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The map label for this gene is yhaJ [H]

Identifier: 157961699

GI number: 157961699

Start: 2286427

End: 2287326

Strand: Direct

Name: yhaJ [H]

Synonym: Spea_1875

Alternate gene names: 157961699

Gene position: 2286427-2287326 (Clockwise)

Preceding gene: 157961696

Following gene: 157961700

Centisome position: 44.19

GC content: 46.11

Gene sequence:

>900_bases
ATGCGTATTGATGTTGATGCTTTTAAAGTGCTGCAAGTGTTAGTTGAAGAAGGCAGCTTTGCTAAGGCAGCCGAGCGCCT
TCACAAGGCACAATCAGCGGTGAGCTACCAAGTTAAAAAGCTTGAGCAGCATCTAGGTGTAAACCTTTTTTGTCGGGATC
AGTATCGAGCAGAGTTAACCCCTGAGGGAAAAGTTATCTTGGCCGAGGGACAAAGGTTACTGCAGTATCTGGCAAATATT
GAACATCTAGCCAGCCGATTTAGCGAAGGCTGGGAGCCTAAGCTTGAAATTATCATCGACGGGGCATTGCCGATGAAACC
TATTATGAGAGCATTGAAGCGACTTTCTGAGCAACAGATCCCTACTAAAGTGCAGCTTAATATGGAGTTTCTTGGAGGCG
TTCAAGATAGGTTCGAGCGAGACAGTGCCGATCTTATGTTGGTAAAAGACTATCGTACTGGCCCAAGTTACCGTCCTCAG
GCTTTACCGCCGATCACCAGTGTTTTAGTCGTCAGCGCAGAGCATCCGCTTGCTCAAGAAAAGCGGCTCAGTTTATTTGA
GTTACAGCAACATGTGGAGCTAACCATTGAAGACTCATCGCCTGATAAACACTACCGTGACGAGTTACAGTTTGGTGGCG
ATAAGGTGTTCTACCTTTCAGGTTTTATCATGAAGAAGAATGCACTTTTGATGGGGCTAGGTTTTGGCTGGATGCCAGAC
TTTCTAATCGAAAAAGAGCTTAGTAGTGGCGAATTAGTCGAAGTGGACTTTAGCGGAGGTAATCGCTATAGCTTCACTCC
TCAACTGGTTTCAACCATGGAGCGGCCTCTTGGGCGAGCGGGACGTTTGTTCACTGAATTGGTAATGGAAGAGTGTAGGA
GTGAGACTAACTTAGTTTAA

Upstream 100 bases:

>100_bases
TTCAATTTAGTTGCTTGAATGAACAAAAAAAAGAATAATAATTAGCCTAACTTAATCATATTTTTAGATGGTAATTTATT
GTTTACGTCGGAGGGGCTAA

Downstream 100 bases:

>100_bases
CTTAACCTTAGTTTAGTTTAGTTTAGTTTAGTTTAGTTTAGTTTAGTTTAGTTTAGTTTAGTTTAGTTTAGTTTAGTTTA
GCGAGCCATTGCAGATTGCA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MRIDVDAFKVLQVLVEEGSFAKAAERLHKAQSAVSYQVKKLEQHLGVNLFCRDQYRAELTPEGKVILAEGQRLLQYLANI
EHLASRFSEGWEPKLEIIIDGALPMKPIMRALKRLSEQQIPTKVQLNMEFLGGVQDRFERDSADLMLVKDYRTGPSYRPQ
ALPPITSVLVVSAEHPLAQEKRLSLFELQQHVELTIEDSSPDKHYRDELQFGGDKVFYLSGFIMKKNALLMGLGFGWMPD
FLIEKELSSGELVEVDFSGGNRYSFTPQLVSTMERPLGRAGRLFTELVMEECRSETNLV

Sequences:

>Translated_299_residues
MRIDVDAFKVLQVLVEEGSFAKAAERLHKAQSAVSYQVKKLEQHLGVNLFCRDQYRAELTPEGKVILAEGQRLLQYLANI
EHLASRFSEGWEPKLEIIIDGALPMKPIMRALKRLSEQQIPTKVQLNMEFLGGVQDRFERDSADLMLVKDYRTGPSYRPQ
ALPPITSVLVVSAEHPLAQEKRLSLFELQQHVELTIEDSSPDKHYRDELQFGGDKVFYLSGFIMKKNALLMGLGFGWMPD
FLIEKELSSGELVEVDFSGGNRYSFTPQLVSTMERPLGRAGRLFTELVMEECRSETNLV
>Mature_299_residues
MRIDVDAFKVLQVLVEEGSFAKAAERLHKAQSAVSYQVKKLEQHLGVNLFCRDQYRAELTPEGKVILAEGQRLLQYLANI
EHLASRFSEGWEPKLEIIIDGALPMKPIMRALKRLSEQQIPTKVQLNMEFLGGVQDRFERDSADLMLVKDYRTGPSYRPQ
ALPPITSVLVVSAEHPLAQEKRLSLFELQQHVELTIEDSSPDKHYRDELQFGGDKVFYLSGFIMKKNALLMGLGFGWMPD
FLIEKELSSGELVEVDFSGGNRYSFTPQLVSTMERPLGRAGRLFTELVMEECRSETNLV

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789492, Length=253, Percent_Identity=27.6679841897233, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI87082024, Length=257, Percent_Identity=27.6264591439689, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1786713, Length=257, Percent_Identity=28.0155642023346, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1786508, Length=240, Percent_Identity=25, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1787949, Length=254, Percent_Identity=24.0157480314961, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1790262, Length=188, Percent_Identity=31.9148936170213, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1786401, Length=270, Percent_Identity=27.4074074074074, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI87082132, Length=299, Percent_Identity=25.4180602006689, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34008; Mature: 34008

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIDVDAFKVLQVLVEEGSFAKAAERLHKAQSAVSYQVKKLEQHLGVNLFCRDQYRAELT
CCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCC
PEGKVILAEGQRLLQYLANIEHLASRFSEGWEPKLEIIIDGALPMKPIMRALKRLSEQQI
CCCCEEEECHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHHCC
PTKVQLNMEFLGGVQDRFERDSADLMLVKDYRTGPSYRPQALPPITSVLVVSAEHPLAQE
CCEEEECHHHHCCHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCHHHEEEEECCCCCCHH
KRLSLFELQQHVELTIEDSSPDKHYRDELQFGGDKVFYLSGFIMKKNALLMGLGFGWMPD
HHHHHHHHHHHEEEEEECCCCCHHHHHHHHCCCCEEEEEECEEEECCEEEEECCCCCCCH
FLIEKELSSGELVEVDFSGGNRYSFTPQLVSTMERPLGRAGRLFTELVMEECRSETNLV
HHHHHCCCCCCEEEEEECCCCEEEECHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRIDVDAFKVLQVLVEEGSFAKAAERLHKAQSAVSYQVKKLEQHLGVNLFCRDQYRAELT
CCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCC
PEGKVILAEGQRLLQYLANIEHLASRFSEGWEPKLEIIIDGALPMKPIMRALKRLSEQQI
CCCCEEEECHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHHCC
PTKVQLNMEFLGGVQDRFERDSADLMLVKDYRTGPSYRPQALPPITSVLVVSAEHPLAQE
CCEEEECHHHHCCHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCHHHEEEEECCCCCCHH
KRLSLFELQQHVELTIEDSSPDKHYRDELQFGGDKVFYLSGFIMKKNALLMGLGFGWMPD
HHHHHHHHHHHEEEEEECCCCCHHHHHHHHCCCCEEEEEECEEEECCEEEEECCCCCCCH
FLIEKELSSGELVEVDFSGGNRYSFTPQLVSTMERPLGRAGRLFTELVMEECRSETNLV
HHHHHCCCCCCEEEEEECCCCEEEECHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]