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The map label for this gene is lepA [H]

Identifier: 157960863

GI number: 157960863

Start: 1257261

End: 1259051

Strand: Direct

Name: lepA [H]

Synonym: Spea_1035

Alternate gene names: 157960863

Gene position: 1257261-1259051 (Clockwise)

Preceding gene: 157960862

Following gene: 157960864

Centisome position: 24.3

GC content: 43.66

Gene sequence:

>1791_bases
ATGAAGCATATTAGAAACTTTTCAATTATTGCCCATATTGACCACGGCAAATCAACACTATCAGATCGCCTTATTCAAGA
ATGCGGTGGCTTAACCGATCGTGAAATGGCTGCGCAAGTTCTTGACTCAATGGATATTGAGCGTGAGCGCGGTATTACCA
TCAAAGCCCAAAGTGTAACTTTGGATTACTTAGCTAATGATGGTGAAACCTACCAGCTAAACTTCATTGATACCCCTGGC
CACGTTGACTTCTCTTATGAAGTATCACGTTCGCTTGCAGCATGTGAAGGGGCATTATTGGTTGTTGACGCTGGTCAAGG
CGTTGAGGCACAAACCCTAGCAAACTGTTATACCGCACTTGAGATGGATATGGACGTTGTTCCTGTTCTGAACAAGATCG
ATCTGCCGCAGGCTGATCCAGACCGTGTAGCTGAAGAAATTGAAGACATCGTGGGCATTGAAGCTACTGATGCAGTTCGA
TGTTCAGCTAAAACGGGTATCGGTATTAAAGATGTTCTTGAAGTTATCGTCGAGCAAATTCCACCGCCAGAAGGCGATCC
AGAAGGTCCACTTCAAGCGCTGATTATCGACTCTTGGTTCGATAGCTACTTAGGCGTCGTGTCTCTAGTTCGTATCAAGA
ACGGCATTTTGAAAAAAGGCGACAAGTTTAAGGTTATGTCTACAGGGCAGAACTACAACGCTGATCGTGTTGGTATTTTC
ACGCCTAAGCAAACCGATACTACCGAGCTAAAAACCGGTGAAGTAGGCTTCGTTATTGCGGGTATTAAAGAGATTCACGG
TGCACCTGTAGGTGATACCTTGACTCACTCTAAGCACGGTGCTGAAACACCTTTAGCAGGTTTTAAAAAGGTTAAACCAC
AAGTTTATGCTGGTTTATTCCCAATCTCTACTGACGATTACGAAAACTTCCGTGACGCGCTGAACAAGCTAAGCTTGAAC
GATGCATCACTATTTTTCGAACCAGAAACTTCATCTGCACTAGGTTTTGGTTTCCGTATTGGCTTCTTGGGTCTGCTCCA
CATGGAGATTATTCAAGAGCGTCTAGAGCGTGAATACAACTTAGAGCTAATCACTACAGCGCCAACGGTAGTGTATGAAA
TCGTTCAGACTAATGGTGAGACGATCTACGTTGATAACCCATCTGATCTGCCAGCGGTTAACAATATCGCTGAGATGCGT
GAGCCGATTGTTGAAACCAATATTTTGGTTCCAAAAGAATACTTAGGTAACGTAATTACCCTATGTATCGAAAAGCGTGG
TGTACAAACCAATTTGGTTTACCACGGTAACCAAGTGGCGTTGACCTACGAATTACCAATGGCTGAAGTGGTCATGGATT
TCTTCGACCGTCTTAAATCAACCAGTCGCGGTTATGCTTCTCTTGAGTATAACTTTATCCGCTTTGAGCCTGCTGACATG
GTACGTTTAGATATCTTGATCAACGGTGACAGAGTCGATGCATTGGCCATGATTATCCACAAAGGATTGATCCGCTCTAA
AGGTCTAGCGCTTGTGAATAAGATGAAAGAGCTTATTCCTCGTCAAATGTTTGATATTGCGGTACAAGCGGCGGTTGGTA
GTCAGATTATTGCGCGTTCATCGATTAAAGCTATGCGTAAAGACGTAACTGCTAAATGTTACGGTGGTGACGTATCTCGT
AAGAAGAAACTACTGAACAAGCAGAAAGAAGGTAAGAAACGTATGAAGCAGGTTGGTAACGTTGAGGTGCCTCAAGAAGC
ATTCTTAGCGGTACTAAAGCTTAACGATTAA

Upstream 100 bases:

>100_bases
CTCAGTTATCAGACTATAAAAATTGGTATTAAAGCTGCGATCGGGTACAATTCTGCACTTTAAAATTTTTATCAACTTCG
AGTTAAGTCATCCCAGCATA

Downstream 100 bases:

>100_bases
GCTTACTCGTTAAGAATTGTTAATATATCGCTAGACTGAGCGCCGCAATTTGCGGCGCTTTCATTACTGGCGTATAACCA
TTAATGACAATGGCGCTATC

Product: GTP-binding protein LepA

Products: NA

Alternate protein names: EF-4; Ribosomal back-translocase LepA [H]

Number of amino acids: Translated: 596; Mature: 596

Protein sequence:

>596_residues
MKHIRNFSIIAHIDHGKSTLSDRLIQECGGLTDREMAAQVLDSMDIERERGITIKAQSVTLDYLANDGETYQLNFIDTPG
HVDFSYEVSRSLAACEGALLVVDAGQGVEAQTLANCYTALEMDMDVVPVLNKIDLPQADPDRVAEEIEDIVGIEATDAVR
CSAKTGIGIKDVLEVIVEQIPPPEGDPEGPLQALIIDSWFDSYLGVVSLVRIKNGILKKGDKFKVMSTGQNYNADRVGIF
TPKQTDTTELKTGEVGFVIAGIKEIHGAPVGDTLTHSKHGAETPLAGFKKVKPQVYAGLFPISTDDYENFRDALNKLSLN
DASLFFEPETSSALGFGFRIGFLGLLHMEIIQERLEREYNLELITTAPTVVYEIVQTNGETIYVDNPSDLPAVNNIAEMR
EPIVETNILVPKEYLGNVITLCIEKRGVQTNLVYHGNQVALTYELPMAEVVMDFFDRLKSTSRGYASLEYNFIRFEPADM
VRLDILINGDRVDALAMIIHKGLIRSKGLALVNKMKELIPRQMFDIAVQAAVGSQIIARSSIKAMRKDVTAKCYGGDVSR
KKKLLNKQKEGKKRMKQVGNVEVPQEAFLAVLKLND

Sequences:

>Translated_596_residues
MKHIRNFSIIAHIDHGKSTLSDRLIQECGGLTDREMAAQVLDSMDIERERGITIKAQSVTLDYLANDGETYQLNFIDTPG
HVDFSYEVSRSLAACEGALLVVDAGQGVEAQTLANCYTALEMDMDVVPVLNKIDLPQADPDRVAEEIEDIVGIEATDAVR
CSAKTGIGIKDVLEVIVEQIPPPEGDPEGPLQALIIDSWFDSYLGVVSLVRIKNGILKKGDKFKVMSTGQNYNADRVGIF
TPKQTDTTELKTGEVGFVIAGIKEIHGAPVGDTLTHSKHGAETPLAGFKKVKPQVYAGLFPISTDDYENFRDALNKLSLN
DASLFFEPETSSALGFGFRIGFLGLLHMEIIQERLEREYNLELITTAPTVVYEIVQTNGETIYVDNPSDLPAVNNIAEMR
EPIVETNILVPKEYLGNVITLCIEKRGVQTNLVYHGNQVALTYELPMAEVVMDFFDRLKSTSRGYASLEYNFIRFEPADM
VRLDILINGDRVDALAMIIHKGLIRSKGLALVNKMKELIPRQMFDIAVQAAVGSQIIARSSIKAMRKDVTAKCYGGDVSR
KKKLLNKQKEGKKRMKQVGNVEVPQEAFLAVLKLND
>Mature_596_residues
MKHIRNFSIIAHIDHGKSTLSDRLIQECGGLTDREMAAQVLDSMDIERERGITIKAQSVTLDYLANDGETYQLNFIDTPG
HVDFSYEVSRSLAACEGALLVVDAGQGVEAQTLANCYTALEMDMDVVPVLNKIDLPQADPDRVAEEIEDIVGIEATDAVR
CSAKTGIGIKDVLEVIVEQIPPPEGDPEGPLQALIIDSWFDSYLGVVSLVRIKNGILKKGDKFKVMSTGQNYNADRVGIF
TPKQTDTTELKTGEVGFVIAGIKEIHGAPVGDTLTHSKHGAETPLAGFKKVKPQVYAGLFPISTDDYENFRDALNKLSLN
DASLFFEPETSSALGFGFRIGFLGLLHMEIIQERLEREYNLELITTAPTVVYEIVQTNGETIYVDNPSDLPAVNNIAEMR
EPIVETNILVPKEYLGNVITLCIEKRGVQTNLVYHGNQVALTYELPMAEVVMDFFDRLKSTSRGYASLEYNFIRFEPADM
VRLDILINGDRVDALAMIIHKGLIRSKGLALVNKMKELIPRQMFDIAVQAAVGSQIIARSSIKAMRKDVTAKCYGGDVSR
KKKLLNKQKEGKKRMKQVGNVEVPQEAFLAVLKLND

Specific function: Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back- transloc

COG id: COG0481

COG function: function code M; Membrane GTPase LepA

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. LepA subfamily [H]

Homologues:

Organism=Homo sapiens, GI157426893, Length=602, Percent_Identity=47.8405315614618, Blast_Score=608, Evalue=1e-174,
Organism=Homo sapiens, GI94966754, Length=134, Percent_Identity=45.5223880597015, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI25306283, Length=149, Percent_Identity=42.9530201342282, Blast_Score=102, Evalue=7e-22,
Organism=Homo sapiens, GI19923640, Length=149, Percent_Identity=42.9530201342282, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI25306287, Length=134, Percent_Identity=45.5223880597015, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI4503483, Length=144, Percent_Identity=40.2777777777778, Blast_Score=100, Evalue=5e-21,
Organism=Homo sapiens, GI18390331, Length=150, Percent_Identity=35.3333333333333, Blast_Score=96, Evalue=1e-19,
Organism=Homo sapiens, GI310132016, Length=111, Percent_Identity=43.2432432432432, Blast_Score=93, Evalue=7e-19,
Organism=Homo sapiens, GI310110807, Length=111, Percent_Identity=43.2432432432432, Blast_Score=93, Evalue=7e-19,
Organism=Homo sapiens, GI310123363, Length=111, Percent_Identity=43.2432432432432, Blast_Score=93, Evalue=7e-19,
Organism=Homo sapiens, GI53729339, Length=245, Percent_Identity=29.7959183673469, Blast_Score=84, Evalue=5e-16,
Organism=Homo sapiens, GI53729337, Length=245, Percent_Identity=29.7959183673469, Blast_Score=84, Evalue=5e-16,
Organism=Homo sapiens, GI217272894, Length=168, Percent_Identity=33.3333333333333, Blast_Score=83, Evalue=7e-16,
Organism=Homo sapiens, GI217272892, Length=168, Percent_Identity=33.3333333333333, Blast_Score=83, Evalue=7e-16,
Organism=Homo sapiens, GI34147630, Length=255, Percent_Identity=28.6274509803922, Blast_Score=70, Evalue=8e-12,
Organism=Escherichia coli, GI1788922, Length=594, Percent_Identity=80.8080808080808, Blast_Score=972, Evalue=0.0,
Organism=Escherichia coli, GI48994988, Length=508, Percent_Identity=28.740157480315, Blast_Score=175, Evalue=9e-45,
Organism=Escherichia coli, GI1789738, Length=153, Percent_Identity=35.2941176470588, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1789559, Length=229, Percent_Identity=31.8777292576419, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI1790835, Length=156, Percent_Identity=32.0512820512821, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1789737, Length=336, Percent_Identity=27.3809523809524, Blast_Score=66, Evalue=6e-12,
Organism=Escherichia coli, GI1790412, Length=336, Percent_Identity=27.3809523809524, Blast_Score=66, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17557151, Length=611, Percent_Identity=41.4075286415712, Blast_Score=489, Evalue=1e-138,
Organism=Caenorhabditis elegans, GI17556745, Length=464, Percent_Identity=25.2155172413793, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17533571, Length=146, Percent_Identity=34.9315068493151, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI71988819, Length=135, Percent_Identity=37.7777777777778, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71988811, Length=135, Percent_Identity=37.7777777777778, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17506493, Length=170, Percent_Identity=34.7058823529412, Blast_Score=90, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17552882, Length=145, Percent_Identity=35.1724137931034, Blast_Score=83, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI71994658, Length=257, Percent_Identity=26.0700389105058, Blast_Score=73, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI32566303, Length=244, Percent_Identity=27.8688524590164, Blast_Score=69, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI25141371, Length=323, Percent_Identity=25.3869969040248, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323320, Length=602, Percent_Identity=45.514950166113, Blast_Score=540, Evalue=1e-154,
Organism=Saccharomyces cerevisiae, GI6323098, Length=158, Percent_Identity=37.3417721518987, Blast_Score=106, Evalue=9e-24,
Organism=Saccharomyces cerevisiae, GI6324707, Length=144, Percent_Identity=41.6666666666667, Blast_Score=106, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6320593, Length=144, Percent_Identity=41.6666666666667, Blast_Score=106, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6322359, Length=115, Percent_Identity=38.2608695652174, Blast_Score=93, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6324166, Length=145, Percent_Identity=38.6206896551724, Blast_Score=81, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6325337, Length=273, Percent_Identity=25.6410256410256, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6319594, Length=273, Percent_Identity=25.6410256410256, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6324761, Length=248, Percent_Identity=27.8225806451613, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI78706572, Length=602, Percent_Identity=44.3521594684385, Blast_Score=544, Evalue=1e-155,
Organism=Drosophila melanogaster, GI28574573, Length=197, Percent_Identity=39.0862944162437, Blast_Score=102, Evalue=7e-22,
Organism=Drosophila melanogaster, GI24582462, Length=153, Percent_Identity=37.9084967320261, Blast_Score=98, Evalue=2e-20,
Organism=Drosophila melanogaster, GI221458488, Length=149, Percent_Identity=38.9261744966443, Blast_Score=93, Evalue=6e-19,
Organism=Drosophila melanogaster, GI24585709, Length=149, Percent_Identity=35.5704697986577, Blast_Score=92, Evalue=9e-19,
Organism=Drosophila melanogaster, GI24585711, Length=149, Percent_Identity=35.5704697986577, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24585713, Length=149, Percent_Identity=35.5704697986577, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI21357743, Length=133, Percent_Identity=36.0902255639098, Blast_Score=83, Evalue=5e-16,
Organism=Drosophila melanogaster, GI28572034, Length=226, Percent_Identity=29.2035398230088, Blast_Score=77, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR006297
- InterPro:   IPR013842
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000640
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF06421 LepA_C [H]

