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The map label for this gene is ribF [H]

Identifier: 157960910

GI number: 157960910

Start: 1309724

End: 1310659

Strand: Direct

Name: ribF [H]

Synonym: Spea_1082

Alternate gene names: 157960910

Gene position: 1309724-1310659 (Clockwise)

Preceding gene: 157960909

Following gene: 157960911

Centisome position: 25.31

GC content: 43.06

Gene sequence:

>936_bases
ATGGAATTAATCCGCGGTATTCATAACATTTTGCCGAAACATCGAGGTTGCGTATTAAGCATAGGCAACTTTGATGGTGT
TCACCGTGGTCATGCGGAAGTGATTAATCGGTTAGTCAAGAAAGCAGAGCACCTAGGTTTGCCTGCCGCAGTGATGACCT
TTGAGCCACAACCGCGAGAGTTATTTCAGGGTGAAAGTGCACCAGCTCGTTTAAGTTTGTTGCGAGACAAGATAGTCTTG
CTCGATGAGCTAAAGATCGACCGCTTATTGTGTGTAAACTTTAATAATAAGTTTTCCAGTTATAGCGCTGAAGATTTTAT
TGAGCAGTTACTCGTTAAGGCTTTAGGTGTTAAGTACTTAGTTGTTGGTGACGATTTTTGCTTTGGTAAACAGCGCCAAG
GTAATTTTGATATGTTACGCAAAGCCGGTGAGAAATTCGGTTTTGCAGTGGTTAGCACTCAAAGTTTTATTTTGGGCGAT
AAGCGTGTCAGTTCAACGGAAATCCGCAAGTTACTTGCAAAAGGCAACCTAGAGCAAGCAAGACGATTATTAGGGCACCC
TTTCACCTTAAGTGGCAAGGTGGCTCATGGTGAGAAAATTGGTCGAACGATTGGTTTTCCTACTGCTAATATTGCTTTAA
AGCGTAAAGTTACACCTGTTCGAGGTGTATTTGCGGTCAAGCTATATTGGGATAACAGCGATATATATGAAGGCGTGGCT
AACGTTGGCTTTAGACCTACGGTAAACGGCCAAGTCTGTCAGTTAGAAGTCCACCTATTTGATTTTGACGGCGACCTTTA
TGGTAAAACCGTCGAAGTGGAGTTAGTGGCAAAGATTAGAGACGAGCAGCCATTTGGATCGTTAGATGCGCTGAAAAAAC
AAATTAAAGATGATGCCAATAGAGCGAAAGCCCTACTTGGTAGCGATGCAGGCTGA

Upstream 100 bases:

>100_bases
GAATTCGTCCTTGGAAGATGAAAGCTGCAGTTTGAGTGCTGATTATAAAGGCTAGCTGGTATATAATCCGCCCATTTACT
CTCATGTGTCGTGTAAGATA

Downstream 100 bases:

>100_bases
ACAGCTTAGCGAACAAAACGGTAAAGGATCAATGAGCGACTATAAATCTACTTTGAATTTGCCGGAAACAGAGTTTCCGA
TGCGTGGTAATTTGGCTAAT

Product: riboflavin biosynthesis protein RibF

Products: NA

Alternate protein names: Riboflavin kinase; Flavokinase; FMN adenylyltransferase; FAD pyrophosphorylase; FAD synthase [H]

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MELIRGIHNILPKHRGCVLSIGNFDGVHRGHAEVINRLVKKAEHLGLPAAVMTFEPQPRELFQGESAPARLSLLRDKIVL
LDELKIDRLLCVNFNNKFSSYSAEDFIEQLLVKALGVKYLVVGDDFCFGKQRQGNFDMLRKAGEKFGFAVVSTQSFILGD
KRVSSTEIRKLLAKGNLEQARRLLGHPFTLSGKVAHGEKIGRTIGFPTANIALKRKVTPVRGVFAVKLYWDNSDIYEGVA
NVGFRPTVNGQVCQLEVHLFDFDGDLYGKTVEVELVAKIRDEQPFGSLDALKKQIKDDANRAKALLGSDAG

Sequences:

>Translated_311_residues
MELIRGIHNILPKHRGCVLSIGNFDGVHRGHAEVINRLVKKAEHLGLPAAVMTFEPQPRELFQGESAPARLSLLRDKIVL
LDELKIDRLLCVNFNNKFSSYSAEDFIEQLLVKALGVKYLVVGDDFCFGKQRQGNFDMLRKAGEKFGFAVVSTQSFILGD
KRVSSTEIRKLLAKGNLEQARRLLGHPFTLSGKVAHGEKIGRTIGFPTANIALKRKVTPVRGVFAVKLYWDNSDIYEGVA
NVGFRPTVNGQVCQLEVHLFDFDGDLYGKTVEVELVAKIRDEQPFGSLDALKKQIKDDANRAKALLGSDAG
>Mature_311_residues
MELIRGIHNILPKHRGCVLSIGNFDGVHRGHAEVINRLVKKAEHLGLPAAVMTFEPQPRELFQGESAPARLSLLRDKIVL
LDELKIDRLLCVNFNNKFSSYSAEDFIEQLLVKALGVKYLVVGDDFCFGKQRQGNFDMLRKAGEKFGFAVVSTQSFILGD
KRVSSTEIRKLLAKGNLEQARRLLGHPFTLSGKVAHGEKIGRTIGFPTANIALKRKVTPVRGVFAVKLYWDNSDIYEGVA
NVGFRPTVNGQVCQLEVHLFDFDGDLYGKTVEVELVAKIRDEQPFGSLDALKKQIKDDANRAKALLGSDAG

Specific function: Unknown

COG id: COG0196

COG function: function code H; FAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribF family [H]

Homologues:

Organism=Escherichia coli, GI1786208, Length=308, Percent_Identity=52.9220779220779, Blast_Score=321, Evalue=3e-89,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004821
- InterPro:   IPR015864
- InterPro:   IPR015865
- InterPro:   IPR002606
- InterPro:   IPR014729 [H]

Pfam domain/function: PF06574 FAD_syn; PF01687 Flavokinase [H]

EC number: =2.7.1.26; =2.7.7.2 [H]

Molecular weight: Translated: 34515; Mature: 34515

Theoretical pI: Translated: 9.70; Mature: 9.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELIRGIHNILPKHRGCVLSIGNFDGVHRGHAEVINRLVKKAEHLGLPAAVMTFEPQPRE
CHHHHHHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCHH
LFQGESAPARLSLLRDKIVLLDELKIDRLLCVNFNNKFSSYSAEDFIEQLLVKALGVKYL
HHCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCEEE
VVGDDFCFGKQRQGNFDMLRKAGEKFGFAVVSTQSFILGDKRVSSTEIRKLLAKGNLEQA
EEECCCCCCCCCCCCHHHHHHHHHHHCEEEEECCEEEECCCCCCHHHHHHHHHCCCHHHH
RRLLGHPFTLSGKVAHGEKIGRTIGFPTANIALKRKVTPVRGVFAVKLYWDNSDIYEGVA
HHHHCCCCEECCCCCCCHHHHHCCCCCCCCEEEEECCCCCCCEEEEEEEECCCHHHHHHH
NVGFRPTVNGQVCQLEVHLFDFDGDLYGKTVEVELVAKIRDEQPFGSLDALKKQIKDDAN
HCCCCCCCCCEEEEEEEEEEECCCCCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHH
RAKALLGSDAG
HHHHHHCCCCC
>Mature Secondary Structure
MELIRGIHNILPKHRGCVLSIGNFDGVHRGHAEVINRLVKKAEHLGLPAAVMTFEPQPRE
CHHHHHHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCHH
LFQGESAPARLSLLRDKIVLLDELKIDRLLCVNFNNKFSSYSAEDFIEQLLVKALGVKYL
HHCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCEEE
VVGDDFCFGKQRQGNFDMLRKAGEKFGFAVVSTQSFILGDKRVSSTEIRKLLAKGNLEQA
EEECCCCCCCCCCCCHHHHHHHHHHHCEEEEECCEEEECCCCCCHHHHHHHHHCCCHHHH
RRLLGHPFTLSGKVAHGEKIGRTIGFPTANIALKRKVTPVRGVFAVKLYWDNSDIYEGVA
HHHHCCCCEECCCCCCCHHHHHCCCCCCCCEEEEECCCCCCCEEEEEEEECCCHHHHHHH
NVGFRPTVNGQVCQLEVHLFDFDGDLYGKTVEVELVAKIRDEQPFGSLDALKKQIKDDAN
HCCCCCCCCCEEEEEEEEEEECCCCCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHH
RAKALLGSDAG
HHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]