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The map label for this gene is rsuA [H]

Identifier: 157960717

GI number: 157960717

Start: 1085524

End: 1086228

Strand: Direct

Name: rsuA [H]

Synonym: Spea_0889

Alternate gene names: 157960717

Gene position: 1085524-1086228 (Clockwise)

Preceding gene: 157960716

Following gene: 157960718

Centisome position: 20.98

GC content: 47.09

Gene sequence:

>705_bases
ATGGAATCAAAACGTGCGCGCTTAGATAGGTTTATCAGTGCAAAAACCGGCATCAATAAAAAACATGTCAGATTGCTACT
CGCCAAAGGCCAAGTGTTGGTGGATGGTGAAGTGGCCCGAGATATAGACCAGATCATCGATGAGTTCTCCCATGTTTGCT
TAGACGGCAAGGTCTTACAAGCCAATGCGCCAAGTTATGTAATGCTCTATAAACCAGTCGGTGTGGTCAGTGCCACAGTG
GACGACAAGCATAGGACGGTTATCGACCTACTTGAGCGGGACGATAAACACAGTCTGCATATAGTCGGACGATTAGATTT
GAATACCTCGGGATTACTGTTACTCACCAACGATGGTCGCTGGTCGAAGAAGTTGATGTCGCCGGATCATAAGGTGGGCA
AGGTGTATAGGGTGACATTGAAAAACCCTATCGATGAATCCTATATTGCTGCATTTGCTGGTGGAATGTACTTTGCGTTC
GAGGATATCACCACTCAACCGGCCAAACTTGAAATTGTCGATGAACATACGGCCTTGGTGACCTTAGTCGAAGGGCGCTA
TCACCAAATTAAGCGGATGTTTGGTCGTTTTCGAAACCCGGTTATAGGCCTACACAGACTATCGGTTGGTGCTATCGAGC
TGGATCCACAATTGGCTGTGGGGCAGAGCCGAGACTTATCGATGACAGAGATCCAAATAGCCTAA

Upstream 100 bases:

>100_bases
AGAGGACGCCACGGTAAGTCGTGAAGAGCATATTATCGAAGGTGCGCGGCGTTGCGCCTATAAAGTGCTGCCTAGCAATA
AAGCGAGCGGTCAATAAGTC

Downstream 100 bases:

>100_bases
CGAGTGGCGCTAGCCTTAGCTGAACGCAGTGACTGAATAGCGAGTTGTAACTGAGCTATGTATGAGGAATTTCGTTTCTC
AAAACGACGTTTTACAGTGT

Product: pseudouridine synthase

Products: pseudouridine 5'-phosphate; H2O [C]

Alternate protein names: 16S pseudouridine 516 synthase; 16S pseudouridylate 516 synthase; rRNA pseudouridylate synthase A; rRNA-uridine isomerase A [H]

Number of amino acids: Translated: 234; Mature: 234

Protein sequence:

>234_residues
MESKRARLDRFISAKTGINKKHVRLLLAKGQVLVDGEVARDIDQIIDEFSHVCLDGKVLQANAPSYVMLYKPVGVVSATV
DDKHRTVIDLLERDDKHSLHIVGRLDLNTSGLLLLTNDGRWSKKLMSPDHKVGKVYRVTLKNPIDESYIAAFAGGMYFAF
EDITTQPAKLEIVDEHTALVTLVEGRYHQIKRMFGRFRNPVIGLHRLSVGAIELDPQLAVGQSRDLSMTEIQIA

Sequences:

>Translated_234_residues
MESKRARLDRFISAKTGINKKHVRLLLAKGQVLVDGEVARDIDQIIDEFSHVCLDGKVLQANAPSYVMLYKPVGVVSATV
DDKHRTVIDLLERDDKHSLHIVGRLDLNTSGLLLLTNDGRWSKKLMSPDHKVGKVYRVTLKNPIDESYIAAFAGGMYFAF
EDITTQPAKLEIVDEHTALVTLVEGRYHQIKRMFGRFRNPVIGLHRLSVGAIELDPQLAVGQSRDLSMTEIQIA
>Mature_234_residues
MESKRARLDRFISAKTGINKKHVRLLLAKGQVLVDGEVARDIDQIIDEFSHVCLDGKVLQANAPSYVMLYKPVGVVSATV
DDKHRTVIDLLERDDKHSLHIVGRLDLNTSGLLLLTNDGRWSKKLMSPDHKVGKVYRVTLKNPIDESYIAAFAGGMYFAF
EDITTQPAKLEIVDEHTALVTLVEGRYHQIKRMFGRFRNPVIGLHRLSVGAIELDPQLAVGQSRDLSMTEIQIA

Specific function: Responsible for synthesis of pseudouridine from uracil- 516 in 16S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788510, Length=226, Percent_Identity=40.2654867256637, Blast_Score=166, Evalue=2e-42,
Organism=Escherichia coli, GI1790453, Length=230, Percent_Identity=27.8260869565217, Blast_Score=77, Evalue=8e-16,
Organism=Escherichia coli, GI87081838, Length=186, Percent_Identity=30.6451612903226, Blast_Score=74, Evalue=8e-15,
Organism=Escherichia coli, GI1787524, Length=224, Percent_Identity=27.2321428571429, Blast_Score=70, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: 4.2.1.70 [C]

Molecular weight: Translated: 26226; Mature: 26226

Theoretical pI: Translated: 8.94; Mature: 8.94

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESKRARLDRFISAKTGINKKHVRLLLAKGQVLVDGEVARDIDQIIDEFSHVCLDGKVLQ
CCCHHHHHHHHHHHHCCCCHHHEEEEEECCCEEECCHHHHHHHHHHHHHHHHHCCCEEEE
ANAPSYVMLYKPVGVVSATVDDKHRTVIDLLERDDKHSLHIVGRLDLNTSGLLLLTNDGR
CCCCCEEEEEECCCEEEEECCCCHHHHHHHHCCCCCCEEEEEEEEECCCCCEEEEECCCC
WSKKLMSPDHKVGKVYRVTLKNPIDESYIAAFAGGMYFAFEDITTQPAKLEIVDEHTALV
HHHHHCCCCCCCCEEEEEEECCCCCHHHHHHHHCCEEEEEECCCCCCEEEEEEECCEEEE
TLVEGRYHQIKRMFGRFRNPVIGLHRLSVGAIELDPQLAVGQSRDLSMTEIQIA
EEECCHHHHHHHHHHHHCCCEEEEEEEEEEEEEECCCEECCCCCCCEEEEEEEC
>Mature Secondary Structure
MESKRARLDRFISAKTGINKKHVRLLLAKGQVLVDGEVARDIDQIIDEFSHVCLDGKVLQ
CCCHHHHHHHHHHHHCCCCHHHEEEEEECCCEEECCHHHHHHHHHHHHHHHHHCCCEEEE
ANAPSYVMLYKPVGVVSATVDDKHRTVIDLLERDDKHSLHIVGRLDLNTSGLLLLTNDGR
CCCCCEEEEEECCCEEEEECCCCHHHHHHHHCCCCCCEEEEEEEEECCCCCEEEEECCCC
WSKKLMSPDHKVGKVYRVTLKNPIDESYIAAFAGGMYFAFEDITTQPAKLEIVDEHTALV
HHHHHCCCCCCCCEEEEEEECCCCCHHHHHHHHCCEEEEEECCCCCCEEEEEEECCEEEE
TLVEGRYHQIKRMFGRFRNPVIGLHRLSVGAIELDPQLAVGQSRDLSMTEIQIA
EEECCHHHHHHHHHHHHCCCEEEEEEEEEEEEEECCCEECCCCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose phosphate [C]

Specific reaction: uracil + D-ribose phosphate = pseudouridine 5'-phosphate + H2O [C]

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]