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The map label for this gene is ywbI [H]

Identifier: 157960722

GI number: 157960722

Start: 1090859

End: 1091755

Strand: Direct

Name: ywbI [H]

Synonym: Spea_0894

Alternate gene names: 157960722

Gene position: 1090859-1091755 (Clockwise)

Preceding gene: 157960718

Following gene: 157960726

Centisome position: 21.08

GC content: 49.94

Gene sequence:

>897_bases
ATGGATCTGAAGACGCTGCACTATTTTACTCAAGTGGTCGATGCCGGCGGTTTTGCTAAGGCCAGTCGCAATGTATTTCT
AACTCAGCCAGCGCTTTCTAAGGCGATTAAAGCCTTAGAGGAAGAGCTAGGTATGGTGCTGCTGGAGCGGGGAAAGCGAG
GCACTCAAATTAGGCTTACTGCAGCAGGGGAGCTGGTGTATCGCCATGCACAGCAGTTGATGACTCAGCGACAATCCATG
CTATCTGAACTTGAGGCGCTAAAAAACCTCACTGGCGGCACATTAAAGCTGGGTTTGGCGCCATTAGGCAGCGCCGAGTT
ATTTGCCCCTGTTATCGCTAAATTTAGGCAGCAATATCCTAAAGTGGATATGCAGCTGTTAGTGCGGGGCGGGGTTGAGC
AGACCCTAGCGCTACAGAAAAATGAGATTGAATTAGCGACAGGGATCATCGCATTTGACGGCGAGTTCGATGGGCTTAGG
ATCCGCAATGATCCTATGGTTGTGGTCGTACCTAAAACTCATAGGCTAGCTGAACATAAGGAGTTGCAGCTTAAGGACTT
ACAATCTCTGGCGCAGGTGATGTTCGAGCCTGAATATGCCCTCTATGAGCTGATTTTAACTGCTTGTGATAAAGCTGGTT
TTGTTCCCGCAGAAATTACCCGAGTAAGCCATGCTGATTTTGGTATCGCGCTGGTTGCTGCAGGCACAGGGATCATGGTG
TTGCCGCAGATTATCGCCGAACGTTATCGCGTCGATGCGGTGGTGAACGTGCCCCTTGCGGCGAGTGATTTAAGATGGGA
GTTGTCGCTATTTTGGCGTAAGCAGCAAAGCCTCTCTTTTGCGGCCAAGGCGATGATAGAGTTAGTTAAACAGCAACTAG
AGCAGGCGAGGCACTAA

Upstream 100 bases:

>100_bases
CCAATCTAGCGTCATTGCTGAATGACAGTCATTTTACGCTTGCCTAATTGATAAAAAAAATGAATATTAAGAATGGAATC
TATAAGTAATGGTTATCTAA

Downstream 100 bases:

>100_bases
AATAATTTGCACCCGACTCTGAATGAGTCTTTGCTCAGGTAAAATGCTATGTGTAGCCAGATCCTAGGCAAGGCAGATAA
AAATACAAAAGCCTAGTTCT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 298; Mature: 298

Protein sequence:

>298_residues
MDLKTLHYFTQVVDAGGFAKASRNVFLTQPALSKAIKALEEELGMVLLERGKRGTQIRLTAAGELVYRHAQQLMTQRQSM
LSELEALKNLTGGTLKLGLAPLGSAELFAPVIAKFRQQYPKVDMQLLVRGGVEQTLALQKNEIELATGIIAFDGEFDGLR
IRNDPMVVVVPKTHRLAEHKELQLKDLQSLAQVMFEPEYALYELILTACDKAGFVPAEITRVSHADFGIALVAAGTGIMV
LPQIIAERYRVDAVVNVPLAASDLRWELSLFWRKQQSLSFAAKAMIELVKQQLEQARH

Sequences:

>Translated_298_residues
MDLKTLHYFTQVVDAGGFAKASRNVFLTQPALSKAIKALEEELGMVLLERGKRGTQIRLTAAGELVYRHAQQLMTQRQSM
LSELEALKNLTGGTLKLGLAPLGSAELFAPVIAKFRQQYPKVDMQLLVRGGVEQTLALQKNEIELATGIIAFDGEFDGLR
IRNDPMVVVVPKTHRLAEHKELQLKDLQSLAQVMFEPEYALYELILTACDKAGFVPAEITRVSHADFGIALVAAGTGIMV
LPQIIAERYRVDAVVNVPLAASDLRWELSLFWRKQQSLSFAAKAMIELVKQQLEQARH
>Mature_298_residues
MDLKTLHYFTQVVDAGGFAKASRNVFLTQPALSKAIKALEEELGMVLLERGKRGTQIRLTAAGELVYRHAQQLMTQRQSM
LSELEALKNLTGGTLKLGLAPLGSAELFAPVIAKFRQQYPKVDMQLLVRGGVEQTLALQKNEIELATGIIAFDGEFDGLR
IRNDPMVVVVPKTHRLAEHKELQLKDLQSLAQVMFEPEYALYELILTACDKAGFVPAEITRVSHADFGIALVAAGTGIMV
LPQIIAERYRVDAVVNVPLAASDLRWELSLFWRKQQSLSFAAKAMIELVKQQLEQARH

Specific function: Positive Regulatory Protein For The Cyn Operon. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI157672245, Length=287, Percent_Identity=27.1777003484321, Blast_Score=110, Evalue=8e-26,
Organism=Escherichia coli, GI1787879, Length=291, Percent_Identity=28.5223367697594, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI2367136, Length=250, Percent_Identity=27.2, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1790399, Length=306, Percent_Identity=27.4509803921569, Blast_Score=95, Evalue=6e-21,
Organism=Escherichia coli, GI1788887, Length=302, Percent_Identity=27.4834437086093, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI145693105, Length=289, Percent_Identity=23.5294117647059, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1789639, Length=281, Percent_Identity=25.6227758007117, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1788748, Length=293, Percent_Identity=25.5972696245734, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1789506, Length=293, Percent_Identity=20.8191126279863, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1788297, Length=147, Percent_Identity=27.891156462585, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787601, Length=149, Percent_Identity=29.5302013422819, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1786401, Length=122, Percent_Identity=32.7868852459016, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI87081904, Length=146, Percent_Identity=29.4520547945205, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1787530, Length=291, Percent_Identity=24.0549828178694, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1790262, Length=257, Percent_Identity=23.3463035019455, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1789440, Length=123, Percent_Identity=27.6422764227642, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33091; Mature: 33091

Theoretical pI: Translated: 8.43; Mature: 8.43

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLKTLHYFTQVVDAGGFAKASRNVFLTQPALSKAIKALEEELGMVLLERGKRGTQIRLT
CCCHHHHHHHHHHHCCCCHHCCCCEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEE
AAGELVYRHAQQLMTQRQSMLSELEALKNLTGGTLKLGLAPLGSAELFAPVIAKFRQQYP
EHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHCC
KVDMQLLVRGGVEQTLALQKNEIELATGIIAFDGEFDGLRIRNDPMVVVVPKTHRLAEHK
CCCHHHHHHCCHHHHHHHCCCCEEEEEEEEEECCCCCCEEEECCCEEEEECCCHHHHHHH
ELQLKDLQSLAQVMFEPEYALYELILTACDKAGFVPAEITRVSHADFGIALVAAGTGIMV
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCEEEEECCCCHHH
LPQIIAERYRVDAVVNVPLAASDLRWELSLFWRKQQSLSFAAKAMIELVKQQLEQARH
HHHHHHHHHHHCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDLKTLHYFTQVVDAGGFAKASRNVFLTQPALSKAIKALEEELGMVLLERGKRGTQIRLT
CCCHHHHHHHHHHHCCCCHHCCCCEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEE
AAGELVYRHAQQLMTQRQSMLSELEALKNLTGGTLKLGLAPLGSAELFAPVIAKFRQQYP
EHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHCC
KVDMQLLVRGGVEQTLALQKNEIELATGIIAFDGEFDGLRIRNDPMVVVVPKTHRLAEHK
CCCHHHHHHCCHHHHHHHCCCCEEEEEEEEEECCCCCCEEEECCCEEEEECCCHHHHHHH
ELQLKDLQSLAQVMFEPEYALYELILTACDKAGFVPAEITRVSHADFGIALVAAGTGIMV
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCEEEEECCCCHHH
LPQIIAERYRVDAVVNVPLAASDLRWELSLFWRKQQSLSFAAKAMIELVKQQLEQARH
HHHHHHHHHHHCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]