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The map label for this gene is bioF [H]

Identifier: 157961626

GI number: 157961626

Start: 2201013

End: 2202206

Strand: Direct

Name: bioF [H]

Synonym: Spea_1802

Alternate gene names: 157961626

Gene position: 2201013-2202206 (Clockwise)

Preceding gene: 157961625

Following gene: 157961627

Centisome position: 42.54

GC content: 46.73

Gene sequence:

>1194_bases
ATGTCAGAAACTTCCGTTAATCACCCAGCCAAAGAGTCATTGACCGCGCGAGTGGTTACAAAAAATCGCTCATTGGATGA
GCAAGGGCTGCTAAGGCAGCGAATAAACCTTGTTTTAGATCCTAGTCAGTCAAACTACTTCGAAGTCGACGCGCTCCCTT
ACGTCAACTTCAGTAGTAATGACTATTTGGGACTAAGCACTTCGGCCACCATCGCTGATGCTCTTTATCAGGGAGCAAAA
GATTATGGTGTAGGTAGCGCCGCATCGCCTTTAGTGGTTGGATACAGCCAAGCACATCTGCAGCTTGAACAGGCCTTATG
TGAAGCTACGGGACATGAGGCCGCAATATTATTTTGCTCAGGATTTAGCGCTAATACGGCACTGATGAAGACGCTGTTTA
ATGAAACCGACACAGTGCTAGCCGATAAGCTTATCCATGCTTCGGTTATTGATGGCGTACAAGATAGCGGCGCTAAGCTT
AAGCGGTTTGCGCATAATGATATCGAAAGCGCTAAAACAGATTTAGAACGCTTTACCCCTAGTGCCTTAATTACCGAGAG
CGTGTTTAGTATGGACGGTGATATAGCACCGTTAGCCGAACTTTCAGCGCTTTGTCAGCAGCATAACAGCTGGCTAATCG
TAGATGATGCTCATGGCTTCGGAGTCATAGGTGAAAATGGTCTCGGGGCTTCTAATTTATCCGCTGAGATCAAAATTGAT
GTGCAATTGGTGACTTTCGGTAAAGCCATGGGCGGCCAAGGTGCAGCCATTCTAGGCAGTCAAGATTTGATAGATTTCTT
AGTGGCTAATGCCCGTCAGTATATCTATTCGACGGCTTTATCACCAGCAAGCGCAGCAGCGGCTTATGCGGCGATAAAAA
TGATAAGAGAACAGCCTGAACTCCGAGAAAAGCTCACTGAGAACATTTTGTGTTTTAGGGAGGCTTGTGCTGCTAATACT
ATCGCTCTGACTGAGTCACAAACCGCCATTCAGCCCATAATTCTTGGGGGCGCGGCAAAGACGATGGCGGTTGCAAAAAG
ATTGAAAGAACATGGTTTTTGGCTAGGAGCCATCCGACCTCCAACTGTTGCGAAGGATAGCGCAAGACTAAGGTTGACGT
TGAATGCTAACCATTGCCAGCGAGATATTTTGGCGCTAGTGGATGTGTTGGCGACCATATTGAACGATGATTAA

Upstream 100 bases:

>100_bases
TCACATGTACGCCCCATGTGATGATGAACGAGAACAAGATGACCCGCTTTAAATTGAGGCGGGTTTATTAGGTAAATTAT
TCTAAAATCGAGCAAGCTTA

Downstream 100 bases:

>100_bases
TGTGAGTGGGCATTCAGCCTTAACTCATTTTTAAAATTTAAACTAGAAACTATCAAAGTAATTGATTGTTTAGGATCCAA
TGCAGTGAATACAGGATCAG

Product: class I/II aminotransferase

Products: NA

Alternate protein names: AONS; 7-keto-8-amino-pelargonic acid synthase; 7-KAP synthase; KAPA synthase; 8-amino-7-ketopelargonate synthase; L-alanine--pimeloyl-CoA ligase [H]

Number of amino acids: Translated: 397; Mature: 396

Protein sequence:

