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The map label for this gene is pilT [H]

Identifier: 157960956

GI number: 157960956

Start: 1365400

End: 1366437

Strand: Direct

Name: pilT [H]

Synonym: Spea_1128

Alternate gene names: 157960956

Gene position: 1365400-1366437 (Clockwise)

Preceding gene: 157960946

Following gene: 157960957

Centisome position: 26.39

GC content: 47.59

Gene sequence:

>1038_bases
ATGGAAATCACAGAGTTACTTGCCTTTAGTGTAAAACACAATGCATCAGATCTACACCTTTCAGCGGGTGTTTCTCCCAT
GATCCGTGTTGATGGTGAAGTGAGAAAGATTAATTTACCCGCTTTAGATCACCAAGGTGTACATAGCTTAGTCTACGACA
TCATGAACGATAAGCAGCGTAAGGATTATGAAGAGCACCTAGAGATAGATTTCTCGTTTGAAGTGCCGGGTCTTGCACGT
TTTCGTGTTAATGCTTTTAACCAGTCTCGTGGTTCGGCGGCGGTTTTCCGTACCATTCCCAGTGACATCTTGAGTCTGGA
GCAATTAGGCGCACCGGAGATCTTCAAAAAAATCTCAAGTTTCCCCCGTGGTCTAGTATTGGTCACCGGCCCAACTGGCT
CGGGAAAGAGTACCACCTTGGCGGCAATGATCGATTATGTGAACGAAAACCGTCACGACCACATTCTGACCATTGAAGAT
CCGATAGAATTTGTTCATCAAAATAAGCAGTGCTTGATTAACCAACGTGAAGTGCATCGCCATACCCACAGCTTTAACGC
GGCACTGAGAAGTGCACTGCGTGAAGATCCAGACGTGATCTTAGTCGGTGAGATGCGAGATCTTGAAACCATTCGTCTGG
CGATGACAGCGGCGGAAACGGGTCACTTAGTCTTTGGAACCCTGCATACCACCTCTGCGGCTAAAACCATTGACCGTATT
GTCGACGTTTTCCCTGAAGGTGAGAAAGGCATGGTAAGAACCATGTTGTCTGAATCTTTGCAGGCGGTTATCTCTCAGAC
CTTGATTAAGAAAGTGGGCGGCGGCCGAGTGGCGGCGCATGAGATCATGATGGGGACGCCTGCGATTCGTAACCTTATTC
GTGAGGATAAAGTCGCGCAGATGTATTCGGCAATTCAAACGGGTATGGCCCACGGAATGCAGACGCTGGACCAATGTTTA
CAGAACTTGGTTAATCGCGGCCAAATCTCCCGTGAAGATGCCATGGCTAAGAGCTCGAATAAACAAACCAACTTTTAA

Upstream 100 bases:

>100_bases
GAATTTTGCGCCGCTTGTGCAATCCGGCTCTGGGCGATTGCGAGTCTGTCTGTTATTGTTGTCATGTTGTAAGTTTTTGG
TTGAATGGATATCCCAAACT

Downstream 100 bases:

>100_bases
TAGGTAATAGGGATAGCTAGTCTATGGATGTTCGTCCTTTTCTAAAGGTGATGATTGAGCGTAAGGCATCGGATCTATTT
ATTACCGCAGGTTTCCCACC

Product: twitching motility protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 345; Mature: 345

Protein sequence:

>345_residues
MEITELLAFSVKHNASDLHLSAGVSPMIRVDGEVRKINLPALDHQGVHSLVYDIMNDKQRKDYEEHLEIDFSFEVPGLAR
FRVNAFNQSRGSAAVFRTIPSDILSLEQLGAPEIFKKISSFPRGLVLVTGPTGSGKSTTLAAMIDYVNENRHDHILTIED
PIEFVHQNKQCLINQREVHRHTHSFNAALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFGTLHTTSAAKTIDRI
VDVFPEGEKGMVRTMLSESLQAVISQTLIKKVGGGRVAAHEIMMGTPAIRNLIREDKVAQMYSAIQTGMAHGMQTLDQCL
QNLVNRGQISREDAMAKSSNKQTNF

Sequences:

>Translated_345_residues
MEITELLAFSVKHNASDLHLSAGVSPMIRVDGEVRKINLPALDHQGVHSLVYDIMNDKQRKDYEEHLEIDFSFEVPGLAR
FRVNAFNQSRGSAAVFRTIPSDILSLEQLGAPEIFKKISSFPRGLVLVTGPTGSGKSTTLAAMIDYVNENRHDHILTIED
PIEFVHQNKQCLINQREVHRHTHSFNAALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFGTLHTTSAAKTIDRI
VDVFPEGEKGMVRTMLSESLQAVISQTLIKKVGGGRVAAHEIMMGTPAIRNLIREDKVAQMYSAIQTGMAHGMQTLDQCL
QNLVNRGQISREDAMAKSSNKQTNF
>Mature_345_residues
MEITELLAFSVKHNASDLHLSAGVSPMIRVDGEVRKINLPALDHQGVHSLVYDIMNDKQRKDYEEHLEIDFSFEVPGLAR
FRVNAFNQSRGSAAVFRTIPSDILSLEQLGAPEIFKKISSFPRGLVLVTGPTGSGKSTTLAAMIDYVNENRHDHILTIED
PIEFVHQNKQCLINQREVHRHTHSFNAALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFGTLHTTSAAKTIDRI
VDVFPEGEKGMVRTMLSESLQAVISQTLIKKVGGGRVAAHEIMMGTPAIRNLIREDKVAQMYSAIQTGMAHGMQTLDQCL
QNLVNRGQISREDAMAKSSNKQTNF

Specific function: May be involved in pilus retraction [H]

COG id: COG2805

COG function: function code NU; Tfp pilus assembly protein, pilus retraction ATPase PilT

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=329, Percent_Identity=47.4164133738602, Blast_Score=298, Evalue=3e-82,
Organism=Escherichia coli, GI1789723, Length=356, Percent_Identity=32.0224719101124, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI1786296, Length=281, Percent_Identity=32.3843416370107, Blast_Score=127, Evalue=1e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 38303; Mature: 38303

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEITELLAFSVKHNASDLHLSAGVSPMIRVDGEVRKINLPALDHQGVHSLVYDIMNDKQR
CCHHHHHHHHHCCCCCCEEEECCCCCEEEECCCEEEEECCCCCCCHHHHHHHHHHCCHHH
KDYEEHLEIDFSFEVPGLARFRVNAFNQSRGSAAVFRTIPSDILSLEQLGAPEIFKKISS
CCHHHHCEEEEEECCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHC
FPRGLVLVTGPTGSGKSTTLAAMIDYVNENRHDHILTIEDPIEFVHQNKQCLINQREVHR
CCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEECCHHHHHHCCHHHHCCHHHHHH
HTHSFNAALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFGTLHTTSAAKTIDRI
HHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHH
VDVFPEGEKGMVRTMLSESLQAVISQTLIKKVGGGRVAAHEIMMGTPAIRNLIREDKVAQ
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHH
MYSAIQTGMAHGMQTLDQCLQNLVNRGQISREDAMAKSSNKQTNF
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCC
>Mature Secondary Structure
MEITELLAFSVKHNASDLHLSAGVSPMIRVDGEVRKINLPALDHQGVHSLVYDIMNDKQR
CCHHHHHHHHHCCCCCCEEEECCCCCEEEECCCEEEEECCCCCCCHHHHHHHHHHCCHHH
KDYEEHLEIDFSFEVPGLARFRVNAFNQSRGSAAVFRTIPSDILSLEQLGAPEIFKKISS
CCHHHHCEEEEEECCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHC
FPRGLVLVTGPTGSGKSTTLAAMIDYVNENRHDHILTIEDPIEFVHQNKQCLINQREVHR
CCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEECCHHHHHHCCHHHHCCHHHHHH
HTHSFNAALRSALREDPDVILVGEMRDLETIRLAMTAAETGHLVFGTLHTTSAAKTIDRI
HHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHH
VDVFPEGEKGMVRTMLSESLQAVISQTLIKKVGGGRVAAHEIMMGTPAIRNLIREDKVAQ
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHH
MYSAIQTGMAHGMQTLDQCLQNLVNRGQISREDAMAKSSNKQTNF
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]