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The map label for this gene is rlmH

Identifier: 157962965

GI number: 157962965

Start: 3853052

End: 3853522

Strand: Direct

Name: rlmH

Synonym: Spea_3148

Alternate gene names: 157962965

Gene position: 3853052-3853522 (Clockwise)

Preceding gene: 157962964

Following gene: 157962966

Centisome position: 74.46

GC content: 47.13

Gene sequence:

>471_bases
ATGAAGATTCAGTTAGTCGCAGTTGGAACCCGCATGCCAGATTGGGTTGAAACAGGTTTCAAAGAATATCAGCGCCGCTT
TCCTCGAGATATGGCGCTTGAATTAGTTGAGATCCCTGCTGGCAAACGCGGTAAAAATGCCGATATAGCTCGCATTCTCC
AAAAAGAGGGCGAGTTAATGTTGGCGGCGATCCCGAAAGGGAATCATATCGTCAGCTTAGACTTACCGGGCAAAAACCTC
ACAACACCGCAATTAGCAGAGCAGATGAACAAATGGCTACTCGATGGCCGCGATGTTAGCTTGTTAATTGGAGGCCCAGA
AGGTTTATCTCCTGAGTGCAAAAAAGCCGCAGCGCAAAGCTGGTGCTTATCAGCCTTAACCCTACCTCATCCTTTGGTAC
GCGTGATTGTTGCCGAAAGCCTTTATCGTGGCTGGAGCATTAACAATAATCATCCCTATCATAGAGAATAA

Upstream 100 bases:

>100_bases
GGGTTCTTGTGGACCTAGGCGATGTCATCATGCATGTTATGCAAGATCAAACTCGCGATTTCTACCAGCTTGAAAAACTT
TGGTCAGAAAAGCCTGAGTA

Downstream 100 bases:

>100_bases
TATCGAATCACTATTTGGTTAGACTGACCTTAACAGGACTGGCATATGTGCTTGGCACCCTTTTTTAAGCAGGAGAGAAT
GTAAGTGTCCCCTAGAAAGC

Product: rRNA large subunit methyltransferase

Products: NA

Alternate protein names: 23S rRNA m3Psi1915 methyltransferase; rRNA (pseudouridine-N3-)-methyltransferase rlmH

Number of amino acids: Translated: 156; Mature: 156

Protein sequence:

>156_residues
MKIQLVAVGTRMPDWVETGFKEYQRRFPRDMALELVEIPAGKRGKNADIARILQKEGELMLAAIPKGNHIVSLDLPGKNL
TTPQLAEQMNKWLLDGRDVSLLIGGPEGLSPECKKAAAQSWCLSALTLPHPLVRVIVAESLYRGWSINNNHPYHRE

Sequences:

>Translated_156_residues
MKIQLVAVGTRMPDWVETGFKEYQRRFPRDMALELVEIPAGKRGKNADIARILQKEGELMLAAIPKGNHIVSLDLPGKNL
TTPQLAEQMNKWLLDGRDVSLLIGGPEGLSPECKKAAAQSWCLSALTLPHPLVRVIVAESLYRGWSINNNHPYHRE
>Mature_156_residues
MKIQLVAVGTRMPDWVETGFKEYQRRFPRDMALELVEIPAGKRGKNADIARILQKEGELMLAAIPKGNHIVSLDLPGKNL
TTPQLAEQMNKWLLDGRDVSLLIGGPEGLSPECKKAAAQSWCLSALTLPHPLVRVIVAESLYRGWSINNNHPYHRE

Specific function: Specifically methylates the pseudouridine at position 1915 (m3Psi1915) in 23S rRNA

COG id: COG1576

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase rlmH family

Homologues:

Organism=Escherichia coli, GI1786855, Length=156, Percent_Identity=77.5641025641026, Blast_Score=249, Evalue=4e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMH_SHEHH (B0TR49)

Other databases:

- EMBL:   CP000931
- RefSeq:   YP_001675439.1
- ProteinModelPortal:   B0TR49
- SMR:   B0TR49
- GeneID:   5906430
- GenomeReviews:   CP000931_GR
- KEGG:   shl:Shal_3233
- HOGENOM:   HBG622807
- OMA:   TANHPYH
- ProtClustDB:   PRK00103
- BioCyc:   SHAL458817:SHAL_3233-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00658
- InterPro:   IPR016051
- InterPro:   IPR003742
- PIRSF:   PIRSF004505
- TIGRFAMs:   TIGR00246

Pfam domain/function: PF02590 SPOUT_MTase

EC number: NA

Molecular weight: Translated: 17446; Mature: 17446

Theoretical pI: Translated: 9.18; Mature: 9.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIQLVAVGTRMPDWVETGFKEYQRRFPRDMALELVEIPAGKRGKNADIARILQKEGELM
CEEEEEEECCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCEE
LAAIPKGNHIVSLDLPGKNLTTPQLAEQMNKWLLDGRDVSLLIGGPEGLSPECKKAAAQS
EEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHH
WCLSALTLPHPLVRVIVAESLYRGWSINNNHPYHRE
HHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MKIQLVAVGTRMPDWVETGFKEYQRRFPRDMALELVEIPAGKRGKNADIARILQKEGELM
CEEEEEEECCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCEE
LAAIPKGNHIVSLDLPGKNLTTPQLAEQMNKWLLDGRDVSLLIGGPEGLSPECKKAAAQS
EEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHH
WCLSALTLPHPLVRVIVAESLYRGWSINNNHPYHRE
HHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA