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The map label for this gene is 157962328

Identifier: 157962328

GI number: 157962328

Start: 3056014

End: 3056853

Strand: Direct

Name: 157962328

Synonym: Spea_2507

Alternate gene names: NA

Gene position: 3056014-3056853 (Clockwise)

Preceding gene: 157962326

Following gene: 157962329

Centisome position: 59.06

GC content: 42.26

Gene sequence:

>840_bases
ATGACATTAGAAACAATCGATTATCGAGCCACGGATGCCGCTGAGAAATTTGTAGCTTCACTGCGTGAAACAGGCTTTGG
CGTATTAACTAACCATCCTATTGACCAGAAATTGGTTGAGACCATTTATCAAGAGTGGCACGCGTTTTTTAATACCGAAG
AAAAACGTGCATTCCTGTTTAAGCCTGAAACACAAGATGGCTTCTTCCCTGCTGAAATCTCAGAAACAGCAAAAGGGCAT
AGCGTTAAAGACATCAAAGAATACTTTCACGTTTATCCTTGGGGACGTATTCCAGAATCTTTAAAAGACAATATTTTAAA
GTATTACAATAATGCTAACTCATTAGCCTCTGAGTTATTGCAATGGGTTGAAGAGCATTCTCCAGCTGAAGTAAAACAGA
AGTTTTCAATCGAACTACCAAAAATGATTCAAGATAGTCAGCGCACTTTGCTTCGTGTACTGCATTATCCACCAATGTCC
GGCGATGAAGAGTTAGGCGCGATTCGAGCCGCAGCTCATGAAGATATCAACCTATTAACCGTACTTCCCGCTGCAAACGA
GCCCGGACTTCAAGTACAGTTACAGGACGGAAACTGGATAGATGTCCCTAGCGACTTTGGCAATTTGATCATCAATATTG
GTGATATGTTGCAAGAGGCCTCTGGCGGTTACTTTCCATCGACGAGTCACCGTGTCATTAACCCTAAAGGCATGGACAAG
ACAAAGTCTCGCCTGTCGTTACCACTTTTCTTACACCCAAACCCTGAAGTCAAATTGTCAGATAAATACACTGCCGACAG
CTACCTGATGGAACGATTAAGAGAATTAGGGGTAATTTAG

Upstream 100 bases:

>100_bases
TTGTATAAACAATCAGAAGCGTGATCTAGCTCACTCACGTTTGATTCAGCAGTTTCAGCTCGTTAAACTTACCGCGGGTT
TCATAATAAGAAGAATCAAG

Downstream 100 bases:

>100_bases
CCTCATTCACACTCAATAAGAGCTCAATAAGCGCCTTAGGGCGCTTTTTGTTTGTTAAACTTAAGCAGAACAAGGTAAAA
TCCCCTTAATTTAGTAATTT

Product: 2OG-Fe(II) oxygenase

Products: isopenicillin N; H2O

Alternate protein names: Oxidoreductase; 2OG-Fe(II) Oxygenase Family Oxidoreductase; Oxidoreductase 2OG-Fe(II) Oxygenase Family; Isopenicillin N Synthase; Iron/Ascorbate Family Oxidoreductase; 2OG-Fe(II) Oxygenase Superfamily Protein; Flavonol Synthase/Dioxygenase; Iron/Ascorbate Oxidoreductase Family Protein; Oxidoreductase 2OG-Fe(II) Oxygenase Family Protein; 2og-Fe(Ii) Oxygenase; Oxidoreductase Iron/Ascorbate Family Protein; Iron/Ascorbate Oxidoreductase; 1-Aminocyclopropane-1-Carboxylate Oxidase; Oxygenase; Isopenicillin N Synthase And Related Dioxygenases; Oxidoreductase 2og-Fe(Ii) Oxygenase Family; Iron/Ascorbate-Dependent Oxidoreductase Family Protein; Isopenicillin N Synthetase; Iron/Ascorbate-Dependent Oxidoreductase; Oxidoreductase Iron/Ascorbate Family; 2OG-Fe(II) Oxygenase Family Protein; Fe-Containing Oxidoreductase; Isopenicillin-N Synthase; 2-Oxobutyrate Oxidase; Flavonol Synthase/Flavanone 3-Hydroxylase; 2-Oxoglutarate And Fe(Ii)-Dependent Oxygenase; Dioxygenase; Oxygenase/Oxidoreductase

Number of amino acids: Translated: 279; Mature: 278

Protein sequence:

>279_residues
MTLETIDYRATDAAEKFVASLRETGFGVLTNHPIDQKLVETIYQEWHAFFNTEEKRAFLFKPETQDGFFPAEISETAKGH
SVKDIKEYFHVYPWGRIPESLKDNILKYYNNANSLASELLQWVEEHSPAEVKQKFSIELPKMIQDSQRTLLRVLHYPPMS
GDEELGAIRAAAHEDINLLTVLPAANEPGLQVQLQDGNWIDVPSDFGNLIINIGDMLQEASGGYFPSTSHRVINPKGMDK
TKSRLSLPLFLHPNPEVKLSDKYTADSYLMERLRELGVI

Sequences:

>Translated_279_residues
MTLETIDYRATDAAEKFVASLRETGFGVLTNHPIDQKLVETIYQEWHAFFNTEEKRAFLFKPETQDGFFPAEISETAKGH
SVKDIKEYFHVYPWGRIPESLKDNILKYYNNANSLASELLQWVEEHSPAEVKQKFSIELPKMIQDSQRTLLRVLHYPPMS
GDEELGAIRAAAHEDINLLTVLPAANEPGLQVQLQDGNWIDVPSDFGNLIINIGDMLQEASGGYFPSTSHRVINPKGMDK
TKSRLSLPLFLHPNPEVKLSDKYTADSYLMERLRELGVI
>Mature_278_residues
TLETIDYRATDAAEKFVASLRETGFGVLTNHPIDQKLVETIYQEWHAFFNTEEKRAFLFKPETQDGFFPAEISETAKGHS
VKDIKEYFHVYPWGRIPESLKDNILKYYNNANSLASELLQWVEEHSPAEVKQKFSIELPKMIQDSQRTLLRVLHYPPMSG
DEELGAIRAAAHEDINLLTVLPAANEPGLQVQLQDGNWIDVPSDFGNLIINIGDMLQEASGGYFPSTSHRVINPKGMDKT
KSRLSLPLFLHPNPEVKLSDKYTADSYLMERLRELGVI

Specific function: Unknown

COG id: COG3491

COG function: function code R; Isopenicillin N synthase and related dioxygenases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Drosophila melanogaster, GI85816264, Length=264, Percent_Identity=25, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI28572124, Length=265, Percent_Identity=24.9056603773585, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 1.21.3.1

Molecular weight: Translated: 31735; Mature: 31604

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLETIDYRATDAAEKFVASLRETGFGVLTNHPIDQKLVETIYQEWHAFFNTEEKRAFLF
CCCCCCCCCCHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHCCCCCEEEEE
KPETQDGFFPAEISETAKGHSVKDIKEYFHVYPWGRIPESLKDNILKYYNNANSLASELL
CCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCHHHHHHHHH
QWVEEHSPAEVKQKFSIELPKMIQDSQRTLLRVLHYPPMSGDEELGAIRAAAHEDINLLT
HHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEEE
VLPAANEPGLQVQLQDGNWIDVPSDFGNLIINIGDMLQEASGGYFPSTSHRVINPKGMDK
EEECCCCCCCEEEECCCCEEECCHHHHHHEEHHHHHHHHHCCCCCCCCCCEEECCCCCCH
TKSRLSLPLFLHPNPEVKLSDKYTADSYLMERLRELGVI
HHHHCCCEEEECCCCCEEECCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TLETIDYRATDAAEKFVASLRETGFGVLTNHPIDQKLVETIYQEWHAFFNTEEKRAFLF
CCCCCCCCCHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHCCCCCEEEEE
KPETQDGFFPAEISETAKGHSVKDIKEYFHVYPWGRIPESLKDNILKYYNNANSLASELL
CCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCHHHHHHHHH
QWVEEHSPAEVKQKFSIELPKMIQDSQRTLLRVLHYPPMSGDEELGAIRAAAHEDINLLT
HHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEEE
VLPAANEPGLQVQLQDGNWIDVPSDFGNLIINIGDMLQEASGGYFPSTSHRVINPKGMDK
EEECCCCCCCEEEECCCCEEECCHHHHHHEEHHHHHHHHHCCCCCCCCCCEEECCCCCCH
TKSRLSLPLFLHPNPEVKLSDKYTADSYLMERLRELGVI
HHHHCCCEEEECCCCCEEECCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine; O2

Specific reaction: N-[(5S)-5-amino-5-carboxypentanoyl]-L-cysteinyl-D-valine + O2 = isopenicillin N + 2 H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA