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The map label for this gene is pilT [H]

Identifier: 157960957

GI number: 157960957

Start: 1366461

End: 1367573

Strand: Direct

Name: pilT [H]

Synonym: Spea_1129

Alternate gene names: 157960957

Gene position: 1366461-1367573 (Clockwise)

Preceding gene: 157960956

Following gene: 157960958

Centisome position: 26.41

GC content: 49.24

Gene sequence:

>1113_bases
ATGGATGTTCGTCCTTTTCTAAAGGTGATGATTGAGCGTAAGGCATCGGATCTATTTATTACCGCAGGTTTCCCACCGAG
CGCTAAAGTTGATGGTGAGCTGCGCCCCTTAGGTGAGAATGCTTTTACTCCTGCCCAGTCGTTAGATTTTGTTGAGTCAT
TGATGACTGATGTGCAAAAGCAGGAATTTCACGGGACTCGTGAGTGTAACTTTGCCTTTTCGGCTAAAGATCTTGGCCGA
TTTCGTGTCAGCGCCTTTTGGCAGCGTGAGTTTCCCGGCTGCGTGATGCGTCGAATCGAGACTCAGATCCCTGAGGTTGA
TGACTTAATGTTGCCGCCTATCTTAAAAGATTTGGTGATGAGTAAGCGCGGCTTAGTGATTTTGGTTGGTGGTACCGGTA
CAGGTAAGTCGACCTCGCTTGCGGCACTGGTCGGCTATCGAAATACCCATGCCCGCGGTCATATTCTGACGATTGAAGAT
CCGGTGGAATTTGTTCATGAGCATAAGCAGAGCATCATTACTCAGCGTGAGGTGGGGATCGATACCGAGTCTTTCGATGC
AGCGCTTAAAAGTTCTCTGCGTCAGGCGCCTGATGTGATTTTGATCGGTGAAATTCGTAGCCAGGAAACTATGGAGTTCG
CGCTGTCTTTTGCCGAGACGGGTCACCTTTGTATGGCGACTCTGCATGCTAATAACGCTAACCAAGCGCTCGACCGTATC
ATGCATTTAGTGCCGGAGAGTAAGCACCATCAACTGCTGTTTGATTTGTCGTTAAACTTACGCGGTATTGTGGCGCAGCA
ATTGGTGCCTAAGAGCGATGGCAGCGGAAGGCGTGCGGCGATTGAGGTATTGATTAATACCCCGCGTGTCGCCAGCTTGA
TTGCTAAAAATGAGCTGCATACCCTTAAAGAAACCATGGCAAAGTCAAATGAGCAGGGGATGCAGACCTTCGACCAAGCT
TTGTTAAAGCTCTACAGCGAGGGCGAGATCAGTTACGCCGATGCGCTGCATCATGCGGATTCACCAAACGACCTGCGACT
GATGATCAAGCTACAAAGCTCAGATACGACTAGCAGTAGCTCTATGGATGGGGTCACACTCAATTTAGACTAG

Upstream 100 bases:

>100_bases
AGAACTTGGTTAATCGCGGCCAAATCTCCCGTGAAGATGCCATGGCTAAGAGCTCGAATAAACAAACCAACTTTTAATAG
GTAATAGGGATAGCTAGTCT

Downstream 100 bases:

>100_bases
GCTAAGTCACCCCGATAAAAGCTGGCAATATATTCAATTGCCAGCTTTTTTTACGGCCCGTGTTGAACTCAAAGCAGATT
AAAACGTATCTGTAGATAAT

Product: twitching motility protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 370; Mature: 370

Protein sequence:

>370_residues
MDVRPFLKVMIERKASDLFITAGFPPSAKVDGELRPLGENAFTPAQSLDFVESLMTDVQKQEFHGTRECNFAFSAKDLGR
FRVSAFWQREFPGCVMRRIETQIPEVDDLMLPPILKDLVMSKRGLVILVGGTGTGKSTSLAALVGYRNTHARGHILTIED
PVEFVHEHKQSIITQREVGIDTESFDAALKSSLRQAPDVILIGEIRSQETMEFALSFAETGHLCMATLHANNANQALDRI
MHLVPESKHHQLLFDLSLNLRGIVAQQLVPKSDGSGRRAAIEVLINTPRVASLIAKNELHTLKETMAKSNEQGMQTFDQA
LLKLYSEGEISYADALHHADSPNDLRLMIKLQSSDTTSSSSMDGVTLNLD

Sequences:

>Translated_370_residues
MDVRPFLKVMIERKASDLFITAGFPPSAKVDGELRPLGENAFTPAQSLDFVESLMTDVQKQEFHGTRECNFAFSAKDLGR
FRVSAFWQREFPGCVMRRIETQIPEVDDLMLPPILKDLVMSKRGLVILVGGTGTGKSTSLAALVGYRNTHARGHILTIED
PVEFVHEHKQSIITQREVGIDTESFDAALKSSLRQAPDVILIGEIRSQETMEFALSFAETGHLCMATLHANNANQALDRI
MHLVPESKHHQLLFDLSLNLRGIVAQQLVPKSDGSGRRAAIEVLINTPRVASLIAKNELHTLKETMAKSNEQGMQTFDQA
LLKLYSEGEISYADALHHADSPNDLRLMIKLQSSDTTSSSSMDGVTLNLD
>Mature_370_residues
MDVRPFLKVMIERKASDLFITAGFPPSAKVDGELRPLGENAFTPAQSLDFVESLMTDVQKQEFHGTRECNFAFSAKDLGR
FRVSAFWQREFPGCVMRRIETQIPEVDDLMLPPILKDLVMSKRGLVILVGGTGTGKSTSLAALVGYRNTHARGHILTIED
PVEFVHEHKQSIITQREVGIDTESFDAALKSSLRQAPDVILIGEIRSQETMEFALSFAETGHLCMATLHANNANQALDRI
MHLVPESKHHQLLFDLSLNLRGIVAQQLVPKSDGSGRRAAIEVLINTPRVASLIAKNELHTLKETMAKSNEQGMQTFDQA
LLKLYSEGEISYADALHHADSPNDLRLMIKLQSSDTTSSSSMDGVTLNLD

Specific function: May be involved in pilus retraction [H]

COG id: COG5008

COG function: function code NU; Tfp pilus assembly protein, ATPase PilU

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=331, Percent_Identity=35.3474320241692, Blast_Score=215, Evalue=4e-57,
Organism=Escherichia coli, GI1789723, Length=158, Percent_Identity=41.7721518987342, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1786296, Length=373, Percent_Identity=28.686327077748, Blast_Score=97, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 40979; Mature: 40979

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDVRPFLKVMIERKASDLFITAGFPPSAKVDGELRPLGENAFTPAQSLDFVESLMTDVQK
CCCHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
QEFHGTRECNFAFSAKDLGRFRVSAFWQREFPGCVMRRIETQIPEVDDLMLPPILKDLVM
HHCCCCCCCCEEECHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHCCHHHHHHHH
SKRGLVILVGGTGTGKSTSLAALVGYRNTHARGHILTIEDPVEFVHEHKQSIITQREVGI
CCCCEEEEECCCCCCCCCCCEEEEECCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCC
DTESFDAALKSSLRQAPDVILIGEIRSQETMEFALSFAETGHLCMATLHANNANQALDRI
CHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHH
MHLVPESKHHQLLFDLSLNLRGIVAQQLVPKSDGSGRRAAIEVLINTPRVASLIAKNELH
HHHCCCCCCCEEEEEECCCHHHHHHHHHCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHH
TLKETMAKSNEQGMQTFDQALLKLYSEGEISYADALHHADSPNDLRLMIKLQSSDTTSSS
HHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCEEEEEEEECCCCCCCC
SMDGVTLNLD
CCCCEEEEEC
>Mature Secondary Structure
MDVRPFLKVMIERKASDLFITAGFPPSAKVDGELRPLGENAFTPAQSLDFVESLMTDVQK
CCCHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
QEFHGTRECNFAFSAKDLGRFRVSAFWQREFPGCVMRRIETQIPEVDDLMLPPILKDLVM
HHCCCCCCCCEEECHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHCCHHHHHHHH
SKRGLVILVGGTGTGKSTSLAALVGYRNTHARGHILTIEDPVEFVHEHKQSIITQREVGI
CCCCEEEEECCCCCCCCCCCEEEEECCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHCCC
DTESFDAALKSSLRQAPDVILIGEIRSQETMEFALSFAETGHLCMATLHANNANQALDRI
CHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHH
MHLVPESKHHQLLFDLSLNLRGIVAQQLVPKSDGSGRRAAIEVLINTPRVASLIAKNELH
HHHCCCCCCCEEEEEECCCHHHHHHHHHCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHH
TLKETMAKSNEQGMQTFDQALLKLYSEGEISYADALHHADSPNDLRLMIKLQSSDTTSSS
HHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCEEEEEEEECCCCCCCC
SMDGVTLNLD
CCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]