>397_residues
MSETSVNHPAKESLTARVVTKNRSLDEQGLLRQRINLVLDPSQSNYFEVDALPYVNFSSNDYLGLSTSATIADALYQGAK
DYGVGSAASPLVVGYSQAHLQLEQALCEATGHEAAILFCSGFSANTALMKTLFNETDTVLADKLIHASVIDGVQDSGAKL
KRFAHNDIESAKTDLERFTPSALITESVFSMDGDIAPLAELSALCQQHNSWLIVDDAHGFGVIGENGLGASNLSAEIKID
VQLVTFGKAMGGQGAAILGSQDLIDFLVANARQYIYSTALSPASAAAAYAAIKMIREQPELREKLTENILCFREACAANT
IALTESQTAIQPIILGGAAKTMAVAKRLKEHGFWLGAIRPPTVAKDSARLRLTLNANHCQRDILALVDVLATILNDD

Sequences:

>Translated_397_residues
MSETSVNHPAKESLTARVVTKNRSLDEQGLLRQRINLVLDPSQSNYFEVDALPYVNFSSNDYLGLSTSATIADALYQGAK
DYGVGSAASPLVVGYSQAHLQLEQALCEATGHEAAILFCSGFSANTALMKTLFNETDTVLADKLIHASVIDGVQDSGAKL
KRFAHNDIESAKTDLERFTPSALITESVFSMDGDIAPLAELSALCQQHNSWLIVDDAHGFGVIGENGLGASNLSAEIKID
VQLVTFGKAMGGQGAAILGSQDLIDFLVANARQYIYSTALSPASAAAAYAAIKMIREQPELREKLTENILCFREACAANT
IALTESQTAIQPIILGGAAKTMAVAKRLKEHGFWLGAIRPPTVAKDSARLRLTLNANHCQRDILALVDVLATILNDD
>Mature_396_residues
SETSVNHPAKESLTARVVTKNRSLDEQGLLRQRINLVLDPSQSNYFEVDALPYVNFSSNDYLGLSTSATIADALYQGAKD
YGVGSAASPLVVGYSQAHLQLEQALCEATGHEAAILFCSGFSANTALMKTLFNETDTVLADKLIHASVIDGVQDSGAKLK
RFAHNDIESAKTDLERFTPSALITESVFSMDGDIAPLAELSALCQQHNSWLIVDDAHGFGVIGENGLGASNLSAEIKIDV
QLVTFGKAMGGQGAAILGSQDLIDFLVANARQYIYSTALSPASAAAAYAAIKMIREQPELREKLTENILCFREACAANTI
ALTESQTAIQPIILGGAAKTMAVAKRLKEHGFWLGAIRPPTVAKDSARLRLTLNANHCQRDILALVDVLATILNDD

Specific function: Catalyzes the decarboxylative condensation of pimeloyl- CoA and L-alanine to produce 8-amino-7-oxononanoate (AON), coenzyme A, and carbon dioxide [H]

COG id: COG0156

COG function: function code H; 7-keto-8-aminopelargonate synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. BioF subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657118, Length=363, Percent_Identity=28.9256198347107, Blast_Score=168, Evalue=1e-41,
Organism=Homo sapiens, GI284448556, Length=344, Percent_Identity=29.3604651162791, Blast_Score=166, Evalue=5e-41,
Organism=Homo sapiens, GI83977442, Length=343, Percent_Identity=31.1953352769679, Blast_Score=157, Evalue=1e-38,
Organism=Homo sapiens, GI83977440, Length=343, Percent_Identity=31.1953352769679, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI83977444, Length=343, Percent_Identity=31.1953352769679, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI4502025, Length=331, Percent_Identity=30.2114803625378, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI40316939, Length=331, Percent_Identity=30.2114803625378, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI4758668, Length=353, Percent_Identity=27.1954674220963, Blast_Score=135, Evalue=8e-32,
Organism=Homo sapiens, GI119220554, Length=363, Percent_Identity=26.9972451790634, Blast_Score=133, Evalue=4e-31,
Organism=Homo sapiens, GI5454084, Length=288, Percent_Identity=27.4305555555556, Blast_Score=95, Evalue=9e-20,
Organism=Escherichia coli, GI1786993, Length=354, Percent_Identity=45.7627118644068, Blast_Score=273, Evalue=1e-74,
Organism=Escherichia coli, GI1790046, Length=373, Percent_Identity=32.171581769437, Blast_Score=204, Evalue=1e-53,
Organism=Caenorhabditis elegans, GI71994529, Length=347, Percent_Identity=31.9884726224784, Blast_Score=197, Evalue=1e-50,
Organism=Caenorhabditis elegans, GI32566772, Length=410, Percent_Identity=25.1219512195122, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17560914, Length=368, Percent_Identity=26.0869565217391, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17560912, Length=368, Percent_Identity=26.0869565217391, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI71982617, Length=340, Percent_Identity=25, Blast_Score=105, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI71982625, Length=275, Percent_Identity=25.4545454545455, Blast_Score=105, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6320438, Length=369, Percent_Identity=27.3712737127371, Blast_Score=145, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6320267, Length=353, Percent_Identity=24.0793201133144, Blast_Score=108, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6323954, Length=255, Percent_Identity=25.4901960784314, Blast_Score=102, Evalue=9e-23,
Organism=Drosophila melanogaster, GI24662918, Length=337, Percent_Identity=31.7507418397626, Blast_Score=182, Evalue=4e-46,
Organism=Drosophila melanogaster, GI17137420, Length=398, Percent_Identity=28.894472361809, Blast_Score=157, Evalue=2e-38,
Organism=Drosophila melanogaster, GI17136286, Length=370, Percent_Identity=28.1081081081081, Blast_Score=127, Evalue=9e-30,
Organism=Drosophila melanogaster, GI24653280, Length=277, Percent_Identity=26.7148014440433, Blast_Score=108, Evalue=7e-24,
Organism=Drosophila melanogaster, GI24653276, Length=277, Percent_Identity=26.7148014440433, Blast_Score=108, Evalue=7e-24,
Organism=Drosophila melanogaster, GI24653278, Length=277, Percent_Identity=26.7148014440433, Blast_Score=108, Evalue=7e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022834
- InterPro:   IPR001917
- InterPro:   IPR004839
- InterPro:   IPR004723
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00155 Aminotran_1_2 [H]

EC number: =2.3.1.47 [H]

Molecular weight: Translated: 42469; Mature: 42337

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS00599 AA_TRANSFER_CLASS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSETSVNHPAKESLTARVVTKNRSLDEQGLLRQRINLVLDPSQSNYFEVDALPYVNFSSN
CCCCCCCCCHHHHHEEEEEECCCCCCHHHHHHHHCCEEECCCCCCEEEEECCEEEECCCC
DYLGLSTSATIADALYQGAKDYGVGSAASPLVVGYSQAHLQLEQALCEATGHEAAILFCS
CEEECCCHHHHHHHHHHCCHHCCCCCCCCCEEEECHHHHHHHHHHHHHCCCCCEEEEEEE
GFSANTALMKTLFNETDTVLADKLIHASVIDGVQDSGAKLKRFAHNDIESAKTDLERFTP
CCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
SALITESVFSMDGDIAPLAELSALCQQHNSWLIVDDAHGFGVIGENGLGASNLSAEIKID
HHHHHHHHHHCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCEECCCCCCCCCCCEEEEEE
VQLVTFGKAMGGQGAAILGSQDLIDFLVANARQYIYSTALSPASAAAAYAAIKMIREQPE
EEEEEECCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHH
LREKLTENILCFREACAANTIALTESQTAIQPIILGGAAKTMAVAKRLKEHGFWLGAIRP
HHHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEEECCCHHHHHHHHHHHHCCEEEECCCC
PTVAKDSARLRLTLNANHCQRDILALVDVLATILNDD
CCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SETSVNHPAKESLTARVVTKNRSLDEQGLLRQRINLVLDPSQSNYFEVDALPYVNFSSN
CCCCCCCCHHHHHEEEEEECCCCCCHHHHHHHHCCEEECCCCCCEEEEECCEEEECCCC
DYLGLSTSATIADALYQGAKDYGVGSAASPLVVGYSQAHLQLEQALCEATGHEAAILFCS
CEEECCCHHHHHHHHHHCCHHCCCCCCCCCEEEECHHHHHHHHHHHHHCCCCCEEEEEEE
GFSANTALMKTLFNETDTVLADKLIHASVIDGVQDSGAKLKRFAHNDIESAKTDLERFTP
CCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCC
SALITESVFSMDGDIAPLAELSALCQQHNSWLIVDDAHGFGVIGENGLGASNLSAEIKID
HHHHHHHHHHCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCEECCCCCCCCCCCEEEEEE
VQLVTFGKAMGGQGAAILGSQDLIDFLVANARQYIYSTALSPASAAAAYAAIKMIREQPE
EEEEEECCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHH
LREKLTENILCFREACAANTIALTESQTAIQPIILGGAAKTMAVAKRLKEHGFWLGAIRP
HHHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEEECCCHHHHHHHHHHHHCCEEEECCCC
PTVAKDSARLRLTLNANHCQRDILALVDVLATILNDD
CCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA