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The map label for this gene is phoQ [H]

Identifier: 157963326

GI number: 157963326

Start: 4290938

End: 4292263

Strand: Direct

Name: phoQ [H]

Synonym: Spea_3512

Alternate gene names: 157963326

Gene position: 4290938-4292263 (Clockwise)

Preceding gene: 157963325

Following gene: 157963362

Centisome position: 82.92

GC content: 46.98

Gene sequence:

>1326_bases
ATGATATCAATCCGCACCAAACTAAGCCTGTGGTTAACAGGCTTAGTGGTGATCTCCACTGCGGTGGGGATCATCTTTTT
CGAGTCTATGCTACGCCAAGCTTTCCACGACTCGATCATCGCCAGATTACAAGAAGATCTGCAGCAAGTCTTGCTCGCCA
CTCACCTAGAAGATAGCACCTTTAGCATAGATGAAAGCCAACTTTCCAGCTTCTATCGTCCTGTTTATTCAGGGCGTTAC
TATCAACTAGACCTACCTGAACAAGAATTAAGATCGCGCTCACTATGGGATCAACGCCTCGAGGTGGTGGATCTAGAGAA
AGGTGAAACCCGAGCTTGGCAGACTAAAGGGCCGCAAAACCACGATATTCAGCTGCTCTCTATCGGCCTGAGATCTGATA
CGTCTGGCCTTAACGCCACTCTTACGGTTGCGCAAGATCTGAGTATCGGACGCAAAGTTTTCTCCGAGGTCTATGGCACT
AAACTCATCGTAAATCTGGCCATGTTACTGGCGATGATCACAGGTATATTCTTAGTTCTACGCCAATCGTTTAAACCTGT
TCATCAGATGAAGGACACCTTGGCTAAATTGCGAGAAGGCGAAATATCCTCTTTTGACCTCAACTCTATTCCCTTAGAGC
TGCAGCCTTTAGCCAAAAACTACAATGAGCTACTCGCCTACTCCAGCAAGCAGATTGAACGCAGCCGCAATAATTTGGGT
AACTTAAGCCATGGCCTAAAGACACCGTTAGCTGTGATGCAGCAACAGGTAGAAGCTCTGTATTTAAAAGAGCCCGAATC
CGCCGAGGCGCTACAAAAACAACTCAATTTGGTGCATAAGATGATTGAGCGAAAACTGGCTGCGGCGCGGATCACCGGCG
ACATGCTACCTGCAGCGCAGATGCAACTGCCAAAAGATATCGAAGACTTAGCCCAAACCTTAGACAAGGTTCATCGCACC
AAATCGATTCACTGTCGCTTCGATATTCCAAGCGCCATCACTCGGCTCCCTATGCATCGTGAAGACGGCATGGAGCTATT
GGGTAACCTACTCGATAACGCTTATAAGTGGGCCAGTTCAGAAATTCGAGTCTGCATTAGCAACCATGAACAGCAGTTAA
AATTGACCATAGAAGATGATGGTCCAGGGGTTGAAACCGAGCAATTAGCCCAACTAACCCACAGAGGCAAACGTCTCGAT
GAAGCCACCATGGGCCATGGCTTAGGTTTGTCGATCGTTAAAGATATTGCCGAACAGTACAAGATTGAACTTAAGTTTGA
GCACAGTCAGAGTCTACCAGGTCTAAAAGTGACGTTATTGTTCTAA

Upstream 100 bases:

>100_bases
ATGTGGTTGAAGTTTACGTGACCCACTTACGTAAGAAGCTTGGCAAAACCGCCATCGAAACTCGTCGTGGCCAAGGGTAT
ATCTTTCACGGCCTAACCCA

Downstream 100 bases:

>100_bases
TCTTTATCCATAATGAGCAGCCAATAAAAAAACCGGACTCTGTCCGGCTTTTTTGTCTCTATCCGTAGATACTAGCCATT
GACGTTAGTACTTTCGAAGA

Product: histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 441; Mature: 441

Protein sequence:

>441_residues
MISIRTKLSLWLTGLVVISTAVGIIFFESMLRQAFHDSIIARLQEDLQQVLLATHLEDSTFSIDESQLSSFYRPVYSGRY
YQLDLPEQELRSRSLWDQRLEVVDLEKGETRAWQTKGPQNHDIQLLSIGLRSDTSGLNATLTVAQDLSIGRKVFSEVYGT
KLIVNLAMLLAMITGIFLVLRQSFKPVHQMKDTLAKLREGEISSFDLNSIPLELQPLAKNYNELLAYSSKQIERSRNNLG
NLSHGLKTPLAVMQQQVEALYLKEPESAEALQKQLNLVHKMIERKLAAARITGDMLPAAQMQLPKDIEDLAQTLDKVHRT
KSIHCRFDIPSAITRLPMHREDGMELLGNLLDNAYKWASSEIRVCISNHEQQLKLTIEDDGPGVETEQLAQLTHRGKRLD
EATMGHGLGLSIVKDIAEQYKIELKFEHSQSLPGLKVTLLF

Sequences:

>Translated_441_residues
MISIRTKLSLWLTGLVVISTAVGIIFFESMLRQAFHDSIIARLQEDLQQVLLATHLEDSTFSIDESQLSSFYRPVYSGRY
YQLDLPEQELRSRSLWDQRLEVVDLEKGETRAWQTKGPQNHDIQLLSIGLRSDTSGLNATLTVAQDLSIGRKVFSEVYGT
KLIVNLAMLLAMITGIFLVLRQSFKPVHQMKDTLAKLREGEISSFDLNSIPLELQPLAKNYNELLAYSSKQIERSRNNLG
NLSHGLKTPLAVMQQQVEALYLKEPESAEALQKQLNLVHKMIERKLAAARITGDMLPAAQMQLPKDIEDLAQTLDKVHRT
KSIHCRFDIPSAITRLPMHREDGMELLGNLLDNAYKWASSEIRVCISNHEQQLKLTIEDDGPGVETEQLAQLTHRGKRLD
EATMGHGLGLSIVKDIAEQYKIELKFEHSQSLPGLKVTLLF
>Mature_441_residues
MISIRTKLSLWLTGLVVISTAVGIIFFESMLRQAFHDSIIARLQEDLQQVLLATHLEDSTFSIDESQLSSFYRPVYSGRY
YQLDLPEQELRSRSLWDQRLEVVDLEKGETRAWQTKGPQNHDIQLLSIGLRSDTSGLNATLTVAQDLSIGRKVFSEVYGT
KLIVNLAMLLAMITGIFLVLRQSFKPVHQMKDTLAKLREGEISSFDLNSIPLELQPLAKNYNELLAYSSKQIERSRNNLG
NLSHGLKTPLAVMQQQVEALYLKEPESAEALQKQLNLVHKMIERKLAAARITGDMLPAAQMQLPKDIEDLAQTLDKVHRT
KSIHCRFDIPSAITRLPMHREDGMELLGNLLDNAYKWASSEIRVCISNHEQQLKLTIEDDGPGVETEQLAQLTHRGKRLD
EATMGHGLGLSIVKDIAEQYKIELKFEHSQSLPGLKVTLLF

Specific function: Member of the two-component regulatory system phoQ/phoP involved in virulence, adaptation to low Mg(2+) environments and the control of acid resistance genes. In presence of low periplasmic Mg(2+) concentrations, phoQ functions as a membrane- associated p

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787374, Length=306, Percent_Identity=26.1437908496732, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI1788393, Length=270, Percent_Identity=26.6666666666667, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1789808, Length=273, Percent_Identity=25.6410256410256, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1789403, Length=303, Percent_Identity=23.4323432343234, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1790346, Length=269, Percent_Identity=24.907063197026, Blast_Score=69, Evalue=8e-13,
Organism=Escherichia coli, GI1790551, Length=286, Percent_Identity=21.6783216783217, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1787894, Length=276, Percent_Identity=26.4492753623188, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1786600, Length=212, Percent_Identity=27.8301886792453, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR015014
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF08918 PhoQ_Sensor [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 49877; Mature: 49877

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISIRTKLSLWLTGLVVISTAVGIIFFESMLRQAFHDSIIARLQEDLQQVLLATHLEDST
CCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
FSIDESQLSSFYRPVYSGRYYQLDLPEQELRSRSLWDQRLEVVDLEKGETRAWQTKGPQN
CCCCHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCC
HDIQLLSIGLRSDTSGLNATLTVAQDLSIGRKVFSEVYGTKLIVNLAMLLAMITGIFLVL
CCEEEEEEECCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RQSFKPVHQMKDTLAKLREGEISSFDLNSIPLELQPLAKNYNELLAYSSKQIERSRNNLG
HHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEHHHCCHHHHHHHHHHHHHHHHHHCC
NLSHGLKTPLAVMQQQVEALYLKEPESAEALQKQLNLVHKMIERKLAAARITGDMLPAAQ
CHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
MQLPKDIEDLAQTLDKVHRTKSIHCRFDIPSAITRLPMHREDGMELLGNLLDNAYKWASS
HCCCCCHHHHHHHHHHHHCCCCEEEEECCHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCC
EIRVCISNHEQQLKLTIEDDGPGVETEQLAQLTHRGKRLDEATMGHGLGLSIVKDIAEQY
HHHHHCCCCCEEEEEEEECCCCCCCHHHHHHHHHHCCCCCHHHHCCCCCHHHHHHHHHHH
KIELKFEHSQSLPGLKVTLLF
EEEEEEECCCCCCCEEEEEEC
>Mature Secondary Structure
MISIRTKLSLWLTGLVVISTAVGIIFFESMLRQAFHDSIIARLQEDLQQVLLATHLEDST
CCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
FSIDESQLSSFYRPVYSGRYYQLDLPEQELRSRSLWDQRLEVVDLEKGETRAWQTKGPQN
CCCCHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCC
HDIQLLSIGLRSDTSGLNATLTVAQDLSIGRKVFSEVYGTKLIVNLAMLLAMITGIFLVL
CCEEEEEEECCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RQSFKPVHQMKDTLAKLREGEISSFDLNSIPLELQPLAKNYNELLAYSSKQIERSRNNLG
HHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEHHHCCHHHHHHHHHHHHHHHHHHCC
NLSHGLKTPLAVMQQQVEALYLKEPESAEALQKQLNLVHKMIERKLAAARITGDMLPAAQ
CHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
MQLPKDIEDLAQTLDKVHRTKSIHCRFDIPSAITRLPMHREDGMELLGNLLDNAYKWASS
HCCCCCHHHHHHHHHHHHCCCCEEEEECCHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCC
EIRVCISNHEQQLKLTIEDDGPGVETEQLAQLTHRGKRLDEATMGHGLGLSIVKDIAEQY
HHHHHCCCCCEEEEEEEECCCCCCCHHHHHHHHHHCCCCCHHHHCCCCCHHHHHHHHHHH
KIELKFEHSQSLPGLKVTLLF
EEEEEEECCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]