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The map label for this gene is yafC [H]

Identifier: 157960732

GI number: 157960732

Start: 1105164

End: 1106072

Strand: Direct

Name: yafC [H]

Synonym: Spea_0904

Alternate gene names: 157960732

Gene position: 1105164-1106072 (Clockwise)

Preceding gene: 157960731

Following gene: 157960735

Centisome position: 21.36

GC content: 48.29

Gene sequence:

>909_bases
ATGGATCAGATAGGTGCGATGAAAGCGTTTGTGCGCGTGGTGCAAACTGGCAGCTTTTCTGCAGCGGCTCGTGAGCAAAG
TAGTACTCAGGCGACAATAAGCAAGAAAGTCGCTGCGCTTGAGAGTCTATTAGGTGCCAAGCTACTGACTCGTAGTAGTC
GTGAGCTATCGCTGACCGAAGTTGGCGCTGTTTATTTTGAAAATTGCGTGGCGATTATCGCCGAGCTCGATGAAGCGCAG
GCTCAGGTGCGCTCGCAGCAAGCATCGCCAAAAGGAATTCTACGCGTTGCTGCTCCTGTGGTTTTTGGTTGCCAGTTTAT
CGCGCCGATTTTGGGTGAGTTCTTGATCCGCTACCCTGAGATTAAAGTGGATTTGATGCTAAGCGATAAGCATGTAGATC
TGGTGGCTGAAGGGGTGGATCTTGCTATTCGTGCTAAGCAACTTGAGGATTCCTCCTTGGTTGCGAGGCATCTATTTGAT
AATCCACTGATCACGGTCGCATCACCTGAATACCTAAAACGTTTTACTCAACCCAAAGAACCTCAGGAACTGAGAGCCCA
TAACTGTATCGTCTATTCGATGTTAAAGTCAGTCAATATTTGGCACTTTGAACAGGCTAAGGGAGGCGGGGATAAAATTT
CAGTGCCTGTGGGCGGAAATTGCCGTTGTGATAACGGCGAGGCGATATTGCAGCTAGCCCTCGATGGTGTCGGCATCGCC
CAGTTGCCCACTTGGATGGTGGATAAGCATATTAACCAAGGTAAGCTGCAAAGGATGATGCCGAACTATCTATCTCACCC
TTTGCCCTTCAATGCTATCTATCCCCAAAGCCGCTATGTGCCGTTAAAGGTACGTTGTTTTATTGACTACCTAAAGCAAT
GTTTAGCGGAAGAGCCCTGCTACTATTAA

Upstream 100 bases:

>100_bases
GCTGCTAAAAAGCCAAGACGAACATAATAATTGTGATCAACGAGTGTGATGGTGTTCTGGGCCTAATTGCGTTAACTGGC
ATGGATAACGGAGAGATTAG

Downstream 100 bases:

>100_bases
GAGTAGGTACCACGGATCGGGTAGTTATTTTCTGGATCACGGATAAAAGATAAGCCCGATAGCAAAGTCGACAGTTCTGG
GCGCGCGAAGGGGCCATTAG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 302; Mature: 302

Protein sequence:

>302_residues
MDQIGAMKAFVRVVQTGSFSAAAREQSSTQATISKKVAALESLLGAKLLTRSSRELSLTEVGAVYFENCVAIIAELDEAQ
AQVRSQQASPKGILRVAAPVVFGCQFIAPILGEFLIRYPEIKVDLMLSDKHVDLVAEGVDLAIRAKQLEDSSLVARHLFD
NPLITVASPEYLKRFTQPKEPQELRAHNCIVYSMLKSVNIWHFEQAKGGGDKISVPVGGNCRCDNGEAILQLALDGVGIA
QLPTWMVDKHINQGKLQRMMPNYLSHPLPFNAIYPQSRYVPLKVRCFIDYLKQCLAEEPCYY

Sequences:

>Translated_302_residues
MDQIGAMKAFVRVVQTGSFSAAAREQSSTQATISKKVAALESLLGAKLLTRSSRELSLTEVGAVYFENCVAIIAELDEAQ
AQVRSQQASPKGILRVAAPVVFGCQFIAPILGEFLIRYPEIKVDLMLSDKHVDLVAEGVDLAIRAKQLEDSSLVARHLFD
NPLITVASPEYLKRFTQPKEPQELRAHNCIVYSMLKSVNIWHFEQAKGGGDKISVPVGGNCRCDNGEAILQLALDGVGIA
QLPTWMVDKHINQGKLQRMMPNYLSHPLPFNAIYPQSRYVPLKVRCFIDYLKQCLAEEPCYY
>Mature_302_residues
MDQIGAMKAFVRVVQTGSFSAAAREQSSTQATISKKVAALESLLGAKLLTRSSRELSLTEVGAVYFENCVAIIAELDEAQ
AQVRSQQASPKGILRVAAPVVFGCQFIAPILGEFLIRYPEIKVDLMLSDKHVDLVAEGVDLAIRAKQLEDSSLVARHLFD
NPLITVASPEYLKRFTQPKEPQELRAHNCIVYSMLKSVNIWHFEQAKGGGDKISVPVGGNCRCDNGEAILQLALDGVGIA
QLPTWMVDKHINQGKLQRMMPNYLSHPLPFNAIYPQSRYVPLKVRCFIDYLKQCLAEEPCYY

