCCTTCGGTCCGGTGGCGGACGGCTTCAAGCTC
GACTGGTCCATGCGCGATCCGGCCGAAAAGCGCCGCGTGCTGCTGCTGGTCTCGAAGTTCGATCACTGCCTCGCCGATCT
GCTCTATCGCTGGCGCATCGGCGAGATCCCCATGGAGATCACGGGCATCATCTCCAACCACCCCATCGAGACCTACGCGC
ATCTCGATTTCGACGGCATCCCCTTCCATCATCTGCCGGTGAGCAAGGCCACCAAGATGGAGCAGGAGGCGCAGGTCTGG
CGCATCTTCCAGGAGAGCGGCTCGGAAATGGCGGTGCTCGCCCGCTACATGCAGGTGCTCTCCGATGGGCTGTCCGCCAA
GCTCTCGGGGCGCTGCATCAACATCCACCATTCCTTCCTGCCCGGCTTCAAGGGCGCCAAGCCCTATCATCAGGCCCACC
AGCGCGGCGTGAAGCTGATCGGCGCCACCGCCCATTATGTGACGTCCGACCTTGATGAAGGCCCGATCATCGAGCAGGAC
GTGGAGCGCATCACCCATCAGGACAGCCCGGACGATCTGGTGCGCAAGGGCCGCGATATCGAGCGCCGCGTGCTCGCCCG
CGCGCTCGCCTGGCACCTGCAAGACCGGGTGATCCCGAACGGCCACAAGACCATCGTCTTCCGCGACTGA

Upstream 100 bases:

>100_bases
CCCCCTTCCCGCCGGGGCTTCACCCCCGTAATGACCTCCCCCAAGGGTGCGGCGCCCGGCCGTCGGGCGCCCGCGCCGGT
CGATATGTGCGGAACATCCC

Downstream 100 bases:

>100_bases
CGCGACCGATACAGGAGCCCACACGGAAAAGCCCGGCCCGCGCCTGCGGACCGGGCTTTTCCTTTGTCATCAGCACGAAA
GGCTCAGGCCTTGTTAGCCT

Product: formyltetrahydrofolate deformylase

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase [H]

Number of amino acids: Translated: 289; Mature: 288

Protein sequence:

>289_residues
MPTPSQTFVLKFSCPDRAGIVAAVSTFLYEQGCNILEAQQFDDTESNRFFMRVAFNVIEGTADLPRIRTAFGPVADGFKL
DWSMRDPAEKRRVLLLVSKFDHCLADLLYRWRIGEIPMEITGIISNHPIETYAHLDFDGIPFHHLPVSKATKMEQEAQVW
RIFQESGSEMAVLARYMQVLSDGLSAKLSGRCINIHHSFLPGFKGAKPYHQAHQRGVKLIGATAHYVTSDLDEGPIIEQD
VERITHQDSPDDLVRKGRDIERRVLARALAWHLQDRVIPNGHKTIVFRD

Sequences:

>Translated_289_residues
MPTPSQTFVLKFSCPDRAGIVAAVSTFLYEQGCNILEAQQFDDTESNRFFMRVAFNVIEGTADLPRIRTAFGPVADGFKL
DWSMRDPAEKRRVLLLVSKFDHCLADLLYRWRIGEIPMEITGIISNHPIETYAHLDFDGIPFHHLPVSKATKMEQEAQVW
RIFQESGSEMAVLARYMQVLSDGLSAKLSGRCINIHHSFLPGFKGAKPYHQAHQRGVKLIGATAHYVTSDLDEGPIIEQD
VERITHQDSPDDLVRKGRDIERRVLARALAWHLQDRVIPNGHKTIVFRD
>Mature_288_residues
PTPSQTFVLKFSCPDRAGIVAAVSTFLYEQGCNILEAQQFDDTESNRFFMRVAFNVIEGTADLPRIRTAFGPVADGFKLD
WSMRDPAEKRRVLLLVSKFDHCLADLLYRWRIGEIPMEITGIISNHPIETYAHLDFDGIPFHHLPVSKATKMEQEAQVWR
IFQESGSEMAVLARYMQVLSDGLSAKLSGRCINIHHSFLPGFKGAKPYHQAHQRGVKLIGATAHYVTSDLDEGPIIEQDV
ERITHQDSPDDLVRKGRDIERRVLARALAWHLQDRVIPNGHKTIVFRD

Specific function: Produces Formate From Formyl-Tetrahydrofolate. Provides The Major Source Of Formate For The Purt-Dependent Synthesis Of 5'-Phosphoribosyl-N-Formylglycinamide (Fgar) During Aerobic Growth. Has A Role In Regulating The One-Carbon Pool. [C]

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=188, Percent_Identity=30.3191489361702, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI209869995, Length=188, Percent_Identity=30.3191489361702, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI209869993, Length=188, Percent_Identity=30.3191489361702, Blast_Score=76, Evalue=3e-14,
Organism=Escherichia coli, GI1787483, Length=288, Percent_Identity=40.625, Blast_Score=209, Evalue=2e-55,
Organism=Escherichia coli, GI1788846, Length=109, Percent_Identity=32.1100917431193, Blast_Score=67, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17567511, Length=154, Percent_Identity=30.5194805194805, Blast_Score=77, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR004810 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =3.5.1.10 [H]

Molecular weight: Translated: 32820; Mature: 32689

Theoretical pI: Translated: 7.00; Mature: 7.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTPSQTFVLKFSCPDRAGIVAAVSTFLYEQGCNILEAQQFDDTESNRFFMRVAFNVIEG
CCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCEEECCCCCCCCCCEEEEEEHHHHHHC
TADLPRIRTAFGPVADGFKLDWSMRDPAEKRRVLLLVSKFDHCLADLLYRWRIGEIPMEI
CCCCCHHHHHHCCCCCCCEEECCCCCHHHHHHEEEHHHHHHHHHHHHHHHHCCCCCCEEE
TGIISNHPIETYAHLDFDGIPFHHLPVSKATKMEQEAQVWRIFQESGSEMAVLARYMQVL
EEECCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
SDGLSAKLSGRCINIHHSFLPGFKGAKPYHQAHQRGVKLIGATAHYVTSDLDEGPIIEQD
HCCCCCEECCCEEEEEHHHCCCCCCCCCHHHHHHCCCEEEECHHHHHHCCCCCCCCCHHH
VERITHQDSPDDLVRKGRDIERRVLARALAWHLQDRVIPNGHKTIVFRD
HHHHHCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEC
>Mature Secondary Structure 
PTPSQTFVLKFSCPDRAGIVAAVSTFLYEQGCNILEAQQFDDTESNRFFMRVAFNVIEG
CCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCEEECCCCCCCCCCEEEEEEHHHHHHC
TADLPRIRTAFGPVADGFKLDWSMRDPAEKRRVLLLVSKFDHCLADLLYRWRIGEIPMEI
CCCCCHHHHHHCCCCCCCEEECCCCCHHHHHHEEEHHHHHHHHHHHHHHHHCCCCCCEEE
TGIISNHPIETYAHLDFDGIPFHHLPVSKATKMEQEAQVWRIFQESGSEMAVLARYMQVL
EEECCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
SDGLSAKLSGRCINIHHSFLPGFKGAKPYHQAHQRGVKLIGATAHYVTSDLDEGPIIEQD
HCCCCCEECCCEEEEEHHHCCCCCCCCCHHHHHHCCCEEEECHHHHHHCCCCCCCCCHHH
VERITHQDSPDDLVRKGRDIERRVLARALAWHLQDRVIPNGHKTIVFRD
HHHHHCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7543100 [H]