EC number: NA

Molecular weight: Translated: 65870; Mature: 65870

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHIRNFSIIAHIDHGKSTLSDRLIQECGGLTDREMAAQVLDSMDIERERGITIKAQSVT
CCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHCCCEEEEEEEE
LDYLANDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVVDAGQGVEAQTLANCYTAL
EEEEECCCCEEEEEEECCCCCEEEEHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHH
EMDMDVVPVLNKIDLPQADPDRVAEEIEDIVGIEATDAVRCSAKTGIGIKDVLEVIVEQI
HCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCEEECCCCCCCHHHHHHHHHHHC
PPPEGDPEGPLQALIIDSWFDSYLGVVSLVRIKNGILKKGDKFKVMSTGQNYNADRVGIF
CCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEE
TPKQTDTTELKTGEVGFVIAGIKEIHGAPVGDTLTHSKHGAETPLAGFKKVKPQVYAGLF
CCCCCCCCCCCCCCCEEEEECHHHHCCCCCCCCCCCCCCCCCCCCHHHHHCCCHHHCCEE
PISTDDYENFRDALNKLSLNDASLFFEPETSSALGFGFRIGFLGLLHMEIIQERLEREYN
CCCCCCHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
LELITTAPTVVYEIVQTNGETIYVDNPSDLPAVNNIAEMREPIVETNILVPKEYLGNVIT
EEEEECCHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
LCIEKRGVQTNLVYHGNQVALTYELPMAEVVMDFFDRLKSTSRGYASLEYNFIRFEPADM
HHHHHCCCEEEEEEECCEEEEEEECCHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCE
VRLDILINGDRVDALAMIIHKGLIRSKGLALVNKMKELIPRQMFDIAVQAAVGSQIIARS
EEEEEEECCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SIKAMRKDVTAKCYGGDVSRKKKLLNKQKEGKKRMKQVGNVEVPQEAFLAVLKLND
HHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHEEEEEEECC
>Mature Secondary Structure
MKHIRNFSIIAHIDHGKSTLSDRLIQECGGLTDREMAAQVLDSMDIERERGITIKAQSVT
CCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHCCCEEEEEEEE
LDYLANDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVVDAGQGVEAQTLANCYTAL
EEEEECCCCEEEEEEECCCCCEEEEHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHH
EMDMDVVPVLNKIDLPQADPDRVAEEIEDIVGIEATDAVRCSAKTGIGIKDVLEVIVEQI
HCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCEEECCCCCCCHHHHHHHHHHHC
PPPEGDPEGPLQALIIDSWFDSYLGVVSLVRIKNGILKKGDKFKVMSTGQNYNADRVGIF
CCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEE
TPKQTDTTELKTGEVGFVIAGIKEIHGAPVGDTLTHSKHGAETPLAGFKKVKPQVYAGLF
CCCCCCCCCCCCCCCEEEEECHHHHCCCCCCCCCCCCCCCCCCCCHHHHHCCCHHHCCEE
PISTDDYENFRDALNKLSLNDASLFFEPETSSALGFGFRIGFLGLLHMEIIQERLEREYN
CCCCCCHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
LELITTAPTVVYEIVQTNGETIYVDNPSDLPAVNNIAEMREPIVETNILVPKEYLGNVIT
EEEEECCHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
LCIEKRGVQTNLVYHGNQVALTYELPMAEVVMDFFDRLKSTSRGYASLEYNFIRFEPADM
HHHHHCCCEEEEEEECCEEEEEEECCHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCE
VRLDILINGDRVDALAMIIHKGLIRSKGLALVNKMKELIPRQMFDIAVQAAVGSQIIARS
EEEEEEECCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SIKAMRKDVTAKCYGGDVSRKKKLLNKQKEGKKRMKQVGNVEVPQEAFLAVLKLND
HHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA