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The map label for this gene is alsT [H]

Identifier: 157960905

GI number: 157960905

Start: 1303980

End: 1305461

Strand: Direct

Name: alsT [H]

Synonym: Spea_1077

Alternate gene names: 157960905

Gene position: 1303980-1305461 (Clockwise)

Preceding gene: 157960899

Following gene: 157960906

Centisome position: 25.2

GC content: 46.15

Gene sequence:

>1482_bases
ATGATAGAAACAACGGTTAATTTTCTCAATGCGCTGCTTTGGGGAAAACTATTAGTATATGGTCTGGTTGGTGCTGGACT
GTACTTTACAATTCGACTGGCATTTATTCAGCTTACTCATTTTAAGCATTCTGGCGAAATCATGCTTAAGAGTCGTCAAG
GCGGCGGACGAAGCGGCCTTTCTTCTTTCCAGGTTTTCTGTACCAGTATGGCGGCTCGTGTTGGCGCTGGTAATATGGCC
GGTGTGGCGGTTGCGATTACAGCGGCAGGCCCAGGTGCAGTATTCTGGATGTGGCTTATTGCCATCCTAGGTATGGCAAC
GGCAATGGTTGAGTCAACATTGGCGCAGGTTTATAAAGTACGCGATCAAGACGGGCAATACCGCGGTGGCCCTTCCTATT
ACATGGAAAAGGGCCTTGGTCAGCGTTGGATGGGAATTTTATTCTCAATCTTCTTGATCATTGCTTTCGGCCTAGTGTTT
AACGCTGTTCAAGCCAATACCATTACTGGTGCAATGACTCAGGTCTTTAATTTAGATGCGACTTACGTCGGCATCGCAAT
TGTGATCCTCAGTGCCTTTGTGATTATGGGCGGCTTAAATAAAGTGGCTAAGGTATCGGAAATCGTCGTTCCGATTATGG
CTATTGCCTATATCTTGATCGCTTTTGTTGTTGTGGCAATGAACATCGAGAAGCTTCCTGATGTATTTATGCTGGTGATA
AAGAGTGCGTTCGGTCTGCAAGAAGCCGTTGCAGGTGGTGTGGCATATACTATCGCTCAAGCGATGCAAGCTGGTATCGC
TCGAGGTTTATTCTCTAACGAAGCGGGTATGGGTAGCGCGGCAAACGTCGCAGCGAGTGCATCGCCAAATCCAAATCATC
CAGCGTCGCAAGGCTTTGTACAGATGATGGGTGTTTTTGTCGATACCATAGTTATCTGTACAGCAACAGCGGCAATTATT
TTATTGGCGGGTGACTTAGGTTCAGACGGTGATGGCATTAAGCTAACCATCGAGGCATTGACACAACACGTTGGACCTTG
GGGTGGTGGATTTGTTGCATTAGCAATTTTGCTATTCTGCTTTACCTCTATCATTGCTAACTACTCTTATGCTGAAACCA
ACGTAATGTACTTGTCGGGTAACAGCAAGAAAGCACTGCCTATCTTTAGAGTGTGCGTTTTAGGTATGGTGATGTTCGGT
GCTGTAGCTAAGATTGGTCTAGTGTGGAACTTAGCCGATGTGTCTATGGGCCTAATGGCGACTGTGAACATTATTGCCTT
ATTACTGCTGTCTGGTTTAGCGATTAGAGTGATTAACGATTATCGTCAACAATTAAAAAGTGGCGAAACTCCAACCTTCG
ATGCAAGCAAGTTCCCTGAGCTATCTAATTTAGAAGGTGGGATCTGGACCAGCAAAACCGTTGAAGAAGAAGCCAAGGCT
CAGGCTAAGGCCGAAGCGAAAGCAGAAGCTGCTGGCGCTTAA

Upstream 100 bases:

>100_bases
AAATCTGCTGTTATTTAAATACTTAGGAAGGTTAATACAGGATAGACACCATCGATGGCTGGTTTAGCTCGATTGGGTTT
GTCATGGGGATTGAAATAAT

Downstream 100 bases:

>100_bases
TCTATAAGCGGTATCAGTACAATCTAAAAAACCACGCCGTTTAAGCGTGGTTTTTTTGTTTTTGTTCAGTATCATTGTCT
TAGAATATCTTTAAAGGACC

Product: amino acid carrier protein

Products: Na (I) [Cytoplasm]; L-alanine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 493; Mature: 493

Protein sequence:

>493_residues
MIETTVNFLNALLWGKLLVYGLVGAGLYFTIRLAFIQLTHFKHSGEIMLKSRQGGGRSGLSSFQVFCTSMAARVGAGNMA
GVAVAITAAGPGAVFWMWLIAILGMATAMVESTLAQVYKVRDQDGQYRGGPSYYMEKGLGQRWMGILFSIFLIIAFGLVF
NAVQANTITGAMTQVFNLDATYVGIAIVILSAFVIMGGLNKVAKVSEIVVPIMAIAYILIAFVVVAMNIEKLPDVFMLVI
KSAFGLQEAVAGGVAYTIAQAMQAGIARGLFSNEAGMGSAANVAASASPNPNHPASQGFVQMMGVFVDTIVICTATAAII
LLAGDLGSDGDGIKLTIEALTQHVGPWGGGFVALAILLFCFTSIIANYSYAETNVMYLSGNSKKALPIFRVCVLGMVMFG
AVAKIGLVWNLADVSMGLMATVNIIALLLLSGLAIRVINDYRQQLKSGETPTFDASKFPELSNLEGGIWTSKTVEEEAKA
QAKAEAKAEAAGA

Sequences:

>Translated_493_residues
MIETTVNFLNALLWGKLLVYGLVGAGLYFTIRLAFIQLTHFKHSGEIMLKSRQGGGRSGLSSFQVFCTSMAARVGAGNMA
GVAVAITAAGPGAVFWMWLIAILGMATAMVESTLAQVYKVRDQDGQYRGGPSYYMEKGLGQRWMGILFSIFLIIAFGLVF
NAVQANTITGAMTQVFNLDATYVGIAIVILSAFVIMGGLNKVAKVSEIVVPIMAIAYILIAFVVVAMNIEKLPDVFMLVI
KSAFGLQEAVAGGVAYTIAQAMQAGIARGLFSNEAGMGSAANVAASASPNPNHPASQGFVQMMGVFVDTIVICTATAAII
LLAGDLGSDGDGIKLTIEALTQHVGPWGGGFVALAILLFCFTSIIANYSYAETNVMYLSGNSKKALPIFRVCVLGMVMFG
AVAKIGLVWNLADVSMGLMATVNIIALLLLSGLAIRVINDYRQQLKSGETPTFDASKFPELSNLEGGIWTSKTVEEEAKA
QAKAEAKAEAAGA
>Mature_493_residues
MIETTVNFLNALLWGKLLVYGLVGAGLYFTIRLAFIQLTHFKHSGEIMLKSRQGGGRSGLSSFQVFCTSMAARVGAGNMA
GVAVAITAAGPGAVFWMWLIAILGMATAMVESTLAQVYKVRDQDGQYRGGPSYYMEKGLGQRWMGILFSIFLIIAFGLVF
NAVQANTITGAMTQVFNLDATYVGIAIVILSAFVIMGGLNKVAKVSEIVVPIMAIAYILIAFVVVAMNIEKLPDVFMLVI
KSAFGLQEAVAGGVAYTIAQAMQAGIARGLFSNEAGMGSAANVAASASPNPNHPASQGFVQMMGVFVDTIVICTATAAII
LLAGDLGSDGDGIKLTIEALTQHVGPWGGGFVALAILLFCFTSIIANYSYAETNVMYLSGNSKKALPIFRVCVLGMVMFG
AVAKIGLVWNLADVSMGLMATVNIIALLLLSGLAIRVINDYRQQLKSGETPTFDASKFPELSNLEGGIWTSKTVEEEAKA
QAKAEAKAEAAGA

Specific function: Unknown

COG id: COG1115

COG function: function code E; Na+/alanine symporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:alanine (SAF) symporter family [H]

Homologues:

Organism=Escherichia coli, GI1786188, Length=472, Percent_Identity=46.3983050847458, Blast_Score=421, Evalue=1e-119,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001463 [H]

Pfam domain/function: PF01235 Na_Ala_symp [H]

EC number: NA

Molecular weight: Translated: 51961; Mature: 51961

Theoretical pI: Translated: 7.93; Mature: 7.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIETTVNFLNALLWGKLLVYGLVGAGLYFTIRLAFIQLTHFKHSGEIMLKSRQGGGRSGL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCH
SSFQVFCTSMAARVGAGNMAGVAVAITAAGPGAVFWMWLIAILGMATAMVESTLAQVYKV
HHHHHHHHHHHHHHCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RDQDGQYRGGPSYYMEKGLGQRWMGILFSIFLIIAFGLVFNAVQANTITGAMTQVFNLDA
HCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
TYVGIAIVILSAFVIMGGLNKVAKVSEIVVPIMAIAYILIAFVVVAMNIEKLPDVFMLVI
HHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KSAFGLQEAVAGGVAYTIAQAMQAGIARGLFSNEAGMGSAANVAASASPNPNHPASQGFV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCCCCCCCCCCCCHHHHHH
QMMGVFVDTIVICTATAAIILLAGDLGSDGDGIKLTIEALTQHVGPWGGGFVALAILLFC
HHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCEEEHHHHHHHCCCCCCHHHHHHHHHHH
FTSIIANYSYAETNVMYLSGNSKKALPIFRVCVLGMVMFGAVAKIGLVWNLADVSMGLMA
HHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TVNIIALLLLSGLAIRVINDYRQQLKSGETPTFDASKFPELSNLEGGIWTSKTVEEEAKA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
QAKAEAKAEAAGA
HHHHHHHHHCCCC
>Mature Secondary Structure
MIETTVNFLNALLWGKLLVYGLVGAGLYFTIRLAFIQLTHFKHSGEIMLKSRQGGGRSGL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCH
SSFQVFCTSMAARVGAGNMAGVAVAITAAGPGAVFWMWLIAILGMATAMVESTLAQVYKV
HHHHHHHHHHHHHHCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RDQDGQYRGGPSYYMEKGLGQRWMGILFSIFLIIAFGLVFNAVQANTITGAMTQVFNLDA
HCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
TYVGIAIVILSAFVIMGGLNKVAKVSEIVVPIMAIAYILIAFVVVAMNIEKLPDVFMLVI
HHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KSAFGLQEAVAGGVAYTIAQAMQAGIARGLFSNEAGMGSAANVAASASPNPNHPASQGFV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCCCCCCCCCCCCHHHHHH
QMMGVFVDTIVICTATAAIILLAGDLGSDGDGIKLTIEALTQHVGPWGGGFVALAILLFC
HHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCEEEHHHHHHHCCCCCCHHHHHHHHHHH
FTSIIANYSYAETNVMYLSGNSKKALPIFRVCVLGMVMFGAVAKIGLVWNLADVSMGLMA
HHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TVNIIALLLLSGLAIRVINDYRQQLKSGETPTFDASKFPELSNLEGGIWTSKTVEEEAKA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
QAKAEAKAEAAGA
HHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Periplasm]; L-alanine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-alanine [Periplasm] = Na (I) [Cytoplasm] + L-alanine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969507; 9384377; 7710280 [H]