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The map label for this gene is emrB [H]

Identifier: 158318144

GI number: 158318144

Start: 7779067

End: 7780803

Strand: Reverse

Name: emrB [H]

Synonym: Franean1_6409

Alternate gene names: 158318144

Gene position: 7780803-7779067 (Counterclockwise)

Preceding gene: 158318146

Following gene: 158318142

Centisome position: 86.63

GC content: 71.16

Gene sequence:

>1737_bases
GTGAGCAGTGATCTGGGCAGCTTGGCGGGTCCGGAGGAGGCAGGGGAACCACAGGAGGCAGGGGAGCTACGAGCGGAAGG
GGAAAGGGAAGGGATCCGAGGGGAGCCGGGTGGGCGATCCGTGCCATCCGGCTCGGCCGGGCCGCCGCACACCGGGCTGG
TGTTCACGGTGCTCGTGCTCGGTGGCCTGCTCGTCGTTCTGGATATCACGATCATCAATGTCGCGATCCGCACGCTGGCG
GCGGATCTCGATGCTTCACTGCCGGTGATCCAGTGGGTTTCGACGGGATACACCCTGGCGCTGGCCGTGACCGTGCCGAC
GACAGCATGGCTGGTGGCACGGTTCGGTTCCGGACGTGTCTACATGGTGGCCCTGAGTCTGTTCGTGCTGGGGTCGGTGC
TCTGCGGTCTGGCCTGGAACATCGACGCGCTCATTGCCTTCCGGGTGGTGCAGGGGATCGGCGGTGGCCTGGTCAACCCG
GTCGCGATGACGATTGTGCTACGGGCCACGCCGCCGGAGCGGCGCGGGCGCGCCATGGGCCTGTTGGGTCTGCCGGTGCT
CGTCGGGCCGGTGATCGGGCCGACGCTGGGCGGCTGGCTGGTCGACATCTCCTGGCGGTGGATCTTCCTGGTCAACCTGC
CGCTGGGCCTGGCCGCGCTCCTGCTCGCGAGCCGAGTCCTGCGCCCGGTCACCGCGGCCGCGGGTGCCGTCCAGCGGGGC
GGCACGCTTGAGAGTGCTGACGGGCGGCTCGACGTCCCAGGGTTGGCGCTTGTCGCGCCGGGGCTGGCGCTGTTCGTCTA
CGGGCTGGCGGAGAGCGGGCGGCGCGGGACTGTGACCTCGGCAGGTGTGCTCGTGCCGGCGCTGGCCGGGCTCGCGTTGG
CGGTGGTGTTCGTGGTTCGGGCCGCTCGGATGCGCGCCCCACTGGTCCAGGTTGCGCTGTTGCGGCTGCGGGCTGTCGCG
TCAGGGACGGCGACGCTGGCGCTGTTCGCGGCGGCCTACTTCGGTTCGATGTTCGTCCTGCCGCTTTACTGGCAGCTCGT
GCGGGGGCTCAGCCCCGCGGAGACGGGGATGCTGGCGATCCCGCAGGCGCTCGCTACCGGGGCTTCGCTGCAGGTGGCGA
GCCGGATGGTCGACCGGGTTCCGCCTGCCCGCGTGGTGGGCTTCGGGATCGTGACGGCGTCCTGCGGGCTGATCACCGCG
ACGCTGTTGCTCGGCGTTGACACCCCGTACTGGCAGATGGTGGTGGCGATGTCCGTCATGGGGGTCGGCGCGGGCTCGAC
GATCATGCCGACGATCACGACAGCGCTGCGGCATCTGAGTGACCGGGACGCGCCGTCCGGCAGCACGCTGCTGACCATCA
CCAACCAGGTGAGTGTCTCGATCGGAACCGCCCTGACCTCCGTCGTACTCGCGGCCGGCCTCACCACCCAGGGCGTGGCC
GGTGCGGCCGGTGGCGGCGGCGAGGGCGTGCTGCCGACCGTGGTGGACGGCCCGGCCGCCGCCCGGCTTGCCGAGGCCTG
CCAGGACACGCTGTTCGTGTCCGCCGCGCTGCTGGTCGCCGCGCTCGTCGTGGCGCTGACAGCGATCCCGGGCGGTCCGT
TCAGTGCTTCTCGACGTCGCCGGGTCCAGCCGCGTATCCGTGCTCCTCGACGTCGCCGGGTTCGGCCTCGCGGGGAGGCG
CAGGATCAGTCGCCGGTTCGAGCAGCGCGGACAGGCGATCTGACTCCGCCGCGGTGA

Upstream 100 bases:

>100_bases
GGGGCTGGGGTTGACCTCTACTTCGGTTTAGGTGTGAGGCTGGGGTGGTCGGTGCCGGGTGGTGCCCTTGGCGGGGCCGG
GTGGGAGGGATGGCGCGGTT

Downstream 100 bases:

>100_bases
GCTCCCGCAGCAGCGGGAGGCTGGCGAGCAGATCGGTGACCGGGAGCCTGTTCGGGCCGCGCGGCGCGGCGACCTGGGTC
GTGAAGTCGCAGACGAGACT

Product: EmrB/QacA family drug resistance transporter

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 578; Mature: 577

Protein sequence:

>578_residues
MSSDLGSLAGPEEAGEPQEAGELRAEGEREGIRGEPGGRSVPSGSAGPPHTGLVFTVLVLGGLLVVLDITIINVAIRTLA
ADLDASLPVIQWVSTGYTLALAVTVPTTAWLVARFGSGRVYMVALSLFVLGSVLCGLAWNIDALIAFRVVQGIGGGLVNP
VAMTIVLRATPPERRGRAMGLLGLPVLVGPVIGPTLGGWLVDISWRWIFLVNLPLGLAALLLASRVLRPVTAAAGAVQRG
GTLESADGRLDVPGLALVAPGLALFVYGLAESGRRGTVTSAGVLVPALAGLALAVVFVVRAARMRAPLVQVALLRLRAVA
SGTATLALFAAAYFGSMFVLPLYWQLVRGLSPAETGMLAIPQALATGASLQVASRMVDRVPPARVVGFGIVTASCGLITA
TLLLGVDTPYWQMVVAMSVMGVGAGSTIMPTITTALRHLSDRDAPSGSTLLTITNQVSVSIGTALTSVVLAAGLTTQGVA
GAAGGGGEGVLPTVVDGPAAARLAEACQDTLFVSAALLVAALVVALTAIPGGPFSASRRRRVQPRIRAPRRRRVRPRGEA
QDQSPVRAARTGDLTPPR

Sequences:

>Translated_578_residues
MSSDLGSLAGPEEAGEPQEAGELRAEGEREGIRGEPGGRSVPSGSAGPPHTGLVFTVLVLGGLLVVLDITIINVAIRTLA
ADLDASLPVIQWVSTGYTLALAVTVPTTAWLVARFGSGRVYMVALSLFVLGSVLCGLAWNIDALIAFRVVQGIGGGLVNP
VAMTIVLRATPPERRGRAMGLLGLPVLVGPVIGPTLGGWLVDISWRWIFLVNLPLGLAALLLASRVLRPVTAAAGAVQRG
GTLESADGRLDVPGLALVAPGLALFVYGLAESGRRGTVTSAGVLVPALAGLALAVVFVVRAARMRAPLVQVALLRLRAVA
SGTATLALFAAAYFGSMFVLPLYWQLVRGLSPAETGMLAIPQALATGASLQVASRMVDRVPPARVVGFGIVTASCGLITA
TLLLGVDTPYWQMVVAMSVMGVGAGSTIMPTITTALRHLSDRDAPSGSTLLTITNQVSVSIGTALTSVVLAAGLTTQGVA
GAAGGGGEGVLPTVVDGPAAARLAEACQDTLFVSAALLVAALVVALTAIPGGPFSASRRRRVQPRIRAPRRRRVRPRGEA
QDQSPVRAARTGDLTPPR
>Mature_577_residues
SSDLGSLAGPEEAGEPQEAGELRAEGEREGIRGEPGGRSVPSGSAGPPHTGLVFTVLVLGGLLVVLDITIINVAIRTLAA
DLDASLPVIQWVSTGYTLALAVTVPTTAWLVARFGSGRVYMVALSLFVLGSVLCGLAWNIDALIAFRVVQGIGGGLVNPV
AMTIVLRATPPERRGRAMGLLGLPVLVGPVIGPTLGGWLVDISWRWIFLVNLPLGLAALLLASRVLRPVTAAAGAVQRGG
TLESADGRLDVPGLALVAPGLALFVYGLAESGRRGTVTSAGVLVPALAGLALAVVFVVRAARMRAPLVQVALLRLRAVAS
GTATLALFAAAYFGSMFVLPLYWQLVRGLSPAETGMLAIPQALATGASLQVASRMVDRVPPARVVGFGIVTASCGLITAT
LLLGVDTPYWQMVVAMSVMGVGAGSTIMPTITTALRHLSDRDAPSGSTLLTITNQVSVSIGTALTSVVLAAGLTTQGVAG
AAGGGGEGVLPTVVDGPAAARLAEACQDTLFVSAALLVAALVVALTAIPGGPFSASRRRRVQPRIRAPRRRRVRPRGEAQ
DQSPVRAARTGDLTPPR

