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The map label for this gene is bepF [H]

Identifier: 157962013

GI number: 157962013

Start: 2658015

End: 2659241

Strand: Direct

Name: bepF [H]

Synonym: Spea_2192

Alternate gene names: 157962013

Gene position: 2658015-2659241 (Clockwise)

Preceding gene: 157962010

Following gene: 157962014

Centisome position: 51.37

GC content: 45.31

Gene sequence:

>1227_bases
ATGCCAAGTCATCTCAACAGCACAGCATTCAAATTAACACTGCTTTTTTCATCAGTGGCACTCATTACTGGCTGCGAAAA
AGAACCAGTGCAAATCACCCCTAAATTAGTTGTTGTTGAAAAGGTATCGACAGCAACTGTGCCACTTTATGGCAATTATG
TCGGAGTAACAAAGGCCTCGTTGGATGTTGAAGTAAGAGCGAGGGTCGATGGCTTTGTTGAAAACAAAACTTTTATCGAA
GGCAGTGCCGTCAAAGAAGGGGCACTACTTTATCGTATCGATAATCGGCCTTATGTCGCTGTGGTTAATCGTTTAAGTGC
TAATGTTGAATCCCAGCAATCAGCACTTGAAAAGGCGCAACGTGATGTCGAACGTTTAAAGCCCTTGTATGAGCAAGACG
CTGCCAGTCAGTTAGATTTTGATAATGCGCTGTCGGTTCTATCGCAAGCAAAATCCAGTGTTGCAGCAAGTAAAGCCGAA
CTTGAAGAGGCAAGGCTTGAACTCAGTTATACCGAGATTCAGTCTCCCATTAGTGGATTAGTCAGCCGCTCAGAAGTTGA
TATCGGCGCATTGGTCGGTAGCAGCGGTCAATCGTTATTAACTCGGGTTAAGCAGGTCGACCCTATTTATGTCACCTTTA
ATATGTCGGCGTTAGATTATCTTAATGCGCGCCGACGGATGACCAGTGACAAAGAACAACAAGCCGCTGAATCAGAAGGT
AAAGCCGTTGAAGGTTTCGTTACAATTACATTGCCAGATAACAGTGAGTACCGGTATTTAGGTGATGTCGGCTTTACCGA
TCCTTCGGTCAACCCTGAAACTGGGACCTTTCAGGTAAGGGCTGAACTCCCCAACCCAGATAAAGAGTTACTGCCGGGCC
AATACACCAATGTCAGAATTAAGCTCAGTGAAGTGAGTGGTGCAATCGTTATCCCGCAAAAAGCCACGCAAGTTGAGCAA
GGTGGCGTCTATGTGATGATTGTGCTGCCCAATAATAAAGTTGAACGCCGCTTTATCGTGATAAAACATCAAGGTGAAAT
GGGCGTAGTAGTACAAAGTGGTTTAAAAGCGGGAGAGTTGGTCATTGTCGAAGGCATGCACCGTGTCCGTCATGGGCAAC
TGGCAGAGCCGTTAACTGCCGAGCAGTATAATAAGAAAGAGGATGTAAAACAAAAAGCGCAAGCATTGAAGCAACAAAAG
CTTGAGCAGGAACAGGGGGATAACTAA

Upstream 100 bases:

>100_bases
ATCTTTTTTACAAAAATAATTAGTGCTAATTCAATTACATCTAATTGATTTTACCAGCACCCTTTAGAGATTTGTGAACA
GCACATTTAGGGACGAACGT

Downstream 100 bases:

>100_bases
TGGCACAGTATTTTGTTAATCGCCCCGTATTTGCCAGTGTCATCTCTATTGTCATTGTGCTGCTGGGCTTAATTGCGATG
TTTCAATTACCCATTGACCA

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 408; Mature: 407

Protein sequence:

>408_residues
MPSHLNSTAFKLTLLFSSVALITGCEKEPVQITPKLVVVEKVSTATVPLYGNYVGVTKASLDVEVRARVDGFVENKTFIE
GSAVKEGALLYRIDNRPYVAVVNRLSANVESQQSALEKAQRDVERLKPLYEQDAASQLDFDNALSVLSQAKSSVAASKAE
LEEARLELSYTEIQSPISGLVSRSEVDIGALVGSSGQSLLTRVKQVDPIYVTFNMSALDYLNARRRMTSDKEQQAAESEG
KAVEGFVTITLPDNSEYRYLGDVGFTDPSVNPETGTFQVRAELPNPDKELLPGQYTNVRIKLSEVSGAIVIPQKATQVEQ
GGVYVMIVLPNNKVERRFIVIKHQGEMGVVVQSGLKAGELVIVEGMHRVRHGQLAEPLTAEQYNKKEDVKQKAQALKQQK
LEQEQGDN

Sequences:

>Translated_408_residues
MPSHLNSTAFKLTLLFSSVALITGCEKEPVQITPKLVVVEKVSTATVPLYGNYVGVTKASLDVEVRARVDGFVENKTFIE
GSAVKEGALLYRIDNRPYVAVVNRLSANVESQQSALEKAQRDVERLKPLYEQDAASQLDFDNALSVLSQAKSSVAASKAE
LEEARLELSYTEIQSPISGLVSRSEVDIGALVGSSGQSLLTRVKQVDPIYVTFNMSALDYLNARRRMTSDKEQQAAESEG
KAVEGFVTITLPDNSEYRYLGDVGFTDPSVNPETGTFQVRAELPNPDKELLPGQYTNVRIKLSEVSGAIVIPQKATQVEQ
GGVYVMIVLPNNKVERRFIVIKHQGEMGVVVQSGLKAGELVIVEGMHRVRHGQLAEPLTAEQYNKKEDVKQKAQALKQQK
LEQEQGDN
>Mature_407_residues
PSHLNSTAFKLTLLFSSVALITGCEKEPVQITPKLVVVEKVSTATVPLYGNYVGVTKASLDVEVRARVDGFVENKTFIEG
SAVKEGALLYRIDNRPYVAVVNRLSANVESQQSALEKAQRDVERLKPLYEQDAASQLDFDNALSVLSQAKSSVAASKAEL
EEARLELSYTEIQSPISGLVSRSEVDIGALVGSSGQSLLTRVKQVDPIYVTFNMSALDYLNARRRMTSDKEQQAAESEGK
AVEGFVTITLPDNSEYRYLGDVGFTDPSVNPETGTFQVRAELPNPDKELLPGQYTNVRIKLSEVSGAIVIPQKATQVEQG
GVYVMIVLPNNKVERRFIVIKHQGEMGVVVQSGLKAGELVIVEGMHRVRHGQLAEPLTAEQYNKKEDVKQKAQALKQQKL
EQEQGDN

Specific function: May contribute to resistance to some drugs, such as deoxycholate, sodium dodecyl sulfate and nalidixic acid, in the absence of BepD and BepE [H]

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789929, Length=388, Percent_Identity=31.9587628865979, Blast_Score=177, Evalue=1e-45,
Organism=Escherichia coli, GI1786668, Length=369, Percent_Identity=33.8753387533875, Blast_Score=169, Evalue=3e-43,
Organism=Escherichia coli, GI1789665, Length=365, Percent_Identity=33.4246575342466, Blast_Score=164, Evalue=8e-42,
Organism=Escherichia coli, GI87082036, Length=344, Percent_Identity=27.3255813953488, Blast_Score=136, Evalue=3e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 44781; Mature: 44650

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSHLNSTAFKLTLLFSSVALITGCEKEPVQITPKLVVVEKVSTATVPLYGNYVGVTKAS
CCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEECCEEEEEEECCCEEEEEECCEEEEEEEE
LDVEVRARVDGFVENKTFIEGSAVKEGALLYRIDNRPYVAVVNRLSANVESQQSALEKAQ
EEEEEEEEECCEECCCEEECCCCCCCCEEEEEECCCCEEEEEHHHHCCHHHHHHHHHHHH
RDVERLKPLYEQDAASQLDFDNALSVLSQAKSSVAASKAELEEARLELSYTEIQSPISGL
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHH
VSRSEVDIGALVGSSGQSLLTRVKQVDPIYVTFNMSALDYLNARRRMTSDKEQQAAESEG
HHCCCCCEEEEECCCCHHHHHHHHHCCCEEEEEECHHHHHHHHHHHCCCCHHHHHHHHCC
KAVEGFVTITLPDNSEYRYLGDVGFTDPSVNPETGTFQVRAELPNPDKELLPGQYTNVRI
CEEEEEEEEEECCCCCEEEECCCCCCCCCCCCCCCEEEEEEECCCCCHHHCCCCCCEEEE
KLSEVSGAIVIPQKATQVEQGGVYVMIVLPNNKVERRFIVIKHQGEMGVVVQSGLKAGEL
EEEECCCEEEECCCCCHHCCCCEEEEEEECCCCCCEEEEEEEECCCEEEEEECCCCCCCE
VIVEGMHRVRHGQLAEPLTAEQYNKKEDVKQKAQALKQQKLEQEQGDN
EEEECHHHHHCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PSHLNSTAFKLTLLFSSVALITGCEKEPVQITPKLVVVEKVSTATVPLYGNYVGVTKAS
CCCCCCHHHHHHHHHHHHHHHCCCCCCCCEECCEEEEEEECCCEEEEEECCEEEEEEEE
LDVEVRARVDGFVENKTFIEGSAVKEGALLYRIDNRPYVAVVNRLSANVESQQSALEKAQ
EEEEEEEEECCEECCCEEECCCCCCCCEEEEEECCCCEEEEEHHHHCCHHHHHHHHHHHH
RDVERLKPLYEQDAASQLDFDNALSVLSQAKSSVAASKAELEEARLELSYTEIQSPISGL
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHH
VSRSEVDIGALVGSSGQSLLTRVKQVDPIYVTFNMSALDYLNARRRMTSDKEQQAAESEG
HHCCCCCEEEEECCCCHHHHHHHHHCCCEEEEEECHHHHHHHHHHHCCCCHHHHHHHHCC
KAVEGFVTITLPDNSEYRYLGDVGFTDPSVNPETGTFQVRAELPNPDKELLPGQYTNVRI
CEEEEEEEEEECCCCCEEEECCCCCCCCCCCCCCCEEEEEEECCCCCHHHCCCCCCEEEE
KLSEVSGAIVIPQKATQVEQGGVYVMIVLPNNKVERRFIVIKHQGEMGVVVQSGLKAGEL
EEEECCCEEEECCCCCHHCCCCEEEEEEECCCCCCEEEEEEEECCCEEEEEECCCCCCCE
VIVEGMHRVRHGQLAEPLTAEQYNKKEDVKQKAQALKQQKLEQEQGDN
EEEECHHHHHCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12271122 [H]