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The map label for this gene is mrdA [H]

Identifier: 157962966

GI number: 157962966

Start: 3853607

End: 3855463

Strand: Direct

Name: mrdA [H]

Synonym: Spea_3149

Alternate gene names: 157962966

Gene position: 3853607-3855463 (Clockwise)

Preceding gene: 157962965

Following gene: 157962967

Centisome position: 74.47

GC content: 46.53

Gene sequence:

>1857_bases
GTGTCCCCTAGAAAGCGCATAACCATGAATGACCATGCGGCCGAGGCGTCGCTGTTTAAGAGTCGGGCCCTGTTTACTTT
TTTAAGTGTTATTATCCTGCTGAGTCTGTTGCTGGTTAATCTTTATAATCTGCAAATCACCTCTTTTAAAGACTATGAAA
CCCGCTCAAACGATAATCGTATTCGTGTGGTTCCAGTAGCCCCCAGCCGTGGCCTGATCTACGATCGACACGGGCAACTA
CTTGCCGAAAACCAGCCTTTTTTCTCGCTAGAAATGGTCCCTGAAAAGGTCAAAAATGTCAGCGACACCTTAGATGAACT
CAGTCAAGTGATCGACTTGTCCGCCGATGAAAAAGCCAATGTGCTCGACGCACTCAAGTATCATCGCCGATTTAAACCTC
TGACAATAAAGAACAAGCTCACCGAAGAGCAAGTGGCTGAATTCAGCGTCAACCAACACCGCTTTCCAGGCTTATCTGTC
GAAGCAGGCCTAAAGCGCCATTATCCTTATAATGGCTTGATGACCCATGCCCTCGGTTATGTCGGCAAGATTAACAGCCG
AGATCAAGAATCCCTTATTCGCAATGACCTATGGGGAAACTATGCGGCGACAAAAGATATCGGTAAACAAGGCATAGAAA
AGTTTTACGAAAGTTTACTGTTAGGTAAGCCTGGGCACCTCGAAGAGGAGGTCAATAACCGTGGTCGTACTATTCGCACC
CTGAAAGCTGTACCACCTGAACCGGGGCAGGATATCTACCTAACGTTAGATCTAAAGTTGCAGCAAAAAGCATTAGAATT
ACTTAACGGTCGACGCGGCTCTATTGTTGCAATCGACCCTCGTGACGGCGGTATTTTGGCTCTTGTATCTAGCCCTACCT
ACGATCCAAACCTATTCGTTCACGGCATCAGCAGCAAAGCCTATAGTGAGCTTTTAAACTCTAAGTCTAAACCACTTATT
AATCGCGCCACTCAAGGGCAATATGCACCAGCATCAACGGTAAAACCACATTTAGCGCTACTAGGTTTAGAAAATAAAAC
CGTCACCCCAAAAACTCGTATTTGGGATCCAGGTTTTTGGCAGATCCCCGGTGTTGAACGTAAGTACCGTGACTGGAAAC
GCTGGGGTCACGGCTGGGTCGATGTAAATAGCGCTATTGTAAGCTCCTGTGACACCTATTTTTATGACTTAGCCTATAAG
ACTGGTGTCGACTCGATAGCCAACTTTATGGAGCCCTTTGGTTTTGGTGAACGTACTGGCGTCGATATCTTCGAAGAGTC
AGCTGGTATTATGCCGTCGAAAGACTGGAAACGCCTACGTTACAACCAACCTTGGTATATCGGTGACACCATCTCAGTAG
GGATTGGACAGGGCTACTGGACAACCACACCATTGCAACTTGCCAATGCCACCGCAATTCTAGCGAACAACGGGCGCCGT
TTTGTGCCACATTTTTTAAAGTCTATTCAAGATCAGACTGCTAAAATCGACACGCCACCCGATGAGTTGCCACCCATCGA
ATTAAATGATCCTAATAACTGGAAAATCATCAACGAAGCGATGCGCGATACCGCACATAAGTCACGCTTCACTGACGCTC
GTTATACTGCCGCGATGAAGACAGGTACAGCGCAGGTATTTAGTGTTGCCCAAGACGCTGAGTATGATGCAGATAACATT
GATGAACGCTTACGTGATAACGCATTAATTATCGCTTATGCCCCTTATGAGAACCCAAAGATTGTACTCGCTGTCGTACT
TGAGAATGCTGGTTGGGGCGGTGTAAACGCAGGCCCCGTAGCTCGCGCCTTGTTAGACGAGTATTTATTAAGAGATTCAT
GGGATAAGACTAAATGA

Upstream 100 bases:

>100_bases
CTATCATAGAGAATAATATCGAATCACTATTTGGTTAGACTGACCTTAACAGGACTGGCATATGTGCTTGGCACCCTTTT
TTAAGCAGGAGAGAATGTAA

Downstream 100 bases:

>100_bases
GTGCACACAATCACCGCCCAAATATCTGGCAGCGTCTACACATAGACCTACCTCTGCTGTTAGGCCTTTTAGCACTAATG
GCCTTTGGTCTAGTCGTTAT

Product: peptidoglycan glycosyltransferase

Products: NA

Alternate protein names: PBP-2 [H]

Number of amino acids: Translated: 618; Mature: 617

Protein sequence:

>618_residues
MSPRKRITMNDHAAEASLFKSRALFTFLSVIILLSLLLVNLYNLQITSFKDYETRSNDNRIRVVPVAPSRGLIYDRHGQL
LAENQPFFSLEMVPEKVKNVSDTLDELSQVIDLSADEKANVLDALKYHRRFKPLTIKNKLTEEQVAEFSVNQHRFPGLSV
EAGLKRHYPYNGLMTHALGYVGKINSRDQESLIRNDLWGNYAATKDIGKQGIEKFYESLLLGKPGHLEEEVNNRGRTIRT
LKAVPPEPGQDIYLTLDLKLQQKALELLNGRRGSIVAIDPRDGGILALVSSPTYDPNLFVHGISSKAYSELLNSKSKPLI
NRATQGQYAPASTVKPHLALLGLENKTVTPKTRIWDPGFWQIPGVERKYRDWKRWGHGWVDVNSAIVSSCDTYFYDLAYK
TGVDSIANFMEPFGFGERTGVDIFEESAGIMPSKDWKRLRYNQPWYIGDTISVGIGQGYWTTTPLQLANATAILANNGRR
FVPHFLKSIQDQTAKIDTPPDELPPIELNDPNNWKIINEAMRDTAHKSRFTDARYTAAMKTGTAQVFSVAQDAEYDADNI
DERLRDNALIIAYAPYENPKIVLAVVLENAGWGGVNAGPVARALLDEYLLRDSWDKTK

Sequences:

>Translated_618_residues
MSPRKRITMNDHAAEASLFKSRALFTFLSVIILLSLLLVNLYNLQITSFKDYETRSNDNRIRVVPVAPSRGLIYDRHGQL
LAENQPFFSLEMVPEKVKNVSDTLDELSQVIDLSADEKANVLDALKYHRRFKPLTIKNKLTEEQVAEFSVNQHRFPGLSV
EAGLKRHYPYNGLMTHALGYVGKINSRDQESLIRNDLWGNYAATKDIGKQGIEKFYESLLLGKPGHLEEEVNNRGRTIRT
LKAVPPEPGQDIYLTLDLKLQQKALELLNGRRGSIVAIDPRDGGILALVSSPTYDPNLFVHGISSKAYSELLNSKSKPLI
NRATQGQYAPASTVKPHLALLGLENKTVTPKTRIWDPGFWQIPGVERKYRDWKRWGHGWVDVNSAIVSSCDTYFYDLAYK
TGVDSIANFMEPFGFGERTGVDIFEESAGIMPSKDWKRLRYNQPWYIGDTISVGIGQGYWTTTPLQLANATAILANNGRR
FVPHFLKSIQDQTAKIDTPPDELPPIELNDPNNWKIINEAMRDTAHKSRFTDARYTAAMKTGTAQVFSVAQDAEYDADNI
DERLRDNALIIAYAPYENPKIVLAVVLENAGWGGVNAGPVARALLDEYLLRDSWDKTK
>Mature_617_residues
SPRKRITMNDHAAEASLFKSRALFTFLSVIILLSLLLVNLYNLQITSFKDYETRSNDNRIRVVPVAPSRGLIYDRHGQLL
AENQPFFSLEMVPEKVKNVSDTLDELSQVIDLSADEKANVLDALKYHRRFKPLTIKNKLTEEQVAEFSVNQHRFPGLSVE
AGLKRHYPYNGLMTHALGYVGKINSRDQESLIRNDLWGNYAATKDIGKQGIEKFYESLLLGKPGHLEEEVNNRGRTIRTL
KAVPPEPGQDIYLTLDLKLQQKALELLNGRRGSIVAIDPRDGGILALVSSPTYDPNLFVHGISSKAYSELLNSKSKPLIN
RATQGQYAPASTVKPHLALLGLENKTVTPKTRIWDPGFWQIPGVERKYRDWKRWGHGWVDVNSAIVSSCDTYFYDLAYKT
GVDSIANFMEPFGFGERTGVDIFEESAGIMPSKDWKRLRYNQPWYIGDTISVGIGQGYWTTTPLQLANATAILANNGRRF
VPHFLKSIQDQTAKIDTPPDELPPIELNDPNNWKIINEAMRDTAHKSRFTDARYTAAMKTGTAQVFSVAQDAEYDADNID
ERLRDNALIIAYAPYENPKIVLAVVLENAGWGGVNAGPVARALLDEYLLRDSWDKTK

Specific function: Cell wall formation; PBP-2 is responsible for the determination of the rod shape of the cell. Its synthesize cross- linked peptidoglycan from the lipid intermediates [H]

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786854, Length=613, Percent_Identity=50.4078303425775, Blast_Score=632, Evalue=0.0,
Organism=Escherichia coli, GI1786272, Length=608, Percent_Identity=24.3421052631579, Blast_Score=115, Evalue=9e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR005311
- InterPro:   IPR001460
- InterPro:   IPR017790 [H]

Pfam domain/function: PF03717 PBP_dimer; PF00905 Transpeptidase [H]

EC number: NA

Molecular weight: Translated: 69614; Mature: 69483

Theoretical pI: Translated: 8.28; Mature: 8.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPRKRITMNDHAAEASLFKSRALFTFLSVIILLSLLLVNLYNLQITSFKDYETRSNDNR
CCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCCCCCCCCCE
IRVVPVAPSRGLIYDRHGQLLAENQPFFSLEMVPEKVKNVSDTLDELSQVIDLSADEKAN
EEEEEECCCCCEEEECCCCEEECCCCCEEEECCHHHHHHHHHHHHHHHHHHCCCCCCHHH
VLDALKYHRRFKPLTIKNKLTEEQVAEFSVNQHRFPGLSVEAGLKRHYPYNGLMTHALGY
HHHHHHHHHHCCCEEHHHHCCHHHHHHHCCCCCCCCCCCHHCCHHHCCCCCHHHHHHHHH
VGKINSRDQESLIRNDLWGNYAATKDIGKQGIEKFYESLLLGKPGHLEEEVNNRGRTIRT
HHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCEEEE
LKAVPPEPGQDIYLTLDLKLQQKALELLNGRRGSIVAIDPRDGGILALVSSPTYDPNLFV
EECCCCCCCCEEEEEEEEHHHHHHHHHHCCCCCCEEEECCCCCCEEEEEECCCCCCCEEE
HGISSKAYSELLNSKSKPLINRATQGQYAPASTVKPHLALLGLENKTVTPKTRIWDPGFW
EECCHHHHHHHHCCCCCCHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCCCEECCCCCE
QIPGVERKYRDWKRWGHGWVDVNSAIVSSCDTYFYDLAYKTGVDSIANFMEPFGFGERTG
ECCCCHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCC
VDIFEESAGIMPSKDWKRLRYNQPWYIGDTISVGIGQGYWTTTPLQLANATAILANNGRR
CHHHHHCCCCCCCCCHHHHCCCCCEEECCEEEECCCCCEECCCCHHHCCCEEEEECCCCC
FVPHFLKSIQDQTAKIDTPPDELPPIELNDPNNWKIINEAMRDTAHKSRFTDARYTAAMK
HHHHHHHHHHHHCCCCCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHH
TGTAQVFSVAQDAEYDADNIDERLRDNALIIAYAPYENPKIVLAVVLENAGWGGVNAGPV
CCCHHHHHHHHCCCCCCCHHHHHHCCCEEEEEECCCCCCCEEEEEEEECCCCCCCCHHHH
ARALLDEYLLRDSWDKTK
HHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SPRKRITMNDHAAEASLFKSRALFTFLSVIILLSLLLVNLYNLQITSFKDYETRSNDNR
CCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCCCCCCCCCE
IRVVPVAPSRGLIYDRHGQLLAENQPFFSLEMVPEKVKNVSDTLDELSQVIDLSADEKAN
EEEEEECCCCCEEEECCCCEEECCCCCEEEECCHHHHHHHHHHHHHHHHHHCCCCCCHHH
VLDALKYHRRFKPLTIKNKLTEEQVAEFSVNQHRFPGLSVEAGLKRHYPYNGLMTHALGY
HHHHHHHHHHCCCEEHHHHCCHHHHHHHCCCCCCCCCCCHHCCHHHCCCCCHHHHHHHHH
VGKINSRDQESLIRNDLWGNYAATKDIGKQGIEKFYESLLLGKPGHLEEEVNNRGRTIRT
HHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCEEEE
LKAVPPEPGQDIYLTLDLKLQQKALELLNGRRGSIVAIDPRDGGILALVSSPTYDPNLFV
EECCCCCCCCEEEEEEEEHHHHHHHHHHCCCCCCEEEECCCCCCEEEEEECCCCCCCEEE
HGISSKAYSELLNSKSKPLINRATQGQYAPASTVKPHLALLGLENKTVTPKTRIWDPGFW
EECCHHHHHHHHCCCCCCHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCCCEECCCCCE
QIPGVERKYRDWKRWGHGWVDVNSAIVSSCDTYFYDLAYKTGVDSIANFMEPFGFGERTG
ECCCCHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCC
VDIFEESAGIMPSKDWKRLRYNQPWYIGDTISVGIGQGYWTTTPLQLANATAILANNGRR
CHHHHHCCCCCCCCCHHHHCCCCCEEECCEEEECCCCCEECCCCHHHCCCEEEEECCCCC
FVPHFLKSIQDQTAKIDTPPDELPPIELNDPNNWKIINEAMRDTAHKSRFTDARYTAAMK
HHHHHHHHHHHHCCCCCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHH
TGTAQVFSVAQDAEYDADNIDERLRDNALIIAYAPYENPKIVLAVVLENAGWGGVNAGPV
CCCHHHHHHHHCCCCCCCHHHHHHCCCEEEEEECCCCCCCEEEEEEEECCCCCCCCHHHH
ARALLDEYLLRDSWDKTK
HHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]