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The map label for this gene is lutB [H]

Identifier: 157962815

GI number: 157962815

Start: 3672701

End: 3674074

Strand: Direct

Name: lutB [H]

Synonym: Spea_2997

Alternate gene names: 157962815

Gene position: 3672701-3674074 (Clockwise)

Preceding gene: 157962814

Following gene: 157962816

Centisome position: 70.98

GC content: 49.93

Gene sequence:

>1374_bases
ATGTCCACCTCTAATTCAGCGGTACACGCCCATAAGGCGGAGATCTTCTGTAAAGATGAATCCCGCGTTGATTGGCACTC
TAAAGCCCTTTGGCTACTGCGTGAAAAACGCGACCGCGCGGCGGGCAGTTTGCCTGAGTGGGAACAGTTACGCCAAATTG
GCTCGGAGATGAAGCTTCATACGCTGACCCACTTGGGCCAGTACTTAGAAGAGTTTGAGCAAAACTGCATAAAGAACAAT
ATTCAAGTGCATTGGGCCAGAGATGGTGTAGAACACAATCGTATCGTGCATGAGATTTTGGCCAAACATCAGGTCAAGAA
GTTAGTTAAGTCTAAATCTATGCTCACCGAAGAGTGCCATATGAATCCATATCTGGAGCAACATGGCATTGAGGTCATTG
ATACCGATTTAGGCGAACGCATTATTCAGCTTGCAGGCCAGCCACCATCACACATTGTTGTGCCTGCTATTCACTTGAAA
AAAGAGGAAGTCGGCGATCTATTCCATGACAAACTTGGCACTGACGCCGGCGCTTCTGACCCACTTTATCTAACCCGCGC
CGCCCGCGCACACTTGCGTGAACAATTCTTGTCTGCCGATGCGGCGATGACAGGGGTGAATATGGCGATTGCCGATAAAG
GGGCTGTGGTAGTGTGCACCAACGAGGGCAACGCCGACATGGGCGCCAACCTGCCAAAGCTGCAACTTCATTCGATGGGC
ATAGACAAGCTAGTGCCTAATATTGATAGCGCGGCAGTTCTGCTGCGTACGCTTGCCAGAAACGCAACTGGTCAGCCTAT
TACCACCTACAGCTCTTTTTATCGCGGCCCACAAGATGGCGGCGAGATGCATGTGATTATTGTCGATAACGGCCGTACTG
ACATGCTAAAAGACAAAATCTTAGCCGAATCTTTAAAATGTATTCGCTGTGGTGGCTGCCTTAACACCTGTCCTGTGTAT
CGCCGCAGCGGCGGTTACAGTTACAACTACACCATTCCAGGGCCAATCGGTATCGCGGTGGGCTCGCAAAGGGATGATAC
CAACTCAATCCCTTGGGCATGTACCTTATGTGGCAGCTGCTCTTATGTTTGTCCAACTAAGGTACCACTGGATAAAATCA
TCCATCATCATCGCCGCCTAAAAGCCGAGGCAGGTAAACTCCCCTACGGCAAAGCCAGCTATATGCCGCTAGTCGGCAAG
TTTATGGCCAGTGAAACGGCTCTTAATTGCTCGATGAGCGTTGCTCGTATAGCGCTTAGGATTTTACCGGGTAGCCTATT
AAAACCATTTTCAGGCGCTTGGGGGAAATACCGTGAGCTACCCGTAGCCCCCAACTCAAGCTTCGAAGCTTGGTTTAAGA
AAAACAGAGGGTAG

Upstream 100 bases:

>100_bases
CTTGCCTGCTGCACATCGATGGTATGGTCAGACGACAGAAACTGCCAATTGAGACTCGTCATATCGCCCAAGTTATCAAC
GCAGCGCTTTAGGAGAAGAT

Downstream 100 bases:

>100_bases
AAATATGTCTAGCAAACACGACATTCTTAACGCATTAAAAAGCGCCGCGATTGCGCCTCAATCCATGCCTGTTATCGATA
TTTCGCCGCGAATCGACGAT

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 457; Mature: 456

Protein sequence:

>457_residues
MSTSNSAVHAHKAEIFCKDESRVDWHSKALWLLREKRDRAAGSLPEWEQLRQIGSEMKLHTLTHLGQYLEEFEQNCIKNN
IQVHWARDGVEHNRIVHEILAKHQVKKLVKSKSMLTEECHMNPYLEQHGIEVIDTDLGERIIQLAGQPPSHIVVPAIHLK
KEEVGDLFHDKLGTDAGASDPLYLTRAARAHLREQFLSADAAMTGVNMAIADKGAVVVCTNEGNADMGANLPKLQLHSMG
IDKLVPNIDSAAVLLRTLARNATGQPITTYSSFYRGPQDGGEMHVIIVDNGRTDMLKDKILAESLKCIRCGGCLNTCPVY
RRSGGYSYNYTIPGPIGIAVGSQRDDTNSIPWACTLCGSCSYVCPTKVPLDKIIHHHRRLKAEAGKLPYGKASYMPLVGK
FMASETALNCSMSVARIALRILPGSLLKPFSGAWGKYRELPVAPNSSFEAWFKKNRG

Sequences:

>Translated_457_residues
MSTSNSAVHAHKAEIFCKDESRVDWHSKALWLLREKRDRAAGSLPEWEQLRQIGSEMKLHTLTHLGQYLEEFEQNCIKNN
IQVHWARDGVEHNRIVHEILAKHQVKKLVKSKSMLTEECHMNPYLEQHGIEVIDTDLGERIIQLAGQPPSHIVVPAIHLK
KEEVGDLFHDKLGTDAGASDPLYLTRAARAHLREQFLSADAAMTGVNMAIADKGAVVVCTNEGNADMGANLPKLQLHSMG
IDKLVPNIDSAAVLLRTLARNATGQPITTYSSFYRGPQDGGEMHVIIVDNGRTDMLKDKILAESLKCIRCGGCLNTCPVY
RRSGGYSYNYTIPGPIGIAVGSQRDDTNSIPWACTLCGSCSYVCPTKVPLDKIIHHHRRLKAEAGKLPYGKASYMPLVGK
FMASETALNCSMSVARIALRILPGSLLKPFSGAWGKYRELPVAPNSSFEAWFKKNRG
>Mature_456_residues
STSNSAVHAHKAEIFCKDESRVDWHSKALWLLREKRDRAAGSLPEWEQLRQIGSEMKLHTLTHLGQYLEEFEQNCIKNNI
QVHWARDGVEHNRIVHEILAKHQVKKLVKSKSMLTEECHMNPYLEQHGIEVIDTDLGERIIQLAGQPPSHIVVPAIHLKK
EEVGDLFHDKLGTDAGASDPLYLTRAARAHLREQFLSADAAMTGVNMAIADKGAVVVCTNEGNADMGANLPKLQLHSMGI
DKLVPNIDSAAVLLRTLARNATGQPITTYSSFYRGPQDGGEMHVIIVDNGRTDMLKDKILAESLKCIRCGGCLNTCPVYR
RSGGYSYNYTIPGPIGIAVGSQRDDTNSIPWACTLCGSCSYVCPTKVPLDKIIHHHRRLKAEAGKLPYGKASYMPLVGKF
MASETALNCSMSVARIALRILPGSLLKPFSGAWGKYRELPVAPNSSFEAWFKKNRG

Specific function: Is involved in L-lactate degradation and allows cells to grow with lactate as the sole carbon source. Has probably a role as an electron transporter during oxidation of L-lactate [H]

COG id: COG1139

COG function: function code C; Uncharacterized conserved protein containing a ferredoxin-like domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1786498, Length=434, Percent_Identity=37.5576036866359, Blast_Score=306, Evalue=2e-84,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR003741
- InterPro:   IPR004452
- InterPro:   IPR002698
- InterPro:   IPR012285
- InterPro:   IPR009051
- InterPro:   IPR022825 [H]

