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The map label for this gene is 157960744

Identifier: 157960744

GI number: 157960744

Start: 1120318

End: 1121802

Strand: Reverse

Name: 157960744

Synonym: Spea_0916

Alternate gene names: NA

Gene position: 1121802-1120318 (Counterclockwise)

Preceding gene: 157960746

Following gene: 157960743

Centisome position: 21.68

GC content: 49.16

Gene sequence:

>1485_bases
ATGAACGCCAAAAGCAACCCGCACTATCAAACTTTAGTTAATCACTTTCAAAGTATTAGTCACTTCGAACACCTGAGCGC
ATTGGGTGATTGGGATCAGGCGACTATGATGCCAATGGGAGGCGGTGAAGCGCGTGGCGCCGCAATGGCAGAACTTGCAA
AACACATCCACTCATTGAAGACAGCTCCAATGTTGGCGCAGACAATTAGTGAAGCTGATGAGTTACCGCTGACAGAGCAA
CAAAAAGCCAATCTTACAGAGATGCGCTATCAGTACTTGCAAGCAAACGTGGTCCCTAGTGAATTGGTTGTAGCCAAAAC
GAAGGCAGCCTATCACTGCGAACATGCATGGCGTGAGCAACGTAGCAATAACGACTGGCAAGGCTTTAAGCCCAATCTTG
AAAACCTGATGAGCCTAGCCAAAGAGGAAGCCAATATTCGCGCCGAGGCGCAAAACGTTAGCCCTTATGATGCCCTGCTA
GATAAGTTCGAACCAGGCATGACTACGGCGAGTCTGGAGCAGATTTTTGGCAACTTAAAGAGTTGGTTACCCCAGTTAAT
CCAGCAGGTACAAGATAAGCAAAAGTCAGAAGCAAGTTTCACCATTCCAACGGCCAGCGCAGAAAGCCAGGCTAGGCTGG
GTCGTGAGATCATGAATATCTTGGACTTCGATTTTAACCACGGCCGACTCGATATCAGTAGCCACCCGTTTTGTGGTGGT
GTTGCCAGTGACGTACGTTTGACGACCCGTTACGATGAAGGCGATTTTACCAGTGGCTTAATGGGGATTGTCCATGAAAC
TGGCCACGCTAAATATGAGCAAGGCTTACCCTTAGCTTGGCGCGGTCAACCAGCGGGCCTTGCACGTTCCATGGCTCTGC
ACGAGAGTCAAAGTCTGTTCTGTGAGATGCAAGTTGGCCGTGGTCCCGCTTTTCTCAAGCTAATAGAGCCCTACCTAAGC
TCTCTACTGGGTAGCCAGTTAACTCACGATCAATTAACCAATATCTACACCCGAGTCACTCCTGGCTTGATCCGTGTCGA
TGCCGACGAGGTCACCTACCCTTGCCATATTCTACTGCGCTTCGAGGCAGAGAAAGCCTTAGTTAGCGGCGAGCTTGCCG
TGGCCGATCTTCCCGAATTCTGGTCATCGCAGATGCAGTCACTGTTAGGCATTAATACCGATGGCGATTATAAGAATGGC
TGCATGCAAGATATCCATTGGGCGGTGGGTGAGTTGGGTTACTTCCCAAGTTATACCTTAGGGGCCATGTATGCAGCGCA
ATTTAGAAATGCGATGGAATGCGAACTCGGCTGCATGGAAAGCCTCATAAACTCTGGGAAGCTTTCCAGTGTCATGGGCT
GGTTAAACGATAAAATTTGGTCTCAAGGTAGCCTACTGAGCATCGATGAGTTGATGCGTCAATCAACCGGTGAGACGCTC
AACCCTGAACACTTTAAAAAGCATCTTATCGGGCGCTATCTTTAA

Upstream 100 bases:

>100_bases
ACTGTGTTTGAGTCACCGCTTATAACCTCGGGCAAATTAGCATTTTAAGCCGGTTTATGTGCTAGCATAACGGCAAATAA
CCGGATTAAAATCAGACAAC

Downstream 100 bases:

>100_bases
GCACTAGATTTAAGCACTAGATTTAAGCACTAGATTTAAGTACTCGTAAAAAATATTACCACCTAAGTAGGACACTATAT
GAAGATCCGCCCTATTCAAG

Product: carboxypeptidase Taq

Products: NA

Alternate protein names: Carboxypeptidase Taq [H]

Number of amino acids: Translated: 494; Mature: 494

Protein sequence:

>494_residues
MNAKSNPHYQTLVNHFQSISHFEHLSALGDWDQATMMPMGGGEARGAAMAELAKHIHSLKTAPMLAQTISEADELPLTEQ
QKANLTEMRYQYLQANVVPSELVVAKTKAAYHCEHAWREQRSNNDWQGFKPNLENLMSLAKEEANIRAEAQNVSPYDALL
DKFEPGMTTASLEQIFGNLKSWLPQLIQQVQDKQKSEASFTIPTASAESQARLGREIMNILDFDFNHGRLDISSHPFCGG
VASDVRLTTRYDEGDFTSGLMGIVHETGHAKYEQGLPLAWRGQPAGLARSMALHESQSLFCEMQVGRGPAFLKLIEPYLS
SLLGSQLTHDQLTNIYTRVTPGLIRVDADEVTYPCHILLRFEAEKALVSGELAVADLPEFWSSQMQSLLGINTDGDYKNG
CMQDIHWAVGELGYFPSYTLGAMYAAQFRNAMECELGCMESLINSGKLSSVMGWLNDKIWSQGSLLSIDELMRQSTGETL
NPEHFKKHLIGRYL

Sequences:

>Translated_494_residues
MNAKSNPHYQTLVNHFQSISHFEHLSALGDWDQATMMPMGGGEARGAAMAELAKHIHSLKTAPMLAQTISEADELPLTEQ
QKANLTEMRYQYLQANVVPSELVVAKTKAAYHCEHAWREQRSNNDWQGFKPNLENLMSLAKEEANIRAEAQNVSPYDALL
DKFEPGMTTASLEQIFGNLKSWLPQLIQQVQDKQKSEASFTIPTASAESQARLGREIMNILDFDFNHGRLDISSHPFCGG
VASDVRLTTRYDEGDFTSGLMGIVHETGHAKYEQGLPLAWRGQPAGLARSMALHESQSLFCEMQVGRGPAFLKLIEPYLS
SLLGSQLTHDQLTNIYTRVTPGLIRVDADEVTYPCHILLRFEAEKALVSGELAVADLPEFWSSQMQSLLGINTDGDYKNG
CMQDIHWAVGELGYFPSYTLGAMYAAQFRNAMECELGCMESLINSGKLSSVMGWLNDKIWSQGSLLSIDELMRQSTGETL
NPEHFKKHLIGRYL
>Mature_494_residues
MNAKSNPHYQTLVNHFQSISHFEHLSALGDWDQATMMPMGGGEARGAAMAELAKHIHSLKTAPMLAQTISEADELPLTEQ
QKANLTEMRYQYLQANVVPSELVVAKTKAAYHCEHAWREQRSNNDWQGFKPNLENLMSLAKEEANIRAEAQNVSPYDALL
DKFEPGMTTASLEQIFGNLKSWLPQLIQQVQDKQKSEASFTIPTASAESQARLGREIMNILDFDFNHGRLDISSHPFCGG
VASDVRLTTRYDEGDFTSGLMGIVHETGHAKYEQGLPLAWRGQPAGLARSMALHESQSLFCEMQVGRGPAFLKLIEPYLS
SLLGSQLTHDQLTNIYTRVTPGLIRVDADEVTYPCHILLRFEAEKALVSGELAVADLPEFWSSQMQSLLGINTDGDYKNG
CMQDIHWAVGELGYFPSYTLGAMYAAQFRNAMECELGCMESLINSGKLSSVMGWLNDKIWSQGSLLSIDELMRQSTGETL
NPEHFKKHLIGRYL

Specific function: Releases amino acids sequentially from the C-terminus with a broad substrate specificity (except for proline) [H]

COG id: COG2317

COG function: function code E; Zn-dependent carboxypeptidase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M32 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001333 [H]

Pfam domain/function: PF02074 Peptidase_M32 [H]

EC number: =3.4.17.19 [H]

Molecular weight: Translated: 55096; Mature: 55096

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAKSNPHYQTLVNHFQSISHFEHLSALGDWDQATMMPMGGGEARGAAMAELAKHIHSLK
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHH
TAPMLAQTISEADELPLTEQQKANLTEMRYQYLQANVVPSELVVAKTKAAYHCEHAWREQ
HHHHHHHHHHHHHCCCCCCHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
RSNNDWQGFKPNLENLMSLAKEEANIRAEAQNVSPYDALLDKFEPGMTTASLEQIFGNLK
CCCCCCCCCCCCHHHHHHHHHHHHCCHHHCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHH
SWLPQLIQQVQDKQKSEASFTIPTASAESQARLGREIMNILDFDFNHGRLDISSHPFCGG
HHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCC
VASDVRLTTRYDEGDFTSGLMGIVHETGHAKYEQGLPLAWRGQPAGLARSMALHESQSLF
CCCCCEEEEECCCCCHHHHHHHHHHHCCCCHHHCCCCEEECCCCHHHHHHHHHHHCHHEE
CEMQVGRGPAFLKLIEPYLSSLLGSQLTHDQLTNIYTRVTPGLIRVDADEVTYPCHILLR
EEEECCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEECCCCCCCCEEEEEE
FEAEKALVSGELAVADLPEFWSSQMQSLLGINTDGDYKNGCMQDIHWAVGELGYFPSYTL
ECHHHHHHCCCCHHHHCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHH
GAMYAAQFRNAMECELGCMESLINSGKLSSVMGWLNDKIWSQGSLLSIDELMRQSTGETL
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEECHHHHHHHHCCCCC
NPEHFKKHLIGRYL
CHHHHHHHHHHCCC
>Mature Secondary Structure
MNAKSNPHYQTLVNHFQSISHFEHLSALGDWDQATMMPMGGGEARGAAMAELAKHIHSLK
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHH
TAPMLAQTISEADELPLTEQQKANLTEMRYQYLQANVVPSELVVAKTKAAYHCEHAWREQ
HHHHHHHHHHHHHCCCCCCHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
RSNNDWQGFKPNLENLMSLAKEEANIRAEAQNVSPYDALLDKFEPGMTTASLEQIFGNLK
CCCCCCCCCCCCHHHHHHHHHHHHCCHHHCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHH
SWLPQLIQQVQDKQKSEASFTIPTASAESQARLGREIMNILDFDFNHGRLDISSHPFCGG
HHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCC
VASDVRLTTRYDEGDFTSGLMGIVHETGHAKYEQGLPLAWRGQPAGLARSMALHESQSLF
CCCCCEEEEECCCCCHHHHHHHHHHHCCCCHHHCCCCEEECCCCHHHHHHHHHHHCHHEE
CEMQVGRGPAFLKLIEPYLSSLLGSQLTHDQLTNIYTRVTPGLIRVDADEVTYPCHILLR
EEEECCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEECCCCCCCCEEEEEE
FEAEKALVSGELAVADLPEFWSSQMQSLLGINTDGDYKNGCMQDIHWAVGELGYFPSYTL
ECHHHHHHCCCCHHHHCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHH
GAMYAAQFRNAMECELGCMESLINSGKLSSVMGWLNDKIWSQGSLLSIDELMRQSTGETL
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEECHHHHHHHHCCCCC
NPEHFKKHLIGRYL
CHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7765282; 1369078 [H]