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The map label for this gene is gshA

Identifier: 157961036

GI number: 157961036

Start: 1472008

End: 1473576

Strand: Direct

Name: gshA

Synonym: Spea_1208

Alternate gene names: 157961036

Gene position: 1472008-1473576 (Clockwise)

Preceding gene: 157961035

Following gene: 157961037

Centisome position: 28.45

GC content: 43.59

Gene sequence:

>1569_bases
TTGAAATCTTTCAATGAATTAGTTGGTAAGTTGTCGGATCCGGCAAGTCGGCAAGCGCTAAAATCGATGCAGCGAGGCAT
TGAGCGTGAAGCGTTAAGAATTGAAAAGTCGGGTCACTTGGCATTGGATAAACATCCTAAAGCACTGGGCTCGGCATTAA
CGCATTCGCGGATCACTACAGATTATAGTGAATCTCTACTTGAGTTTATTACGCCTGTTTTTGCTGATATCGATGAATTA
GTTGAAGATCTCACTCTGACTCATGCTTATAGTGTGCGTCACCTCAACGGCCAACGCTTATGGCCTGTGAGTATGCCTTG
TTATCTTGGCGATGGTGCTGATATTCCTATCGCCGATTATGGTAGCTCTAATTCAGGTCAAATGAAGAGTCTGTATCGTA
AAGGTCTAACTTATCGATATGGCGCTCAGATGCAGATTATCTCAGGGGTGCACTTTAATTTCTCTGTATCAGATAAGCTT
TGGCACAGATTGTATGAGCTTTCAGATAAGTCATTAAGTTTAGATGATTTTATTTCGGATTCCTATTTTGGACTTATCCG
TAACTACCGTCGTCTAGTCTGGGTATTACCTTACCTGTTTGGTGCGTCACCAGCGCTGTGCAGCACCTTTATTCAAGACC
CAAAGACCAGTAATTTCCCGTTTGAAGTGATTGGCGATGGTACCTTGTTTATGCCTTACGCAACGTCATTGCGTATGAGT
GATTTAGGCTATACCAATCAAGAGCAAGATAACTTAAATATTAGTTACAACAGTCTCACCGATTATTTAACGGGCATGCA
AGCCGCGATAAATATGCCGTCGGCAAACTTTGCAAAAATTGGCGTTAAAGTTGATGGTGAATACCGTCAGTTGAACGACA
ATATCTTACAGATAGAAAACGAATTTTACTCTCCAATTCGCGCCAAACGAGTGGCTAAGGGCTCTGAAAAGCCATCTGAG
TCGCTAGCACGCGCAGGGGTTGAGTATATTGAAGTTAGAGCGTTAGACGTTAACCCATACAGCGCCGTAGGCATTGAAAA
GTCGCAGATCCGCTTCTTAGATCTCTTCCTATTGAACTGTTTACTGCAACCATCGGCACCGTCAGATGCAACTGAAGAGG
CTGAAATTGCCGAAAACTTGCAAGCAGTTGTTCTAGAAGGGCGTAAGCCAGGCTTGCTGCTAAAGCGCTCTGGTCAAGAG
TTAAGCTTAACTCATTGGCTTGAGTCGTTATTTGGTAATCTAAAGCAGATAGCCCAACTGCTAGACGATGAAGGTCAAGA
TGACTATCAAGTGGCACTCGCTAAATGGCATAAAGCAGTCGAAAATCCTAATGAAACTCTGTCGGGTAAAATCATGGCAG
GGCTAAAGGATGAGCAGGTTGATCATAGTGAGTGGGTGATGGAGCTTGCAGAGCAGTATCATCAGCAGTTAAAAGCTTAC
CCGCTAACAGAGAATGTAGACAAACGATATCAGCAAGATGCTGTCGAGTCGCTAGAGAAACAGGCGTATCTAGAGTCGCA
ACCTAGCGTCAGCTTCGATCAGTTTTTAGTCGATTACTTTAAGGTTTAG

Upstream 100 bases:

>100_bases
TTGATAAACGCTAATTTGGTACGACTTGAAATTTAAGTAAGTTATAGCGGGTCTACCAGACAAGTTATAAGGTTAGAGTC
ACGCACTAGAGAATAATGTA

Downstream 100 bases:

>100_bases
TTTTGGCGATAAAGGGCTTCAAAAGCGTATTAGGCATAGCTGCATTATTATTGATTGTATCGGGTTGTGCTTCACAATCC
GATACCGCTAATCAGTGTGG

Product: glutamate--cysteine ligase

Products: NA

Alternate protein names: Gamma-ECS; GCS; Gamma-glutamylcysteine synthetase

Number of amino acids: Translated: 522; Mature: 522

Protein sequence:

>522_residues
MKSFNELVGKLSDPASRQALKSMQRGIEREALRIEKSGHLALDKHPKALGSALTHSRITTDYSESLLEFITPVFADIDEL
VEDLTLTHAYSVRHLNGQRLWPVSMPCYLGDGADIPIADYGSSNSGQMKSLYRKGLTYRYGAQMQIISGVHFNFSVSDKL
WHRLYELSDKSLSLDDFISDSYFGLIRNYRRLVWVLPYLFGASPALCSTFIQDPKTSNFPFEVIGDGTLFMPYATSLRMS
DLGYTNQEQDNLNISYNSLTDYLTGMQAAINMPSANFAKIGVKVDGEYRQLNDNILQIENEFYSPIRAKRVAKGSEKPSE
SLARAGVEYIEVRALDVNPYSAVGIEKSQIRFLDLFLLNCLLQPSAPSDATEEAEIAENLQAVVLEGRKPGLLLKRSGQE
LSLTHWLESLFGNLKQIAQLLDDEGQDDYQVALAKWHKAVENPNETLSGKIMAGLKDEQVDHSEWVMELAEQYHQQLKAY
PLTENVDKRYQQDAVESLEKQAYLESQPSVSFDQFLVDYFKV

Sequences:

>Translated_522_residues
MKSFNELVGKLSDPASRQALKSMQRGIEREALRIEKSGHLALDKHPKALGSALTHSRITTDYSESLLEFITPVFADIDEL
VEDLTLTHAYSVRHLNGQRLWPVSMPCYLGDGADIPIADYGSSNSGQMKSLYRKGLTYRYGAQMQIISGVHFNFSVSDKL
WHRLYELSDKSLSLDDFISDSYFGLIRNYRRLVWVLPYLFGASPALCSTFIQDPKTSNFPFEVIGDGTLFMPYATSLRMS
DLGYTNQEQDNLNISYNSLTDYLTGMQAAINMPSANFAKIGVKVDGEYRQLNDNILQIENEFYSPIRAKRVAKGSEKPSE
SLARAGVEYIEVRALDVNPYSAVGIEKSQIRFLDLFLLNCLLQPSAPSDATEEAEIAENLQAVVLEGRKPGLLLKRSGQE
LSLTHWLESLFGNLKQIAQLLDDEGQDDYQVALAKWHKAVENPNETLSGKIMAGLKDEQVDHSEWVMELAEQYHQQLKAY
PLTENVDKRYQQDAVESLEKQAYLESQPSVSFDQFLVDYFKV
>Mature_522_residues
MKSFNELVGKLSDPASRQALKSMQRGIEREALRIEKSGHLALDKHPKALGSALTHSRITTDYSESLLEFITPVFADIDEL
VEDLTLTHAYSVRHLNGQRLWPVSMPCYLGDGADIPIADYGSSNSGQMKSLYRKGLTYRYGAQMQIISGVHFNFSVSDKL
WHRLYELSDKSLSLDDFISDSYFGLIRNYRRLVWVLPYLFGASPALCSTFIQDPKTSNFPFEVIGDGTLFMPYATSLRMS
DLGYTNQEQDNLNISYNSLTDYLTGMQAAINMPSANFAKIGVKVDGEYRQLNDNILQIENEFYSPIRAKRVAKGSEKPSE
SLARAGVEYIEVRALDVNPYSAVGIEKSQIRFLDLFLLNCLLQPSAPSDATEEAEIAENLQAVVLEGRKPGLLLKRSGQE
LSLTHWLESLFGNLKQIAQLLDDEGQDDYQVALAKWHKAVENPNETLSGKIMAGLKDEQVDHSEWVMELAEQYHQQLKAY
PLTENVDKRYQQDAVESLEKQAYLESQPSVSFDQFLVDYFKV

Specific function: Glutathione biosynthesis; first step. [C]

COG id: COG2918

COG function: function code H; Gamma-glutamylcysteine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate--cysteine ligase type 1 family. Type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1789044, Length=503, Percent_Identity=45.5268389662028, Blast_Score=448, Evalue=1e-127,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GSH1_SHEPA (A8H1U8)

Other databases:

- EMBL:   CP000851
- RefSeq:   YP_001501070.1
- ProteinModelPortal:   A8H1U8
- SMR:   A8H1U8
- STRING:   A8H1U8
- GeneID:   5661607
- GenomeReviews:   CP000851_GR
- KEGG:   spl:Spea_1208
- NMPDR:   fig|398579.3.peg.1055
- eggNOG:   COG2918
- HOGENOM:   HBG289023
- OMA:   FGLIRNY
- ProtClustDB:   PRK02107
- BioCyc:   SPEA398579:SPEA_1208-MONOMER
- HAMAP:   MF_00578
- InterPro:   IPR007370
- InterPro:   IPR006334
- TIGRFAMs:   TIGR01434

Pfam domain/function: PF04262 Glu_cys_ligase

EC number: =6.3.2.2

Molecular weight: Translated: 58929; Mature: 58929

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSFNELVGKLSDPASRQALKSMQRGIEREALRIEKSGHLALDKHPKALGSALTHSRITT
CCCHHHHHHHCCCHHHHHHHHHHHHCHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHCCC
DYSESLLEFITPVFADIDELVEDLTLTHAYSVRHLNGQRLWPVSMPCYLGDGADIPIADY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEEEECCCEEECCCCCCCEECC
GSSNSGQMKSLYRKGLTYRYGAQMQIISGVHFNFSVSDKLWHRLYELSDKSLSLDDFISD
CCCCCHHHHHHHHCCCCEECCCCEEEEECEEEEEECHHHHHHHHHHHCCCCCCHHHHHHH
SYFGLIRNYRRLVWVLPYLFGASPALCSTFIQDPKTSNFPFEVIGDGTLFMPYATSLRMS
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCEEEECCCEEEECCCCCEEHH
DLGYTNQEQDNLNISYNSLTDYLTGMQAAINMPSANFAKIGVKVDGEYRQLNDNILQIEN
HCCCCCCCCCCCCEEHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCHHHHCCCCEEECH
EFYSPIRAKRVAKGSEKPSESLARAGVEYIEVRALDVNPYSAVGIEKSQIRFLDLFLLNC
HHHCHHHHHHHHCCCCCCHHHHHHCCCEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHH
LLQPSAPSDATEEAEIAENLQAVVLEGRKPGLLLKRSGQELSLTHWLESLFGNLKQIAQL
HCCCCCCCCCCHHHHHHHHHHHHEECCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHH
LDDEGQDDYQVALAKWHKAVENPNETLSGKIMAGLKDEQVDHSEWVMELAEQYHQQLKAY
HCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHCC
PLTENVDKRYQQDAVESLEKQAYLESQPSVSFDQFLVDYFKV
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MKSFNELVGKLSDPASRQALKSMQRGIEREALRIEKSGHLALDKHPKALGSALTHSRITT
CCCHHHHHHHCCCHHHHHHHHHHHHCHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHCCC
DYSESLLEFITPVFADIDELVEDLTLTHAYSVRHLNGQRLWPVSMPCYLGDGADIPIADY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEEEECCCEEECCCCCCCEECC
GSSNSGQMKSLYRKGLTYRYGAQMQIISGVHFNFSVSDKLWHRLYELSDKSLSLDDFISD
CCCCCHHHHHHHHCCCCEECCCCEEEEECEEEEEECHHHHHHHHHHHCCCCCCHHHHHHH
SYFGLIRNYRRLVWVLPYLFGASPALCSTFIQDPKTSNFPFEVIGDGTLFMPYATSLRMS
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCEEEECCCEEEECCCCCEEHH
DLGYTNQEQDNLNISYNSLTDYLTGMQAAINMPSANFAKIGVKVDGEYRQLNDNILQIEN
HCCCCCCCCCCCCEEHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCHHHHCCCCEEECH
EFYSPIRAKRVAKGSEKPSESLARAGVEYIEVRALDVNPYSAVGIEKSQIRFLDLFLLNC
HHHCHHHHHHHHCCCCCCHHHHHHCCCEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHH
LLQPSAPSDATEEAEIAENLQAVVLEGRKPGLLLKRSGQELSLTHWLESLFGNLKQIAQL
HCCCCCCCCCCHHHHHHHHHHHHEECCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHH
LDDEGQDDYQVALAKWHKAVENPNETLSGKIMAGLKDEQVDHSEWVMELAEQYHQQLKAY
HCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHCC
PLTENVDKRYQQDAVESLEKQAYLESQPSVSFDQFLVDYFKV
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA