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The map label for this gene is mdtL [H]

Identifier: 157962589

GI number: 157962589

Start: 3381662

End: 3382861

Strand: Direct

Name: mdtL [H]

Synonym: Spea_2768

Alternate gene names: 157962589

Gene position: 3381662-3382861 (Clockwise)

Preceding gene: 157962588

Following gene: 157962594

Centisome position: 65.35

GC content: 45.58

Gene sequence:

>1200_bases
GTGAGCAGAGTGATGAATTTTAAAGCCATCTTACTGGCCTGTTTAATTATCAGTGTCGGGCAATTGAGTATGGGATTAGT
ATTCCCATCACTGCCTTGGATAGCCAAAGATTTTTCGGTTTCCCATGAACAGGTTCAGATATTGGTCAGTATTTATTTAC
TAGGATTTGGTCCTTCACAGTTTTTCTACGGCCCTATTTCTGATGCGCTTGGACGCAAAAAAGTGTTGTTAATTGGCCTG
ATGATCGCCATGCTAGGTCTACTTATGATCATTTTTCTCAGCCATTCATTTACCGGTATGGTGCTAGGGCGCTTCCTTCA
AGGATTAGGTACCGGATGTTGTGCTGTACTGGCTCGAGCCTCCACACGAGATAACTTCAGTGGTGCAGAACTGCCAGTTG
CAATGTCCTACATAGCAATGGTCGCCTCGATAACACCTCTGTTCGCTCCAGTGCTTGGTGGCTTCATCAACTTCCACTTT
GGTTGGACTATGGTGTTCATCTCTTTACTGGGTTACCTCACTCTCGTTTGGGCTGTTGCGGCATTACGATTCCAGGAAAC
TATCGACCATCCCTCTCCCCTTCCGTCTCCGAAGAAGGTGTTACTTCAGTATCGTGAGCTACTGAGTTCTCGTTACTTCA
TGAGCTTTGCCAGCATAGGTTGGCTAAATTTCAGTTTGATGATCACTACGATTTCGGTGATGCCATTTATCATGCAAAAC
CAAATTGGTATGACGTCTGATAAGTACGCGATGTGGGCGATAATCCCTGCATTAGGCATGTTAATCGGCGCCAGTATCTG
TAATCGGATCCGTCCCTTAATCGGTAGTAAGAAACTCCTGCTATATACGCCAGTGTTGCACCTAGCCGCAGCGGTGTGGT
TGTTCTTCTGCCCAGTCGAGCCTTTATACTTAATGTTGGGACAACTACTGATGATTCTAGGTAACAGTATCGCCTTACCT
TGTGTTCAAGCCATGGTGATGCAGCCATACAAGAAACAAGCGGGAGCGGCGGCAGCGATGTCTGGAGGCGGACAGATGGT
GGTTTCCTCAATAGTCAGTATGGTACTGATAAATTTAGGATTAAGCCAAGCCTGGCAGCTTTCTTTGATCATTGTCGTCT
TTGCAATAATCACGCTCATTAATGTTATGCGTGGTTTTGCCACCCAAGAAGCCATGCAATCAATCACGACTAAACAATAA

Upstream 100 bases:

>100_bases
AACGTGTCAAAGCCAAATTAGGTACATTTGACGATGAAGGCAGCTTGAGACGCAGAGGAAAAGCCGCCTCAACCTCGAAT
GATACTGAGTGAGCAGGATT

Downstream 100 bases:

>100_bases
TACTAAAGAGACCACCCAAGCTGAGGATGTGATGGCAAGAGTTGTCAATAAAGTGAGCCCCGTAAAGCCTGACTCAATGT
AATGGTAGAAAAAAGAGAAA

Product: major facilitator transporter

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 399; Mature: 398

Protein sequence:

>399_residues
MSRVMNFKAILLACLIISVGQLSMGLVFPSLPWIAKDFSVSHEQVQILVSIYLLGFGPSQFFYGPISDALGRKKVLLIGL
MIAMLGLLMIIFLSHSFTGMVLGRFLQGLGTGCCAVLARASTRDNFSGAELPVAMSYIAMVASITPLFAPVLGGFINFHF
GWTMVFISLLGYLTLVWAVAALRFQETIDHPSPLPSPKKVLLQYRELLSSRYFMSFASIGWLNFSLMITTISVMPFIMQN
QIGMTSDKYAMWAIIPALGMLIGASICNRIRPLIGSKKLLLYTPVLHLAAAVWLFFCPVEPLYLMLGQLLMILGNSIALP
CVQAMVMQPYKKQAGAAAAMSGGGQMVVSSIVSMVLINLGLSQAWQLSLIIVVFAIITLINVMRGFATQEAMQSITTKQ

Sequences:

>Translated_399_residues
MSRVMNFKAILLACLIISVGQLSMGLVFPSLPWIAKDFSVSHEQVQILVSIYLLGFGPSQFFYGPISDALGRKKVLLIGL
MIAMLGLLMIIFLSHSFTGMVLGRFLQGLGTGCCAVLARASTRDNFSGAELPVAMSYIAMVASITPLFAPVLGGFINFHF
GWTMVFISLLGYLTLVWAVAALRFQETIDHPSPLPSPKKVLLQYRELLSSRYFMSFASIGWLNFSLMITTISVMPFIMQN
QIGMTSDKYAMWAIIPALGMLIGASICNRIRPLIGSKKLLLYTPVLHLAAAVWLFFCPVEPLYLMLGQLLMILGNSIALP
CVQAMVMQPYKKQAGAAAAMSGGGQMVVSSIVSMVLINLGLSQAWQLSLIIVVFAIITLINVMRGFATQEAMQSITTKQ
>Mature_398_residues
SRVMNFKAILLACLIISVGQLSMGLVFPSLPWIAKDFSVSHEQVQILVSIYLLGFGPSQFFYGPISDALGRKKVLLIGLM
IAMLGLLMIIFLSHSFTGMVLGRFLQGLGTGCCAVLARASTRDNFSGAELPVAMSYIAMVASITPLFAPVLGGFINFHFG
WTMVFISLLGYLTLVWAVAALRFQETIDHPSPLPSPKKVLLQYRELLSSRYFMSFASIGWLNFSLMITTISVMPFIMQNQ
IGMTSDKYAMWAIIPALGMLIGASICNRIRPLIGSKKLLLYTPVLHLAAAVWLFFCPVEPLYLMLGQLLMILGNSIALPC
VQAMVMQPYKKQAGAAAAMSGGGQMVVSSIVSMVLINLGLSQAWQLSLIIVVFAIITLINVMRGFATQEAMQSITTKQ

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. DHA1 family. MdtL (TC 2.A.1.2.22) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790146, Length=374, Percent_Identity=28.0748663101604, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI87082312, Length=365, Percent_Identity=26.027397260274, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1788509, Length=354, Percent_Identity=23.1638418079096, Blast_Score=95, Evalue=7e-21,
Organism=Escherichia coli, GI48994889, Length=342, Percent_Identity=25.7309941520468, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1790794, Length=246, Percent_Identity=26.4227642276423, Blast_Score=83, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 43560; Mature: 43429

Theoretical pI: Translated: 9.90; Mature: 9.90

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
6.5 %Met     (Translated Protein)
8.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
6.3 %Met     (Mature Protein)
7.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SRVMNFKAILLACLIISVGQLSMGLVFPSLPWIAKDFSVSHEQVQILVSIYLLGFGPSQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHHHHHHCCCCC
FFYGPISDALGRKKVLLIGLMIAMLGLLMIIFLSHSFTGMVLGRFLQGLGTGCCAVLARA
CCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCHHHHHHHHHH
STRDNFSGAELPVAMSYIAMVASITPLFAPVLGGFINFHFGWTMVFISLLGYLTLVWAVA
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALRFQETIDHPSPLPSPKKVLLQYRELLSSRYFMSFASIGWLNFSLMITTISVMPFIMQN
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
QIGMTSDKYAMWAIIPALGMLIGASICNRIRPLIGSKKLLLYTPVLHLAAAVWLFFCPVE
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCC
PLYLMLGQLLMILGNSIALPCVQAMVMQPYKKQAGAAAAMSGGGQMVVSSIVSMVLINLG
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHHHC
LSQAWQLSLIIVVFAIITLINVMRGFATQEAMQSITTKQ
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]