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The map label for this gene is proS [H]

Identifier: 157962683

GI number: 157962683

Start: 3497086

End: 3498798

Strand: Direct

Name: proS [H]

Synonym: Spea_2864

Alternate gene names: 157962683

Gene position: 3497086-3498798 (Clockwise)

Preceding gene: 157962681

Following gene: 157962684

Centisome position: 67.58

GC content: 45.53

Gene sequence:

>1713_bases
ATGCGCGTAAGCAAGTACCTGCTATCAACTCAAAAAGAAACTCCTGCAAACGCAGAAGTCGTTAGTCATCAGTTAATGTT
ACGTGCGGGCATGATCCGTCGTAACGCATCAGGCTTATACAGCTGGTTGCCGACAGGTTTGCGTGTACTACGTAAAATCG
AAGCGATTGTTCGTGAAGAAATGAACAAGGCCGGCTCAGTTGAAATTCTTATGCCTATGGTTCAACCAGCTGACCTTTGG
GTTGAAACGGGTCGTTTTGAAAAATTTGGTCCTGAGCTACTTCGTTTCCAAGACCGTCACAACCGTGACTTCGTACTTGG
CCCAACTCACGAAGAAGTGATCACCGATATCGTGCGTAAAGAGGTTAACTCATACAAGCAGTTACCACTTAACCTATACC
AAATTCAAACCAAATTCCGTGACGAAGTTCGTCCACGTTTTGGTGTAATGCGTTCACGTGAATTCTTGATGAAAGATGCA
TATTCTTTCCATATCGATCAAGAAACTATGGATGAAACCTATGAAGCGATGTTCCAAGCTTATAGCAACATTCTTACTCG
CTTAGGCCTAGCTTTCCGTCCAGTTATGGCTGATACGGGTTCTATCGGTGGCAGCATGTCACACGAGTTCCACGTATTGG
CTAACAGTGGTGAAGACTTAATCGCTTACTCAACGGAAAGCGATTACGCGGCAAACATTGAAAAATGTGAAGCGCCTCTG
CCAACTGAAACACGTCAAGCCGCAACCAGCGAAATGACGTTAGTTGACACACCAAACGCTAAGACAATTGCTGAACTTGT
AGAACAGCACGGTATTGCGATTGAGAAAACAGTTAAGACACTAATCGTTAAAGGTGCGACTGAAGAAGCGCCATTAGTGG
CTATCGTTATTCGTGGCGACCACGAGCTAAACGAAGTTAAAGCTGAAAAGTTAGATGCAGTATTAGCACCATTTGAGTTT
GCTGATGAAGCGGCTATCCGTGATGCTATTGGCGCAGGCACAGGTTCTATCGGTCCTGTTGGCCTAAATATGCCAGTATT
TGTTGACCACAGCGTTAGCATCATGAGCGACTTTGGCGCCGGTGCAAACCAAGATGGCAAGCACTACTTCGGCATTAACT
GGGAGCGCGATCTACCAGAAGCACCAGCATTCGATTTACGTAACATCATCGAAGGTGAGCCTAGCCCATGTGGTAAAGGC
ACTATCGCCCTACTTCGCGGTATTGAAGTCGGCCATATCTTCCAGTTAGGTACTAACTACTCTGAAGCGATGAATGCCAA
CGTACTTGACCAAAATGGTAAGTCACAGACTCTACTAATGGGTTGTTACGGTGTGGGTGTAAGCCGTATGGTAGCTGCTG
CAATTGAGCAAAACAATGATGATCGCGGTATTATCTGGCCTGAAGCGATTGCACCGTTCACAGTTGGTATTTTGCCAATG
AACATGCACAAGTCGCATCGCGTTAAAGATATCGCAGAGCAGCTTTATCAAGACCTAAACGATGCAGGTATCGAAGTGAT
GTTCGATGACCGTAAAGAGCGTCCAGGCGTGATGTTTGCTGATATGGAGCTTATCGGTATTCCACACGTTGTGGTTATTG
GTGATCGTAACATCGATAACGGCATGTTCGAATACAAGAACCGTCGTACTGGTGAGAAGCAAGATATTCCACTAGACCAA
ATCGTTGAGTTCTTAAAAGCGCAACAAGCTTAA

Upstream 100 bases:

>100_bases
ACAAAGCCAATAAATTCCACCTAATCGCGAAATCCATAGGTGACAGAACCCCTACAAATGATAAAATAGCGCCCATTCAA
CTCAAATTTGGACACCGGTA

Downstream 100 bases:

>100_bases
TCGTCTGCACACTTAACAGACCGTTCGCTAGAACAATAAAAAACGCCGATGACATCATCGGCGTTTTTTTTGTAAATACA
TCCGCTAAAACTGACTCCCG

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS [H]

Number of amino acids: Translated: 570; Mature: 570

Protein sequence:

>570_residues
MRVSKYLLSTQKETPANAEVVSHQLMLRAGMIRRNASGLYSWLPTGLRVLRKIEAIVREEMNKAGSVEILMPMVQPADLW
VETGRFEKFGPELLRFQDRHNRDFVLGPTHEEVITDIVRKEVNSYKQLPLNLYQIQTKFRDEVRPRFGVMRSREFLMKDA
YSFHIDQETMDETYEAMFQAYSNILTRLGLAFRPVMADTGSIGGSMSHEFHVLANSGEDLIAYSTESDYAANIEKCEAPL
PTETRQAATSEMTLVDTPNAKTIAELVEQHGIAIEKTVKTLIVKGATEEAPLVAIVIRGDHELNEVKAEKLDAVLAPFEF
ADEAAIRDAIGAGTGSIGPVGLNMPVFVDHSVSIMSDFGAGANQDGKHYFGINWERDLPEAPAFDLRNIIEGEPSPCGKG
TIALLRGIEVGHIFQLGTNYSEAMNANVLDQNGKSQTLLMGCYGVGVSRMVAAAIEQNNDDRGIIWPEAIAPFTVGILPM
NMHKSHRVKDIAEQLYQDLNDAGIEVMFDDRKERPGVMFADMELIGIPHVVVIGDRNIDNGMFEYKNRRTGEKQDIPLDQ
IVEFLKAQQA

Sequences:

>Translated_570_residues
MRVSKYLLSTQKETPANAEVVSHQLMLRAGMIRRNASGLYSWLPTGLRVLRKIEAIVREEMNKAGSVEILMPMVQPADLW
VETGRFEKFGPELLRFQDRHNRDFVLGPTHEEVITDIVRKEVNSYKQLPLNLYQIQTKFRDEVRPRFGVMRSREFLMKDA
YSFHIDQETMDETYEAMFQAYSNILTRLGLAFRPVMADTGSIGGSMSHEFHVLANSGEDLIAYSTESDYAANIEKCEAPL
PTETRQAATSEMTLVDTPNAKTIAELVEQHGIAIEKTVKTLIVKGATEEAPLVAIVIRGDHELNEVKAEKLDAVLAPFEF
ADEAAIRDAIGAGTGSIGPVGLNMPVFVDHSVSIMSDFGAGANQDGKHYFGINWERDLPEAPAFDLRNIIEGEPSPCGKG
TIALLRGIEVGHIFQLGTNYSEAMNANVLDQNGKSQTLLMGCYGVGVSRMVAAAIEQNNDDRGIIWPEAIAPFTVGILPM
NMHKSHRVKDIAEQLYQDLNDAGIEVMFDDRKERPGVMFADMELIGIPHVVVIGDRNIDNGMFEYKNRRTGEKQDIPLDQ
IVEFLKAQQA
>Mature_570_residues
MRVSKYLLSTQKETPANAEVVSHQLMLRAGMIRRNASGLYSWLPTGLRVLRKIEAIVREEMNKAGSVEILMPMVQPADLW
VETGRFEKFGPELLRFQDRHNRDFVLGPTHEEVITDIVRKEVNSYKQLPLNLYQIQTKFRDEVRPRFGVMRSREFLMKDA
YSFHIDQETMDETYEAMFQAYSNILTRLGLAFRPVMADTGSIGGSMSHEFHVLANSGEDLIAYSTESDYAANIEKCEAPL
PTETRQAATSEMTLVDTPNAKTIAELVEQHGIAIEKTVKTLIVKGATEEAPLVAIVIRGDHELNEVKAEKLDAVLAPFEF
ADEAAIRDAIGAGTGSIGPVGLNMPVFVDHSVSIMSDFGAGANQDGKHYFGINWERDLPEAPAFDLRNIIEGEPSPCGKG
TIALLRGIEVGHIFQLGTNYSEAMNANVLDQNGKSQTLLMGCYGVGVSRMVAAAIEQNNDDRGIIWPEAIAPFTVGILPM
NMHKSHRVKDIAEQLYQDLNDAGIEVMFDDRKERPGVMFADMELIGIPHVVVIGDRNIDNGMFEYKNRRTGEKQDIPLDQ
IVEFLKAQQA

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI34303926, Length=235, Percent_Identity=42.5531914893617, Blast_Score=206, Evalue=5e-53,
Organism=Escherichia coli, GI1786392, Length=569, Percent_Identity=68.7170474516696, Blast_Score=823, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115532348, Length=264, Percent_Identity=37.5, Blast_Score=175, Evalue=6e-44,
Organism=Caenorhabditis elegans, GI193203271, Length=105, Percent_Identity=35.2380952380952, Blast_Score=75, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6320931, Length=202, Percent_Identity=38.1188118811881, Blast_Score=167, Evalue=5e-42,
Organism=Drosophila melanogaster, GI24656200, Length=234, Percent_Identity=38.8888888888889, Blast_Score=178, Evalue=1e-44,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK [H]

EC number: =6.1.1.15 [H]

Molecular weight: Translated: 63517; Mature: 63517

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVSKYLLSTQKETPANAEVVSHQLMLRAGMIRRNASGLYSWLPTGLRVLRKIEAIVREE
CCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
MNKAGSVEILMPMVQPADLWVETGRFEKFGPELLRFQDRHNRDFVLGPTHEEVITDIVRK
HCCCCCEEEEEECCCCHHHHEECCCCHHCCHHHHHHHHCCCCCEEECCCHHHHHHHHHHH
EVNSYKQLPLNLYQIQTKFRDEVRPRFGVMRSREFLMKDAYSFHIDQETMDETYEAMFQA
HHHHHHCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHHH
YSNILTRLGLAFRPVMADTGSIGGSMSHEFHVLANSGEDLIAYSTESDYAANIEKCEAPL
HHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCEEEEECCCCCCCCHHHCCCCC
PTETRQAATSEMTLVDTPNAKTIAELVEQHGIAIEKTVKTLIVKGATEEAPLVAIVIRGD
CCCHHHHHCCCEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHEECCCCCCCEEEEEEECC
HELNEVKAEKLDAVLAPFEFADEAAIRDAIGAGTGSIGPVGLNMPVFVDHSVSIMSDFGA
CHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCHHHHHHCCC
GANQDGKHYFGINWERDLPEAPAFDLRNIIEGEPSPCGKGTIALLRGIEVGHIFQLGTNY
CCCCCCCEEECCCCCCCCCCCCCHHHHHHHCCCCCCCCCCHHHHHHCCCCCEEEEECCCH
SEAMNANVLDQNGKSQTLLMGCYGVGVSRMVAAAIEQNNDDRGIIWPEAIAPFTVGILPM
HHHCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHCCCCCCCEECCCHHCCCEEEEEEE
NMHKSHRVKDIAEQLYQDLNDAGIEVMFDDRKERPGVMFADMELIGIPHVVVIGDRNIDN
CCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEECEEEECCCEEEEEECCCCCC
GMFEYKNRRTGEKQDIPLDQIVEFLKAQQA
CHHHHCCCCCCCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MRVSKYLLSTQKETPANAEVVSHQLMLRAGMIRRNASGLYSWLPTGLRVLRKIEAIVREE
CCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
MNKAGSVEILMPMVQPADLWVETGRFEKFGPELLRFQDRHNRDFVLGPTHEEVITDIVRK
HCCCCCEEEEEECCCCHHHHEECCCCHHCCHHHHHHHHCCCCCEEECCCHHHHHHHHHHH
EVNSYKQLPLNLYQIQTKFRDEVRPRFGVMRSREFLMKDAYSFHIDQETMDETYEAMFQA
HHHHHHCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHHH
YSNILTRLGLAFRPVMADTGSIGGSMSHEFHVLANSGEDLIAYSTESDYAANIEKCEAPL
HHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCEEEEECCCCCCCCHHHCCCCC
PTETRQAATSEMTLVDTPNAKTIAELVEQHGIAIEKTVKTLIVKGATEEAPLVAIVIRGD
CCCHHHHHCCCEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHEECCCCCCCEEEEEEECC
HELNEVKAEKLDAVLAPFEFADEAAIRDAIGAGTGSIGPVGLNMPVFVDHSVSIMSDFGA
CHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCHHHHHHCCC
GANQDGKHYFGINWERDLPEAPAFDLRNIIEGEPSPCGKGTIALLRGIEVGHIFQLGTNY
CCCCCCCEEECCCCCCCCCCCCCHHHHHHHCCCCCCCCCCHHHHHHCCCCCEEEEECCCH
SEAMNANVLDQNGKSQTLLMGCYGVGVSRMVAAAIEQNNDDRGIIWPEAIAPFTVGILPM
HHHCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHCCCCCCCEECCCHHCCCEEEEEEE
NMHKSHRVKDIAEQLYQDLNDAGIEVMFDDRKERPGVMFADMELIGIPHVVVIGDRNIDN
CCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEECEEEECCCEEEEEECCCCCC
GMFEYKNRRTGEKQDIPLDQIVEFLKAQQA
CHHHHCCCCCCCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA