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The map label for this gene is pleD [H]

Identifier: 157962792

GI number: 157962792

Start: 3643872

End: 3645428

Strand: Direct

Name: pleD [H]

Synonym: Spea_2974

Alternate gene names: 157962792

Gene position: 3643872-3645428 (Clockwise)

Preceding gene: 157962791

Following gene: 157962793

Centisome position: 70.42

GC content: 40.72

Gene sequence:

>1557_bases
ATGAAGGATGTCGCCATAAGTACGTCTCAAATAAGCTTGTTACAGCAAAAACTCTTTGCTGCTAGACAGGCATTAGAAGA
GATGACCGAAGAGCAAAATGAAAAGCTACACATCCTCATACAGTTTATTGGACAACTAAGCCTCGCCTGTAAAGGTCAAA
ATCTAGAGCTTGATAATAAGCTCGCTAAATTGCGTCATAAATTAGTAAAGTTTAACCAATTCGAAGATTCACTTCCCGCT
CTTAATGAAGCCGATACCATCCTTAAGCAGCAATATGCCCGTGTGATGCTGCAGCTCGAAGATAGCCGCCAATCATTAGC
GAATATGGTGAGTCAAATTCAGCGTATCGACTCGCTTCCAAGTAAGTTAAAGCTAGAAATTACTTACTTTAAAAAAGAGC
TAATTAAGCCGTTTCACACCCACTGGGATTACATTCCAAAGGTGGAGAAGGTGGTTAATTTTTATGATGAGATCTTAAAA
GAAAAACTTAACCTTGGTGAACAGTTCGAGATCTTACCTCGTCATCGACAAGCCGCTCATGAGCTTGCACTGCTGTTATC
AGAAATAGAGTTTAGAAAAGAGCAACGACAACAGATCTCAGAAATGAAGAAGTCGTTAACCGAAGAGTTTGAACTCGACA
CCTTGCTTGAAGCTTATCAGGTGGTACTTAATCTACTACTGGATAACATTGCTAGAGAAAAAACCGCCTCGCAAGAGTTT
CTATACGTACTCAATAATGCACTGAGCAATGTTCAAGAGGTAGTCACAGATAGCTACAGTCACTCGGTAAAGAATTTTCA
AGTGACCAAGCAGCTTAATCGCGATATCAATTTCCAAGTTGATAACGTTGGCGAAGCCGTGATTGAAGTCGATGATATTG
ATGAGTTAAAAAACCAAATAGCCCTTCATTTATCCTCTTTGCGAAAATCTCTTGGGCGTAAAGAACTGCTCGAAAATAGA
GAGCAAACCCAGCTAAAGCAATCTGTTGAGGCCTTACGTCAAGAACTCAAAGCCCTTGGAAAAGAAACTGACTCATACAA
AGAGCGCTTATTTGAGCAGCAAAAGCTTAATCTATTAGACTCATTAACCCAGCTACCTAATCGCAGCGCATTAGAAGAGC
GCATGGATATTGAATATCGCAACTATAAGCGAACCAAACAGCCATTATGGGTCGCAGTCGCCGATATCGATCACTTCAAA
ACAATTAATGACAGTTTCGGCCATAGTACTGGGGATAAAACACTGCAAGTAATTGCTATGGCGCTGAAAAATGCCTTAAG
AGAGTCGGAGTTTGTCGCACGTTATGGCGGTGAAGAGTTTGTACTCTTGCTGCCTAATGTTGCCGAGAGTGACATCCAAC
CTTTACTTAACCGTGTGAGAGAAAAAGTAAAAAGCATACCGTTTAAGTTTAAAAATCAGCGAATTACAGTTACAGTATCT
ATAGGTGCTGCGCAAATAATCGAAAATGAGCTCATCACAGAAACATTCGACCGAGCCGACGCTGCGCTTTATAAAGCCAA
AAATGAAAGCAGAGATAGGGTCATCATTGACTTCTAA

Upstream 100 bases:

>100_bases
GCCGATGTTGATTTCTCTATTTTGAAGACTACTGATAAGATAAGTGATTAAATTTAAGCTTCATTTTTAAATTGCGCAAT
ATATAGGAACCCAGATAAGC

Downstream 100 bases:

>100_bases
GTTTCTCCCCTTGTGATAACACCCGTTACCACTCTCTCTTTAAGGGACAGCTTGCTTATCACTGCTTAAGGAGTTGCTAT
GATAGTAAAAATAAGCCCAA

Product: diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 518; Mature: 518

Protein sequence:

>518_residues
MKDVAISTSQISLLQQKLFAARQALEEMTEEQNEKLHILIQFIGQLSLACKGQNLELDNKLAKLRHKLVKFNQFEDSLPA
LNEADTILKQQYARVMLQLEDSRQSLANMVSQIQRIDSLPSKLKLEITYFKKELIKPFHTHWDYIPKVEKVVNFYDEILK
EKLNLGEQFEILPRHRQAAHELALLLSEIEFRKEQRQQISEMKKSLTEEFELDTLLEAYQVVLNLLLDNIAREKTASQEF
LYVLNNALSNVQEVVTDSYSHSVKNFQVTKQLNRDINFQVDNVGEAVIEVDDIDELKNQIALHLSSLRKSLGRKELLENR
EQTQLKQSVEALRQELKALGKETDSYKERLFEQQKLNLLDSLTQLPNRSALEERMDIEYRNYKRTKQPLWVAVADIDHFK
TINDSFGHSTGDKTLQVIAMALKNALRESEFVARYGGEEFVLLLPNVAESDIQPLLNRVREKVKSIPFKFKNQRITVTVS
IGAAQIIENELITETFDRADAALYKAKNESRDRVIIDF

Sequences:

>Translated_518_residues
MKDVAISTSQISLLQQKLFAARQALEEMTEEQNEKLHILIQFIGQLSLACKGQNLELDNKLAKLRHKLVKFNQFEDSLPA
LNEADTILKQQYARVMLQLEDSRQSLANMVSQIQRIDSLPSKLKLEITYFKKELIKPFHTHWDYIPKVEKVVNFYDEILK
EKLNLGEQFEILPRHRQAAHELALLLSEIEFRKEQRQQISEMKKSLTEEFELDTLLEAYQVVLNLLLDNIAREKTASQEF
LYVLNNALSNVQEVVTDSYSHSVKNFQVTKQLNRDINFQVDNVGEAVIEVDDIDELKNQIALHLSSLRKSLGRKELLENR
EQTQLKQSVEALRQELKALGKETDSYKERLFEQQKLNLLDSLTQLPNRSALEERMDIEYRNYKRTKQPLWVAVADIDHFK
TINDSFGHSTGDKTLQVIAMALKNALRESEFVARYGGEEFVLLLPNVAESDIQPLLNRVREKVKSIPFKFKNQRITVTVS
IGAAQIIENELITETFDRADAALYKAKNESRDRVIIDF
>Mature_518_residues
MKDVAISTSQISLLQQKLFAARQALEEMTEEQNEKLHILIQFIGQLSLACKGQNLELDNKLAKLRHKLVKFNQFEDSLPA
LNEADTILKQQYARVMLQLEDSRQSLANMVSQIQRIDSLPSKLKLEITYFKKELIKPFHTHWDYIPKVEKVVNFYDEILK
EKLNLGEQFEILPRHRQAAHELALLLSEIEFRKEQRQQISEMKKSLTEEFELDTLLEAYQVVLNLLLDNIAREKTASQEF
LYVLNNALSNVQEVVTDSYSHSVKNFQVTKQLNRDINFQVDNVGEAVIEVDDIDELKNQIALHLSSLRKSLGRKELLENR
EQTQLKQSVEALRQELKALGKETDSYKERLFEQQKLNLLDSLTQLPNRSALEERMDIEYRNYKRTKQPLWVAVADIDHFK
TINDSFGHSTGDKTLQVIAMALKNALRESEFVARYGGEEFVLLLPNVAESDIQPLLNRVREKVKSIPFKFKNQRITVTVS
IGAAQIIENELITETFDRADAALYKAKNESRDRVIIDF

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=157, Percent_Identity=37.5796178343949, Blast_Score=105, Evalue=8e-24,
Organism=Escherichia coli, GI87081881, Length=156, Percent_Identity=37.8205128205128, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1787802, Length=160, Percent_Identity=36.875, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1787816, Length=208, Percent_Identity=29.3269230769231, Blast_Score=96, Evalue=6e-21,
Organism=Escherichia coli, GI1787262, Length=167, Percent_Identity=34.1317365269461, Blast_Score=96, Evalue=8e-21,
Organism=Escherichia coli, GI1788381, Length=158, Percent_Identity=34.1772151898734, Blast_Score=92, Evalue=7e-20,
Organism=Escherichia coli, GI1787541, Length=188, Percent_Identity=29.7872340425532, Blast_Score=92, Evalue=8e-20,
Organism=Escherichia coli, GI1786584, Length=173, Percent_Identity=34.1040462427746, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI87082007, Length=159, Percent_Identity=32.0754716981132, Blast_Score=86, Evalue=8e-18,
Organism=Escherichia coli, GI1788956, Length=164, Percent_Identity=33.5365853658537, Blast_Score=79, Evalue=9e-16,
Organism=Escherichia coli, GI87081977, Length=147, Percent_Identity=31.2925170068027, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI87081974, Length=167, Percent_Identity=26.3473053892216, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 60059; Mature: 60059

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDVAISTSQISLLQQKLFAARQALEEMTEEQNEKLHILIQFIGQLSLACKGQNLELDNK
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCHHHH
LAKLRHKLVKFNQFEDSLPALNEADTILKQQYARVMLQLEDSRQSLANMVSQIQRIDSLP
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SKLKLEITYFKKELIKPFHTHWDYIPKVEKVVNFYDEILKEKLNLGEQFEILPRHRQAAH
HHHEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
ELALLLSEIEFRKEQRQQISEMKKSLTEEFELDTLLEAYQVVLNLLLDNIAREKTASQEF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LYVLNNALSNVQEVVTDSYSHSVKNFQVTKQLNRDINFQVDNVGEAVIEVDDIDELKNQI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCHHEECCCHHHHHHHH
ALHLSSLRKSLGRKELLENREQTQLKQSVEALRQELKALGKETDSYKERLFEQQKLNLLD
HHHHHHHHHHHCHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
SLTQLPNRSALEERMDIEYRNYKRTKQPLWVAVADIDHFKTINDSFGHSTGDKTLQVIAM
HHHHCCCHHHHHHHHCCHHHHHHCCCCCCEEEEECHHHHHHHCHHCCCCCCHHHHHHHHH
ALKNALRESEFVARYGGEEFVLLLPNVAESDIQPLLNRVREKVKSIPFKFKNQRITVTVS
HHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEECCCEEEEEEE
IGAAQIIENELITETFDRADAALYKAKNESRDRVIIDF
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEC
>Mature Secondary Structure
MKDVAISTSQISLLQQKLFAARQALEEMTEEQNEKLHILIQFIGQLSLACKGQNLELDNK
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCHHHH
LAKLRHKLVKFNQFEDSLPALNEADTILKQQYARVMLQLEDSRQSLANMVSQIQRIDSLP
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SKLKLEITYFKKELIKPFHTHWDYIPKVEKVVNFYDEILKEKLNLGEQFEILPRHRQAAH
HHHEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
ELALLLSEIEFRKEQRQQISEMKKSLTEEFELDTLLEAYQVVLNLLLDNIAREKTASQEF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LYVLNNALSNVQEVVTDSYSHSVKNFQVTKQLNRDINFQVDNVGEAVIEVDDIDELKNQI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCHHEECCCHHHHHHHH
ALHLSSLRKSLGRKELLENREQTQLKQSVEALRQELKALGKETDSYKERLFEQQKLNLLD
HHHHHHHHHHHCHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
SLTQLPNRSALEERMDIEYRNYKRTKQPLWVAVADIDHFKTINDSFGHSTGDKTLQVIAM
HHHHCCCHHHHHHHHCCHHHHHHCCCCCCEEEEECHHHHHHHCHHCCCCCCHHHHHHHHH
ALKNALRESEFVARYGGEEFVLLLPNVAESDIQPLLNRVREKVKSIPFKFKNQRITVTVS
HHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCEECCCEEEEEEE
IGAAQIIENELITETFDRADAALYKAKNESRDRVIIDF
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA