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The map label for this gene is phbC [H]

Identifier: 157961576

GI number: 157961576

Start: 2129463

End: 2131217

Strand: Direct

Name: phbC [H]

Synonym: Spea_1752

Alternate gene names: 157961576

Gene position: 2129463-2131217 (Clockwise)

Preceding gene: 157961575

Following gene: 157961577

Centisome position: 41.15

GC content: 44.1

Gene sequence:

>1755_bases
ATGGAAAGTAAATCGCCCTTTCAAGACGCGATCGACAATGCAATGCAATTTGGCCAAGCATGGATGGATAGCTTCGGGCA
GTCGGCTCAATCCTCTATCGTAGAAACACAAGCGGAAGATTGGGCACAATGGATGCGCTCATCGGTTGAACACCCTGTTA
ACTCTATTGAACAACAAATGGATTGGTGGGGGCAACAAGTCAATCTATTTAACGATTGCATTATGAGCAGTCCCGCAGAA
AAAGAGACAGATCGACGATTTAAAGACCCTGCGTGGAATGAACAGGCACTTTATAAATACATCAAAGAAAGTTATAAGTT
GGCTTGTAATAATATTCAAGCATCAATCAATAACACCGAAGGGCTAGATGACGAAACTCGTCAGCGATTATCCTTTTTTT
CTCGCCAGTATCTTAATGCTATGTCACCGAGCAATTTTGTTGCCACCAACCCTGAAATAATGAAGCTAACCATTGAGAGT
AAAGGCCAAAACCTAATTAAAGGGCTAGAGCAACTTCAGCAAGATTTAGAGCAAAGCGTCGATACCTTGAATATTCGCAT
GACAGATAAAACGGCTTTCACTGTAGGAAAAAATATTGCCACAACTCCTGGTAAGGTTGTCTTTAAAAACGATCTGTTTG
AACTTATTCAATATCAAGCCACAACGGAGCAAGTGTACAAACGACCTCTGCTTGTTGTACCGCCATTTGTGAACAAATTT
TATATCATGGATCTAAGCCCTGAGCGTTCTTACACGCAATGGCTAGTGAGCCAAGGGCATACAGTATTTATGATCTCTTG
GGTTAACCCCAATGCCGAGATGGCAGCAACTGATTTTGGTGACTACGTTACTCAGGGGGTTATCCTTGCGTTAGATGCAA
TAGAAGCTGAAACCGGTGAACGTGAAGTCAACGGTATTGGTTATTGTATCGGTGGGACACTGTTAACTGCAGCAATGGCT
TATTTAGCCGGCAAACGCCGCAAGCAAAGAGTCAAATCAGCAACCTTACTCACCACCATTCTAGACTTTGGCCAACCTGG
CGAACTTGGTGTGTTCATCAACGACCCACTGATTAGCAGTATCGAAGCGCAGAATAATGCTCGTGGCTACATGGATGGAC
GACAGATGGCTGTGTCATTTAGCCTGTTAAGGGAAAATAGCCTGTATTGGAATTACTATGTGACCAACTACCTTAAAGGC
GAAAGTCCAGTGGCGTTCGATCTACTTCATTGGAATTGTGACAATACCAACATAACTGCTGCGACACACAATCAGATATT
ACGCCAAATGTACCTTGAAAATAAGCTGAAGGAACCTGGTGGCATCACAGTTGATGGCGTTAAAGTCGACCTGAGTAAAG
TTAAGTCTCCGTGCTATTTCCTCTCCGCTATTGAAGATCATATTGCAGTCTGGGAAGGCACTTTTCGTGGTACAGAGTTA
CTCAATGGCGATAACACCTTTGTGCTGGCCGAAAGTGGCCATATTGCAGGACCCATGAACCCGCCAAGTTCTAATAAATA
TGGTTTTTGGACCAATAGCGATAATGCGCAGAGCCCGGCGAAATGGCTGGCTGAGGCGGATAATCATTCTGGTTCTTGGT
GGCCCCACTGGCAATCTTGGGTCGATGAACGAAATTTCTCTGACAAAATAGCAGCACGTTCATTAACTGGGAAGTTAGAT
GCTCCGGGGGAGTACGTAAAACAACGAATCGAAGATGTGATAGCACCAAAAGAGGAGGTACGCCATGACACTTAA

Upstream 100 bases:

>100_bases
CGTAAAGGGTAATAGTGTGGCGTGCTGCTTGGCTAAGAACTGCACGAGCAAAAGTGCGTCACCTTTTTATTAGTTTTAAT
TTGCCACTAAGGACGCCATC

Downstream 100 bases:

>100_bases
AGTGGGCCAGAGCTCGATGATTGAAAAATGCTTAGATCAACAATCTGTAGTACAGTTTGCATCGTTAGCTGAGGATTATA
ACCCTGTGCATTTGGATGCC

Product: poly(R)-hydroxyalkanoic acid synthase, class I

Products: NA

Alternate protein names: PHA synthase; PHB synthase; Poly(3-hydroxyalkanoate) polymerase; PHA polymerase; Poly(3-hydroxybutyrate) polymerase; PHB polymerase; Polyhydroxyalkanoic acid synthase [H]

Number of amino acids: Translated: 584; Mature: 584

Protein sequence:

>584_residues
MESKSPFQDAIDNAMQFGQAWMDSFGQSAQSSIVETQAEDWAQWMRSSVEHPVNSIEQQMDWWGQQVNLFNDCIMSSPAE
KETDRRFKDPAWNEQALYKYIKESYKLACNNIQASINNTEGLDDETRQRLSFFSRQYLNAMSPSNFVATNPEIMKLTIES
KGQNLIKGLEQLQQDLEQSVDTLNIRMTDKTAFTVGKNIATTPGKVVFKNDLFELIQYQATTEQVYKRPLLVVPPFVNKF
YIMDLSPERSYTQWLVSQGHTVFMISWVNPNAEMAATDFGDYVTQGVILALDAIEAETGEREVNGIGYCIGGTLLTAAMA
YLAGKRRKQRVKSATLLTTILDFGQPGELGVFINDPLISSIEAQNNARGYMDGRQMAVSFSLLRENSLYWNYYVTNYLKG
ESPVAFDLLHWNCDNTNITAATHNQILRQMYLENKLKEPGGITVDGVKVDLSKVKSPCYFLSAIEDHIAVWEGTFRGTEL
LNGDNTFVLAESGHIAGPMNPPSSNKYGFWTNSDNAQSPAKWLAEADNHSGSWWPHWQSWVDERNFSDKIAARSLTGKLD
APGEYVKQRIEDVIAPKEEVRHDT

Sequences:

>Translated_584_residues
MESKSPFQDAIDNAMQFGQAWMDSFGQSAQSSIVETQAEDWAQWMRSSVEHPVNSIEQQMDWWGQQVNLFNDCIMSSPAE
KETDRRFKDPAWNEQALYKYIKESYKLACNNIQASINNTEGLDDETRQRLSFFSRQYLNAMSPSNFVATNPEIMKLTIES
KGQNLIKGLEQLQQDLEQSVDTLNIRMTDKTAFTVGKNIATTPGKVVFKNDLFELIQYQATTEQVYKRPLLVVPPFVNKF
YIMDLSPERSYTQWLVSQGHTVFMISWVNPNAEMAATDFGDYVTQGVILALDAIEAETGEREVNGIGYCIGGTLLTAAMA
YLAGKRRKQRVKSATLLTTILDFGQPGELGVFINDPLISSIEAQNNARGYMDGRQMAVSFSLLRENSLYWNYYVTNYLKG
ESPVAFDLLHWNCDNTNITAATHNQILRQMYLENKLKEPGGITVDGVKVDLSKVKSPCYFLSAIEDHIAVWEGTFRGTEL
LNGDNTFVLAESGHIAGPMNPPSSNKYGFWTNSDNAQSPAKWLAEADNHSGSWWPHWQSWVDERNFSDKIAARSLTGKLD
APGEYVKQRIEDVIAPKEEVRHDT
>Mature_584_residues
MESKSPFQDAIDNAMQFGQAWMDSFGQSAQSSIVETQAEDWAQWMRSSVEHPVNSIEQQMDWWGQQVNLFNDCIMSSPAE
KETDRRFKDPAWNEQALYKYIKESYKLACNNIQASINNTEGLDDETRQRLSFFSRQYLNAMSPSNFVATNPEIMKLTIES
KGQNLIKGLEQLQQDLEQSVDTLNIRMTDKTAFTVGKNIATTPGKVVFKNDLFELIQYQATTEQVYKRPLLVVPPFVNKF
YIMDLSPERSYTQWLVSQGHTVFMISWVNPNAEMAATDFGDYVTQGVILALDAIEAETGEREVNGIGYCIGGTLLTAAMA
YLAGKRRKQRVKSATLLTTILDFGQPGELGVFINDPLISSIEAQNNARGYMDGRQMAVSFSLLRENSLYWNYYVTNYLKG
ESPVAFDLLHWNCDNTNITAATHNQILRQMYLENKLKEPGGITVDGVKVDLSKVKSPCYFLSAIEDHIAVWEGTFRGTEL
LNGDNTFVLAESGHIAGPMNPPSSNKYGFWTNSDNAQSPAKWLAEADNHSGSWWPHWQSWVDERNFSDKIAARSLTGKLD
APGEYVKQRIEDVIAPKEEVRHDT

Specific function: Polymerizes d(-)-3-hydroxybutyryl-CoA to create PHB which consists of thousands of hydroxybutyrate molecules linked end to end. PHB serves as an intracellular energy reserve material when cells grow under conditions of nutrient limitation [H]

COG id: COG3243

COG function: function code I; Poly(3-hydroxyalkanoate) synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PHA/PHB synthase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR010963
- InterPro:   IPR010941 [H]

Pfam domain/function: PF00561 Abhydrolase_1; PF07167 PhaC_N [H]

EC number: NA

Molecular weight: Translated: 66018; Mature: 66018

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESKSPFQDAIDNAMQFGQAWMDSFGQSAQSSIVETQAEDWAQWMRSSVEHPVNSIEQQM
CCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DWWGQQVNLFNDCIMSSPAEKETDRRFKDPAWNEQALYKYIKESYKLACNNIQASINNTE
HHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCEEECCCCC
GLDDETRQRLSFFSRQYLNAMSPSNFVATNPEIMKLTIESKGQNLIKGLEQLQQDLEQSV
CCCHHHHHHHHHHHHHHHHHCCCCCEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHHH
DTLNIRMTDKTAFTVGKNIATTPGKVVFKNDLFELIQYQATTEQVYKRPLLVVPPFVNKF
HEEEEEEECCCCEEECCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCEEECCCCCEE
YIMDLSPERSYTQWLVSQGHTVFMISWVNPNAEMAATDFGDYVTQGVILALDAIEAETGE
EEEECCCCHHHHHHHHHCCCEEEEEEEECCCCCEEHHHHHHHHHHHHHEEEEHHHCCCCC
REVNGIGYCIGGTLLTAAMAYLAGKRRKQRVKSATLLTTILDFGQPGELGVFINDPLISS
CHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHH
IEAQNNARGYMDGRQMAVSFSLLRENSLYWNYYVTNYLKGESPVAFDLLHWNCDNTNITA
HHCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEEEEEEECCCCCEEEEEEEEECCCCEEEE
ATHNQILRQMYLENKLKEPGGITVDGVKVDLSKVKSPCYFLSAIEDHIAVWEGTFRGTEL
HHHHHHHHHHHHHHHCCCCCCEEEECEEEEHHHCCCCHHHHHHHHHHHHHHCCCCCCCEE
LNGDNTFVLAESGHIAGPMNPPSSNKYGFWTNSDNAQSPAKWLAEADNHSGSWWPHWQSW
ECCCCEEEEEECCCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHCCCCCCCCCCCHHHH
VDERNFSDKIAARSLTGKLDAPGEYVKQRIEDVIAPKEEVRHDT
HHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHCCCC
>Mature Secondary Structure
MESKSPFQDAIDNAMQFGQAWMDSFGQSAQSSIVETQAEDWAQWMRSSVEHPVNSIEQQM
CCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DWWGQQVNLFNDCIMSSPAEKETDRRFKDPAWNEQALYKYIKESYKLACNNIQASINNTE
HHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCEEECCCCC
GLDDETRQRLSFFSRQYLNAMSPSNFVATNPEIMKLTIESKGQNLIKGLEQLQQDLEQSV
CCCHHHHHHHHHHHHHHHHHCCCCCEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHHH
DTLNIRMTDKTAFTVGKNIATTPGKVVFKNDLFELIQYQATTEQVYKRPLLVVPPFVNKF
HEEEEEEECCCCEEECCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCEEECCCCCEE
YIMDLSPERSYTQWLVSQGHTVFMISWVNPNAEMAATDFGDYVTQGVILALDAIEAETGE
EEEECCCCHHHHHHHHHCCCEEEEEEEECCCCCEEHHHHHHHHHHHHHEEEEHHHCCCCC
REVNGIGYCIGGTLLTAAMAYLAGKRRKQRVKSATLLTTILDFGQPGELGVFINDPLISS
CHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHH
IEAQNNARGYMDGRQMAVSFSLLRENSLYWNYYVTNYLKGESPVAFDLLHWNCDNTNITA
HHCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEEEEEEECCCCCEEEEEEEEECCCCEEEE
ATHNQILRQMYLENKLKEPGGITVDGVKVDLSKVKSPCYFLSAIEDHIAVWEGTFRGTEL
HHHHHHHHHHHHHHHCCCCCCEEEECEEEEHHHCCCCHHHHHHHHHHHHHHCCCCCCCEE
LNGDNTFVLAESGHIAGPMNPPSSNKYGFWTNSDNAQSPAKWLAEADNHSGSWWPHWQSW
ECCCCEEEEEECCCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHCCCCCCCCCCCHHHH
VDERNFSDKIAARSLTGKLDAPGEYVKQRIEDVIAPKEEVRHDT
HHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7582015; 9734305; 11481430 [H]