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The map label for this gene is folC [H]

Identifier: 158317027

GI number: 158317027

Start: 6339986

End: 6341392

Strand: Reverse

Name: folC [H]

Synonym: Franean1_5271

Alternate gene names: 158317027

Gene position: 6341392-6339986 (Counterclockwise)

Preceding gene: 158317028

Following gene: 158317026

Centisome position: 70.6

GC content: 73.56

Gene sequence:

>1407_bases
GTGCCCGCGCCGGCCGCGCCCTCGGAACGGGCGTCCCGATGCCATAACCTCGGCTCCGTGACAGACGCACGCACGGGGCG
CACGCCGGACGACGCGGCCGAGCTCGCCCGGGTGGAGAAGGCACTGGCCGGGCGGTTCCCGCACCGGATGCACCCGGACC
TGGACCGGATGCGGGATCTCGTCGACCTGCTGGGGCACCCGGAGCGGTCCTTCCCGTCCATCCACATCACCGGGACGAAC
GGCAAGACGTCCACGGCTCGGATGATCGACGCGCTGCTTCGGGGCTTCGGGCTGCGGCCGGGCCGGTACACCTCCCCGCA
CATGGAAAGCGTCACGGAGCGGATCAGCATCAACGGCGTGCCCGCCGACGCTGAGGTGTTCGCCAGGGCCTACGACGACG
TGATCCCCTACGCGGAGCTCGTCGACAGCCGCCATCCCGAGCGGGTCACGTTCTTCGAGCTGCTCACCGCGATGGCGTTC
TCCGCCTTCTCCGACTCGCCCGTGGACGCCGGGGTCGTCGAGGTCGGCATGGGCGGCACCTGGGACGCGACGAACGTGGT
CGACGCGGGCGTCGCGGTGATCACGCCGGTCTCCCTCGACCATCCGGAGCTGGGTGACACCGTGGAGGCGGTGGCGGCGG
AGAAGGCGGGGATCATCCGGCCGGACGCCCTGGTGGTGCTGGCGCAGCAGTCGCTGCCCGCCGCCGAGGTCCTGCTGCGG
CGCAGCGCCGAGGTCGGCGCCACCGTCGCGCGGGAGGGCCTGGAGTTCGGCGTCGTCGGCCGGCGGGTGGCGGTGGGGGG
CCAGGTGCTGACCCTGCGCGGTCTGGGCGGGACGTACGAGGAGGTCTTCCTGCCGCTGCACGGCATCCACCAGGCGCACA
ACGCGGCCGTCGCGCTGGCGGCCGTCGAGGCGTTCCTCGGCGGCGGGCAGGGTCAGCTGGACGCCGACGCCGTCCTCGCC
GGGTTCGCCCAGGCCGACTCGCCTGGCCGGCTGGAGGTCGTGCGGCGCTCGCCGACCATCGTCCTGGACGGCGCGCACAA
CGTCGCCGGCGCGCAGGCCCTCGCGGCCGCGGTCGACGAGGCCTTCGGCTTCGACAATCTCGTCGGGGTCGTCGGTGTGC
TCGGAGACAAGGACGCCGAGGGCATCCTCGCCGCCCTGGAACCGGTGCTCAGCAGCGTCGTCGTGACCCAGAGCACGTCA
CCGCGGGCGATGGGCGCGGACGACCTGGCCGCGATCGCGGTGGACGTCTTCGATGCCGACCGGGTCGAGGTCGCGCCACG
GCTGGACGACGCCCTCGAGGCCGCGGTGCGGCTCGCGGAGGAGGAGGGCGAGCTGGGCGGCACGGGTGTGCTGGTCACCG
GCTCGCTGGTGACCGTGGGTGAGGCGCGGCACCTGCTTCGTCGCTGA

Upstream 100 bases:

>100_bases
GTCGCCGCGTCGAAGCGCGGCCCGCGCCAGGAGAAGTCCGCCTGACGAGATTGCGGCCGGCCGCCCCGGCACCGGTCGCC
GTGGCCTCGGCGACGGCGAG

Downstream 100 bases:

>100_bases
CCGTCCGCCGGCCGGCGATGATCTGATGTGCCTGGTGATCCCAGTGTTGCCCGCGCGGTGCTCTGACGGGTGGTCGGGGG
ACTGCGCGGACCGGCGGTGA

Product: FolC bifunctional protein

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolate synthase; Tetrahydrofolylpolyglutamate synthase [H]

Number of amino acids: Translated: 468; Mature: 467

Protein sequence:

>468_residues
MPAPAAPSERASRCHNLGSVTDARTGRTPDDAAELARVEKALAGRFPHRMHPDLDRMRDLVDLLGHPERSFPSIHITGTN
GKTSTARMIDALLRGFGLRPGRYTSPHMESVTERISINGVPADAEVFARAYDDVIPYAELVDSRHPERVTFFELLTAMAF
SAFSDSPVDAGVVEVGMGGTWDATNVVDAGVAVITPVSLDHPELGDTVEAVAAEKAGIIRPDALVVLAQQSLPAAEVLLR
RSAEVGATVAREGLEFGVVGRRVAVGGQVLTLRGLGGTYEEVFLPLHGIHQAHNAAVALAAVEAFLGGGQGQLDADAVLA
GFAQADSPGRLEVVRRSPTIVLDGAHNVAGAQALAAAVDEAFGFDNLVGVVGVLGDKDAEGILAALEPVLSSVVVTQSTS
PRAMGADDLAAIAVDVFDADRVEVAPRLDDALEAAVRLAEEEGELGGTGVLVTGSLVTVGEARHLLRR

Sequences:

>Translated_468_residues
MPAPAAPSERASRCHNLGSVTDARTGRTPDDAAELARVEKALAGRFPHRMHPDLDRMRDLVDLLGHPERSFPSIHITGTN
GKTSTARMIDALLRGFGLRPGRYTSPHMESVTERISINGVPADAEVFARAYDDVIPYAELVDSRHPERVTFFELLTAMAF
SAFSDSPVDAGVVEVGMGGTWDATNVVDAGVAVITPVSLDHPELGDTVEAVAAEKAGIIRPDALVVLAQQSLPAAEVLLR
RSAEVGATVAREGLEFGVVGRRVAVGGQVLTLRGLGGTYEEVFLPLHGIHQAHNAAVALAAVEAFLGGGQGQLDADAVLA
GFAQADSPGRLEVVRRSPTIVLDGAHNVAGAQALAAAVDEAFGFDNLVGVVGVLGDKDAEGILAALEPVLSSVVVTQSTS
PRAMGADDLAAIAVDVFDADRVEVAPRLDDALEAAVRLAEEEGELGGTGVLVTGSLVTVGEARHLLRR
>Mature_467_residues
PAPAAPSERASRCHNLGSVTDARTGRTPDDAAELARVEKALAGRFPHRMHPDLDRMRDLVDLLGHPERSFPSIHITGTNG
KTSTARMIDALLRGFGLRPGRYTSPHMESVTERISINGVPADAEVFARAYDDVIPYAELVDSRHPERVTFFELLTAMAFS
AFSDSPVDAGVVEVGMGGTWDATNVVDAGVAVITPVSLDHPELGDTVEAVAAEKAGIIRPDALVVLAQQSLPAAEVLLRR
SAEVGATVAREGLEFGVVGRRVAVGGQVLTLRGLGGTYEEVFLPLHGIHQAHNAAVALAAVEAFLGGGQGQLDADAVLAG
FAQADSPGRLEVVRRSPTIVLDGAHNVAGAQALAAAVDEAFGFDNLVGVVGVLGDKDAEGILAALEPVLSSVVVTQSTSP
RAMGADDLAAIAVDVFDADRVEVAPRLDDALEAAVRLAEEEGELGGTGVLVTGSLVTVGEARHLLRR

Specific function: Conversion of folates to polyglutamate derivatives. It preferes 5,10-methylenetetrahydrofolate, rather than 10- formyltetrahydrofolate as folate substrate [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932990, Length=354, Percent_Identity=32.7683615819209, Blast_Score=149, Evalue=8e-36,
Organism=Homo sapiens, GI66932984, Length=354, Percent_Identity=32.7683615819209, Blast_Score=148, Evalue=9e-36,
Organism=Escherichia coli, GI1788654, Length=386, Percent_Identity=30.5699481865285, Blast_Score=152, Evalue=6e-38,
Organism=Caenorhabditis elegans, GI17553148, Length=435, Percent_Identity=26.2068965517241, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17553150, Length=435, Percent_Identity=26.2068965517241, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI71984923, Length=435, Percent_Identity=26.2068965517241, Blast_Score=117, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6323760, Length=365, Percent_Identity=30.1369863013699, Blast_Score=155, Evalue=1e-38,
Organism=Saccharomyces cerevisiae, GI6324815, Length=297, Percent_Identity=29.2929292929293, Blast_Score=108, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24641571, Length=180, Percent_Identity=37.2222222222222, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24581568, Length=189, Percent_Identity=28.5714285714286, Blast_Score=89, Evalue=6e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 48806; Mature: 48674

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: PS01012 FOLYLPOLYGLU_SYNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAPAAPSERASRCHNLGSVTDARTGRTPDDAAELARVEKALAGRFPHRMHPDLDRMRDL
CCCCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
VDLLGHPERSFPSIHITGTNGKTSTARMIDALLRGFGLRPGRYTSPHMESVTERISINGV
HHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCC
PADAEVFARAYDDVIPYAELVDSRHPERVTFFELLTAMAFSAFSDSPVDAGVVEVGMGGT
CCHHHHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCC
WDATNVVDAGVAVITPVSLDHPELGDTVEAVAAEKAGIIRPDALVVLAQQSLPAAEVLLR
CCCCHHHHCCEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHH
RSAEVGATVAREGLEFGVVGRRVAVGGQVLTLRGLGGTYEEVFLPLHGIHQAHNAAVALA
HHHHHHHHHHHCCCCCCCCCCEEEECCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
AVEAFLGGGQGQLDADAVLAGFAQADSPGRLEVVRRSPTIVLDGAHNVAGAQALAAAVDE
HHHHHHCCCCCCCCHHHHHHHHHCCCCCCCEEEEECCCEEEEECCCCHHHHHHHHHHHHH
AFGFDNLVGVVGVLGDKDAEGILAALEPVLSSVVVTQSTSPRAMGADDLAAIAVDVFDAD
HCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCC
RVEVAPRLDDALEAAVRLAEEEGELGGTGVLVTGSLVTVGEARHLLRR
CEEECCCHHHHHHHHHHHHHHCCCCCCCEEEEECCEEEHHHHHHHHCC
>Mature Secondary Structure 
PAPAAPSERASRCHNLGSVTDARTGRTPDDAAELARVEKALAGRFPHRMHPDLDRMRDL
CCCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
VDLLGHPERSFPSIHITGTNGKTSTARMIDALLRGFGLRPGRYTSPHMESVTERISINGV
HHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCC
PADAEVFARAYDDVIPYAELVDSRHPERVTFFELLTAMAFSAFSDSPVDAGVVEVGMGGT
CCHHHHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCC
WDATNVVDAGVAVITPVSLDHPELGDTVEAVAAEKAGIIRPDALVVLAQQSLPAAEVLLR
CCCCHHHHCCEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHH
RSAEVGATVAREGLEFGVVGRRVAVGGQVLTLRGLGGTYEEVFLPLHGIHQAHNAAVALA
HHHHHHHHHHHCCCCCCCCCCEEEECCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
AVEAFLGGGQGQLDADAVLAGFAQADSPGRLEVVRRSPTIVLDGAHNVAGAQALAAAVDE
HHHHHHCCCCCCCCHHHHHHHHHCCCCCCCEEEEECCCEEEEECCCCHHHHHHHHHHHHH
AFGFDNLVGVVGVLGDKDAEGILAALEPVLSSVVVTQSTSPRAMGADDLAAIAVDVFDAD
HCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCC
RVEVAPRLDDALEAAVRLAEEEGELGGTGVLVTGSLVTVGEARHLLRR
CEEECCCHHHHHHHHHHHHHHCCCCCCCEEEEECCEEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8419299; 9384377; 2553669 [H]