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The map label for this gene is thiH [H]

Identifier: 158313673

GI number: 158313673

Start: 2206156

End: 2207322

Strand: Direct

Name: thiH [H]

Synonym: Franean1_1837

Alternate gene names: 158313673

Gene position: 2206156-2207322 (Clockwise)

Preceding gene: 158313672

Following gene: 158313674

Centisome position: 24.56

GC content: 75.15

Gene sequence:

>1167_bases
ATGGCCAGCCCGGCAGGGCTGTTCGCCCGCGAGCTCGCCGCGCTCGACATCCCGGCGCTCGCCCGTGTCTCGGTCGAGGC
CGACGAGGCGCGGGTCGACGCCGTCCTGCGCCGGGCCGTCGCTGCCGGGCGGCCCGACGCCGGCGGCCGGCTCGACCTCG
CCGACCTCGCCGTCCTGCTGTCGCCGGCCGCGACCGGGCGGCTGGAGGAGCTGGCGCAGGCGGCGCGGGAGACGACGCTG
CGCCGGTTCGGCCGGGCGGTGCGGCTGTTCGCCCCGCTGTACGTGTCGAACGCCTGCCTGTCGTCCTGCACCTACTGCGG
GTTCGCCAAGGGGCTGGAGGTGGCCCGGCGCACCCTGACGGTCGACGAAGCCGAGGCCGAGGCACGCCTGCTGGCCGACC
GCGGCTTCCGGCACATCCTGCTGGTCTCCGGGGAGCACCGCGTCGAGGTCTCCGCCGGGTACCTGGTGGACGTCGTCGAG
CGGCTGCGACCGTTCGTCCCCTCGATCTCGGTGGAGACCCAGACCTGGTCGGACGACACCTACAGCCGGCTGGTCGTGGC
CGGGCTCGACGGCGTCGTCCACTACCAGGAGACCTACGACCGGGAGCGCTACGCGCAGGTGCACGTGGCCGGGTGGAAGC
GCGACTACGACCGCCGGCTGTCCTCCTTCGAGCGGGCGGCCCGCGCCGGCGCCCGCCGTCTGGGCCTCGGCGTCCTGCTC
GGCCTGGCGCCGGACTGGCGGGCCGACGTCCTCGCGCTCGCCGCGCACGCCTCGTTCCTCGCCCGCCGCTTCTGGCGGAC
GGAGGTCTCGGTGGCGCTGCCGAGGATCAAGCCGAGCGCCAGTGGCTTCCCACCGACCGTCGTCGTCGGCGACGCCGAGT
TCGTCCAGGCGCACGCGGCGCTGCGGCTGTTCGAACCGGACGCGGCGATCTCGCTGTCGACCCGCGAGCCGGCGGCCCTG
CGTGACGGCCTGGTCCGCATCGCGGTGACCACGATGAGCGCCGGCTCGTCCACCGAGCCAGGTGGGTACGGGCGGCCCGG
GACGGCGCAGGAGCAGTTCTCCATCTCCGACGAGCGATCCCCGGCGGACGTCGCCGCGATGCTCGTCGGCGCCGGCTACG
AGCCTGTCTGGAAGGACGCGTTCCCGCTGGTCGACGCCGCCGGCTGA

Upstream 100 bases:

>100_bases
GGGCGGGCAGCGCCACCGTGGCCGAGGCGTCCTCTCCGCTCACCGGTTTCCTGGGCGCGGTACCCGGCGGCCTGCCCGGT
CTGCCCGGCGGGGGCGGCTG

Downstream 100 bases:

>100_bases
CCGGGCATCCTCCGCCGGCACCGCCGCGGGCCGCCCGCCGTTGTGGGCAGCGCTACTCACGGGCGACGCGGAACAGATCG
TGCAAGTAACCTCTCGGCCC

Product: thiamine biosynthesis protein ThiH

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: Dehydroglycine synthase; Tyrosine lyase [H]

Number of amino acids: Translated: 388; Mature: 387

Protein sequence:

>388_residues
MASPAGLFARELAALDIPALARVSVEADEARVDAVLRRAVAAGRPDAGGRLDLADLAVLLSPAATGRLEELAQAARETTL
RRFGRAVRLFAPLYVSNACLSSCTYCGFAKGLEVARRTLTVDEAEAEARLLADRGFRHILLVSGEHRVEVSAGYLVDVVE
RLRPFVPSISVETQTWSDDTYSRLVVAGLDGVVHYQETYDRERYAQVHVAGWKRDYDRRLSSFERAARAGARRLGLGVLL
GLAPDWRADVLALAAHASFLARRFWRTEVSVALPRIKPSASGFPPTVVVGDAEFVQAHAALRLFEPDAAISLSTREPAAL
RDGLVRIAVTTMSAGSSTEPGGYGRPGTAQEQFSISDERSPADVAAMLVGAGYEPVWKDAFPLVDAAG

Sequences:

>Translated_388_residues
MASPAGLFARELAALDIPALARVSVEADEARVDAVLRRAVAAGRPDAGGRLDLADLAVLLSPAATGRLEELAQAARETTL
RRFGRAVRLFAPLYVSNACLSSCTYCGFAKGLEVARRTLTVDEAEAEARLLADRGFRHILLVSGEHRVEVSAGYLVDVVE
RLRPFVPSISVETQTWSDDTYSRLVVAGLDGVVHYQETYDRERYAQVHVAGWKRDYDRRLSSFERAARAGARRLGLGVLL
GLAPDWRADVLALAAHASFLARRFWRTEVSVALPRIKPSASGFPPTVVVGDAEFVQAHAALRLFEPDAAISLSTREPAAL
RDGLVRIAVTTMSAGSSTEPGGYGRPGTAQEQFSISDERSPADVAAMLVGAGYEPVWKDAFPLVDAAG
>Mature_387_residues
ASPAGLFARELAALDIPALARVSVEADEARVDAVLRRAVAAGRPDAGGRLDLADLAVLLSPAATGRLEELAQAARETTLR
RFGRAVRLFAPLYVSNACLSSCTYCGFAKGLEVARRTLTVDEAEAEARLLADRGFRHILLVSGEHRVEVSAGYLVDVVER
LRPFVPSISVETQTWSDDTYSRLVVAGLDGVVHYQETYDRERYAQVHVAGWKRDYDRRLSSFERAARAGARRLGLGVLLG
LAPDWRADVLALAAHASFLARRFWRTEVSVALPRIKPSASGFPPTVVVGDAEFVQAHAALRLFEPDAAISLSTREPAALR
DGLVRIAVTTMSAGSSTEPGGYGRPGTAQEQFSISDERSPADVAAMLVGAGYEPVWKDAFPLVDAAG

Specific function: Catalyzes the radical-mediated cleavage of tyrosine to 2-iminoacetate and 4-cresol [H]

COG id: COG1060

COG function: function code HR; Thiamine biosynthesis enzyme ThiH and related uncharacterized enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. ThiH family [H]

Homologues:

Organism=Escherichia coli, GI1790423, Length=356, Percent_Identity=39.3258426966292, Blast_Score=274, Evalue=8e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR007197
- InterPro:   IPR012726 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 41769; Mature: 41638

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASPAGLFARELAALDIPALARVSVEADEARVDAVLRRAVAAGRPDAGGRLDLADLAVLL
CCCCHHHHHHHHHHCCCCHHHEEECCHHHHHHHHHHHHHHHCCCCCCCCEECHHHHHHHH
SPAATGRLEELAQAARETTLRRFGRAVRLFAPLYVSNACLSSCTYCGFAKGLEVARRTLT
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
VDEAEAEARLLADRGFRHILLVSGEHRVEVSAGYLVDVVERLRPFVPSISVETQTWSDDT
CCHHHHHHHHHHHCCCEEEEEECCCCEEEECCCHHHHHHHHHHHCCCCCEEEECCCCCCH
YSRLVVAGLDGVVHYQETYDRERYAQVHVAGWKRDYDRRLSSFERAARAGARRLGLGVLL
HHHEEEECCCCCEEHHHHHCHHHHEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
GLAPDWRADVLALAAHASFLARRFWRTEVSVALPRIKPSASGFPPTVVVGDAEFVQAHAA
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCEEEECCHHHHHHHHH
LRLFEPDAAISLSTREPAALRDGLVRIAVTTMSAGSSTEPGGYGRPGTAQEQFSISDERS
HEEECCCCEEEECCCCCHHHHCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCC
PADVAAMLVGAGYEPVWKDAFPLVDAAG
HHHHHHHHHCCCCCCHHHCCCCCCCCCC
>Mature Secondary Structure 
ASPAGLFARELAALDIPALARVSVEADEARVDAVLRRAVAAGRPDAGGRLDLADLAVLL
CCCHHHHHHHHHHCCCCHHHEEECCHHHHHHHHHHHHHHHCCCCCCCCEECHHHHHHHH
SPAATGRLEELAQAARETTLRRFGRAVRLFAPLYVSNACLSSCTYCGFAKGLEVARRTLT
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
VDEAEAEARLLADRGFRHILLVSGEHRVEVSAGYLVDVVERLRPFVPSISVETQTWSDDT
CCHHHHHHHHHHHCCCEEEEEECCCCEEEECCCHHHHHHHHHHHCCCCCEEEECCCCCCH
YSRLVVAGLDGVVHYQETYDRERYAQVHVAGWKRDYDRRLSSFERAARAGARRLGLGVLL
HHHEEEECCCCCEEHHHHHCHHHHEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
GLAPDWRADVLALAAHASFLARRFWRTEVSVALPRIKPSASGFPPTVVVGDAEFVQAHAA
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCEEEECCHHHHHHHHH
LRLFEPDAAISLSTREPAALRDGLVRIAVTTMSAGSSTEPGGYGRPGTAQEQFSISDERS
HEEECCCCEEEECCCCCHHHHCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCC
PADVAAMLVGAGYEPVWKDAFPLVDAAG
HHHHHHHHHCCCCCCHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8432721; 8265357; 9278503; 10082377 [H]