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The map label for this gene is thcD [H]

Identifier: 158313674

GI number: 158313674

Start: 2207440

End: 2208744

Strand: Direct

Name: thcD [H]

Synonym: Franean1_1838

Alternate gene names: 158313674

Gene position: 2207440-2208744 (Clockwise)

Preceding gene: 158313673

Following gene: 158313675

Centisome position: 24.58

GC content: 76.25

Gene sequence:

>1305_bases
ATGGATCGCGTCGTCGTCGTCGGTGGGGGGCTCGCCGGAGGGCGAGCGGTCGTCGAGCTGCGGGAGCAGGGCTTCGCGGG
CGCGATCACTCTGGTGGGCGCGGAGGAGCATCGGCCCTATGACCGGCCGCCGCTGTCGAAGGCGGTGCTGTCGGGGCGGA
CGGACGACACCACGCTGCCCTTCGACCTCGACGGCGTGGAGCTTCTGCTCGGCCGGCACGCGACCGGGCTGCGCCCGGGT
GTGCTGGAGACCGACGGCGGCGCCGTCGAGTTCGACGGGCTGGTGCTCGCGACCGGCGCCGCCCCGGTGCGGCTGCCCGG
CCCCGGCGAGCAGCGGGTGCTGCGCACCATCGACGACGCCCTCGCGCTGCGCGAGGCGCTGACCCCGGGTATCCGGCTGG
CCATCGTCGGCGCGGGCTGGATCGGCGCCGAGGTCGCCACGCACGCGGCGTCCGCCGGCGCGGCCGTGACCGTCGTCGAG
GCCGGCTCCGCGCCGCTGCTCGCGCTGGGTGCCGAGATCGGCGGGTACACGGTCCCCTGGTACGCCAAGGTGGGGATCGA
CCTGCGGCTCGGTGCCGCGGTCGACGAGATCCGGGCGGACGGGCTCGCGCTGGCCGGGGGCGAGTTCGTGCCTGCCGACG
TGGTGGTCGTGGGAGTCGGCGTCCGCCCGGACGTCGCCTGGCTGGAGTCGTCGGGTCTGCCGCTGGACCGCGGCGTCGTC
GTCGACGAATGGCTGTCGGCGGTGCCCGGCGCCAGGGTGGCCGACGCCGGCGGGCCGGGCACGGCCGACGGGGCCGGCGG
CTACCGGCCGGATTCGGTCGTCGCGGTGGGGGACTGCGCCGCGTGGTGGTCCCGGCGTTACGACCGCCGGTTGCGCGTCG
AGCACTGGGAATGCGCCCAGGAGTCGCCGCGGGTCGCTGTCGCGACCCTGCTGCGTTCGCTGGCCGGCGGTGACGGGCCG
GCGCTGGCCGGCGACCAGGCCGAAATCTACGATCCCGTCCCGTACTTCTGGTCGCAGCAGTTCGGGCGCATGATCCAGTT
CGCCGGGATGCCCGGCGCGGACAGCACGCTGGTGCTGCGGGGGGATCCGACCGCCGTCGCGCCGGAGGGCGCGAAGCGGG
CGCCGGGCTGGTCGGCGGGCTGGTTCGCCCCGGACGGCGAGCTGGTCGCTCTGGTGACCGTCGCCCGACCACTGGACATG
GTCGCCGGCCGGCGGATGCTGCTCGCCGGCGTGGCGCCGGACGTCGAGCGGTTCGCGGACCCCGCGGTCGCGCTGAAGGA
GCTCCAGCCCGCGCAGCCGGTGTGA

Upstream 100 bases:

>100_bases
GCACCGCCGCGGGCCGCCCGCCGTTGTGGGCAGCGCTACTCACGGGCGACGCGGAACAGATCGTGCAAGTAACCTCTCGG
CCCATGGATCGGGTGGGCTG

Downstream 100 bases:

>100_bases
GACGGGCGGCCGCCGGCCGCTCCGGCGGCCGGCGGTCTTTGTCGTGCCCGTAGTTGACTACTTTTCGTAACGATATTGTT
TACTTTTTGTCGTGGAGTGC

Product: FAD-dependent pyridine nucleotide-disulfide oxidoreductase

Products: Oxidized Ferredoxin; NADH; cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 434; Mature: 434

Protein sequence:

>434_residues
MDRVVVVGGGLAGGRAVVELREQGFAGAITLVGAEEHRPYDRPPLSKAVLSGRTDDTTLPFDLDGVELLLGRHATGLRPG
VLETDGGAVEFDGLVLATGAAPVRLPGPGEQRVLRTIDDALALREALTPGIRLAIVGAGWIGAEVATHAASAGAAVTVVE
AGSAPLLALGAEIGGYTVPWYAKVGIDLRLGAAVDEIRADGLALAGGEFVPADVVVVGVGVRPDVAWLESSGLPLDRGVV
VDEWLSAVPGARVADAGGPGTADGAGGYRPDSVVAVGDCAAWWSRRYDRRLRVEHWECAQESPRVAVATLLRSLAGGDGP
ALAGDQAEIYDPVPYFWSQQFGRMIQFAGMPGADSTLVLRGDPTAVAPEGAKRAPGWSAGWFAPDGELVALVTVARPLDM
VAGRRMLLAGVAPDVERFADPAVALKELQPAQPV

Sequences:

>Translated_434_residues
MDRVVVVGGGLAGGRAVVELREQGFAGAITLVGAEEHRPYDRPPLSKAVLSGRTDDTTLPFDLDGVELLLGRHATGLRPG
VLETDGGAVEFDGLVLATGAAPVRLPGPGEQRVLRTIDDALALREALTPGIRLAIVGAGWIGAEVATHAASAGAAVTVVE
AGSAPLLALGAEIGGYTVPWYAKVGIDLRLGAAVDEIRADGLALAGGEFVPADVVVVGVGVRPDVAWLESSGLPLDRGVV
VDEWLSAVPGARVADAGGPGTADGAGGYRPDSVVAVGDCAAWWSRRYDRRLRVEHWECAQESPRVAVATLLRSLAGGDGP
ALAGDQAEIYDPVPYFWSQQFGRMIQFAGMPGADSTLVLRGDPTAVAPEGAKRAPGWSAGWFAPDGELVALVTVARPLDM
VAGRRMLLAGVAPDVERFADPAVALKELQPAQPV
>Mature_434_residues
MDRVVVVGGGLAGGRAVVELREQGFAGAITLVGAEEHRPYDRPPLSKAVLSGRTDDTTLPFDLDGVELLLGRHATGLRPG
VLETDGGAVEFDGLVLATGAAPVRLPGPGEQRVLRTIDDALALREALTPGIRLAIVGAGWIGAEVATHAASAGAAVTVVE
AGSAPLLALGAEIGGYTVPWYAKVGIDLRLGAAVDEIRADGLALAGGEFVPADVVVVGVGVRPDVAWLESSGLPLDRGVV
VDEWLSAVPGARVADAGGPGTADGAGGYRPDSVVAVGDCAAWWSRRYDRRLRVEHWECAQESPRVAVATLLRSLAGGDGP
ALAGDQAEIYDPVPYFWSQQFGRMIQFAGMPGADSTLVLRGDPTAVAPEGAKRAPGWSAGWFAPDGELVALVTVARPLDM
VAGRRMLLAGVAPDVERFADPAVALKELQPAQPV

Specific function: The degradation of the thiocarbamate herbicide EPTC by cytochrome CYP116 (thcB) requires the participation of a flavoprotein, rhodocoxin reductase, and an iron-sulfur protein, rhodocoxin, to mediate the transfer of electrons from NADH to P450 for oxygen a

COG id: COG0446

COG function: function code R; Uncharacterized NAD(FAD)-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI65787454, Length=380, Percent_Identity=28.4210526315789, Blast_Score=128, Evalue=8e-30,
Organism=Homo sapiens, GI226437568, Length=380, Percent_Identity=28.4210526315789, Blast_Score=128, Evalue=9e-30,
Organism=Homo sapiens, GI21389617, Length=380, Percent_Identity=28.4210526315789, Blast_Score=128, Evalue=9e-30,
Organism=Escherichia coli, GI1788892, Length=448, Percent_Identity=30.3571428571429, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1789065, Length=262, Percent_Identity=28.6259541984733, Blast_Score=89, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17559934, Length=383, Percent_Identity=26.3707571801567, Blast_Score=98, Evalue=9e-21,
Organism=Drosophila melanogaster, GI281359715, Length=372, Percent_Identity=28.2258064516129, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI281359713, Length=372, Percent_Identity=28.2258064516129, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24639250, Length=372, Percent_Identity=28.2258064516129, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI18543267, Length=372, Percent_Identity=28.2258064516129, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24639252, Length=372, Percent_Identity=28.2258064516129, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24639257, Length=372, Percent_Identity=28.2258064516129, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24585130, Length=367, Percent_Identity=25.6130790190736, Blast_Score=105, Evalue=7e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR004099
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.18.1.3 [C]

Molecular weight: Translated: 44771; Mature: 44771

Theoretical pI: Translated: 4.46; Mature: 4.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRVVVVGGGLAGGRAVVELREQGFAGAITLVGAEEHRPYDRPPLSKAVLSGRTDDTTLP
CCEEEEEECCCCCCHHHHHHHHCCCCEEEEEEECHHCCCCCCCCHHHHHHCCCCCCCCCC
FDLDGVELLLGRHATGLRPGVLETDGGAVEFDGLVLATGAAPVRLPGPGEQRVLRTIDDA
CCCCCHHHHHCCCCCCCCCCEEECCCCEEEECCEEEECCCCCEECCCCCHHHHHHHHHHH
LALREALTPGIRLAIVGAGWIGAEVATHAASAGAAVTVVEAGSAPLLALGAEIGGYTVPW
HHHHHHCCCCEEEEEEECCCHHHHHHHHHHCCCCEEEEEECCCCCEEEEECCCCCEECCE
YAKVGIDLRLGAAVDEIRADGLALAGGEFVPADVVVVGVGVRPDVAWLESSGLPLDRGVV
EEEECEEEEECCHHHHHCCCCEEEECCCCCCCCEEEEECCCCCCHHEECCCCCCCCCCEE
VDEWLSAVPGARVADAGGPGTADGAGGYRPDSVVAVGDCAAWWSRRYDRRLRVEHWECAQ
HHHHHHHCCCCEEECCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHCCCCCCHHHHHHC
ESPRVAVATLLRSLAGGDGPALAGDQAEIYDPVPYFWSQQFGRMIQFAGMPGADSTLVLR
CCCCHHHHHHHHHHCCCCCCCCCCCCCHHCCCCCHHHHHHHHHHEEECCCCCCCCEEEEE
GDPTAVAPEGAKRAPGWSAGWFAPDGELVALVTVARPLDMVAGRRMLLAGVAPDVERFAD
CCCCEECCCCCCCCCCCCCCCCCCCCCEEEEEEEHHHHHHHHCCEEEEECCCCCHHHHCC
PAVALKELQPAQPV
HHHHHHHCCCCCCC
>Mature Secondary Structure
MDRVVVVGGGLAGGRAVVELREQGFAGAITLVGAEEHRPYDRPPLSKAVLSGRTDDTTLP
CCEEEEEECCCCCCHHHHHHHHCCCCEEEEEEECHHCCCCCCCCHHHHHHCCCCCCCCCC
FDLDGVELLLGRHATGLRPGVLETDGGAVEFDGLVLATGAAPVRLPGPGEQRVLRTIDDA
CCCCCHHHHHCCCCCCCCCCEEECCCCEEEECCEEEECCCCCEECCCCCHHHHHHHHHHH
LALREALTPGIRLAIVGAGWIGAEVATHAASAGAAVTVVEAGSAPLLALGAEIGGYTVPW
HHHHHHCCCCEEEEEEECCCHHHHHHHHHHCCCCEEEEEECCCCCEEEEECCCCCEECCE
YAKVGIDLRLGAAVDEIRADGLALAGGEFVPADVVVVGVGVRPDVAWLESSGLPLDRGVV
EEEECEEEEECCHHHHHCCCCEEEECCCCCCCCEEEEECCCCCCHHEECCCCCCCCCCEE
VDEWLSAVPGARVADAGGPGTADGAGGYRPDSVVAVGDCAAWWSRRYDRRLRVEHWECAQ
HHHHHHHCCCCEEECCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHCCCCCCHHHHHHC
ESPRVAVATLLRSLAGGDGPALAGDQAEIYDPVPYFWSQQFGRMIQFAGMPGADSTLVLR
CCCCHHHHHHHHHHCCCCCCCCCCCCCHHCCCCCHHHHHHHHHHEEECCCCCCCCEEEEE
GDPTAVAPEGAKRAPGWSAGWFAPDGELVALVTVARPLDMVAGRRMLLAGVAPDVERFAD
CCCCEECCCCCCCCCCCCCCCCCCCCCEEEEEEEHHHHHHHHCCEEEEECCCCCHHHHCC
PAVALKELQPAQPV
HHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Reduced Ferredoxin; NAD(+); 3-phenylpropionate; NADH; Proton; O2 [C]

Specific reaction: Reduced Ferredoxin + NAD(+) = Oxidized Ferredoxin + NADH. 3-phenylpropionate + NADH + Proton + O2 = cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7836301 [H]