Specific function: Unknown

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=293, Percent_Identity=36.8600682593857, Blast_Score=199, Evalue=1e-52,
Organism=Escherichia coli, GI1789639, Length=297, Percent_Identity=31.986531986532, Blast_Score=167, Evalue=6e-43,
Organism=Escherichia coli, GI1789440, Length=286, Percent_Identity=33.5664335664336, Blast_Score=166, Evalue=1e-42,
Organism=Escherichia coli, GI145693193, Length=297, Percent_Identity=31.6498316498317, Blast_Score=164, Evalue=6e-42,
Organism=Escherichia coli, GI87081978, Length=296, Percent_Identity=31.0810810810811, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1787128, Length=287, Percent_Identity=28.5714285714286, Blast_Score=135, Evalue=2e-33,
Organism=Escherichia coli, GI1787589, Length=294, Percent_Identity=28.5714285714286, Blast_Score=121, Evalue=6e-29,
Organism=Escherichia coli, GI1789173, Length=253, Percent_Identity=30.4347826086957, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1786448, Length=290, Percent_Identity=26.2068965517241, Blast_Score=88, Evalue=6e-19,
Organism=Escherichia coli, GI1788706, Length=259, Percent_Identity=25.4826254826255, Blast_Score=88, Evalue=7e-19,
Organism=Escherichia coli, GI1790011, Length=295, Percent_Identity=26.7796610169492, Blast_Score=81, Evalue=7e-17,
Organism=Escherichia coli, GI1790262, Length=260, Percent_Identity=25.3846153846154, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI145693105, Length=193, Percent_Identity=24.8704663212435, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI157672245, Length=116, Percent_Identity=31.8965517241379, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1787601, Length=124, Percent_Identity=28.2258064516129, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1790783, Length=287, Percent_Identity=24.0418118466899, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33467; Mature: 33467

Theoretical pI: Translated: 7.73; Mature: 7.73

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDQIGAMKAFVRVVQTGSFSAAAREQSSTQATISKKVAALESLLGAKLLTRSSRELSLTE
CCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
VGAVYFENCVAIIAELDEAQAQVRSQQASPKGILRVAAPVVFGCQFIAPILGEFLIRYPE
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
IKVDLMLSDKHVDLVAEGVDLAIRAKQLEDSSLVARHLFDNPLITVASPEYLKRFTQPKE
EEEEEEECCCCHHHHHCCCCCEEEHHHCCCHHHHHHHHCCCCEEEECCHHHHHHHCCCCC
PQELRAHNCIVYSMLKSVNIWHFEQAKGGGDKISVPVGGNCRCDNGEAILQLALDGVGIA
HHHHHHHHHHHHHHHHHCCCEEECCCCCCCCEEEECCCCCEEECCCHHEEHHHHCCCCHH
QLPTWMVDKHINQGKLQRMMPNYLSHPLPFNAIYPQSRYVPLKVRCFIDYLKQCLAEEPC
HCCHHHHHHCCCHHHHHHHHHHHHCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHCCCC
YY
CC
>Mature Secondary Structure
MDQIGAMKAFVRVVQTGSFSAAAREQSSTQATISKKVAALESLLGAKLLTRSSRELSLTE
CCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
VGAVYFENCVAIIAELDEAQAQVRSQQASPKGILRVAAPVVFGCQFIAPILGEFLIRYPE
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
IKVDLMLSDKHVDLVAEGVDLAIRAKQLEDSSLVARHLFDNPLITVASPEYLKRFTQPKE
EEEEEEECCCCHHHHHCCCCCEEEHHHCCCHHHHHHHHCCCCEEEECCHHHHHHHCCCCC
PQELRAHNCIVYSMLKSVNIWHFEQAKGGGDKISVPVGGNCRCDNGEAILQLALDGVGIA
HHHHHHHHHHHHHHHHHCCCEEECCCCCCCCEEEECCCCCEEECCCHHEEHHHHCCCCHH
QLPTWMVDKHINQGKLQRMMPNYLSHPLPFNAIYPQSRYVPLKVRCFIDYLKQCLAEEPC
HCCHHHHHHCCCHHHHHHHHHHHHCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHCCCC
YY
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]