Pfam domain/function: PF02589 DUF162 [H]

EC number: NA

Molecular weight: Translated: 50581; Mature: 50450

Theoretical pI: Translated: 8.48; Mature: 8.48

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTSNSAVHAHKAEIFCKDESRVDWHSKALWLLREKRDRAAGSLPEWEQLRQIGSEMKLH
CCCCCCCEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
TLTHLGQYLEEFEQNCIKNNIQVHWARDGVEHNRIVHEILAKHQVKKLVKSKSMLTEECH
HHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
MNPYLEQHGIEVIDTDLGERIIQLAGQPPSHIVVPAIHLKKEEVGDLFHDKLGTDAGASD
CCCCHHHCCCEEEEHHHHHHHHHHCCCCCCCEEEEEHHCCHHHHHHHHHHHHCCCCCCCC
PLYLTRAARAHLREQFLSADAAMTGVNMAIADKGAVVVCTNEGNADMGANLPKLQLHSMG
CCHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCEEEEEECCCCCCCCCCCCCHHHHHCC
IDKLVPNIDSAAVLLRTLARNATGQPITTYSSFYRGPQDGGEMHVIIVDNGRTDMLKDKI
HHHHCCCCCHHHHHHHHHHHCCCCCCEEHHHHHHCCCCCCCCEEEEEEECCCHHHHHHHH
LAESLKCIRCGGCLNTCPVYRRSGGYSYNYTIPGPIGIAVGSQRDDTNSIPWACTLCGSC
HHHHHHHHHCCCCCCCCCHHCCCCCCEEEEECCCCEEEEECCCCCCCCCCCEEEEECCCC
SYVCPTKVPLDKIIHHHRRLKAEAGKLPYGKASYMPLVGKFMASETALNCSMSVARIALR
CCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ILPGSLLKPFSGAWGKYRELPVAPNSSFEAWFKKNRG
HCCCHHHCCCCCCCCCCCCCCCCCCCCHHHHHHCCCC
>Mature Secondary Structure 
STSNSAVHAHKAEIFCKDESRVDWHSKALWLLREKRDRAAGSLPEWEQLRQIGSEMKLH
CCCCCCEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
TLTHLGQYLEEFEQNCIKNNIQVHWARDGVEHNRIVHEILAKHQVKKLVKSKSMLTEECH
HHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
MNPYLEQHGIEVIDTDLGERIIQLAGQPPSHIVVPAIHLKKEEVGDLFHDKLGTDAGASD
CCCCHHHCCCEEEEHHHHHHHHHHCCCCCCCEEEEEHHCCHHHHHHHHHHHHCCCCCCCC
PLYLTRAARAHLREQFLSADAAMTGVNMAIADKGAVVVCTNEGNADMGANLPKLQLHSMG
CCHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCEEEEEECCCCCCCCCCCCCHHHHHCC
IDKLVPNIDSAAVLLRTLARNATGQPITTYSSFYRGPQDGGEMHVIIVDNGRTDMLKDKI
HHHHCCCCCHHHHHHHHHHHCCCCCCEEHHHHHHCCCCCCCCEEEEEEECCCHHHHHHHH
LAESLKCIRCGGCLNTCPVYRRSGGYSYNYTIPGPIGIAVGSQRDDTNSIPWACTLCGSC
HHHHHHHHHCCCCCCCCCHHCCCCCCEEEEECCCCEEEEECCCCCCCCCCCEEEEECCCC
SYVCPTKVPLDKIIHHHRRLKAEAGKLPYGKASYMPLVGKFMASETALNCSMSVARIALR
CCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ILPGSLLKPFSGAWGKYRELPVAPNSSFEAWFKKNRG
HCCCHHHCCCCCCCCCCCCCCCCCCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA