Click here to switch to the map view.

The map label for this gene is metR [H]

Identifier: 157963020

GI number: 157963020

Start: 3927359

End: 3928261

Strand: Direct

Name: metR [H]

Synonym: Spea_3203

Alternate gene names: 157963020

Gene position: 3927359-3928261 (Clockwise)

Preceding gene: 157963014

Following gene: 157963025

Centisome position: 75.9

GC content: 46.51

Gene sequence:

>903_bases
ATGATAGAGCTTAGACATTTACGTACGCTAGTTGCCCTAAAGGAAAGCGGCAGCCTTGCTGGGGCAGCAAAAAAACGCTT
CGTCACTCAGTCAGCACTCTCGCATCAGATCAAGGAATTAGAGACTCGTATTAACTCTGCTATTTTTGTACGCAAGAGTA
AGCCGCTCTCTTTCACACAAGAAGGCGCGAGATTACTCAACCTTGCTGAAGATATTCTACCTAAGGTGATCGAAACGGAG
TTTGATCTCAAAAAGGGCCTAGAAGATGGAGATAATCACTTAGGTGTTGGCATCGAATGTCACAGCTGTTTCCGCTGGTT
AATGCCTGTGATGGAAGAGTTCAGAAAGCAATATCCGCAAGCCGAACTCGATTTATCCAGCCGTCATCTGTTCGACTCGT
TGAACGCACTAGAGCAAGGTGATCTGGATGTTGTACTGAGCTCAGATCCTGTGCCTGGTCAGTCAATTGCTTATCAGCAT
CTGTTTGATTTCGAAGTTAGATTAGTGGTGGCCAGTGATCACCCTCTAGCTCAGCAAGAATATGTCACACCGAAACAGTT
AGAGAATTTACCGATTTTAAGCTACCCGGTAGCACTCGAACGCTTAGATATATACCGCCACTTTTTAGAGCCGGCAGGCA
TTCAACCCGGTCCACAAGTAAAGTGTGATTTAACCATGATGCTGTTACAGCGAATTGCCTGTAAAGATGGTGTTGCCGCT
TTGCCAAGCTGGTCGATAAGTGAGGCGCACGGCCTAAGTCTTACCAGTGTAAAACTGGGCCCTGAAGGGCTAAAGCGTCC
ACTTTTTGGGGCGTATCGTCGCAACGGCGCTAATGCTCGTTTAGCGCAACATTGGTTAGAGCTAGTGGCTAAAGAAGGTT
TGGCAAAACAGAAGCTCAATTAA

Upstream 100 bases:

>100_bases
AATGGGCGTCTGGACGTCTAGAAGTTTATTGTGTTAGCCTATGTATTCGCAATCGAAACGAATTCAAGCGCAACATGAGC
GAATTTCATGTGAGGATAAA

Downstream 100 bases:

>100_bases
GTCTGAGTTAGTTAACAAAAAGCCTGCATACTGCAGGCTTTTTTGGTTTGCTATATTGGGTTAAGCAATAAGTTTCTCTT
TGTTAATACCCGCCTTAATA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MIELRHLRTLVALKESGSLAGAAKKRFVTQSALSHQIKELETRINSAIFVRKSKPLSFTQEGARLLNLAEDILPKVIETE
FDLKKGLEDGDNHLGVGIECHSCFRWLMPVMEEFRKQYPQAELDLSSRHLFDSLNALEQGDLDVVLSSDPVPGQSIAYQH
LFDFEVRLVVASDHPLAQQEYVTPKQLENLPILSYPVALERLDIYRHFLEPAGIQPGPQVKCDLTMMLLQRIACKDGVAA
LPSWSISEAHGLSLTSVKLGPEGLKRPLFGAYRRNGANARLAQHWLELVAKEGLAKQKLN

Sequences:

>Translated_300_residues
MIELRHLRTLVALKESGSLAGAAKKRFVTQSALSHQIKELETRINSAIFVRKSKPLSFTQEGARLLNLAEDILPKVIETE
FDLKKGLEDGDNHLGVGIECHSCFRWLMPVMEEFRKQYPQAELDLSSRHLFDSLNALEQGDLDVVLSSDPVPGQSIAYQH
LFDFEVRLVVASDHPLAQQEYVTPKQLENLPILSYPVALERLDIYRHFLEPAGIQPGPQVKCDLTMMLLQRIACKDGVAA
LPSWSISEAHGLSLTSVKLGPEGLKRPLFGAYRRNGANARLAQHWLELVAKEGLAKQKLN
>Mature_300_residues
MIELRHLRTLVALKESGSLAGAAKKRFVTQSALSHQIKELETRINSAIFVRKSKPLSFTQEGARLLNLAEDILPKVIETE
FDLKKGLEDGDNHLGVGIECHSCFRWLMPVMEEFRKQYPQAELDLSSRHLFDSLNALEQGDLDVVLSSDPVPGQSIAYQH
LFDFEVRLVVASDHPLAQQEYVTPKQLENLPILSYPVALERLDIYRHFLEPAGIQPGPQVKCDLTMMLLQRIACKDGVAA
LPSWSISEAHGLSLTSVKLGPEGLKRPLFGAYRRNGANARLAQHWLELVAKEGLAKQKLN

Specific function: Control of the last step in methionine biosynthesis; metR is a positive activator of the metA, metE and metH genes. It is also a negative regulator of its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790262, Length=273, Percent_Identity=45.7875457875458, Blast_Score=242, Evalue=3e-65,
Organism=Escherichia coli, GI1788887, Length=193, Percent_Identity=30.5699481865285, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI87082024, Length=176, Percent_Identity=31.8181818181818, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI157672245, Length=293, Percent_Identity=26.962457337884, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1787601, Length=167, Percent_Identity=29.940119760479, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI145693105, Length=188, Percent_Identity=27.1276595744681, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1786401, Length=218, Percent_Identity=24.3119266055046, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1787879, Length=195, Percent_Identity=26.1538461538462, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI87081904, Length=197, Percent_Identity=28.4263959390863, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1790208, Length=195, Percent_Identity=24.1025641025641, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1788296, Length=188, Percent_Identity=28.1914893617021, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1790399, Length=248, Percent_Identity=25.8064516129032, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33577; Mature: 33577

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIELRHLRTLVALKESGSLAGAAKKRFVTQSALSHQIKELETRINSAIFVRKSKPLSFTQ
CCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCHHH
EGARLLNLAEDILPKVIETEFDLKKGLEDGDNHLGVGIECHSCFRWLMPVMEEFRKQYPQ
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCEEHHHHHHHHHHHHHHHHHHCCC
AELDLSSRHLFDSLNALEQGDLDVVLSSDPVPGQSIAYQHLFDFEVRLVVASDHPLAQQE
CCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHCCEEEEEEEECCCCCCHHH
YVTPKQLENLPILSYPVALERLDIYRHFLEPAGIQPGPQVKCDLTMMLLQRIACKDGVAA
CCCHHHHCCCCCEEHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCC
LPSWSISEAHGLSLTSVKLGPEGLKRPLFGAYRRNGANARLAQHWLELVAKEGLAKQKLN
CCCCCCCCCCCCEEEEEEECHHHHCCHHHHHHHCCCCCHHHHHHHHHHHHHHCHHHHCCC
>Mature Secondary Structure
MIELRHLRTLVALKESGSLAGAAKKRFVTQSALSHQIKELETRINSAIFVRKSKPLSFTQ
CCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCHHH
EGARLLNLAEDILPKVIETEFDLKKGLEDGDNHLGVGIECHSCFRWLMPVMEEFRKQYPQ
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCEEHHHHHHHHHHHHHHHHHHCCC
AELDLSSRHLFDSLNALEQGDLDVVLSSDPVPGQSIAYQHLFDFEVRLVVASDHPLAQQE
CCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHCCEEEEEEEECCCCCCHHH
YVTPKQLENLPILSYPVALERLDIYRHFLEPAGIQPGPQVKCDLTMMLLQRIACKDGVAA
CCCHHHHCCCCCEEHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCC
LPSWSISEAHGLSLTSVKLGPEGLKRPLFGAYRRNGANARLAQHWLELVAKEGLAKQKLN
CCCCCCCCCCCCEEEEEEECHHHHCCHHHHHHHCCCCCHHHHHHHHHHHHHHCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]