Click here to switch to the map view.

The map label for this gene is sbcD [H]

Identifier: 157962455

GI number: 157962455

Start: 3207424

End: 3208569

Strand: Reverse

Name: sbcD [H]

Synonym: Spea_2634

Alternate gene names: 157962455

Gene position: 3208569-3207424 (Counterclockwise)

Preceding gene: 157962458

Following gene: 157962454

Centisome position: 62.01

GC content: 47.73

Gene sequence:

>1146_bases
ATGAAATTTATCCATACATCTGACTGGCATATTGGCAGACAGCTTCATAACCAGAGTCTGCTCGATGATCAGTGCTTTGT
TCTCAATCAAATAATCGAACTCGCGACAGAGCATAACGTCGATGCGGTGGTTGTCGCTGGCGATATCTATGACAGATCGG
TTCCGCCAGCGAATGCGGTCGCCCTATTAGATGACGTGGTTAACCGTCTGGTAAACGAGTTAAAAATCCCGCTTATCATG
ATTGCCGGTAATCATGACGGTCACGAACGTCTCGGTTTTGCCTCAAGACAGATGAATGACAGCGGCCTGCATATCGTAGG
GCCGTTAACACAGGAGGTGAGGGCTATTGAGCTTAAAGGTAAACAGGGGAGTGCCATGTTTTATGGTCTACCTTATGCCG
ATCCAGCCACAGTGCGTCAGGTGTTTGATTGTGAGGCTAGCACTCACGAAGAGGCGATGGCAAAACTGCTTGAACAGGTT
GAACTGCACGACAGTAAAGGCTTACCAAAAGTCGTTGTCAGTCACTGTTTTCTCGATGGCGGCAGTGAGTCTGAGTCTGA
GCGTCCGCTGAGTATCGGCGGCGCAGATAAGATCTCACCAAAATTGTTCACACCTTTTAACTATACGGCGTTAGGGCATT
TGCACGGCCCGCAATATAAAGGCGCTGAGCATGTGAGATATTCGGGTTCGCTGCTTAAATACTCCTTCAGTGAACAGCAT
CAGAACAAGTCAGTCACCTTGGTTGAATTAGACTGTGACGGCAAAGCAACCTTTGAGTTATTGCCTTTGAAAGCCGTGCG
AGATGTACGCATTATCGAAGGTGAGTTGGCTCAATTACTCGAACAGGGCAAAACGGATCTGAACCGTGAAGATTACCTGA
TGGTGAGACTCAGTGATAAAACGGCCATCTTAGATGCCATGGGTAAACTGAGAAGTGTGTACCCGAATGTACTGCATCTA
GAGCGGACAGGCTTGATGAGTGACAAGAGTAAGGTTGAGCTAAGCCGAGATCATATTAAGAAGGGCGAATTAGACATGTT
TACTGACTTCTTTGCGCAAGTATCGGGCGAGCCTATGACAGAGGCGCAGCAACAAGCCATGATCACTGCAATCGATGAAT
TACACAAAGCGGAGCGCACATCATGA

Upstream 100 bases:

>100_bases
TCTTACTATCTGCTTTATCAAACGGTAACTTACCGCGCTCAGTCTTAGCAGATAGTCTTAATTGACGCGCAGCCGTCCCC
TCATTGTTAAACGAGAAAGC

Downstream 100 bases:

>100_bases
GACCGCTTACTCTAGAAATGTCTGCGTTCGGCCCATTTGCATCGACGCAAATAACCGACTTTTCAGCGCTAGGCTCTAAT
CCGCTGTTTTTGATCAACGG

Product: nuclease SbcCD subunit D

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 381; Mature: 381

Protein sequence:

>381_residues
MKFIHTSDWHIGRQLHNQSLLDDQCFVLNQIIELATEHNVDAVVVAGDIYDRSVPPANAVALLDDVVNRLVNELKIPLIM
IAGNHDGHERLGFASRQMNDSGLHIVGPLTQEVRAIELKGKQGSAMFYGLPYADPATVRQVFDCEASTHEEAMAKLLEQV
ELHDSKGLPKVVVSHCFLDGGSESESERPLSIGGADKISPKLFTPFNYTALGHLHGPQYKGAEHVRYSGSLLKYSFSEQH
QNKSVTLVELDCDGKATFELLPLKAVRDVRIIEGELAQLLEQGKTDLNREDYLMVRLSDKTAILDAMGKLRSVYPNVLHL
ERTGLMSDKSKVELSRDHIKKGELDMFTDFFAQVSGEPMTEAQQQAMITAIDELHKAERTS

Sequences:

