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The map label for this gene is phnE [H]

Identifier: 157961573

GI number: 157961573

Start: 2126158

End: 2127687

Strand: Direct

Name: phnE [H]

Synonym: Spea_1749

Alternate gene names: 157961573

Gene position: 2126158-2127687 (Clockwise)

Preceding gene: 157961572

Following gene: 157961575

Centisome position: 41.09

GC content: 46.21

Gene sequence:

>1530_bases
ATGCCACGCCTATTTCCTTTTGTAGGTTACTGGAAAAAAGTGACCTTAGCGCTGTGGGCTGTTTCGCTGCTGTGTTTTTA
TTTTGCCGATACCGAGGTGATAGCACTCGATCCATGGAGTGAATTGTTACTGATAGGCAAAGGCTTTATTGCGCCCGATT
TTTTTGCTACCGAATACCTGATTGAAGCGCTGTGGCAAACCGTGAGTTTTGCACTATTGGGAGTGATTATCGCATTGGTG
TGTGGGGCGCCACTGGCTTTGGTGTATCACAAACCCTTTATCGCTGCGATGTGTTCGTTTATTCGCTCAATTCATGAGAT
CTTCTGGGCGTTACTTTTTCTACAGATCTTTGGCTTGTCACCACTTACTGGGATCTTAGCTATCGCACTGCCTTATGGCG
CGACGTTTGCGCGAGTATTCAGTGATATCTTGCAGCAAGCGCCTACAACAACATTGCAAACTTTACCTTATCGAACCGAC
CGAATTAGCCGTTATCTCTATGGAAGACTGGCCCATGTGTTGGTGCCCGTACGCGCCTATGTGCGCTATCGGTTTGAGTG
CGCATTAAGAAGCAGTGCTGTGCTGGGCTTTGTGGGGATGCCAACCTTAGGTTTTTATTTAGAGACGGCTTTTCGCCAAG
GCCATTATCACCAAGGCGCAGCGCTGCTGATGCTGTTTATCGCCTTAATTGGTACCATCCCTTATTGGTGTAGAGTCAAG
GCGTTACCGGTTTATTTTGTACTGGCAATATGGGCGCTGCCTGAGATGCCAAATATCGATGGCGCATTGATTTGGCGTTT
CTTAAGCCAAGATATTTTACCGCCAATGTTTCAAAACCAAATGTTCCAAAGCGAGATGTTGCAAGCCAGTACAATGAGTA
GCAGCGAGGTGTTTAGCCAGTTCTTTGATTGGTTAAGCAAGCTACTAACAGATCAAGCGCTACCAGGAATTATTGCGACC
TTATTGTTAGCGCTAGCGGCTCTCGGGTTGAGTCATGTTTTAGCCCTAATTGGCAGTGCCATTAGTGACAAGCGTCTGAG
TGGTGGCGTGGTAGCGAGCGTAAATCGTTTGTTGTTACTTATTTTAAGGTCGGTGCCTGAGTATATCTTGGCCTTCGTGT
TTATGTTGCTGCTCGGTCCGTCGATGTTACCAGCCATATTGGCATTAGCCATCCATAATGGCGCGCTGATAGCGTATCTC
AGCATTAGGCATGCCGATAACGTGGCTTTCTCAGCACATTTTACTCATCGAATCGACGGATATGGATTCGAAGTTATGCC
AGCCATATATCCGAATATGATGGCACTGCTGTTTTATCGCTTTGAGGTGATTTTAAGAGAGACTGCTATTTTGGGGATGC
TTGGCATTGCCACTCTGGGATTTTATATCGACAGTAATTTTGCGGAAATTCGTTTTAACGGCGCCTTAGTGCTGATGTGT
GTTACCGCTTTAGTGAATATATTGGTCGACATTTTGAGTCGGCGGTTACTGTATTCAAAAGACAAAAGCCAGCTAGGCTG
CGCACATTAG

Upstream 100 bases:

>100_bases
GTGGGCGACAAGCGTGTTAATGAAAAGTCTGAATCTCCCCAAATAGAGCCACTTAACAGCGTCGTTAAGCGTTATTAGTT
TGCCAATACACCCCCCCGTA

Downstream 100 bases:

>100_bases
TCACGTCAGCCACAGATTCATTATCTCGCTTTCGAAGCACACGAACCGCTTTATGAAGCGCAAACGGTGTCCATTTAAGA
AGACTGACTAGCTAAAAATT

Product: binding-protein-dependent transport systems inner membrane component

Products: 3-phospho-serine [Cytoplasm]; phosphite [Cytoplasm]; ADP; phosphate [Cytoplasm]; alkylphosphonate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 509; Mature: 508

Protein sequence:

>509_residues
MPRLFPFVGYWKKVTLALWAVSLLCFYFADTEVIALDPWSELLLIGKGFIAPDFFATEYLIEALWQTVSFALLGVIIALV
CGAPLALVYHKPFIAAMCSFIRSIHEIFWALLFLQIFGLSPLTGILAIALPYGATFARVFSDILQQAPTTTLQTLPYRTD
RISRYLYGRLAHVLVPVRAYVRYRFECALRSSAVLGFVGMPTLGFYLETAFRQGHYHQGAALLMLFIALIGTIPYWCRVK
ALPVYFVLAIWALPEMPNIDGALIWRFLSQDILPPMFQNQMFQSEMLQASTMSSSEVFSQFFDWLSKLLTDQALPGIIAT
LLLALAALGLSHVLALIGSAISDKRLSGGVVASVNRLLLLILRSVPEYILAFVFMLLLGPSMLPAILALAIHNGALIAYL
SIRHADNVAFSAHFTHRIDGYGFEVMPAIYPNMMALLFYRFEVILRETAILGMLGIATLGFYIDSNFAEIRFNGALVLMC
VTALVNILVDILSRRLLYSKDKSQLGCAH

Sequences:

>Translated_509_residues
MPRLFPFVGYWKKVTLALWAVSLLCFYFADTEVIALDPWSELLLIGKGFIAPDFFATEYLIEALWQTVSFALLGVIIALV
CGAPLALVYHKPFIAAMCSFIRSIHEIFWALLFLQIFGLSPLTGILAIALPYGATFARVFSDILQQAPTTTLQTLPYRTD
RISRYLYGRLAHVLVPVRAYVRYRFECALRSSAVLGFVGMPTLGFYLETAFRQGHYHQGAALLMLFIALIGTIPYWCRVK
ALPVYFVLAIWALPEMPNIDGALIWRFLSQDILPPMFQNQMFQSEMLQASTMSSSEVFSQFFDWLSKLLTDQALPGIIAT
LLLALAALGLSHVLALIGSAISDKRLSGGVVASVNRLLLLILRSVPEYILAFVFMLLLGPSMLPAILALAIHNGALIAYL
SIRHADNVAFSAHFTHRIDGYGFEVMPAIYPNMMALLFYRFEVILRETAILGMLGIATLGFYIDSNFAEIRFNGALVLMC
VTALVNILVDILSRRLLYSKDKSQLGCAH
>Mature_508_residues
PRLFPFVGYWKKVTLALWAVSLLCFYFADTEVIALDPWSELLLIGKGFIAPDFFATEYLIEALWQTVSFALLGVIIALVC
GAPLALVYHKPFIAAMCSFIRSIHEIFWALLFLQIFGLSPLTGILAIALPYGATFARVFSDILQQAPTTTLQTLPYRTDR
ISRYLYGRLAHVLVPVRAYVRYRFECALRSSAVLGFVGMPTLGFYLETAFRQGHYHQGAALLMLFIALIGTIPYWCRVKA
LPVYFVLAIWALPEMPNIDGALIWRFLSQDILPPMFQNQMFQSEMLQASTMSSSEVFSQFFDWLSKLLTDQALPGIIATL
LLALAALGLSHVLALIGSAISDKRLSGGVVASVNRLLLLILRSVPEYILAFVFMLLLGPSMLPAILALAIHNGALIAYLS
IRHADNVAFSAHFTHRIDGYGFEVMPAIYPNMMALLFYRFEVILRETAILGMLGIATLGFYIDSNFAEIRFNGALVLMCV
TALVNILVDILSRRLLYSKDKSQLGCAH

Specific function: Part of the binding-protein-dependent transport system for phosphonates; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG3639

COG function: function code P; ABC-type phosphate/phosphonate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR005769 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 56757; Mature: 56626

Theoretical pI: Translated: 8.26; Mature: 8.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRLFPFVGYWKKVTLALWAVSLLCFYFADTEVIALDPWSELLLIGKGFIAPDFFATEYL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHCCCCCCHHHHHHHH
IEALWQTVSFALLGVIIALVCGAPLALVYHKPFIAAMCSFIRSIHEIFWALLFLQIFGLS
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PLTGILAIALPYGATFARVFSDILQQAPTTTLQTLPYRTDRISRYLYGRLAHVLVPVRAY
HHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
VRYRFECALRSSAVLGFVGMPTLGFYLETAFRQGHYHQGAALLMLFIALIGTIPYWCRVK
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHHH
ALPVYFVLAIWALPEMPNIDGALIWRFLSQDILPPMFQNQMFQSEMLQASTMSSSEVFSQ
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHH
FFDWLSKLLTDQALPGIIATLLLALAALGLSHVLALIGSAISDKRLSGGVVASVNRLLLL
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
ILRSVPEYILAFVFMLLLGPSMLPAILALAIHNGALIAYLSIRHADNVAFSAHFTHRIDG
HHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCEEEEEEEECCCCCEEEEEHHHHCCC
YGFEVMPAIYPNMMALLFYRFEVILRETAILGMLGIATLGFYIDSNFAEIRFNGALVLMC
CCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHH
VTALVNILVDILSRRLLYSKDKSQLGCAH
HHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
PRLFPFVGYWKKVTLALWAVSLLCFYFADTEVIALDPWSELLLIGKGFIAPDFFATEYL
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHCCCCCCHHHHHHHH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HHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; 3-phospho-serine [Periplasm]; phosphite [Periplasm]; H2O; phosphate [Periplasm]; alkylphosphonate [Periplasm] [C]

Specific reaction: H2O + ATP + 3-phospho-serine [Periplasm] = ADP + phosphate + 3-phospho-serine [Cytoplasm] ATP + phosphite [Periplasm] + H2O = phosphite [Cytoplasm] + ADP + phosphate phosphate [Periplasm] + ATP + H2O = ADP + phosphate [Cytoplasm] + phosphate [Cytoplasm] A

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2155230; 1840580; 7610040; 9278503 [H]