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The map label for this gene is yafC [C]

Identifier: 157960941

GI number: 157960941

Start: 1348137

End: 1349078

Strand: Direct

Name: yafC [C]

Synonym: Spea_1113

Alternate gene names: 157960941

Gene position: 1348137-1349078 (Clockwise)

Preceding gene: 157960940

Following gene: 157960942

Centisome position: 26.05

GC content: 45.75

Gene sequence:

>942_bases
ATGTTAGAACTTTTAGAACCAATCGCAATTTTTACCCATGTGGCTCGAGCGGGGAGTTTTAGCTCTGCAGCGAGAAAGTT
AGGCATATCAAAGTCTAAAGTCAGTACCCAAGTGGCCGATCTGGAGCACAAGCTTGGCGTTCAATTGATTCAACGTACAA
CTAGAAGCTTGAGTTTAACCGAGGCGGGTCAGCTACTGTATACCCAAGGTGAAGAGTTATTAAGAGATGCCGACCAAGCT
GTTGCTAGCGTTCATAATCTTAATGATGCTACCCGAGGCGTATTGAAAGTTGGTATTTCTCAGTCTTTCGGCACCATGCA
TATCATTCCTGCTTTGCCAGAGTTTATGGCAAGGCATCCAGAACTAGAGTTACAAGTTAGCCTGCTCGACCATAAAGTCG
ATGTGGTTAGCGAAGGTTTAGATCTGCTTCTTACTATGTCAGAGCAATTGCCCCTTGGCATGGTTGCCAGACCCTTGATG
AAATGTCAGTTCTTGTTAGCCGCCTCTCCCTCATACGTTGCAAAACATGGCGTTCCTAGCCGCCCAGAGCAGCTGGTGGA
CCATAATTGCTTGGTTTACCAAGGTGAGTGGCATGAGCATAGCGTATGGCAATTTAAACAGGGTGAAGACAACTGTGAAA
TTGGCGTAACAGGTAATTTCAGAGTCGATAATGCGCCAGCACTTAAGTCTGCTGCAGTTAGTGGTTTGGGTGTGGTTTAT
CTTGCCAGTTACCTGCTCGAAGATGAGCTTGAGAAGGGCACTTTAGTCCCTCTTCTTGAAGACTGGCAGTTGACTCATAA
TTTACCATTGCAGGCTGTTTACCCAAGACGTAAGCATTTAGCACCTAAGGTGAGCGCCTTTATCGATTTTATAAAGGATC
ATATCGGTACCCCGCCATATTGGGACGCCCCCTTAAAAGACTTGTATGCAAAGCGTAAGTAA

Upstream 100 bases:

>100_bases
TAGCGTACGCATATTCACTTAAGATATTGTTTTTATACTAAATAACATGTCTTAGCGATGGAAGTACTATTACTGTCGTA
AACACCTTAAGGCGATTTAC

Downstream 100 bases:

>100_bases
ATGGGGAAACATTGTTCTGCATTTGGGACGCTGTAAAGCTGAACGAGTGTTGGTACTTGTTACTCTTTGAAATTCAGTTA
CTTAAGCTCTGTAGCTAAAT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MLELLEPIAIFTHVARAGSFSSAARKLGISKSKVSTQVADLEHKLGVQLIQRTTRSLSLTEAGQLLYTQGEELLRDADQA
VASVHNLNDATRGVLKVGISQSFGTMHIIPALPEFMARHPELELQVSLLDHKVDVVSEGLDLLLTMSEQLPLGMVARPLM
KCQFLLAASPSYVAKHGVPSRPEQLVDHNCLVYQGEWHEHSVWQFKQGEDNCEIGVTGNFRVDNAPALKSAAVSGLGVVY
LASYLLEDELEKGTLVPLLEDWQLTHNLPLQAVYPRRKHLAPKVSAFIDFIKDHIGTPPYWDAPLKDLYAKRK

Sequences:

>Translated_313_residues
MLELLEPIAIFTHVARAGSFSSAARKLGISKSKVSTQVADLEHKLGVQLIQRTTRSLSLTEAGQLLYTQGEELLRDADQA
VASVHNLNDATRGVLKVGISQSFGTMHIIPALPEFMARHPELELQVSLLDHKVDVVSEGLDLLLTMSEQLPLGMVARPLM
KCQFLLAASPSYVAKHGVPSRPEQLVDHNCLVYQGEWHEHSVWQFKQGEDNCEIGVTGNFRVDNAPALKSAAVSGLGVVY
LASYLLEDELEKGTLVPLLEDWQLTHNLPLQAVYPRRKHLAPKVSAFIDFIKDHIGTPPYWDAPLKDLYAKRK
>Mature_313_residues
MLELLEPIAIFTHVARAGSFSSAARKLGISKSKVSTQVADLEHKLGVQLIQRTTRSLSLTEAGQLLYTQGEELLRDADQA
VASVHNLNDATRGVLKVGISQSFGTMHIIPALPEFMARHPELELQVSLLDHKVDVVSEGLDLLLTMSEQLPLGMVARPLM
KCQFLLAASPSYVAKHGVPSRPEQLVDHNCLVYQGEWHEHSVWQFKQGEDNCEIGVTGNFRVDNAPALKSAAVSGLGVVY
LASYLLEDELEKGTLVPLLEDWQLTHNLPLQAVYPRRKHLAPKVSAFIDFIKDHIGTPPYWDAPLKDLYAKRK

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=297, Percent_Identity=30.3030303030303, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI1789440, Length=299, Percent_Identity=27.7591973244147, Blast_Score=151, Evalue=6e-38,
Organism=Escherichia coli, GI1787128, Length=296, Percent_Identity=32.4324324324324, Blast_Score=150, Evalue=8e-38,
Organism=Escherichia coli, GI87081978, Length=295, Percent_Identity=30.1694915254237, Blast_Score=144, Evalue=6e-36,
Organism=Escherichia coli, GI145693193, Length=300, Percent_Identity=28.6666666666667, Blast_Score=136, Evalue=1e-33,
Organism=Escherichia coli, GI1787589, Length=285, Percent_Identity=29.1228070175439, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI1789639, Length=300, Percent_Identity=26, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1788706, Length=303, Percent_Identity=25.4125412541254, Blast_Score=88, Evalue=7e-19,
Organism=Escherichia coli, GI1790011, Length=298, Percent_Identity=24.8322147651007, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1786448, Length=289, Percent_Identity=25.9515570934256, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1789173, Length=289, Percent_Identity=24.5674740484429, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1788481, Length=305, Percent_Identity=26.8852459016393, Blast_Score=81, Evalue=9e-17,
Organism=Escherichia coli, GI157672245, Length=119, Percent_Identity=34.453781512605, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1788297, Length=303, Percent_Identity=25.4125412541254, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1789506, Length=171, Percent_Identity=28.0701754385965, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI145693105, Length=194, Percent_Identity=25.2577319587629, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1790262, Length=246, Percent_Identity=25.609756097561, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1788748, Length=121, Percent_Identity=34.7107438016529, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI2367136, Length=167, Percent_Identity=26.3473053892216, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1787879, Length=159, Percent_Identity=28.9308176100629, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34666; Mature: 34666

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLELLEPIAIFTHVARAGSFSSAARKLGISKSKVSTQVADLEHKLGVQLIQRTTRSLSLT
CCHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
EAGQLLYTQGEELLRDADQAVASVHNLNDATRGVLKVGISQSFGTMHIIPALPEFMARHP
HHCHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCEEHHHHHHHHHHCCC
ELELQVSLLDHKVDVVSEGLDLLLTMSEQLPLGMVARPLMKCQFLLAASPSYVAKHGVPS
CCEEEEHHHHHHHHHHHHCHHHEEHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHCCCCC
RPEQLVDHNCLVYQGEWHEHSVWQFKQGEDNCEIGVTGNFRVDNAPALKSAAVSGLGVVY
CHHHHHCCCEEEEECCCCCCCCEEECCCCCCCEEEECCCEEECCCCHHHHHHHHHHHHHH
LASYLLEDELEKGTLVPLLEDWQLTHNLPLQAVYPRRKHLAPKVSAFIDFIKDHIGTPPY
HHHHHHHHHHCCCCCCCHHHCCHHHCCCCCCEECCHHHHCCHHHHHHHHHHHHHCCCCCC
WDAPLKDLYAKRK
CCCCHHHHHHCCC
>Mature Secondary Structure
MLELLEPIAIFTHVARAGSFSSAARKLGISKSKVSTQVADLEHKLGVQLIQRTTRSLSLT
CCHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
EAGQLLYTQGEELLRDADQAVASVHNLNDATRGVLKVGISQSFGTMHIIPALPEFMARHP
HHCHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCEEHHHHHHHHHHCCC
ELELQVSLLDHKVDVVSEGLDLLLTMSEQLPLGMVARPLMKCQFLLAASPSYVAKHGVPS
CCEEEEHHHHHHHHHHHHCHHHEEHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHCCCCC
RPEQLVDHNCLVYQGEWHEHSVWQFKQGEDNCEIGVTGNFRVDNAPALKSAAVSGLGVVY
CHHHHHCCCEEEEECCCCCCCCEEECCCCCCCEEEECCCEEECCCCHHHHHHHHHHHHHH
LASYLLEDELEKGTLVPLLEDWQLTHNLPLQAVYPRRKHLAPKVSAFIDFIKDHIGTPPY
HHHHHHHHHHCCCCCCCHHHCCHHHCCCCCCEECCHHHHCCHHHHHHHHHHHHHCCCCCC
WDAPLKDLYAKRK
CCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8635745; 7542800 [H]