>Translated_381_residues
MKFIHTSDWHIGRQLHNQSLLDDQCFVLNQIIELATEHNVDAVVVAGDIYDRSVPPANAVALLDDVVNRLVNELKIPLIM
IAGNHDGHERLGFASRQMNDSGLHIVGPLTQEVRAIELKGKQGSAMFYGLPYADPATVRQVFDCEASTHEEAMAKLLEQV
ELHDSKGLPKVVVSHCFLDGGSESESERPLSIGGADKISPKLFTPFNYTALGHLHGPQYKGAEHVRYSGSLLKYSFSEQH
QNKSVTLVELDCDGKATFELLPLKAVRDVRIIEGELAQLLEQGKTDLNREDYLMVRLSDKTAILDAMGKLRSVYPNVLHL
ERTGLMSDKSKVELSRDHIKKGELDMFTDFFAQVSGEPMTEAQQQAMITAIDELHKAERTS
>Mature_381_residues
MKFIHTSDWHIGRQLHNQSLLDDQCFVLNQIIELATEHNVDAVVVAGDIYDRSVPPANAVALLDDVVNRLVNELKIPLIM
IAGNHDGHERLGFASRQMNDSGLHIVGPLTQEVRAIELKGKQGSAMFYGLPYADPATVRQVFDCEASTHEEAMAKLLEQV
ELHDSKGLPKVVVSHCFLDGGSESESERPLSIGGADKISPKLFTPFNYTALGHLHGPQYKGAEHVRYSGSLLKYSFSEQH
QNKSVTLVELDCDGKATFELLPLKAVRDVRIIEGELAQLLEQGKTDLNREDYLMVRLSDKTAILDAMGKLRSVYPNVLHL
ERTGLMSDKSKVELSRDHIKKGELDMFTDFFAQVSGEPMTEAQQQAMITAIDELHKAERTS

Specific function: SbcCD cleaves DNA hairpin structures. These structures can inhibit DNA replication and are intermediates in certain DNA recombination reactions. The complex acts as a 3'->5' double strand exonuclease that can open hairpins. It also has a 5' single-strand

COG id: COG0420

COG function: function code L; DNA repair exonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sbcD family [H]

Homologues:

Organism=Escherichia coli, GI1786598, Length=390, Percent_Identity=26.9230769230769, Blast_Score=126, Evalue=3e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004843
- InterPro:   IPR004593 [H]

Pfam domain/function: PF00149 Metallophos [H]

EC number: NA

Molecular weight: Translated: 42396; Mature: 42396

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFIHTSDWHIGRQLHNQSLLDDQCFVLNQIIELATEHNVDAVVVAGDIYDRSVPPANAV
CCCEECCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHH
ALLDDVVNRLVNELKIPLIMIAGNHDGHERLGFASRQMNDSGLHIVGPLTQEVRAIELKG
HHHHHHHHHHHHHHCCCEEEEECCCCCHHHHCHHHHCCCCCCCEEECCCHHCEEEEEEEC
KQGSAMFYGLPYADPATVRQVFDCEASTHEEAMAKLLEQVELHDSKGLPKVVVSHCFLDG
CCCCEEEEECCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCC
GSESESERPLSIGGADKISPKLFTPFNYTALGHLHGPQYKGAEHVRYSGSLLKYSFSEQH
CCCCCCCCCEECCCCCCCCCCEECCCCCEEEECCCCCCCCCCCCEEECCCEEEEEHHHHC
QNKSVTLVELDCDGKATFELLPLKAVRDVRIIEGELAQLLEQGKTDLNREDYLMVRLSDK
CCCCEEEEEEECCCCCEEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCC
TAILDAMGKLRSVYPNVLHLERTGLMSDKSKVELSRDHIKKGELDMFTDFFAQVSGEPMT
HHHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHH
EAQQQAMITAIDELHKAERTS
HHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKFIHTSDWHIGRQLHNQSLLDDQCFVLNQIIELATEHNVDAVVVAGDIYDRSVPPANAV
CCCEECCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHH
ALLDDVVNRLVNELKIPLIMIAGNHDGHERLGFASRQMNDSGLHIVGPLTQEVRAIELKG
HHHHHHHHHHHHHHCCCEEEEECCCCCHHHHCHHHHCCCCCCCEEECCCHHCEEEEEEEC
KQGSAMFYGLPYADPATVRQVFDCEASTHEEAMAKLLEQVELHDSKGLPKVVVSHCFLDG
CCCCEEEEECCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCC
GSESESERPLSIGGADKISPKLFTPFNYTALGHLHGPQYKGAEHVRYSGSLLKYSFSEQH
CCCCCCCCCEECCCCCCCCCCEECCCCCEEEECCCCCCCCCCCCEEECCCEEEEEHHHHC
QNKSVTLVELDCDGKATFELLPLKAVRDVRIIEGELAQLLEQGKTDLNREDYLMVRLSDK
CCCCEEEEEEECCCCCEEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCC
TAILDAMGKLRSVYPNVLHLERTGLMSDKSKVELSRDHIKKGELDMFTDFFAQVSGEPMT
HHHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCHH
EAQQQAMITAIDELHKAERTS
HHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA