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The map label for this gene is pyrG [H]

Identifier: 157961012

GI number: 157961012

Start: 1438230

End: 1439870

Strand: Direct

Name: pyrG [H]

Synonym: Spea_1184

Alternate gene names: 157961012

Gene position: 1438230-1439870 (Clockwise)

Preceding gene: 157961011

Following gene: 157961013

Centisome position: 27.79

GC content: 45.16

Gene sequence:

>1641_bases
ATGACTACAAGGTATATCTTCGTTACTGGTGGCGTTGTTTCATCACTAGGTAAAGGCATTGCAGCAGCTTCTTTGGCTGC
AATATTAGAGGCTCGCGGCCTAAACGTAACCATTATGAAGCTGGATCCATACATTAACGTTGATCCAGGTACCATGAGTC
CAACACAGCACGGTGAAGTGTTTGTGACTGAAGACGGCGCTGAAACTGATCTAGATTTAGGTCACTATGAGCGTTTCATC
CGTACTAAGATGAATCGTCGTAATAACTTTACTACGGGTCGTATTTACGAAGAAGTACTTCGTAAAGAACGTCGTGGTGA
CTATTTAGGTGCAACCATTCAGGTTATTCCACACATCACTAACGCTATCAAGGAAAAAGTTCTTGCTGGTGGTGAAGGTC
ATGATGTGGCTATCGTTGAAATCGGCGGCACGGTAGGTGATATTGAATCTCTACCATTCCTAGAGTCTATCCGTCAGCTA
GGCGTTGAGCTTGGCCGTGATAGAACCCTATTTATGCATCTAACGCTTGTACCATTCTTGGGCGCAGCTGGCGAAGTAAA
GACTAAGCCTACTCAGCACTCTGTTAAAGAGCTACGCTCAATTGGTATCGCTCCAGATGTGTTGGTTTGTCGTGGTGATC
GCGCTATTCCTGCAAACGAGAAAGCTAAGATTTCTCTATTCTGTAACGTTGAAGAACGTGCAGTTATCTCACTAAAAGAC
GTAGACAGTATCTATAAGATCCCTGCGTTGTTGAAAGCACAAGGTCTAGACCAACTTGTGACTAAGCGTTTCGGCATCGA
TTGTAAAGAAGCCGATTTAGCCGAGTGGGAAAAGGTTGTATACCAAGAAGCAAACCCTGTGGGTGAAGTGACTATTGGTA
TGGTTGGTAAGTACATTGAACTACCAGATGCCTATAAGTCGGTTAACGAAGCATTAAAGCACGCGGGTCTATTTAACCGC
GTATCAGTTAACATTAAGTATATTGATTCACAAAACGTAGAAGCTAAGGGCGATGAAGTCTTACAAGGCTTAGACGGTAT
CTTAGTTCCTGGTGGATTCGGTGAGCGTGGTGTTGAAGGTAAGATCATGGCGGCTAAGTTCGCACGTGAAAACAACCTTC
CATATTTTGGTATCTGTTTAGGTATGCAAGTTGCGTTGATCGAGTTCGCTCGTCACGTTGCAGGTCTTGAAGGTGCGCAT
TCAACAGAATTTAAGCCAGAGACTCCACATCCAGTGGTTGGTTTGATCACTGAGTGGATCAACGAAGATGGCCAAGTTGA
AGAGCGTCATGAAGAGTCTGACCTAGGCGGTACAATGCGTCTTGGAGCCCAGCTATGTCACCTTGAAGAAGGTACTAAAG
CGGCTGCGGCTTATAAGTCTACCACTTGTGTTGAACGTCACCGTCACCGTTACGAAGTGAACAACAACTACAAAGAGCGT
CTTGAGAAAGCAGGCCTTATCTTCAGTGGCCTATCATCAGACCGTTCATTGGTTGAAATGATTGAGCTACCAAACCACCC
ATGGTTCGTAGCTGGTCAGTTCCATCCTGAATTTACATCGACTCCTCGTGATGGTCAGCCGTTATTTGAAGGGTTTGTTG
CCGCTGCAGCAGCCTACCAAAAACGTGATTTAGAAGACTAA

Upstream 100 bases:

>100_bases
AATTGTAGTTTGTCGAATCAAGCTTGCTTTGGTATAATATTGCCCCGTCCTAGTGCTTTCTTACAAGCACATATTTCCAA
CTCATTTTCTCAGGTTCAGC

Downstream 100 bases:

>100_bases
TTACGACAACGCCGCTTCCATTTAATGGGAGCGGCTTTTTTGTTTCTAGCGATGTAATAACTGCACAGAATTTCGTTTTA
CTTTTATCTTTAAATTTATC

Product: CTP synthetase

Products: NA

Alternate protein names: CTP synthetase; UTP--ammonia ligase [H]

Number of amino acids: Translated: 546; Mature: 545

Protein sequence:

>546_residues
MTTRYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPYINVDPGTMSPTQHGEVFVTEDGAETDLDLGHYERFI
RTKMNRRNNFTTGRIYEEVLRKERRGDYLGATIQVIPHITNAIKEKVLAGGEGHDVAIVEIGGTVGDIESLPFLESIRQL
GVELGRDRTLFMHLTLVPFLGAAGEVKTKPTQHSVKELRSIGIAPDVLVCRGDRAIPANEKAKISLFCNVEERAVISLKD
VDSIYKIPALLKAQGLDQLVTKRFGIDCKEADLAEWEKVVYQEANPVGEVTIGMVGKYIELPDAYKSVNEALKHAGLFNR
VSVNIKYIDSQNVEAKGDEVLQGLDGILVPGGFGERGVEGKIMAAKFARENNLPYFGICLGMQVALIEFARHVAGLEGAH
STEFKPETPHPVVGLITEWINEDGQVEERHEESDLGGTMRLGAQLCHLEEGTKAAAAYKSTTCVERHRHRYEVNNNYKER
LEKAGLIFSGLSSDRSLVEMIELPNHPWFVAGQFHPEFTSTPRDGQPLFEGFVAAAAAYQKRDLED

Sequences:

>Translated_546_residues
MTTRYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPYINVDPGTMSPTQHGEVFVTEDGAETDLDLGHYERFI
RTKMNRRNNFTTGRIYEEVLRKERRGDYLGATIQVIPHITNAIKEKVLAGGEGHDVAIVEIGGTVGDIESLPFLESIRQL
GVELGRDRTLFMHLTLVPFLGAAGEVKTKPTQHSVKELRSIGIAPDVLVCRGDRAIPANEKAKISLFCNVEERAVISLKD
VDSIYKIPALLKAQGLDQLVTKRFGIDCKEADLAEWEKVVYQEANPVGEVTIGMVGKYIELPDAYKSVNEALKHAGLFNR
VSVNIKYIDSQNVEAKGDEVLQGLDGILVPGGFGERGVEGKIMAAKFARENNLPYFGICLGMQVALIEFARHVAGLEGAH
STEFKPETPHPVVGLITEWINEDGQVEERHEESDLGGTMRLGAQLCHLEEGTKAAAAYKSTTCVERHRHRYEVNNNYKER
LEKAGLIFSGLSSDRSLVEMIELPNHPWFVAGQFHPEFTSTPRDGQPLFEGFVAAAAAYQKRDLED
>Mature_545_residues
TTRYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPYINVDPGTMSPTQHGEVFVTEDGAETDLDLGHYERFIR
TKMNRRNNFTTGRIYEEVLRKERRGDYLGATIQVIPHITNAIKEKVLAGGEGHDVAIVEIGGTVGDIESLPFLESIRQLG
VELGRDRTLFMHLTLVPFLGAAGEVKTKPTQHSVKELRSIGIAPDVLVCRGDRAIPANEKAKISLFCNVEERAVISLKDV
DSIYKIPALLKAQGLDQLVTKRFGIDCKEADLAEWEKVVYQEANPVGEVTIGMVGKYIELPDAYKSVNEALKHAGLFNRV
SVNIKYIDSQNVEAKGDEVLQGLDGILVPGGFGERGVEGKIMAAKFARENNLPYFGICLGMQVALIEFARHVAGLEGAHS
TEFKPETPHPVVGLITEWINEDGQVEERHEESDLGGTMRLGAQLCHLEEGTKAAAAYKSTTCVERHRHRYEVNNNYKERL
EKAGLIFSGLSSDRSLVEMIELPNHPWFVAGQFHPEFTSTPRDGQPLFEGFVAAAAAYQKRDLED

Specific function: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen [H]

COG id: COG0504

COG function: function code F; CTP synthase (UTP-ammonia lyase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI148491070, Length=561, Percent_Identity=44.3850267379679, Blast_Score=473, Evalue=1e-133,
Organism=Homo sapiens, GI28559085, Length=558, Percent_Identity=44.6236559139785, Blast_Score=470, Evalue=1e-132,
Organism=Homo sapiens, GI28559083, Length=558, Percent_Identity=44.6236559139785, Blast_Score=470, Evalue=1e-132,
Organism=Homo sapiens, GI221316689, Length=558, Percent_Identity=44.6236559139785, Blast_Score=470, Evalue=1e-132,
Organism=Escherichia coli, GI1789142, Length=542, Percent_Identity=77.4907749077491, Blast_Score=858, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25148299, Length=613, Percent_Identity=37.3572593800979, Blast_Score=414, Evalue=1e-116,
Organism=Saccharomyces cerevisiae, GI6319432, Length=570, Percent_Identity=40.8771929824561, Blast_Score=442, Evalue=1e-125,
Organism=Saccharomyces cerevisiae, GI6322563, Length=569, Percent_Identity=42.0035149384886, Blast_Score=440, Evalue=1e-124,
Organism=Drosophila melanogaster, GI24664469, Length=557, Percent_Identity=44.524236983842, Blast_Score=465, Evalue=1e-131,
Organism=Drosophila melanogaster, GI21357815, Length=503, Percent_Identity=43.1411530815109, Blast_Score=395, Evalue=1e-110,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004468
- InterPro:   IPR017456
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF06418 CTP_synth_N; PF00117 GATase [H]

EC number: =6.3.4.2 [H]

Molecular weight: Translated: 60082; Mature: 59951

Theoretical pI: Translated: 5.52; Mature: 5.52

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTRYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPYINVDPGTMSPTQHGEV
CCEEEEEEECHHHHHHCCHHHHHHHHHHHHHCCCEEEEEEECCEEECCCCCCCCCCCCEE
FVTEDGAETDLDLGHYERFIRTKMNRRNNFTTGRIYEEVLRKERRGDYLGATIQVIPHIT
EEECCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
NAIKEKVLAGGEGHDVAIVEIGGTVGDIESLPFLESIRQLGVELGRDRTLFMHLTLVPFL
HHHHHHHHCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEHHHHC
GAAGEVKTKPTQHSVKELRSIGIAPDVLVCRGDRAIPANEKAKISLFCNVEERAVISLKD
CCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCEEEEEEEECCCCEEEEEHH
VDSIYKIPALLKAQGLDQLVTKRFGIDCKEADLAEWEKVVYQEANPVGEVTIGMVGKYIE
HHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHCCHHC
LPDAYKSVNEALKHAGLFNRVSVNIKYIDSQNVEAKGDEVLQGLDGILVPGGFGERGVEG
CCHHHHHHHHHHHHCCCEEEEEEEEEEECCCCCCCCHHHHHHCCCCEEECCCCCCCCCCC
KIMAAKFARENNLPYFGICLGMQVALIEFARHVAGLEGAHSTEFKPETPHPVVGLITEWI
CEEEEEHHHCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHH
NEDGQVEERHEESDLGGTMRLGAQLCHLEEGTKAAAAYKSTTCVERHRHRYEVNNNYKER
CCCCCHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHH
LEKAGLIFSGLSSDRSLVEMIELPNHPWFVAGQFHPEFTSTPRDGQPLFEGFVAAAAAYQ
HHHCCCEEECCCCCHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHH
KRDLED
HCCCCC
>Mature Secondary Structure 
TTRYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPYINVDPGTMSPTQHGEV
CEEEEEEECHHHHHHCCHHHHHHHHHHHHHCCCEEEEEEECCEEECCCCCCCCCCCCEE
FVTEDGAETDLDLGHYERFIRTKMNRRNNFTTGRIYEEVLRKERRGDYLGATIQVIPHIT
EEECCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
NAIKEKVLAGGEGHDVAIVEIGGTVGDIESLPFLESIRQLGVELGRDRTLFMHLTLVPFL
HHHHHHHHCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEHHHHC
GAAGEVKTKPTQHSVKELRSIGIAPDVLVCRGDRAIPANEKAKISLFCNVEERAVISLKD
CCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCEEEEEEEECCCCEEEEEHH
VDSIYKIPALLKAQGLDQLVTKRFGIDCKEADLAEWEKVVYQEANPVGEVTIGMVGKYIE
HHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHCCHHC
LPDAYKSVNEALKHAGLFNRVSVNIKYIDSQNVEAKGDEVLQGLDGILVPGGFGERGVEG
CCHHHHHHHHHHHHCCCEEEEEEEEEEECCCCCCCCHHHHHHCCCCEEECCCCCCCCCCC
KIMAAKFARENNLPYFGICLGMQVALIEFARHVAGLEGAHSTEFKPETPHPVVGLITEWI
CEEEEEHHHCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHH
NEDGQVEERHEESDLGGTMRLGAQLCHLEEGTKAAAAYKSTTCVERHRHRYEVNNNYKER
CCCCCHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHH
LEKAGLIFSGLSSDRSLVEMIELPNHPWFVAGQFHPEFTSTPRDGQPLFEGFVAAAAAYQ
HHHCCCEEECCCCCHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHH
KRDLED
HCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA