Click here to switch to the map view.

The map label for this gene is rpoE [H]

Identifier: 157960859

GI number: 157960859

Start: 1254502

End: 1255080

Strand: Direct

Name: rpoE [H]

Synonym: Spea_1031

Alternate gene names: 157960859

Gene position: 1254502-1255080 (Clockwise)

Preceding gene: 157960857

Following gene: 157960860

Centisome position: 24.24

GC content: 45.6

Gene sequence:

>579_bases
ATGAGTGGACAAATAAGTGATCAACAGTTAGTTGAGCGCGTACAGCGTGGAGACAAAAACGCTTTTAACCTTTTGGTACA
GAAATATCAAAACAAGGTCGCGAATCTGATATCTCGTTACGTACGAAATCAGGCCGACGTTGCCGATGTTTCGCAGGAAG
CTTTTATTAAAGCTTACCGAGCACTACCAAACTTTCGTGGTGAAAGTGCGTTTTATACTTGGTTGTACCGCATTGCAGTG
AATACTGCTAAGAATCATCTGGTCGCTCAGGGACGTCGTGCCCCAGCGAATGATGTCGATGCTGAAGAAGCCGAATACTA
TGATGGCAGTGACGCGCTAAAGGAGTTTGCCTCCCCGGAGCGTCTGGTTTTGTCAGAAGAGATTCAAAAGGTTGTCTTCG
ACACTTTAGACACTTTGCCAGAAGAATTGAAGATGGCTATCTCACTTCGAGAGCTCGATGGTATGAGTTATGAAGAGATT
GCCAATGTCATGGATTGCCCTGTAGGCACTGTAAGATCACGGATCTTCAGAGCGCGAGAGGCAATCGATAAAAAGCTTAA
GCCGTTGCTAGAACGTTAG

Upstream 100 bases:

>100_bases
AAATTTTTTTAGAACTTTTTTGAAACTCGCTAGTCTACATAAGTGAATATGATTCGAGCGGCTGAGAAATCAGTATTAGA
TTTAGGAGTAGTCGGCTCGG

Downstream 100 bases:

>100_bases
CATCTTAAATAGATAGGTGAATAATGGATAAGTTAGGTCAGGAATGGGTATCTGCTGCTGTCGATGGTGAAATTGATGAG
CACACGTTAGCTGAATTAGT

Product: RNA polymerase sigma factor RpoE

Products: NA

Alternate protein names: Sigma-24 [H]

Number of amino acids: Translated: 192; Mature: 191

Protein sequence:

>192_residues
MSGQISDQQLVERVQRGDKNAFNLLVQKYQNKVANLISRYVRNQADVADVSQEAFIKAYRALPNFRGESAFYTWLYRIAV
NTAKNHLVAQGRRAPANDVDAEEAEYYDGSDALKEFASPERLVLSEEIQKVVFDTLDTLPEELKMAISLRELDGMSYEEI
ANVMDCPVGTVRSRIFRAREAIDKKLKPLLER

Sequences:

>Translated_192_residues
MSGQISDQQLVERVQRGDKNAFNLLVQKYQNKVANLISRYVRNQADVADVSQEAFIKAYRALPNFRGESAFYTWLYRIAV
NTAKNHLVAQGRRAPANDVDAEEAEYYDGSDALKEFASPERLVLSEEIQKVVFDTLDTLPEELKMAISLRELDGMSYEEI
ANVMDCPVGTVRSRIFRAREAIDKKLKPLLER
>Mature_191_residues
SGQISDQQLVERVQRGDKNAFNLLVQKYQNKVANLISRYVRNQADVADVSQEAFIKAYRALPNFRGESAFYTWLYRIAVN
TAKNHLVAQGRRAPANDVDAEEAEYYDGSDALKEFASPERLVLSEEIQKVVFDTLDTLPEELKMAISLRELDGMSYEEIA
NVMDCPVGTVRSRIFRAREAIDKKLKPLLER

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is involved in heat shock and oxidative stress response; it is believed to control protein processing

COG id: COG1595

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788926, Length=192, Percent_Identity=70.8333333333333, Blast_Score=284, Evalue=2e-78,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR014286
- InterPro:   IPR013325
- InterPro:   IPR013324 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 21907; Mature: 21776

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGQISDQQLVERVQRGDKNAFNLLVQKYQNKVANLISRYVRNQADVADVSQEAFIKAYR
CCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHH
ALPNFRGESAFYTWLYRIAVNTAKNHLVAQGRRAPANDVDAEEAEYYDGSDALKEFASPE
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCHHHHHHHCCCH
RLVLSEEIQKVVFDTLDTLPEELKMAISLRELDGMSYEEIANVMDCPVGTVRSRIFRARE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHH
AIDKKLKPLLER
HHHHHHHHHHCC
>Mature Secondary Structure 
SGQISDQQLVERVQRGDKNAFNLLVQKYQNKVANLISRYVRNQADVADVSQEAFIKAYR
CCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHH
ALPNFRGESAFYTWLYRIAVNTAKNHLVAQGRRAPANDVDAEEAEYYDGSDALKEFASPE
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCHHHHHHHCCCH
RLVLSEEIQKVVFDTLDTLPEELKMAISLRELDGMSYEEIANVMDCPVGTVRSRIFRARE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHH
AIDKKLKPLLER
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]