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The map label for this gene is bioB [H]

Identifier: 157961625

GI number: 157961625

Start: 2199684

End: 2200736

Strand: Direct

Name: bioB [H]

Synonym: Spea_1801

Alternate gene names: 157961625

Gene position: 2199684-2200736 (Clockwise)

Preceding gene: 157961622

Following gene: 157961626

Centisome position: 42.51

GC content: 45.58

Gene sequence:

>1053_bases
ATGCCTGAAGTACAGCTAAGAAATAACTGGAAACGTGAAGAGATAGAAGCCTTATTTGCACTGCCGATGAACGACCTTCT
TTTTCAGGCGCATAGCATTCATCGCCAAGAGTTTGATCCTAACGAAGTTCAGGTCAGTCGTTTACTGTCGATTAAAACCG
GAGCTTGTCCTGAAGACTGTAAATATTGTCCACAGAGTGCACGCTATGATACAGGTCTTGAGAAAGAGCGCTTACTCGCG
ATGGAAACGGTATTAACCGAAGCGCGCAGTGCAAAAGCTGCGGGGGCATCACGTTTCTGCATGGGAGCCGCTTGGCGCAA
TCCAAAAGAACGTGACATGCCATATCTGAAAACCATGGTTGAAGAAGTAAAAGCGCTTGGTATGGAAACCTGTATGACCT
TAGGCATGTTGTCAGCTGAACAAGCCAATACGCTTGCAGACGCAGGTTTAGATTATTACAACCATAACTTAGATACCTCG
CCTGAATATTATGGCGATGTCATCACTACCCGTACCTATCAGAGCCGCCTTGATACCTTAACTAACGTACGAGCTTCAGG
CATGAAAGTTTGCTCTGGTGGTATCGTAGGAATGGGCGAGAAGGCCACCGACAGAGCGGGACTTATCCAGCAACTCGCTA
ACTTAGAGCAACATCCCGATTCTGTACCAATTAATATGTTGGTGAAAGTTGAAGGTACGCCTTTTGAGAAACTTGATGAT
CTCGACCCATTAGAGTTTGTGCGCACGATTGCAGTAGCTCGAATTACTATGCCTAAATCACGAGTTCGTTTATCTGCCGG
TCGTGAAAACATGAGCGATGAGTTACAGGCTATGTGTTTCTTTGCTGGCGCTAACTCAATCTTTTACGGATGTAAGTTAC
TGACGACACCAAATCCAGAAGAGAATGATGATATGAGTCTGTTCAAACGTCTCGGTTTACATCCTGAGCAGGGTATTGCT
GCGACAAAAGAGCAAGATGAAGCCATGCTAGCTAAAGCAGCGGCACATCAGGATAAAAAGTCAGCTGCATTTTATGATGC
TGGTGCGCTCTAA

Upstream 100 bases:

>100_bases
GGAGTACAAACTAATTAAGCATGTGGATTGTTAATTAGGTTTGTGACTGCGTGATAATATACAGTATTTAGATGAATGTG
TGAGTTAACAAGGGAACAAA

Downstream 100 bases:

>100_bases
GAATCGATATCAATTGCTTTCGTAACAGTTAGGGCATTGAATAGTTCTATTATTAAAAAATAACGGAATTACACCATTAG
CCATTATGAAAACTATTCAC

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 350; Mature: 349

Protein sequence:

>350_residues
MPEVQLRNNWKREEIEALFALPMNDLLFQAHSIHRQEFDPNEVQVSRLLSIKTGACPEDCKYCPQSARYDTGLEKERLLA
METVLTEARSAKAAGASRFCMGAAWRNPKERDMPYLKTMVEEVKALGMETCMTLGMLSAEQANTLADAGLDYYNHNLDTS
PEYYGDVITTRTYQSRLDTLTNVRASGMKVCSGGIVGMGEKATDRAGLIQQLANLEQHPDSVPINMLVKVEGTPFEKLDD
LDPLEFVRTIAVARITMPKSRVRLSAGRENMSDELQAMCFFAGANSIFYGCKLLTTPNPEENDDMSLFKRLGLHPEQGIA
ATKEQDEAMLAKAAAHQDKKSAAFYDAGAL

Sequences:

>Translated_350_residues
MPEVQLRNNWKREEIEALFALPMNDLLFQAHSIHRQEFDPNEVQVSRLLSIKTGACPEDCKYCPQSARYDTGLEKERLLA
METVLTEARSAKAAGASRFCMGAAWRNPKERDMPYLKTMVEEVKALGMETCMTLGMLSAEQANTLADAGLDYYNHNLDTS
PEYYGDVITTRTYQSRLDTLTNVRASGMKVCSGGIVGMGEKATDRAGLIQQLANLEQHPDSVPINMLVKVEGTPFEKLDD
LDPLEFVRTIAVARITMPKSRVRLSAGRENMSDELQAMCFFAGANSIFYGCKLLTTPNPEENDDMSLFKRLGLHPEQGIA
ATKEQDEAMLAKAAAHQDKKSAAFYDAGAL
>Mature_349_residues
PEVQLRNNWKREEIEALFALPMNDLLFQAHSIHRQEFDPNEVQVSRLLSIKTGACPEDCKYCPQSARYDTGLEKERLLAM
ETVLTEARSAKAAGASRFCMGAAWRNPKERDMPYLKTMVEEVKALGMETCMTLGMLSAEQANTLADAGLDYYNHNLDTSP
EYYGDVITTRTYQSRLDTLTNVRASGMKVCSGGIVGMGEKATDRAGLIQQLANLEQHPDSVPINMLVKVEGTPFEKLDDL
DPLEFVRTIAVARITMPKSRVRLSAGRENMSDELQAMCFFAGANSIFYGCKLLTTPNPEENDDMSLFKRLGLHPEQGIAA
TKEQDEAMLAKAAAHQDKKSAAFYDAGAL

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism [H]

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786992, Length=345, Percent_Identity=65.2173913043478, Blast_Score=477, Evalue=1e-136,
Organism=Saccharomyces cerevisiae, GI6321725, Length=316, Percent_Identity=47.4683544303797, Blast_Score=311, Evalue=9e-86,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: =2.8.1.6 [H]

Molecular weight: Translated: 38865; Mature: 38734

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
7.1 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
6.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEVQLRNNWKREEIEALFALPMNDLLFQAHSIHRQEFDPNEVQVSRLLSIKTGACPEDC
CCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCHHH
KYCPQSARYDTGLEKERLLAMETVLTEARSAKAAGASRFCMGAAWRNPKERDMPYLKTMV
HHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCHHHHHHH
EEVKALGMETCMTLGMLSAEQANTLADAGLDYYNHNLDTSPEYYGDVITTRTYQSRLDTL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCCHHHCCHHHHHHHHHHHHHH
TNVRASGMKVCSGGIVGMGEKATDRAGLIQQLANLEQHPDSVPINMLVKVEGTPFEKLDD
HHHHHCCCEEECCCCEECCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHCC
LDPLEFVRTIAVARITMPKSRVRLSAGRENMSDELQAMCFFAGANSIFYGCKLLTTPNPE
CCHHHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHHHHHHCCCCCEECEEEEECCCCC
ENDDMSLFKRLGLHPEQGIAATKEQDEAMLAKAAAHQDKKSAAFYDAGAL
CCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHCCCCCCC
>Mature Secondary Structure 
PEVQLRNNWKREEIEALFALPMNDLLFQAHSIHRQEFDPNEVQVSRLLSIKTGACPEDC
CCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCHHH
KYCPQSARYDTGLEKERLLAMETVLTEARSAKAAGASRFCMGAAWRNPKERDMPYLKTMV
HHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCHHHHHHH
EEVKALGMETCMTLGMLSAEQANTLADAGLDYYNHNLDTSPEYYGDVITTRTYQSRLDTL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCCHHHCCHHHHHHHHHHHHHH
TNVRASGMKVCSGGIVGMGEKATDRAGLIQQLANLEQHPDSVPINMLVKVEGTPFEKLDD
HHHHHCCCEEECCCCEECCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHCC
LDPLEFVRTIAVARITMPKSRVRLSAGRENMSDELQAMCFFAGANSIFYGCKLLTTPNPE
CCHHHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHHHHHHCCCCCEECEEEEECCCCC
ENDDMSLFKRLGLHPEQGIAATKEQDEAMLAKAAAHQDKKSAAFYDAGAL
CCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA