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The map label for this gene is pksE [H]

Identifier: 157962729

GI number: 157962729

Start: 3560820

End: 3562448

Strand: Direct

Name: pksE [H]

Synonym: Spea_2910

Alternate gene names: 157962729

Gene position: 3560820-3562448 (Clockwise)

Preceding gene: 157962728

Following gene: 157962730

Centisome position: 68.81

GC content: 51.26

Gene sequence:

>1629_bases
ATGAATCCTACTACAACCAGCGAAATGCTTTCACCGTGGCCATGGGCTGTGACAGAGTCAAATATTAGCTTTGACGTGCA
AGTGATGGAGCAGCAGTTAAAAGACTTTAGCCGTGGCTGCTACGTGGTAAATCACAGCGAAAATGGTGTTGGTATCGCCC
AGTCTGCAGCAATCGTATCAGAGCAGGCAGCTAACAACACAGGGCTGCCAGTCAGTGCCTTTGCCCCTGCTCTTGGTACA
GAAAGCCTAGGCGACAGTAATTTCCGCCGCGTTCACGGTGTTAAATACGCCTACTACGCGGGCGCAATGGCGAATGGTAT
TTCATCGGAAGAGCTGGTTATCGCACTGGGTAACGCCGGTATTTTATGTTCATTTGGCGCAGCGGGTCTTATTCCAAGCC
GCGTCGAACAGGCGATAAACCGCATCCAAGCAGCGCTGCCAAACGGTCCATACATGTTTAACCTTATCCATAGCCCAAGT
GAGCCAGCGCTAGAGCGCGGCAGCGTTGAGCTATTTTTAAAGCATAAGGTTCGCACCGTTGAAGCATCGGCGTTCTTAGG
CTTAACGCCACAAATCGTTTATTACCGCGCCGCAGGCCTAAGCCGCGATGCCCAAGGTAAAATCGTTGTCGCTAACAAGG
TTATCGCTAAAGTAAGCCGCACTGAAGTAGCTGAAAAATTCATGATGCCTGCACCAGCCAAGATGCTACAAAAACTGGTT
GATGACGGTTCAATCACGCCTGAGCAGATGGAGCTTGCGCTACTTGTCCCTATGGCCGACGACATCACTGCAGAAGCCGA
CTCTGGTGGTCACACAGATAACCGTCCATTGGTCACATTGCTGCCGACTATCTTGGCACTAAAAGATGAGATCCAAGCAA
AATTCAATTACCCAACACCAATCCGCGTCGGTTGTGGTGGCGGTGTTGGTACACCCGATGCAGCGCTTGCGACCTTCAAC
ATGGGCGCAGCCTATATTGTGACTGGTTCGGTTAACCAAGCCTGCGTCGAAGCCGGCGCTAGCGATCATACCCGTAAACT
GCTAGCGACTACTGAGATGGCCGATGTCACCATGGCGCCAGCGGCTGATATGTTCGAGATGGGCGTTAAGCTACAGGTGG
TTAAGCGCGGTACGCTTTTCCCAATGCGAGCTAATAAACTGTACGAGATCTACACCCGTTACGACTCAATCGAAGCGATT
CCGGCGGATGAACGCGAAAAGTTAGAGAAGCAAGTATTCCGCTCAAGCCTTGATGAAATCTGGGCGGGCACAGTTGCACA
CTTTAATGAGCGCGATCCTAAGCAGATTGAGCGCGCCGAGGGTAACCCAAAGCGTAAGATGGCACTTATCTTCCGCTGGT
ACCTAGGGCTTTCGAGCCGCTGGTCTAATTCTGGCGAAGTAGGCCGTGAGATGGATTACCAAATTTGGGCTGGCCCTGCA
CTTGGGGCGTTTAACCAATGGGCTAAAGGCAGTTACCTAGATAACTACCAAGACCGTAATGCCGTCGACGTAGCTAAGCA
CCTAATGTATGGCGCGGCTTACCTAAGCCGAATTAACTCATTAACTGCACAAGGCGTTAAACTACCAGCAAGCCTATTAC
GCTGGAAGCCGACCCAAAGAATGGCTTAA

Upstream 100 bases:

>100_bases
TCCTTCGAAGTACAAGAATCAAAGATTAATTAGTGTGAAAGAGACTGATAAACAGCTTCACACTGAACTAAAAGAATAAA
TTACTAAGGGTCTACACATA

Downstream 100 bases:

>100_bases
GGGCAGCCTTGAGCGGCTCGTAGAATGTAGAGAGTCAGCCTTGAGTAGCTTTTATAAAAAGACAAACCAACCTAAAAGCC
ACTAATCTCCATTAGTGGCT

Product: PfaD family protein

Products: NA

Alternate protein names: Malonyl CoA-acyl carrier protein transacylase; MCT [H]

Number of amino acids: Translated: 542; Mature: 542

Protein sequence:

>542_residues
MNPTTTSEMLSPWPWAVTESNISFDVQVMEQQLKDFSRGCYVVNHSENGVGIAQSAAIVSEQAANNTGLPVSAFAPALGT
ESLGDSNFRRVHGVKYAYYAGAMANGISSEELVIALGNAGILCSFGAAGLIPSRVEQAINRIQAALPNGPYMFNLIHSPS
EPALERGSVELFLKHKVRTVEASAFLGLTPQIVYYRAAGLSRDAQGKIVVANKVIAKVSRTEVAEKFMMPAPAKMLQKLV
DDGSITPEQMELALLVPMADDITAEADSGGHTDNRPLVTLLPTILALKDEIQAKFNYPTPIRVGCGGGVGTPDAALATFN
MGAAYIVTGSVNQACVEAGASDHTRKLLATTEMADVTMAPAADMFEMGVKLQVVKRGTLFPMRANKLYEIYTRYDSIEAI
PADEREKLEKQVFRSSLDEIWAGTVAHFNERDPKQIERAEGNPKRKMALIFRWYLGLSSRWSNSGEVGREMDYQIWAGPA
LGAFNQWAKGSYLDNYQDRNAVDVAKHLMYGAAYLSRINSLTAQGVKLPASLLRWKPTQRMA

Sequences:

>Translated_542_residues
MNPTTTSEMLSPWPWAVTESNISFDVQVMEQQLKDFSRGCYVVNHSENGVGIAQSAAIVSEQAANNTGLPVSAFAPALGT
ESLGDSNFRRVHGVKYAYYAGAMANGISSEELVIALGNAGILCSFGAAGLIPSRVEQAINRIQAALPNGPYMFNLIHSPS
EPALERGSVELFLKHKVRTVEASAFLGLTPQIVYYRAAGLSRDAQGKIVVANKVIAKVSRTEVAEKFMMPAPAKMLQKLV
DDGSITPEQMELALLVPMADDITAEADSGGHTDNRPLVTLLPTILALKDEIQAKFNYPTPIRVGCGGGVGTPDAALATFN
MGAAYIVTGSVNQACVEAGASDHTRKLLATTEMADVTMAPAADMFEMGVKLQVVKRGTLFPMRANKLYEIYTRYDSIEAI
PADEREKLEKQVFRSSLDEIWAGTVAHFNERDPKQIERAEGNPKRKMALIFRWYLGLSSRWSNSGEVGREMDYQIWAGPA
LGAFNQWAKGSYLDNYQDRNAVDVAKHLMYGAAYLSRINSLTAQGVKLPASLLRWKPTQRMA
>Mature_542_residues
MNPTTTSEMLSPWPWAVTESNISFDVQVMEQQLKDFSRGCYVVNHSENGVGIAQSAAIVSEQAANNTGLPVSAFAPALGT
ESLGDSNFRRVHGVKYAYYAGAMANGISSEELVIALGNAGILCSFGAAGLIPSRVEQAINRIQAALPNGPYMFNLIHSPS
EPALERGSVELFLKHKVRTVEASAFLGLTPQIVYYRAAGLSRDAQGKIVVANKVIAKVSRTEVAEKFMMPAPAKMLQKLV
DDGSITPEQMELALLVPMADDITAEADSGGHTDNRPLVTLLPTILALKDEIQAKFNYPTPIRVGCGGGVGTPDAALATFN
MGAAYIVTGSVNQACVEAGASDHTRKLLATTEMADVTMAPAADMFEMGVKLQVVKRGTLFPMRANKLYEIYTRYDSIEAI
PADEREKLEKQVFRSSLDEIWAGTVAHFNERDPKQIERAEGNPKRKMALIFRWYLGLSSRWSNSGEVGREMDYQIWAGPA
LGAFNQWAKGSYLDNYQDRNAVDVAKHLMYGAAYLSRINSLTAQGVKLPASLLRWKPTQRMA

Specific function: Probably involved in some intermediate steps for the synthesis of the antibiotic polyketide bacillaene which is involved in secondary metabolism. Probably has an acyl transferase activity and could also have a flavin mononucleotide-dependent oxidoreductas

COG id: COG2070

COG function: function code R; Dioxygenases related to 2-nitropropane dioxygenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: In the N-terminal section; belongs to the fabD family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004136
- InterPro:   IPR001227
- InterPro:   IPR014043
- InterPro:   IPR016035
- InterPro:   IPR013785
- InterPro:   IPR004410
- InterPro:   IPR016036
- InterPro:   IPR014179 [H]

Pfam domain/function: PF00698 Acyl_transf_1; PF03060 NPD [H]

EC number: =2.3.1.39 [H]

Molecular weight: Translated: 58993; Mature: 58993

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPTTTSEMLSPWPWAVTESNISFDVQVMEQQLKDFSRGCYVVNHSENGVGIAQSAAIVS
CCCCCCHHHCCCCCCEEECCCCCCHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHH
EQAANNTGLPVSAFAPALGTESLGDSNFRRVHGVKYAYYAGAMANGISSEELVIALGNAG
HHCCCCCCCCHHHHCCCCCCCCCCCCCCHHHHCCEEHHHHHHHHCCCCCCCEEEEECCCC
ILCSFGAAGLIPSRVEQAINRIQAALPNGPYMFNLIHSPSEPALERGSVELFLKHKVRTV
EEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHCCCEEEEEEHHHEEE
EASAFLGLTPQIVYYRAAGLSRDAQGKIVVANKVIAKVSRTEVAEKFMMPAPAKMLQKLV
EHHHEECCCCCEEEEEECCCCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
DDGSITPEQMELALLVPMADDITAEADSGGHTDNRPLVTLLPTILALKDEIQAKFNYPTP
CCCCCCHHHEEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCC
IRVGCGGGVGTPDAALATFNMGAAYIVTGSVNQACVEAGASDHTRKLLATTEMADVTMAP
EEEECCCCCCCCCHHEEEECCCEEEEEECCCHHHHHHCCCCHHHHHHHHHHHHHHEEECC
AADMFEMGVKLQVVKRGTLFPMRANKLYEIYTRYDSIEAIPADEREKLEKQVFRSSLDEI
CHHHHHCCCEEEEEECCCCCEECCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHH
WAGTVAHFNERDPKQIERAEGNPKRKMALIFRWYLGLSSRWSNSGEVGREMDYQIWAGPA
HHHHHHHCCCCCHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEECCCC
LGAFNQWAKGSYLDNYQDRNAVDVAKHLMYGAAYLSRINSLTAQGVKLPASLLRWKPTQR
CCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCHHC
MA
CC
>Mature Secondary Structure
MNPTTTSEMLSPWPWAVTESNISFDVQVMEQQLKDFSRGCYVVNHSENGVGIAQSAAIVS
CCCCCCHHHCCCCCCEEECCCCCCHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHH
EQAANNTGLPVSAFAPALGTESLGDSNFRRVHGVKYAYYAGAMANGISSEELVIALGNAG
HHCCCCCCCCHHHHCCCCCCCCCCCCCCHHHHCCEEHHHHHHHHCCCCCCCEEEEECCCC
ILCSFGAAGLIPSRVEQAINRIQAALPNGPYMFNLIHSPSEPALERGSVELFLKHKVRTV
EEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHCCCEEEEEEHHHEEE
EASAFLGLTPQIVYYRAAGLSRDAQGKIVVANKVIAKVSRTEVAEKFMMPAPAKMLQKLV
EHHHEECCCCCEEEEEECCCCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
DDGSITPEQMELALLVPMADDITAEADSGGHTDNRPLVTLLPTILALKDEIQAKFNYPTP
CCCCCCHHHEEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCC
IRVGCGGGVGTPDAALATFNMGAAYIVTGSVNQACVEAGASDHTRKLLATTEMADVTMAP
EEEECCCCCCCCCHHEEEECCCEEEEEECCCHHHHHHCCCCHHHHHHHHHHHHHHEEECC
AADMFEMGVKLQVVKRGTLFPMRANKLYEIYTRYDSIEAIPADEREKLEKQVFRSSLDEI
CHHHHHCCCEEEEEECCCCCEECCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHH
WAGTVAHFNERDPKQIERAEGNPKRKMALIFRWYLGLSSRWSNSGEVGREMDYQIWAGPA
HHHHHHHCCCCCHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEECCCC
LGAFNQWAKGSYLDNYQDRNAVDVAKHLMYGAAYLSRINSLTAQGVKLPASLLRWKPTQR
CCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCHHC
MA
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]