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The map label for this gene is pspA [H]

Identifier: 157962490

GI number: 157962490

Start: 3251759

End: 3252436

Strand: Reverse

Name: pspA [H]

Synonym: Spea_2669

Alternate gene names: 157962490

Gene position: 3252436-3251759 (Counterclockwise)

Preceding gene: 157962498

Following gene: 157962489

Centisome position: 62.85

GC content: 43.66

Gene sequence:

>678_bases
ATGGGAATCTTTTCACGCTTTGCAGACATCATTAACTCGAATATCAGCGCATTACTCGATAAGGCTGAAGATCCAGAGAA
AATGGTTCGTCTGATCATCCAAGAGATGGAAGATACGTTAGTTGAAGTGCGCTCTACCTCAGCAAAAGTATTAGCCGAGA
AAAAAGAGATCATTCGTCGTATAGCAAAAGTACAAGAGCAAGTACAAGATTGGGAAAGCAAAGCCGAATTAGCTTTGTCT
AAAGACCGTGAAGATCTTGCTAAGGCCGCGTTAGTTGAAAAGCAAAAAGCCAATGACCTAGCACAAACCCTTGCCGCCGA
GTTGGTTGTAGTTGAAGAGCACATCCTACGCCTTAAAGACGAAGTGAACTTACTGCAAGAGAAGCTTGCCGACGCTAAAG
CGCGCCAAAAGACAATTATCATGCGCAAGCAGACTGCTTCATCGCGTTTAGAGGTGAAAAAGCAGCTTGATTCTAGCAAG
ATTGACAATGCAATGAGCAAGTTTGAGCAATATGAGCGCCGCGTAGAAAGCCTTGAGTCGCAGGTTGATTCTTATGACTT
GGGTAATAAGAAGACGCTTAATGATGAATTTGCAGCCCTAGAAGCTGAAGACTCAGTCAATGCAGAGCTCGAAGCGCTTA
AGGCGAAAATGAAGGGAAGTAAGACTAAAGCTAAGTAA

Upstream 100 bases:

>100_bases
TAATAAATTGTTTAAGTGCCTGTATAGTATTGGAAAGTTAAAGTTGGCACGGAATATGGATTGTAATTATCGAATCCAAC
GTTAATTAGAGGAAAATATT

Downstream 100 bases:

>100_bases
TAAACAAGATCAGAGGTGAGTTATGAACATGGATATTTTGATGGCCCCAATTATTATCTTTTTAATAATCGTGGCTCCGA
TATGGCTAATTCTTCATTAC

Product: phage shock protein A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 225; Mature: 224

Protein sequence:

>225_residues
MGIFSRFADIINSNISALLDKAEDPEKMVRLIIQEMEDTLVEVRSTSAKVLAEKKEIIRRIAKVQEQVQDWESKAELALS
KDREDLAKAALVEKQKANDLAQTLAAELVVVEEHILRLKDEVNLLQEKLADAKARQKTIIMRKQTASSRLEVKKQLDSSK
IDNAMSKFEQYERRVESLESQVDSYDLGNKKTLNDEFAALEAEDSVNAELEALKAKMKGSKTKAK

Sequences:

>Translated_225_residues
MGIFSRFADIINSNISALLDKAEDPEKMVRLIIQEMEDTLVEVRSTSAKVLAEKKEIIRRIAKVQEQVQDWESKAELALS
KDREDLAKAALVEKQKANDLAQTLAAELVVVEEHILRLKDEVNLLQEKLADAKARQKTIIMRKQTASSRLEVKKQLDSSK
IDNAMSKFEQYERRVESLESQVDSYDLGNKKTLNDEFAALEAEDSVNAELEALKAKMKGSKTKAK
>Mature_224_residues
GIFSRFADIINSNISALLDKAEDPEKMVRLIIQEMEDTLVEVRSTSAKVLAEKKEIIRRIAKVQEQVQDWESKAELALSK
DREDLAKAALVEKQKANDLAQTLAAELVVVEEHILRLKDEVNLLQEKLADAKARQKTIIMRKQTASSRLEVKKQLDSSKI
DNAMSKFEQYERRVESLESQVDSYDLGNKKTLNDEFAALEAEDSVNAELEALKAKMKGSKTKAK

Specific function: The phage shock protein (psp) operon (pspABCDE) may play a significant role in the competition for survival under nutrient- or energy-limited conditions. PspA negatively regulates expression of the pspABCDE promoter and of pspG through negative regulation

COG id: COG1842

COG function: function code KT; Phage shock protein A (IM30), suppresses sigma54-dependent transcription

Gene ontology:

Cell location: Cytoplasm. Cell inner membrane; Peripheral membrane protein; Cytoplasmic side (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PspA/IM30 family [H]

Homologues:

Organism=Escherichia coli, GI2367118, Length=218, Percent_Identity=54.5871559633027, Blast_Score=248, Evalue=2e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014319
- InterPro:   IPR007157 [H]

Pfam domain/function: PF04012 PspA_IM30 [H]

EC number: NA

Molecular weight: Translated: 25447; Mature: 25316

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGIFSRFADIINSNISALLDKAEDPEKMVRLIIQEMEDTLVEVRSTSAKVLAEKKEIIRR
CCHHHHHHHHHHCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IAKVQEQVQDWESKAELALSKDREDLAKAALVEKQKANDLAQTLAAELVVVEEHILRLKD
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EVNLLQEKLADAKARQKTIIMRKQTASSRLEVKKQLDSSKIDNAMSKFEQYERRVESLES
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QVDSYDLGNKKTLNDEFAALEAEDSVNAELEALKAKMKGSKTKAK
HHHHCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
GIFSRFADIINSNISALLDKAEDPEKMVRLIIQEMEDTLVEVRSTSAKVLAEKKEIIRR
CHHHHHHHHHHCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IAKVQEQVQDWESKAELALSKDREDLAKAALVEKQKANDLAQTLAAELVVVEEHILRLKD
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EVNLLQEKLADAKARQKTIIMRKQTASSRLEVKKQLDSSKIDNAMSKFEQYERRVESLES
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QVDSYDLGNKKTLNDEFAALEAEDSVNAELEALKAKMKGSKTKAK
HHHHCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]