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The map label for this gene is blaI [H]

Identifier: 157963025

GI number: 157963025

Start: 3933667

End: 3934044

Strand: Direct

Name: blaI [H]

Synonym: Spea_3208

Alternate gene names: 157963025

Gene position: 3933667-3934044 (Clockwise)

Preceding gene: 157963020

Following gene: 157963026

Centisome position: 76.02

GC content: 46.56

Gene sequence:

>378_bases
ATGAAAGAGATCAGTAATGCAGAGTTGTGCGTACTCAATGTCCTGTGGCAATCCTCACCTATCAGTGCCAGTGATGTCAT
AGGTGAACTTTCTCGCACCCATGACTGGCACCAGAAAACCGTAAAAACATTACTCAACCGCCTAGTAAAGAAGCAAGCCA
TTGGCTTTGAAAAACAGGGGCGCTCCTACCTTTATAGTCCGCTAATCAGGCAGAGTGAGTATCAACTCAAGGAGAGTGAG
TCTTTTATCGAACGGTTATTTTCAGGACGAATCGCGCCCTTGGTCGCAGGATTTGCTAAGCAGAATAAGCTCAGCGCGGA
GGATGTCGACGAGCTACAACAGCTGATAGACACGTGGAAAAAGGATAACAAGTCATGA

Upstream 100 bases:

>100_bases
ACGCCTAACCTAGATTAAAAAACACACTAAAAATCACTTGCACAAACTGATTACATTTGTAATCTTAATTGAAATTACAA
ACGTAATCGAAGAGAAAGCA

Downstream 100 bases:

>100_bases
TGCTATGGATGGCGCAGCAGACCCTTTTACTCTCATTAGTATGTGGCGGCTTACTCTTGAGTCACCGTTTATTGCAGCAA
CACCTTGGCGCCCACAGAAC

Product: CopY family transcriptional regulator

Products: NA

Alternate protein names: Beta-lactamase repressor protein; Regulatory protein BlaI [H]

Number of amino acids: Translated: 125; Mature: 125

Protein sequence:

>125_residues
MKEISNAELCVLNVLWQSSPISASDVIGELSRTHDWHQKTVKTLLNRLVKKQAIGFEKQGRSYLYSPLIRQSEYQLKESE
SFIERLFSGRIAPLVAGFAKQNKLSAEDVDELQQLIDTWKKDNKS

Sequences:

>Translated_125_residues
MKEISNAELCVLNVLWQSSPISASDVIGELSRTHDWHQKTVKTLLNRLVKKQAIGFEKQGRSYLYSPLIRQSEYQLKESE
SFIERLFSGRIAPLVAGFAKQNKLSAEDVDELQQLIDTWKKDNKS
>Mature_125_residues
MKEISNAELCVLNVLWQSSPISASDVIGELSRTHDWHQKTVKTLLNRLVKKQAIGFEKQGRSYLYSPLIRQSEYQLKESE
SFIERLFSGRIAPLVAGFAKQNKLSAEDVDELQQLIDTWKKDNKS

Specific function: Transcriptional repressor that constitutively blocks expression of beta-lactamase. Regulates genes involved in antibiotic resistance. Binds DNA as a dimer [H]

COG id: COG3682

COG function: function code K; Predicted transcriptional regulator

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the BlaI transcriptional regulatory family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005650
- InterPro:   IPR011991 [H]

Pfam domain/function: PF03965 Pencillinase_R [H]

EC number: NA

Molecular weight: Translated: 14339; Mature: 14339

Theoretical pI: Translated: 9.07; Mature: 9.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEISNAELCVLNVLWQSSPISASDVIGELSRTHDWHQKTVKTLLNRLVKKQAIGFEKQG
CCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCC
RSYLYSPLIRQSEYQLKESESFIERLFSGRIAPLVAGFAKQNKLSAEDVDELQQLIDTWK
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
KDNKS
CCCCC
>Mature Secondary Structure
MKEISNAELCVLNVLWQSSPISASDVIGELSRTHDWHQKTVKTLLNRLVKKQAIGFEKQG
CCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCC
RSYLYSPLIRQSEYQLKESESFIERLFSGRIAPLVAGFAKQNKLSAEDVDELQQLIDTWK
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
KDNKS
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3096969; 3305074; 3260234; 3040663; 3498148 [H]