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The map label for this gene is pcm

Identifier: 157961019

GI number: 157961019

Start: 1445346

End: 1445981

Strand: Direct

Name: pcm

Synonym: Spea_1191

Alternate gene names: 157961019

Gene position: 1445346-1445981 (Clockwise)

Preceding gene: 157961018

Following gene: 157961020

Centisome position: 27.93

GC content: 47.48

Gene sequence:

>636_bases
ATGAACCCTGTCGCCTCAACATCAGCATTGAACCTTGCAAGAAGTCTGTATGAGGCTGGGATCCGTAATGAAGCTGTATT
GCGTGCGGTCGCAAATACTCCGAGGGAGCAGTTTCTTGAACCTGCACTTGGTCATAAAGCTTATGAGAATACTGCGCTGC
CGATAGGGCAAGGTCAGACTATTTCTCAGCCTTATATCGTTGCCAGAATGACTGAAATTATCTTGCAGAATAAGCCAAGT
AAAGTGCTTGAGATAGGTACGGGATCAGGCTATCAAGCTGCGGTTTTAGCACAGCTAGTTCCACAACTTTGCACGGTGGA
ACGAATTAAAAGTTTACAGATCCAGGCCAGACAGAGGCTTAAAAAGCTCGATTTACATAATGTCGCTTTCAAATATGGCG
ATGGTTGGCAAGGATGGCCAAGTAAAGGCCCTTACGACGCGATTATGGTGACTGCAGCTGCAAGTTCAGTTCCTGAAGCC
TTAGTGGCGCAATTAGCTGACGGTGGTGTATTAGTCATTCCTGTTGGCGAGTTATCGCAACAGCTGTTGAAGCTTACCCG
AGTTGGCAATCAGTTTACCTCCGAGGTGATTGAGAATGTGCGCTTTGTGCCGCTAGTGAGTGGCGAGCTGGCATAG

Upstream 100 bases:

>100_bases
ATCAGCGTTTAACTGTCGAGTGAAAACCTTTATAGATTGCCCTATAGAAACGGGCAATCGAAGCGTTAACAAATTTATTG
AAATATTGGAAAAGAGATTT

Downstream 100 bases:

>100_bases
GCATTTTTACACGGGCTTATCGAGCCAGCAATCAAGTAATAATTAAATAGTGTCGACAACAGGGTAATTTGGGGTGAAGA
GTTAACAATGAGGTTCGTTT

Product: protein-L-isoaspartate O-methyltransferase

Products: NA

Alternate protein names: L-isoaspartyl protein carboxyl methyltransferase; Protein L-isoaspartyl methyltransferase; Protein-beta-aspartate methyltransferase; PIMT

Number of amino acids: Translated: 211; Mature: 211

Protein sequence:

>211_residues
MNPVASTSALNLARSLYEAGIRNEAVLRAVANTPREQFLEPALGHKAYENTALPIGQGQTISQPYIVARMTEIILQNKPS
KVLEIGTGSGYQAAVLAQLVPQLCTVERIKSLQIQARQRLKKLDLHNVAFKYGDGWQGWPSKGPYDAIMVTAAASSVPEA
LVAQLADGGVLVIPVGELSQQLLKLTRVGNQFTSEVIENVRFVPLVSGELA

Sequences:

>Translated_211_residues
MNPVASTSALNLARSLYEAGIRNEAVLRAVANTPREQFLEPALGHKAYENTALPIGQGQTISQPYIVARMTEIILQNKPS
KVLEIGTGSGYQAAVLAQLVPQLCTVERIKSLQIQARQRLKKLDLHNVAFKYGDGWQGWPSKGPYDAIMVTAAASSVPEA
LVAQLADGGVLVIPVGELSQQLLKLTRVGNQFTSEVIENVRFVPLVSGELA
>Mature_211_residues
MNPVASTSALNLARSLYEAGIRNEAVLRAVANTPREQFLEPALGHKAYENTALPIGQGQTISQPYIVARMTEIILQNKPS
KVLEIGTGSGYQAAVLAQLVPQLCTVERIKSLQIQARQRLKKLDLHNVAFKYGDGWQGWPSKGPYDAIMVTAAASSVPEA
LVAQLADGGVLVIPVGELSQQLLKLTRVGNQFTSEVIENVRFVPLVSGELA

Specific function: Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins

COG id: COG2518

COG function: function code O; Protein-L-isoaspartate carboxylmethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family

Homologues:

Organism=Homo sapiens, GI226530908, Length=206, Percent_Identity=30.5825242718447, Blast_Score=77, Evalue=8e-15,
Organism=Escherichia coli, GI1789100, Length=200, Percent_Identity=59.5, Blast_Score=248, Evalue=2e-67,
Organism=Caenorhabditis elegans, GI71983477, Length=175, Percent_Identity=34.8571428571429, Blast_Score=94, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI193207222, Length=140, Percent_Identity=35.7142857142857, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI17981723, Length=216, Percent_Identity=30.5555555555556, Blast_Score=82, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PIMT_SHEPA (A8H1T1)

Other databases:

- EMBL:   CP000851
- RefSeq:   YP_001501053.1
- ProteinModelPortal:   A8H1T1
- SMR:   A8H1T1
- STRING:   A8H1T1
- GeneID:   5661590
- GenomeReviews:   CP000851_GR
- KEGG:   spl:Spea_1191
- NMPDR:   fig|398579.3.peg.1040
- eggNOG:   COG2518
- HOGENOM:   HBG699907
- OMA:   MEEVPRE
- ProtClustDB:   CLSK907169
- BioCyc:   SPEA398579:SPEA_1191-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00090
- InterPro:   IPR000682
- PANTHER:   PTHR11579
- TIGRFAMs:   TIGR00080

Pfam domain/function: PF01135 PCMT

EC number: =2.1.1.77

Molecular weight: Translated: 22758; Mature: 22758

Theoretical pI: Translated: 8.97; Mature: 8.97

Prosite motif: PS01279 PCMT

Important sites: ACT_SITE 62-62

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPVASTSALNLARSLYEAGIRNEAVLRAVANTPREQFLEPALGHKAYENTALPIGQGQT
CCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHCCHHHCCCCCCCCCCCC
ISQPYIVARMTEIILQNKPSKVLEIGTGSGYQAAVLAQLVPQLCTVERIKSLQIQARQRL
CCCCHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KKLDLHNVAFKYGDGWQGWPSKGPYDAIMVTAAASSVPEALVAQLADGGVLVIPVGELSQ
HHHHHHHHHEEECCCCCCCCCCCCHHHEEEEHHHHHHHHHHHHHHCCCCEEEEEHHHHHH
QLLKLTRVGNQFTSEVIENVRFVPLVSGELA
HHHHHHHHCHHHHHHHHHCCEEEEECCCCCC
>Mature Secondary Structure
MNPVASTSALNLARSLYEAGIRNEAVLRAVANTPREQFLEPALGHKAYENTALPIGQGQT
CCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHCCHHHCCCCCCCCCCCC
ISQPYIVARMTEIILQNKPSKVLEIGTGSGYQAAVLAQLVPQLCTVERIKSLQIQARQRL
CCCCHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KKLDLHNVAFKYGDGWQGWPSKGPYDAIMVTAAASSVPEALVAQLADGGVLVIPVGELSQ
HHHHHHHHHEEECCCCCCCCCCCCHHHEEEEHHHHHHHHHHHHHHCCCCEEEEEHHHHHH
QLLKLTRVGNQFTSEVIENVRFVPLVSGELA
HHHHHHHHCHHHHHHHHHCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA