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The map label for this gene is yfkN [H]

Identifier: 157963488

GI number: 157963488

Start: 4475976

End: 4478126

Strand: Direct

Name: yfkN [H]

Synonym: Spea_3676

Alternate gene names: 157963488

Gene position: 4475976-4478126 (Clockwise)

Preceding gene: 157963485

Following gene: 157963490

Centisome position: 86.5

GC content: 42.07

Gene sequence:

>2151_bases
ATGAAATTAAGTACTAAGAAGCTTCTGCTTGTTTCAAGCATCGCTGCTGCTTTAGTGGGTTGTAACAGCTCATCAGACGA
TGATAATAATGTTAGTGACAACACCATCACTGTACGTTTGATGGAAACAACAGACATCCACACCAACGTAATGCCGTACA
ACTACTTTGTCAGCGAAGAAGTCAACGAAACTACGTTACCTGAATGGGGCTTAGCGCGTACTTCTTTGGTTATCGAAGCT
GCACGTAATGAAGTTGAAAATAGCATGCTATTTGATAACGGTGACTTAATCCAAGGTAGCCCAATGGGCGACTATATGGC
GTCACTGGGTACTGATCACGTTAAAGAAAATACCCACCCTGTTTATAAAGCAATGAACGAATTGCAATACGACGCAGCTA
ACTTGGGTAACCATGAGTTTAACTACGGTTTAGATTTCCTAGACGAGTCTCTTAAAGGTTCAGACTTCCCTTATGTGAGC
GCCAACGTATGGAAAGTTGATAACTCGCTAGAAAAAGTAAGTAATGCCGCAGAAGAGTGTGAAGTTCGTATTACTGAAGA
GTTTTATGAGGAAGCTGAACATAAGTATCAGCCATACGTGATCTTAGATCGTGAATTTAAGAGCAGCAACGGTGAGTCTT
ACTTAGTTAAAGTCGGTGTTATCGGCTTTACCCCACCTAATATTATGGGTTGGGATGCATCATTACTAAAATGTAATGTC
ATGGTGTCTGACATCAAGAAAACTGCTGAACATTATGTTCCAAAGATGAAAGCTGAAGGCGCAGACATTATTGTTGCTAT
CCCTCACTCAGGTCTAAATGACAATGACAATGAGTTTGCTGAAAACGCAACACTGCACCTTGCCTATGTTGACGACATTG
ATGCCATCATGTTTGGCCACGATCACCGTGAATTCCCTAATACAACCGGAGAATACGGCGAAATCGAAGGCGTTGATGCC
GAGCTTGGATTAATTAATGGTGTTCCAGCTGTTATGCCAGGTTTCTGGGGCGCTAAGCTAGGTGTTATTGACTTAACGCT
AACAACTAAAGATAACGGCGAAAGCTGGAGCGTTGACCATACTAAGTCAACATCAAAACTACGCTCACTTATTCCTAATA
GTTCAAATCGTATTATCGAAGATTTAGTCGCTGATGAGCACCAAGGCACGATTGATTACATGTCTACATCTATTGCTTCA
ATCGATGTGAACATGAACAGCTTCTTCCCACAAGTGGTACCTGATTTATCTATTCAAGTGGTTAACGAAGCGCAACTGCA
CCAGTTAATGAAGTGGAAAGAAGCTGGTGAATTTGCCAATGCTGCTGACGACGCTATCTTCTTGTCAGTTTCTGCACCAT
TCAAGTCTGGCCGTAACGGTCCACAAGACTTTACCAACATCCAAGAAGGCGAGCTAACTAACGCATCTGTTGCTGATATC
TATGTATTTGATAACAATACGCCAGCAGTGTTAAAGATGACTGGTTCAGATATGAAGAACTGGATTGAGTGGGTTAACTC
AAACGCATACAACAGCCTACCTTTAGCGGCTGGTGAGCGCTTCCTAAATGAATCATTCCCTGGCTATAACTTTGACGTAT
TCTTCGGTGGCTTCAGCCAAGCTGGTGAAGGCTTACTTAAGTACACAGTTAACGTTGAGAATGAGTCAAAGTACATCGAC
ATTAACGGTTTTGAGTACACAGAGACTGAAAATAAGCAGATCGACAGCCTAACTTATAACGGCGTTGAAATTGCTGATGA
CGCAGAAATCTATGTTATTACTAACAACTACCGTGCGTCTAACGTAAAAATGCCTGGTGTTGATAAAGCCGTTCTTGTTA
AAGAAGAAGCGGCATTTAACAACCGTGAGTTAGTGCAATACTACCTAGAAGACTCGCTAGCTGATAATCAAGGTGACGCG
TCATTAGCATTCCCGAATGCCGAAGTGTTCCAACTTATAGCACCAAACACCGATAGCGTTTGGTTTGCATCAGCAACACT
TGATGAAGCATTCCGCTGTGTTGACAGTGGTATCACAGGTCTTTCTACTACAAAAGAAGAAGAAACTAAAGCGGGTACTG
AAGGCTTTACTAAGTTCCACTTTAACTTTGACTACAACGGTGAGTTTAACTGTGCGGCTAAAGCTAAGTAA

Upstream 100 bases:

>100_bases
GTTTAGTTCGCGTATCCAACATCACTTAACCTTCCCAATTTAATAATTAAGTGTTGCTATCAATTTTAATAAAAAATAAT
AAATGGTTTTTGGAGTAAAT

Downstream 100 bases:

>100_bases
CTAACACTAAGTTACTAACGCTAAGTAACTGGCACCAAGCGACTAACGCTAAGCAACTTAAGCCGCTTTAAACCGAGTTA
GATTAAAATCAAAAATCCCT

Product: metallophosphoesterase

Products: NA

Alternate protein names: 2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase; 5'-nucleotidase [H]

Number of amino acids: Translated: 716; Mature: 716

Protein sequence:

>716_residues
MKLSTKKLLLVSSIAAALVGCNSSSDDDNNVSDNTITVRLMETTDIHTNVMPYNYFVSEEVNETTLPEWGLARTSLVIEA
ARNEVENSMLFDNGDLIQGSPMGDYMASLGTDHVKENTHPVYKAMNELQYDAANLGNHEFNYGLDFLDESLKGSDFPYVS
ANVWKVDNSLEKVSNAAEECEVRITEEFYEEAEHKYQPYVILDREFKSSNGESYLVKVGVIGFTPPNIMGWDASLLKCNV
MVSDIKKTAEHYVPKMKAEGADIIVAIPHSGLNDNDNEFAENATLHLAYVDDIDAIMFGHDHREFPNTTGEYGEIEGVDA
ELGLINGVPAVMPGFWGAKLGVIDLTLTTKDNGESWSVDHTKSTSKLRSLIPNSSNRIIEDLVADEHQGTIDYMSTSIAS
IDVNMNSFFPQVVPDLSIQVVNEAQLHQLMKWKEAGEFANAADDAIFLSVSAPFKSGRNGPQDFTNIQEGELTNASVADI
YVFDNNTPAVLKMTGSDMKNWIEWVNSNAYNSLPLAAGERFLNESFPGYNFDVFFGGFSQAGEGLLKYTVNVENESKYID
INGFEYTETENKQIDSLTYNGVEIADDAEIYVITNNYRASNVKMPGVDKAVLVKEEAAFNNRELVQYYLEDSLADNQGDA
SLAFPNAEVFQLIAPNTDSVWFASATLDEAFRCVDSGITGLSTTKEEETKAGTEGFTKFHFNFDYNGEFNCAAKAK

Sequences:

>Translated_716_residues
MKLSTKKLLLVSSIAAALVGCNSSSDDDNNVSDNTITVRLMETTDIHTNVMPYNYFVSEEVNETTLPEWGLARTSLVIEA
ARNEVENSMLFDNGDLIQGSPMGDYMASLGTDHVKENTHPVYKAMNELQYDAANLGNHEFNYGLDFLDESLKGSDFPYVS
ANVWKVDNSLEKVSNAAEECEVRITEEFYEEAEHKYQPYVILDREFKSSNGESYLVKVGVIGFTPPNIMGWDASLLKCNV
MVSDIKKTAEHYVPKMKAEGADIIVAIPHSGLNDNDNEFAENATLHLAYVDDIDAIMFGHDHREFPNTTGEYGEIEGVDA
ELGLINGVPAVMPGFWGAKLGVIDLTLTTKDNGESWSVDHTKSTSKLRSLIPNSSNRIIEDLVADEHQGTIDYMSTSIAS
IDVNMNSFFPQVVPDLSIQVVNEAQLHQLMKWKEAGEFANAADDAIFLSVSAPFKSGRNGPQDFTNIQEGELTNASVADI
YVFDNNTPAVLKMTGSDMKNWIEWVNSNAYNSLPLAAGERFLNESFPGYNFDVFFGGFSQAGEGLLKYTVNVENESKYID
INGFEYTETENKQIDSLTYNGVEIADDAEIYVITNNYRASNVKMPGVDKAVLVKEEAAFNNRELVQYYLEDSLADNQGDA
SLAFPNAEVFQLIAPNTDSVWFASATLDEAFRCVDSGITGLSTTKEEETKAGTEGFTKFHFNFDYNGEFNCAAKAK
>Mature_716_residues
MKLSTKKLLLVSSIAAALVGCNSSSDDDNNVSDNTITVRLMETTDIHTNVMPYNYFVSEEVNETTLPEWGLARTSLVIEA
ARNEVENSMLFDNGDLIQGSPMGDYMASLGTDHVKENTHPVYKAMNELQYDAANLGNHEFNYGLDFLDESLKGSDFPYVS
ANVWKVDNSLEKVSNAAEECEVRITEEFYEEAEHKYQPYVILDREFKSSNGESYLVKVGVIGFTPPNIMGWDASLLKCNV
MVSDIKKTAEHYVPKMKAEGADIIVAIPHSGLNDNDNEFAENATLHLAYVDDIDAIMFGHDHREFPNTTGEYGEIEGVDA
ELGLINGVPAVMPGFWGAKLGVIDLTLTTKDNGESWSVDHTKSTSKLRSLIPNSSNRIIEDLVADEHQGTIDYMSTSIAS
IDVNMNSFFPQVVPDLSIQVVNEAQLHQLMKWKEAGEFANAADDAIFLSVSAPFKSGRNGPQDFTNIQEGELTNASVADI
YVFDNNTPAVLKMTGSDMKNWIEWVNSNAYNSLPLAAGERFLNESFPGYNFDVFFGGFSQAGEGLLKYTVNVENESKYID
INGFEYTETENKQIDSLTYNGVEIADDAEIYVITNNYRASNVKMPGVDKAVLVKEEAAFNNRELVQYYLEDSLADNQGDA
SLAFPNAEVFQLIAPNTDSVWFASATLDEAFRCVDSGITGLSTTKEEETKAGTEGFTKFHFNFDYNGEFNCAAKAK

Specific function: Catalyzes the release of inorganic phosphate from 2',3'- cyclic nucleotides through consecutive 2',3'-phosphodiesterase and 3'- (or 2') nucleotidase activities. Also possesses a 5'- nucleotidase activity. Does not catalyze the release of inorganic phospha

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Secreted, cell wall; Peptidoglycan-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Escherichia coli, GI1790658, Length=591, Percent_Identity=37.5634517766497, Blast_Score=356, Evalue=3e-99,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR019931
- InterPro:   IPR004843
- InterPro:   IPR001899 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.1.3.6; =3.1.4.16; =3.1.3.5 [H]

Molecular weight: Translated: 79230; Mature: 79230

Theoretical pI: Translated: 4.10; Mature: 4.10

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00786 5_NUCLEOTIDASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLSTKKLLLVSSIAAALVGCNSSSDDDNNVSDNTITVRLMETTDIHTNVMPYNYFVSEE
CCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCEEEEECCC
VNETTLPEWGLARTSLVIEAARNEVENSMLFDNGDLIQGSPMGDYMASLGTDHVKENTHP
CCCCCCCCCCCHHHHHHHHHHHHHHHHCEEECCCCEEECCCCHHHHHHCCCHHHCCCCCH
VYKAMNELQYDAANLGNHEFNYGLDFLDESLKGSDFPYVSANVWKVDNSLEKVSNAAEEC
HHHHHHHHHCCHHHCCCCCCCCCHHHHHHHCCCCCCCEEEEEEEECCHHHHHHHCHHHHH
EVRITEEFYEEAEHKYQPYVILDREFKSSNGESYLVKVGVIGFTPPNIMGWDASLLKCNV
EEEEHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEEEEEEEECCCCCCCCCCCHHEEEHH
MVSDIKKTAEHYVPKMKAEGADIIVAIPHSGLNDNDNEFAENATLHLAYVDDIDAIMFGH
HHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCHHHCCCEEEEEEECCCCEEEECC
DHREFPNTTGEYGEIEGVDAELGLINGVPAVMPGFWGAKLGVIDLTLTTKDNGESWSVDH
CCCCCCCCCCCCCCCCCCCCCCCEECCCHHHCCCCCCCEEEEEEEEEEECCCCCEECCCC
TKSTSKLRSLIPNSSNRIIEDLVADEHQGTIDYMSTSIASIDVNMNSFFPQVVPDLSIQV
CCHHHHHHHHCCCCCHHHHHHHHCCCCCCCHHHHHCEEEEEEECHHHHCHHHCCCCEEEE
VNEAQLHQLMKWKEAGEFANAADDAIFLSVSAPFKSGRNGPQDFTNIQEGELTNASVADI
ECHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCHHCCCCCCHHHCCCCCCCCCCCEEEEE
YVFDNNTPAVLKMTGSDMKNWIEWVNSNAYNSLPLAAGERFLNESFPGYNFDVFFGGFSQ
EEEECCCCEEEEECCHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCEEEEECCHHH
AGEGLLKYTVNVENESKYIDINGFEYTETENKQIDSLTYNGVEIADDAEIYVITNNYRAS
HCCCEEEEEEEECCCCCEEECCCEEECCCCCCEECEEEECCEEECCCCEEEEEECCCEEC
NVKMPGVDKAVLVKEEAAFNNRELVQYYLEDSLADNQGDASLAFPNAEVFQLIAPNTDSV
CEECCCCCCEEEEEHHHCCCCHHHHHHHHHHHCCCCCCCCEEECCCCCEEEEECCCCCCE
WFASATLDEAFRCVDSGITGLSTTKEEETKAGTEGFTKFHFNFDYNGEFNCAAKAK
EEEECCHHHHHHHHHCCCCCCCCCCHHHHHCCCCCCEEEEEEECCCCCEEEEECCC
>Mature Secondary Structure
MKLSTKKLLLVSSIAAALVGCNSSSDDDNNVSDNTITVRLMETTDIHTNVMPYNYFVSEE
CCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCEEEEECCC
VNETTLPEWGLARTSLVIEAARNEVENSMLFDNGDLIQGSPMGDYMASLGTDHVKENTHP
CCCCCCCCCCCHHHHHHHHHHHHHHHHCEEECCCCEEECCCCHHHHHHCCCHHHCCCCCH
VYKAMNELQYDAANLGNHEFNYGLDFLDESLKGSDFPYVSANVWKVDNSLEKVSNAAEEC
HHHHHHHHHCCHHHCCCCCCCCCHHHHHHHCCCCCCCEEEEEEEECCHHHHHHHCHHHHH
EVRITEEFYEEAEHKYQPYVILDREFKSSNGESYLVKVGVIGFTPPNIMGWDASLLKCNV
EEEEHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEEEEEEEECCCCCCCCCCCHHEEEHH
MVSDIKKTAEHYVPKMKAEGADIIVAIPHSGLNDNDNEFAENATLHLAYVDDIDAIMFGH
HHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCHHHCCCEEEEEEECCCCEEEECC
DHREFPNTTGEYGEIEGVDAELGLINGVPAVMPGFWGAKLGVIDLTLTTKDNGESWSVDH
CCCCCCCCCCCCCCCCCCCCCCCEECCCHHHCCCCCCCEEEEEEEEEEECCCCCEECCCC
TKSTSKLRSLIPNSSNRIIEDLVADEHQGTIDYMSTSIASIDVNMNSFFPQVVPDLSIQV
CCHHHHHHHHCCCCCHHHHHHHHCCCCCCCHHHHHCEEEEEEECHHHHCHHHCCCCEEEE
VNEAQLHQLMKWKEAGEFANAADDAIFLSVSAPFKSGRNGPQDFTNIQEGELTNASVADI
ECHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCHHCCCCCCHHHCCCCCCCCCCCEEEEE
YVFDNNTPAVLKMTGSDMKNWIEWVNSNAYNSLPLAAGERFLNESFPGYNFDVFFGGFSQ
EEEECCCCEEEEECCHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCEEEEECCHHH
AGEGLLKYTVNVENESKYIDINGFEYTETENKQIDSLTYNGVEIADDAEIYVITNNYRAS
HCCCEEEEEEEECCCCCEEECCCEEECCCCCCEECEEEECCEEECCCCEEEEEECCCEEC
NVKMPGVDKAVLVKEEAAFNNRELVQYYLEDSLADNQGDASLAFPNAEVFQLIAPNTDSV
CEECCCCCCEEEEEHHHCCCCHHHHHHHHHHHCCCCCCCCEEECCCCCEEEEECCCCCCE
WFASATLDEAFRCVDSGITGLSTTKEEETKAGTEGFTKFHFNFDYNGEFNCAAKAK
EEEECCHHHHHHHHHCCCCCCCCCCHHHHHCCCCCCEEEEEEECCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]