Specific function: Unknown

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1790195, Length=412, Percent_Identity=31.3106796116505, Blast_Score=178, Evalue=9e-46,
Organism=Escherichia coli, GI1789042, Length=415, Percent_Identity=28.6746987951807, Blast_Score=148, Evalue=1e-36,
Organism=Escherichia coli, GI87081983, Length=359, Percent_Identity=31.7548746518106, Blast_Score=145, Evalue=6e-36,
Organism=Escherichia coli, GI1788710, Length=412, Percent_Identity=26.6990291262136, Blast_Score=139, Evalue=6e-34,
Organism=Escherichia coli, GI1788392, Length=421, Percent_Identity=30.166270783848, Blast_Score=132, Evalue=5e-32,
Organism=Saccharomyces cerevisiae, GI6325455, Length=454, Percent_Identity=23.1277533039648, Blast_Score=80, Evalue=9e-16,
Organism=Saccharomyces cerevisiae, GI6322958, Length=422, Percent_Identity=22.9857819905213, Blast_Score=77, Evalue=8e-15,
Organism=Saccharomyces cerevisiae, GI6321663, Length=150, Percent_Identity=30.6666666666667, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 59156; Mature: 59025

Theoretical pI: Translated: 11.51; Mature: 11.51

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSDLGSLAGPEEAGEPQEAGELRAEGEREGIRGEPGGRSVPSGSAGPPHTGLVFTVLVL
CCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
GGLLVVLDITIINVAIRTLAADLDASLPVIQWVSTGYTLALAVTVPTTAWLVARFGSGRV
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCEEEEEEEECCHHHHHHHHCCCCCH
YMVALSLFVLGSVLCGLAWNIDALIAFRVVQGIGGGLVNPVAMTIVLRATPPERRGRAMG
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCHHHCCCCC
LLGLPVLVGPVIGPTLGGWLVDISWRWIFLVNLPLGLAALLLASRVLRPVTAAAGAVQRG
HHHHHHHHHHHHCCCCCCEEEEEEEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHCC
GTLESADGRLDVPGLALVAPGLALFVYGLAESGRRGTVTSAGVLVPALAGLALAVVFVVR
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
AARMRAPLVQVALLRLRAVASGTATLALFAAAYFGSMFVLPLYWQLVRGLSPAETGMLAI
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
PQALATGASLQVASRMVDRVPPARVVGFGIVTASCGLITATLLLGVDTPYWQMVVAMSVM
HHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
GVGAGSTIMPTITTALRHLSDRDAPSGSTLLTITNQVSVSIGTALTSVVLAAGLTTQGVA
CCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCCCCC
GAAGGGGEGVLPTVVDGPAAARLAEACQDTLFVSAALLVAALVVALTAIPGGPFSASRRR
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
RVQPRIRAPRRRRVRPRGEAQDQSPVRAARTGDLTPPR
CCCCHHCCCHHHCCCCCCCCCCCCCCHHHCCCCCCCCC
>Mature Secondary Structure 
SSDLGSLAGPEEAGEPQEAGELRAEGEREGIRGEPGGRSVPSGSAGPPHTGLVFTVLVL
CCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
GGLLVVLDITIINVAIRTLAADLDASLPVIQWVSTGYTLALAVTVPTTAWLVARFGSGRV
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCEEEEEEEECCHHHHHHHHCCCCCH
YMVALSLFVLGSVLCGLAWNIDALIAFRVVQGIGGGLVNPVAMTIVLRATPPERRGRAMG
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCHHHCCCCC
LLGLPVLVGPVIGPTLGGWLVDISWRWIFLVNLPLGLAALLLASRVLRPVTAAAGAVQRG
HHHHHHHHHHHHCCCCCCEEEEEEEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHCC
GTLESADGRLDVPGLALVAPGLALFVYGLAESGRRGTVTSAGVLVPALAGLALAVVFVVR
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
AARMRAPLVQVALLRLRAVASGTATLALFAAAYFGSMFVLPLYWQLVRGLSPAETGMLAI
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
PQALATGASLQVASRMVDRVPPARVVGFGIVTASCGLITATLLLGVDTPYWQMVVAMSVM
HHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
GVGAGSTIMPTITTALRHLSDRDAPSGSTLLTITNQVSVSIGTALTSVVLAAGLTTQGVA
CCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCCCCC
GAAGGGGEGVLPTVVDGPAAARLAEACQDTLFVSAALLVAALVVALTAIPGGPFSASRRR
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
RVQPRIRAPRRRRVRPRGEAQDQSPVRAARTGDLTPPR
CCCCHHCCCHHHCCCCCCCCCCCCCCHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]