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The map label for this gene is rnhA [H]

Identifier: 158423090

GI number: 158423090

Start: 1665471

End: 1665923

Strand: Reverse

Name: rnhA [H]

Synonym: AZC_1466

Alternate gene names: 158423090

Gene position: 1665923-1665471 (Counterclockwise)

Preceding gene: 158423091

Following gene: 158423086

Centisome position: 31.02

GC content: 67.33

Gene sequence:

>453_bases
ATGAGCCGGGTGGAGATCTGGACCGACGGCGCCTGCTCGGGCAATCCCGGCCCCGGCGGCTGGGGCGCCATCCTGCGCTC
CGGTCCGCACGAGAAGGAACTGAAGGGCGGCGAGGCTCTCACCACCAACAACCGCATGGAGCTGATGGCCGCCATCTCGG
CGCTGGAAGCGCTGAAGAAGCCCTGCGGGGTCGATCTTCACACCGACAGCGAATATCTGCGCAACGGCATCACCAAGTGG
ATGTTCGGCTGGAAGCGCAACGGCTGGCGCACCGCCGACAAGAAGCCGGTGAAGAATCAGGATCTGTGGGAGCGCCTCGA
CGCCGCCCTGCACTCCCACGACATCGCCTGGCACTGGGTGAAGGGCCACGCGGGCAACGAACTGAACGAGCGCGCAGACC
AGCTCGCCCGCGACGGCATGGCCCCCTTCAAGATGGGGGGCCGCGTCGCCTGA

Upstream 100 bases:

>100_bases
CGGAAGGCGCCCTCGTGCGCCCCAAAGACCCCTTGGAATATCTGAGGAAGCTACGCTTCCACCGGGCCGTAGCCAGCGCC
CGCGACTATGGACTTGCCGC

Downstream 100 bases:

>100_bases
GTTGAGGGCACACCTCGAAGAAGGGCCGCAGGCGATGCCGGCGGCCCTTCTCGTTTCACCTGAGTGAACGTTTCATTTGC
CGCGCTTGAAGGTGTTCCAG

Product: ribonuclease H

Products: NA

Alternate protein names: RNase H [H]

Number of amino acids: Translated: 150; Mature: 149

Protein sequence:

>150_residues
MSRVEIWTDGACSGNPGPGGWGAILRSGPHEKELKGGEALTTNNRMELMAAISALEALKKPCGVDLHTDSEYLRNGITKW
MFGWKRNGWRTADKKPVKNQDLWERLDAALHSHDIAWHWVKGHAGNELNERADQLARDGMAPFKMGGRVA

Sequences:

>Translated_150_residues
MSRVEIWTDGACSGNPGPGGWGAILRSGPHEKELKGGEALTTNNRMELMAAISALEALKKPCGVDLHTDSEYLRNGITKW
MFGWKRNGWRTADKKPVKNQDLWERLDAALHSHDIAWHWVKGHAGNELNERADQLARDGMAPFKMGGRVA
>Mature_149_residues
SRVEIWTDGACSGNPGPGGWGAILRSGPHEKELKGGEALTTNNRMELMAAISALEALKKPCGVDLHTDSEYLRNGITKWM
FGWKRNGWRTADKKPVKNQDLWERLDAALHSHDIAWHWVKGHAGNELNERADQLARDGMAPFKMGGRVA

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids [H]

COG id: COG0328

COG function: function code L; Ribonuclease HI

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RNase H domain [H]

Homologues:

Organism=Homo sapiens, GI21359816, Length=147, Percent_Identity=40.1360544217687, Blast_Score=82, Evalue=2e-16,
Organism=Escherichia coli, GI1786408, Length=137, Percent_Identity=66.4233576642336, Blast_Score=189, Evalue=5e-50,
Organism=Caenorhabditis elegans, GI115532542, Length=143, Percent_Identity=37.0629370629371, Blast_Score=88, Evalue=2e-18,
Organism=Drosophila melanogaster, GI45552509, Length=152, Percent_Identity=37.5, Blast_Score=94, Evalue=4e-20,
Organism=Drosophila melanogaster, GI17137476, Length=152, Percent_Identity=37.5, Blast_Score=94, Evalue=5e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022892
- InterPro:   IPR012337
- InterPro:   IPR002156 [H]

Pfam domain/function: PF00075 RnaseH [H]

EC number: =3.1.26.4 [H]

Molecular weight: Translated: 16658; Mature: 16527

Theoretical pI: Translated: 8.76; Mature: 8.76

Prosite motif: PS50879 RNASE_H

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRVEIWTDGACSGNPGPGGWGAILRSGPHEKELKGGEALTTNNRMELMAAISALEALKK
CCCEEEEECCCCCCCCCCCHHHHHHCCCCCHHHCCCCCEECCCCHHHHHHHHHHHHHHHH
PCGVDLHTDSEYLRNGITKWMFGWKRNGWRTADKKPVKNQDLWERLDAALHSHDIAWHWV
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEE
KGHAGNELNERADQLARDGMAPFKMGGRVA
CCCCCCHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
SRVEIWTDGACSGNPGPGGWGAILRSGPHEKELKGGEALTTNNRMELMAAISALEALKK
CCEEEEECCCCCCCCCCCHHHHHHCCCCCHHHCCCCCEECCCCHHHHHHHHHHHHHHHH
PCGVDLHTDSEYLRNGITKWMFGWKRNGWRTADKKPVKNQDLWERLDAALHSHDIAWHWV
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEE
KGHAGNELNERADQLARDGMAPFKMGGRVA
CCCCCCHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]