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The map label for this gene is lipB [H]

Identifier: 157962972

GI number: 157962972

Start: 3860207

End: 3860866

Strand: Direct

Name: lipB [H]

Synonym: Spea_3155

Alternate gene names: 157962972

Gene position: 3860207-3860866 (Clockwise)

Preceding gene: 157962971

Following gene: 157962973

Centisome position: 74.6

GC content: 45.61

Gene sequence:

>660_bases
TTGTACGATAACGCTTTGCATATTCGTCATCTAGGTAAACAAGATTACGAATCAGTGTGGCACGCGATGCAGCACTACAC
AGATAATCGCGATGAGAACAGCCAAGATGAGATCTGGATTGTTGAACATACGCCAGTTTTCACTCAAGGCCAAGCAGGTA
AGAGTGAGCACATTTTAAACCCTGGTGATATTCCTGTTATACAGGTAGACCGTGGTGGTCAGGTGACTTACCACGGCCCT
GGACAACTCGTAGTTTACCCTCTTCTCGATATTAAACGCCTTAAAGTGGGTGTACGTCAGCTCGTCACTCACATTGAACA
GAGCATCATTAACATGCTCAAACGTTATCAGATTGAAGCTTATGCCAAAGCTGATGCGCCAGGTGTTTATGTTGAAGAAC
GAAAAATAGCATCACTCGGTCTTAGGATCCGTAAAGGGTGCTCTTTCCACGGATTGGCCCTCAATGTCGATATGGATATG
TCACCATTTCAGCGCATAAACCCTTGTGGTTACGCTGGCATGGAAATGATCCAATGCAAACAGCTCGGTGGACCACAAAC
TGTCGAAGAGGCAGGTGCCCAACTGATCGAAACCCTTAGCCAAGAACTCGGTTTAGATAAGCTAGTTCACCACCAAGGAT
TACCAGAGTCATATGAATAG

Upstream 100 bases:

>100_bases
CTATAAATATTAATCATTAATGATAAATGTGATCCGTATTACATTTATCATTTTGAAGCGTATAATAGCCCGCACAAATA
TTTGAGGGGAGAGGTTGCCC

Downstream 100 bases:

>100_bases
GCCTGAAAGATTACAACCTGGCGTTAAACTAAGAGACGCAGACAAGGTTTCCCGCATTCCAGTGAAAGTGGTGCCATCTG
AACGTGACACCATGTTGAGA

Product: lipoate-protein ligase B

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase [H]

Number of amino acids: Translated: 219; Mature: 219

Protein sequence:

>219_residues
MYDNALHIRHLGKQDYESVWHAMQHYTDNRDENSQDEIWIVEHTPVFTQGQAGKSEHILNPGDIPVIQVDRGGQVTYHGP
GQLVVYPLLDIKRLKVGVRQLVTHIEQSIINMLKRYQIEAYAKADAPGVYVEERKIASLGLRIRKGCSFHGLALNVDMDM
SPFQRINPCGYAGMEMIQCKQLGGPQTVEEAGAQLIETLSQELGLDKLVHHQGLPESYE

Sequences:

>Translated_219_residues
MYDNALHIRHLGKQDYESVWHAMQHYTDNRDENSQDEIWIVEHTPVFTQGQAGKSEHILNPGDIPVIQVDRGGQVTYHGP
GQLVVYPLLDIKRLKVGVRQLVTHIEQSIINMLKRYQIEAYAKADAPGVYVEERKIASLGLRIRKGCSFHGLALNVDMDM
SPFQRINPCGYAGMEMIQCKQLGGPQTVEEAGAQLIETLSQELGLDKLVHHQGLPESYE
>Mature_219_residues
MYDNALHIRHLGKQDYESVWHAMQHYTDNRDENSQDEIWIVEHTPVFTQGQAGKSEHILNPGDIPVIQVDRGGQVTYHGP
GQLVVYPLLDIKRLKVGVRQLVTHIEQSIINMLKRYQIEAYAKADAPGVYVEERKIASLGLRIRKGCSFHGLALNVDMDM
SPFQRINPCGYAGMEMIQCKQLGGPQTVEEAGAQLIETLSQELGLDKLVHHQGLPESYE

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG0321

COG function: function code H; Lipoate-protein ligase B

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipB family [H]

Homologues:

Organism=Homo sapiens, GI221554520, Length=124, Percent_Identity=37.9032258064516, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI87081767, Length=199, Percent_Identity=63.3165829145729, Blast_Score=270, Evalue=7e-74,
Organism=Saccharomyces cerevisiae, GI6323268, Length=161, Percent_Identity=32.2981366459627, Blast_Score=86, Evalue=3e-18,
Organism=Drosophila melanogaster, GI21355483, Length=182, Percent_Identity=30.2197802197802, Blast_Score=86, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605 [H]

Pfam domain/function: PF03099 BPL_LipA_LipB [H]

EC number: =2.3.1.181 [H]

Molecular weight: Translated: 24713; Mature: 24713

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: PS01313 LIPB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYDNALHIRHLGKQDYESVWHAMQHYTDNRDENSQDEIWIVEHTPVFTQGQAGKSEHILN
CCCCCCHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCEEECCCCCCCCCCCC
PGDIPVIQVDRGGQVTYHGPGQLVVYPLLDIKRLKVGVRQLVTHIEQSIINMLKRYQIEA
CCCCCEEEECCCCEEEEECCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YAKADAPGVYVEERKIASLGLRIRKGCSFHGLALNVDMDMSPFQRINPCGYAGMEMIQCK
EECCCCCCEEEHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHCCCCCHHHHHHHHHH
QLGGPQTVEEAGAQLIETLSQELGLDKLVHHQGLPESYE
HCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCCCCC
>Mature Secondary Structure
MYDNALHIRHLGKQDYESVWHAMQHYTDNRDENSQDEIWIVEHTPVFTQGQAGKSEHILN
CCCCCCHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCEEECCCCCCCCCCCC
PGDIPVIQVDRGGQVTYHGPGQLVVYPLLDIKRLKVGVRQLVTHIEQSIINMLKRYQIEA
CCCCCEEEECCCCEEEEECCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YAKADAPGVYVEERKIASLGLRIRKGCSFHGLALNVDMDMSPFQRINPCGYAGMEMIQCK
EECCCCCCEEEHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHCCCCCHHHHHHHHHH
QLGGPQTVEEAGAQLIETLSQELGLDKLVHHQGLPESYE
HCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA