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The map label for this gene is tolB [H]

Identifier: 157962385

GI number: 157962385

Start: 3123349

End: 3124671

Strand: Reverse

Name: tolB [H]

Synonym: Spea_2564

Alternate gene names: 157962385

Gene position: 3124671-3123349 (Counterclockwise)

Preceding gene: 157962386

Following gene: 157962384

Centisome position: 60.39

GC content: 46.41

Gene sequence:

>1323_bases
ATGAAAAATTTGGGAAAATGGTTACTGACAAGTCTGCTTATCTGCAGCATGCCAGTAAAGGCCGCATTGGATATTATTAT
TACCGAAGGGGTTGATGCAGCGAGACCCATCGCGGTTATCCCATTTGTTTGGCAAGGCACTGGTCCTATGCCATCGCAGA
TCTCCGATGTGGTGATGTCAGACTTAGCGCGAAGTGGCACGTTTAGCCCAGCCGATGAGTTAAGCCTGCCACAACGCGGT
ATAAGCACTTTGGCACAGTTTAATGCATCGGCTTGGATGGCGCAGCCAGCAGAGGCTGTGGTTATGGGTTCGATTAAGCC
TTATGGTGGCGATAAGTATTTAGTGAGTTTTGAGCTCATCGACTTAGTTAAAGCCCAGCTTCAATCTGATTCTATGTCGA
CTAAAGATTTAGTGATTGATAGTCGTGAAACAGTGATCAGTGCGGCGCAGTTTCGTCAGTACGGTCACCGGATCAGTGAT
GTAGTGTATGAGAAGCTGACGGGTATTCGTGGGGCATTCTTGACTCGCGTTGCTTACGTTGTGGTTAATCACGGTGAAAA
ATCTCCTTATAAACTGATGATTGCCGATTATGATGGTTATAACGAGCAGATGTTGTTACGCTCCCCAGAGCCATTGATGT
CACCATCTTGGTCTCCAGACGGACAGAGCCTAGCTTATGTTAGCTTTGAGAACCGTAAAGCGGAGGTGTTTGTACAGAAC
ATCTATACTCAGCAACGCACTAAGATAACCAGCTTCGATGGTATTAATGGTGCGCCGGTATTTTCACCGGACGGTAAGAA
GTTGGCGGTAACACTTTCGAAGGACGGTCAGCCTGAAGTATATGTAGTGGATATTGCGACTAAGGCGATTAAGCGAGTGA
CGAATCATTACGCTATCGACACTGAGCCGTCTTGGTTCCCTGACGGAAAGTCACTGCTTATTACCTCTGAGCGTGGTGGG
CGGCCACAGTTATATCGCGTCTTCCTGGATTCGGGAAAAATTTCTCGTTTAACGTTTGAAGGTGAATGGAACCTAGGAGG
TTCAATTTCGCCTGATGGACGCAGTATTATTTTTGTTAATCGCATCAATGGTAAATTTAATATCGCTCGCATGGATCTTG
AGACTCGTTTCATGCAAGTCCTGACCAGTACGCGATTAGATGAATCTCCAAGCGTGGCGCCAAATGGCACCATGGTTATT
TATGGAACAACATACCAAGGGAAGCAGGTATTAGCTGCGGTTTCTATGGATGGACGTTTTAAAGCAAGACTGCCAGCTGG
GCAGGGTGAAGTTAAATCACCATCTTGGTCACCATTTCTCTAA

Upstream 100 bases:

>100_bases
TAAAGCGGGCAGGTTACCGGTATCGAGCGAACCAGACGTTTATAACAAGCTAAAAGAAATCAATTTAACAGTTCAACCTG
AGTTTGATTAAAGGAGTCTC

Downstream 100 bases:

>100_bases
CTATAAAGATTAAGGATTTAAAAAATGGATCTGAATAAGCTATTTAAAGCCATGTTAGTCGTACTTCCTATTATGGCTAT
CAGCGCCTGTAGCTCAACTT

Product: translocation protein TolB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 440; Mature: 440

Protein sequence:

>440_residues
MKNLGKWLLTSLLICSMPVKAALDIIITEGVDAARPIAVIPFVWQGTGPMPSQISDVVMSDLARSGTFSPADELSLPQRG
ISTLAQFNASAWMAQPAEAVVMGSIKPYGGDKYLVSFELIDLVKAQLQSDSMSTKDLVIDSRETVISAAQFRQYGHRISD
VVYEKLTGIRGAFLTRVAYVVVNHGEKSPYKLMIADYDGYNEQMLLRSPEPLMSPSWSPDGQSLAYVSFENRKAEVFVQN
IYTQQRTKITSFDGINGAPVFSPDGKKLAVTLSKDGQPEVYVVDIATKAIKRVTNHYAIDTEPSWFPDGKSLLITSERGG
RPQLYRVFLDSGKISRLTFEGEWNLGGSISPDGRSIIFVNRINGKFNIARMDLETRFMQVLTSTRLDESPSVAPNGTMVI
YGTTYQGKQVLAAVSMDGRFKARLPAGQGEVKSPSWSPFL

Sequences:

>Translated_440_residues
MKNLGKWLLTSLLICSMPVKAALDIIITEGVDAARPIAVIPFVWQGTGPMPSQISDVVMSDLARSGTFSPADELSLPQRG
ISTLAQFNASAWMAQPAEAVVMGSIKPYGGDKYLVSFELIDLVKAQLQSDSMSTKDLVIDSRETVISAAQFRQYGHRISD
VVYEKLTGIRGAFLTRVAYVVVNHGEKSPYKLMIADYDGYNEQMLLRSPEPLMSPSWSPDGQSLAYVSFENRKAEVFVQN
IYTQQRTKITSFDGINGAPVFSPDGKKLAVTLSKDGQPEVYVVDIATKAIKRVTNHYAIDTEPSWFPDGKSLLITSERGG
RPQLYRVFLDSGKISRLTFEGEWNLGGSISPDGRSIIFVNRINGKFNIARMDLETRFMQVLTSTRLDESPSVAPNGTMVI
YGTTYQGKQVLAAVSMDGRFKARLPAGQGEVKSPSWSPFL
>Mature_440_residues
MKNLGKWLLTSLLICSMPVKAALDIIITEGVDAARPIAVIPFVWQGTGPMPSQISDVVMSDLARSGTFSPADELSLPQRG
ISTLAQFNASAWMAQPAEAVVMGSIKPYGGDKYLVSFELIDLVKAQLQSDSMSTKDLVIDSRETVISAAQFRQYGHRISD
VVYEKLTGIRGAFLTRVAYVVVNHGEKSPYKLMIADYDGYNEQMLLRSPEPLMSPSWSPDGQSLAYVSFENRKAEVFVQN
IYTQQRTKITSFDGINGAPVFSPDGKKLAVTLSKDGQPEVYVVDIATKAIKRVTNHYAIDTEPSWFPDGKSLLITSERGG
RPQLYRVFLDSGKISRLTFEGEWNLGGSISPDGRSIIFVNRINGKFNIARMDLETRFMQVLTSTRLDESPSVAPNGTMVI
YGTTYQGKQVLAAVSMDGRFKARLPAGQGEVKSPSWSPFL

Specific function: Involved in the tonB-independent uptake of proteins [H]

COG id: COG0823

COG function: function code U; Periplasmic component of the Tol biopolymer transport system

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tolB family [H]

Homologues:

Organism=Escherichia coli, GI1786961, Length=429, Percent_Identity=44.7552447552448, Blast_Score=377, Evalue=1e-106,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011659
- InterPro:   IPR014167
- InterPro:   IPR007195 [H]

Pfam domain/function: PF07676 PD40; PF04052 TolB_N [H]

EC number: NA

Molecular weight: Translated: 48422; Mature: 48422

Theoretical pI: Translated: 8.73; Mature: 8.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNLGKWLLTSLLICSMPVKAALDIIITEGVDAARPIAVIPFVWQGTGPMPSQISDVVMS
CCCHHHHHHHHHHHHCCCHHHEEEEEEECCCCCCCCEEEEEEEECCCCCCHHHHHHHHHH
DLARSGTFSPADELSLPQRGISTLAQFNASAWMAQPAEAVVMGSIKPYGGDKYLVSFELI
HHHHCCCCCCHHHCCCCHHHHHHHHHCCCCHHCCCCCCEEEEECCCCCCCCEEEEEHHHH
DLVKAQLQSDSMSTKDLVIDSRETVISAAQFRQYGHRISDVVYEKLTGIRGAFLTRVAYV
HHHHHHHHCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHEEEE
VVNHGEKSPYKLMIADYDGYNEQMLLRSPEPLMSPSWSPDGQSLAYVSFENRKAEVFVQN
EEECCCCCCEEEEEEECCCCCCEEEEECCCCCCCCCCCCCCCEEEEEEECCCCEEEHHHH
IYTQQRTKITSFDGINGAPVFSPDGKKLAVTLSKDGQPEVYVVDIATKAIKRVTNHYAID
HHHHHHCEEEECCCCCCCCEECCCCCEEEEEECCCCCCCEEEEEHHHHHHHHHHHCEEEC
TEPSWFPDGKSLLITSERGGRPQLYRVFLDSGKISRLTFEGEWNLGGSISPDGRSIIFVN
CCCCCCCCCCEEEEECCCCCCCEEEEEEECCCCEEEEEEECCCCCCCCCCCCCCEEEEEE
RINGKFNIARMDLETRFMQVLTSTRLDESPSVAPNGTMVIYGTTYQGKQVLAAVSMDGRF
ECCCEEEEEEECHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCEEEEEEECCCEE
KARLPAGQGEVKSPSWSPFL
EEECCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKNLGKWLLTSLLICSMPVKAALDIIITEGVDAARPIAVIPFVWQGTGPMPSQISDVVMS
CCCHHHHHHHHHHHHCCCHHHEEEEEEECCCCCCCCEEEEEEEECCCCCCHHHHHHHHHH
DLARSGTFSPADELSLPQRGISTLAQFNASAWMAQPAEAVVMGSIKPYGGDKYLVSFELI
HHHHCCCCCCHHHCCCCHHHHHHHHHCCCCHHCCCCCCEEEEECCCCCCCCEEEEEHHHH
DLVKAQLQSDSMSTKDLVIDSRETVISAAQFRQYGHRISDVVYEKLTGIRGAFLTRVAYV
HHHHHHHHCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHEEEE
VVNHGEKSPYKLMIADYDGYNEQMLLRSPEPLMSPSWSPDGQSLAYVSFENRKAEVFVQN
EEECCCCCCEEEEEEECCCCCCEEEEECCCCCCCCCCCCCCCEEEEEEECCCCEEEHHHH
IYTQQRTKITSFDGINGAPVFSPDGKKLAVTLSKDGQPEVYVVDIATKAIKRVTNHYAID
HHHHHHCEEEECCCCCCCCEECCCCCEEEEEECCCCCCCEEEEEHHHHHHHHHHHCEEEC
TEPSWFPDGKSLLITSERGGRPQLYRVFLDSGKISRLTFEGEWNLGGSISPDGRSIIFVN
CCCCCCCCCCEEEEECCCCCCCEEEEEEECCCCEEEEEEECCCCCCCCCCCCCCEEEEEE
RINGKFNIARMDLETRFMQVLTSTRLDESPSVAPNGTMVIYGTTYQGKQVLAAVSMDGRF
ECCCEEEEEEECHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCEEEEEEECCCEE
KARLPAGQGEVKSPSWSPFL
EEECCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA