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The map label for this gene is slt [H]

Identifier: 157962236

GI number: 157962236

Start: 2944618

End: 2946549

Strand: Direct

Name: slt [H]

Synonym: Spea_2415

Alternate gene names: 157962236

Gene position: 2944618-2946549 (Clockwise)

Preceding gene: 157962235

Following gene: 157962237

Centisome position: 56.91

GC content: 44.88

Gene sequence:

>1932_bases
ATGCACAAGCAGTTGAGTCGCATTTTAATCGGGGTAATCGCAGTATTTGCATCCTCCTCTGCTGTTGCTTCCTCGTTTAC
TCAAGAGCAGCAGCAATATTTAGACGCCAGAACCGCACTAAAGAAAAAGCAATTAGATCGTTACCAATCTCTTAGGGGTG
AGCTGGATGATTATCCTCTCACCATCTACCTTGATTTTCATAACAATGTTAACGACATTGTAAATCTTCCCGCGAAACAG
GCCAAACAAGCATTTGCTGTTTTTGAGGGCAGCCCGCTGTATAACACCGCTCGCTACCGCTACCTAAAGCGCAGTGGCAG
TGAAAAACGCTGGAAGGACTTCCTAGCCGTCAGTCCAGACAAGCCAAATAATGTCGCCCTACAGTGTTACTTTTACCGTG
CACAATTACAGCAAGGTAATCAACAACTCGCTTTTGATGGCGCCGAATCACTATGGCTTTATGGGCGTTCTCGACCAAAA
GAGTGCGACCCGTTATTTAAAGAGTGGCAAAAAGCTGGCCTTCGGACCCAAGAGTTAATTTGGTCACGTATGCTACTCAG
TTTCGAGCAGGGACAATATGGACTGCTCTCTTACCTTTCTCGCCAATTGACCAGCAATAAAGGCGATGCGAAACGATTAC
TCGCGGTGTATAAAGATCCCCGCAGTCTTCGTCATAAATCAAAATTCAGCGGCTCGGCAAAAATCAATGCCACGATTGTG
GATCTGGGTTTAAGAAAATTAGCTAAGAAAGATTTAAAACGAGCAGTCAAACTGTTTGCCACTTATCAAAAGGCAGACCG
TTTCAGTGATTACCAAGGAAGAAAACTCAGTCGCTACCTAGTCAGAAGAGCGCTGATTTATCAAGTACCCGAGTTGCAAA
GCTATGTTGATACCATTTTGCCCCTGCTCGATAGCGATGACTTAGTCGAAATGCGTCTACGCTGGGCCCTGAGAGATAAT
GATCAAAAAAGTTTTAACCAATTCTTACCCCAGCTAAGCGAGCAAAAACAAAATACAGCCCGCTGGCAATACTGGCGAGC
CTCTGTTTTGGCTGATGAAAATACTGACACAAGCCTACAGCAATCAAAAGCATTGTTTGAAAAGCTCAGTCACAAACGTA
ATTTTTATGGTTATCTAGCCGCGGCTAAACTTAAGCAGCCCTACAAGTTGCAGCACCAAGCGACGAAAAGCGATAAAGGC
TTACAACCTAGTTTATTCACAGACAAAGGACTTGCCCGAGTACAGGAATTACTCGCTATCGACAAACAATCGGATGCCAG
AGCCGAATGGGTGATGTTGCTTAATCGGCACAATAAGCAGATGCAAAAAGAGTATGCGGTCTATGCCGTTGAGCAAAAGT
GGCACAGTTTAGGGGTTCAGGCCAGCATACAAGCTCAGCTTTGGAACGACATGGATATGCGTTTTCCTTATGCTGCACAA
GAGTCTTTTAGTCGAGCCAGTAAGAAGTACGCCGTGGATATCGATGAGATCCGCGCTATTTCTCGCCGAGAAAGTGCCTA
CTACCCCAATGCAACATCGGGAGTCGGAGCTAGAGGGTATATGCAGTTAATGCCAGCGACGGCAAAACAAACAGCCAAGA
AAGCCAAACTGCCTTATAAAGGCACTAAAAGCCTATACGATGCCAAGCTCAATATCGCTTTAGGCAGCGCCTATTACGGT
GGTTTACTCAAGCAATTTGATCATAATCGAGTTCTGGCTACCGCTTCTTATAATGCCGGGCCTCATCGTATAAATCGCTG
GTTAAAAAGAACTGACGGTAAACTCGATGTGGTGAGTTTTATCGAGTCAATTCCCTACCGAGAAACAAGGGAATACGTGC
AAGCGGTATTGAGCTATCGAGTTATTTACCAAGTTAAACAAGGAAAAGCACCTGAGCTACTCTCAGAGCAAGAGTTAGAA
TTTAACTATTAA

Upstream 100 bases:

>100_bases
TAAAGCGCTAAGCTTATCTGGTGATGATTTTCATCATGATGAATTGACTTTAGATACCGAAAAAATTTCTTAATAGGGAC
TTTTAGGAGGATAGTTACCA

Downstream 100 bases:

>100_bases
CCTCATAAGTTCAGCGAGCCATAACGACTGCCCCAATCGTTCTGGCTCGTAGTGACAATAGCCATACATTTGGTCGTACT
GATGCGAAGTTATCAGATAG

Product: lytic transglycosylase

Products: 1,6-Anhydrobond [C]

Alternate protein names: Exomuramidase; Peptidoglycan lytic exotransglycosylase; Slt70 [H]

Number of amino acids: Translated: 643; Mature: 643

Protein sequence:

>643_residues
MHKQLSRILIGVIAVFASSSAVASSFTQEQQQYLDARTALKKKQLDRYQSLRGELDDYPLTIYLDFHNNVNDIVNLPAKQ
AKQAFAVFEGSPLYNTARYRYLKRSGSEKRWKDFLAVSPDKPNNVALQCYFYRAQLQQGNQQLAFDGAESLWLYGRSRPK
ECDPLFKEWQKAGLRTQELIWSRMLLSFEQGQYGLLSYLSRQLTSNKGDAKRLLAVYKDPRSLRHKSKFSGSAKINATIV
DLGLRKLAKKDLKRAVKLFATYQKADRFSDYQGRKLSRYLVRRALIYQVPELQSYVDTILPLLDSDDLVEMRLRWALRDN
DQKSFNQFLPQLSEQKQNTARWQYWRASVLADENTDTSLQQSKALFEKLSHKRNFYGYLAAAKLKQPYKLQHQATKSDKG
LQPSLFTDKGLARVQELLAIDKQSDARAEWVMLLNRHNKQMQKEYAVYAVEQKWHSLGVQASIQAQLWNDMDMRFPYAAQ
ESFSRASKKYAVDIDEIRAISRRESAYYPNATSGVGARGYMQLMPATAKQTAKKAKLPYKGTKSLYDAKLNIALGSAYYG
GLLKQFDHNRVLATASYNAGPHRINRWLKRTDGKLDVVSFIESIPYRETREYVQAVLSYRVIYQVKQGKAPELLSEQELE
FNY

Sequences:

>Translated_643_residues
MHKQLSRILIGVIAVFASSSAVASSFTQEQQQYLDARTALKKKQLDRYQSLRGELDDYPLTIYLDFHNNVNDIVNLPAKQ
AKQAFAVFEGSPLYNTARYRYLKRSGSEKRWKDFLAVSPDKPNNVALQCYFYRAQLQQGNQQLAFDGAESLWLYGRSRPK
ECDPLFKEWQKAGLRTQELIWSRMLLSFEQGQYGLLSYLSRQLTSNKGDAKRLLAVYKDPRSLRHKSKFSGSAKINATIV
DLGLRKLAKKDLKRAVKLFATYQKADRFSDYQGRKLSRYLVRRALIYQVPELQSYVDTILPLLDSDDLVEMRLRWALRDN
DQKSFNQFLPQLSEQKQNTARWQYWRASVLADENTDTSLQQSKALFEKLSHKRNFYGYLAAAKLKQPYKLQHQATKSDKG
LQPSLFTDKGLARVQELLAIDKQSDARAEWVMLLNRHNKQMQKEYAVYAVEQKWHSLGVQASIQAQLWNDMDMRFPYAAQ
ESFSRASKKYAVDIDEIRAISRRESAYYPNATSGVGARGYMQLMPATAKQTAKKAKLPYKGTKSLYDAKLNIALGSAYYG
GLLKQFDHNRVLATASYNAGPHRINRWLKRTDGKLDVVSFIESIPYRETREYVQAVLSYRVIYQVKQGKAPELLSEQELE
FNY
>Mature_643_residues
MHKQLSRILIGVIAVFASSSAVASSFTQEQQQYLDARTALKKKQLDRYQSLRGELDDYPLTIYLDFHNNVNDIVNLPAKQ
AKQAFAVFEGSPLYNTARYRYLKRSGSEKRWKDFLAVSPDKPNNVALQCYFYRAQLQQGNQQLAFDGAESLWLYGRSRPK
ECDPLFKEWQKAGLRTQELIWSRMLLSFEQGQYGLLSYLSRQLTSNKGDAKRLLAVYKDPRSLRHKSKFSGSAKINATIV
DLGLRKLAKKDLKRAVKLFATYQKADRFSDYQGRKLSRYLVRRALIYQVPELQSYVDTILPLLDSDDLVEMRLRWALRDN
DQKSFNQFLPQLSEQKQNTARWQYWRASVLADENTDTSLQQSKALFEKLSHKRNFYGYLAAAKLKQPYKLQHQATKSDKG
LQPSLFTDKGLARVQELLAIDKQSDARAEWVMLLNRHNKQMQKEYAVYAVEQKWHSLGVQASIQAQLWNDMDMRFPYAAQ
ESFSRASKKYAVDIDEIRAISRRESAYYPNATSGVGARGYMQLMPATAKQTAKKAKLPYKGTKSLYDAKLNIALGSAYYG
GLLKQFDHNRVLATASYNAGPHRINRWLKRTDGKLDVVSFIESIPYRETREYVQAVLSYRVIYQVKQGKAPELLSEQELE
FNY

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasm. Note=Tightly associated with the murein sacculus (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=631, Percent_Identity=30.2694136291601, Blast_Score=335, Evalue=4e-93,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016026
- InterPro:   IPR008258
- InterPro:   IPR012289
- InterPro:   IPR008939
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 74277; Mature: 74277

Theoretical pI: Translated: 10.22; Mature: 10.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHKQLSRILIGVIAVFASSSAVASSFTQEQQQYLDARTALKKKQLDRYQSLRGELDDYPL
CCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCE
TIYLDFHNNVNDIVNLPAKQAKQAFAVFEGSPLYNTARYRYLKRSGSEKRWKDFLAVSPD
EEEEECCCCCHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHCEEECCC
KPNNVALQCYFYRAQLQQGNQQLAFDGAESLWLYGRSRPKECDPLFKEWQKAGLRTQELI
CCCCEEEEEEEEHHHHHCCCCEEEECCCCCEEEECCCCCCCCCHHHHHHHHHCCCHHHHH
WSRMLLSFEQGQYGLLSYLSRQLTSNKGDAKRLLAVYKDPRSLRHKSKFSGSAKINATIV
HHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHCCCCCEEEEHHH
DLGLRKLAKKDLKRAVKLFATYQKADRFSDYQGRKLSRYLVRRALIYQVPELQSYVDTIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHH
PLLDSDDLVEMRLRWALRDNDQKSFNQFLPQLSEQKQNTARWQYWRASVLADENTDTSLQ
HHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHH
QSKALFEKLSHKRNFYGYLAAAKLKQPYKLQHQATKSDKGLQPSLFTDKGLARVQELLAI
HHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHH
DKQSDARAEWVMLLNRHNKQMQKEYAVYAVEQKWHSLGVQASIQAQLWNDMDMRFPYAAQ
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHCCCCCCCCCHHH
ESFSRASKKYAVDIDEIRAISRRESAYYPNATSGVGARGYMQLMPATAKQTAKKAKLPYK
HHHHHHHHCEECCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHCCCCCC
GTKSLYDAKLNIALGSAYYGGLLKQFDHNRVLATASYNAGPHRINRWLKRTDGKLDVVSF
CCHHHHHCEEEEEECCHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHCCCCHHHHHH
IESIPYRETREYVQAVLSYRVIYQVKQGKAPELLSEQELEFNY
HHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCC
>Mature Secondary Structure
MHKQLSRILIGVIAVFASSSAVASSFTQEQQQYLDARTALKKKQLDRYQSLRGELDDYPL
CCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCE
TIYLDFHNNVNDIVNLPAKQAKQAFAVFEGSPLYNTARYRYLKRSGSEKRWKDFLAVSPD
EEEEECCCCCHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHCEEECCC
KPNNVALQCYFYRAQLQQGNQQLAFDGAESLWLYGRSRPKECDPLFKEWQKAGLRTQELI
CCCCEEEEEEEEHHHHHCCCCEEEECCCCCEEEECCCCCCCCCHHHHHHHHHCCCHHHHH
WSRMLLSFEQGQYGLLSYLSRQLTSNKGDAKRLLAVYKDPRSLRHKSKFSGSAKINATIV
HHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHCCCCCEEEEHHH
DLGLRKLAKKDLKRAVKLFATYQKADRFSDYQGRKLSRYLVRRALIYQVPELQSYVDTIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHH
PLLDSDDLVEMRLRWALRDNDQKSFNQFLPQLSEQKQNTARWQYWRASVLADENTDTSLQ
HHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHH
QSKALFEKLSHKRNFYGYLAAAKLKQPYKLQHQATKSDKGLQPSLFTDKGLARVQELLAI
HHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHH
DKQSDARAEWVMLLNRHNKQMQKEYAVYAVEQKWHSLGVQASIQAQLWNDMDMRFPYAAQ
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHCCCCCCCCCHHH
ESFSRASKKYAVDIDEIRAISRRESAYYPNATSGVGARGYMQLMPATAKQTAKKAKLPYK
HHHHHHHHCEECCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHCCCCCC
GTKSLYDAKLNIALGSAYYGGLLKQFDHNRVLATASYNAGPHRINRWLKRTDGKLDVVSF
CCHHHHHCEEEEEECCHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHCCCCHHHHHH
IESIPYRETREYVQAVLSYRVIYQVKQGKAPELLSEQELEFNY
HHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]