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The map label for this gene is ispB [H]

Identifier: 157960679

GI number: 157960679

Start: 1050985

End: 1051956

Strand: Reverse

Name: ispB [H]

Synonym: Spea_0851

Alternate gene names: 157960679

Gene position: 1051956-1050985 (Counterclockwise)

Preceding gene: 157960687

Following gene: 157960677

Centisome position: 20.33

GC content: 44.65

Gene sequence:

>972_bases
ATGGATTTAAACGCCATTCGTCAATTGACTGATAGCGATATGCAAGCTGTCAATCAACTGATCTATAAACAACTAGAGTC
AGATGTTGCCCTAATTAATCAACTTGGGTTCTACATTATTAACGGTGGCGGTAAACGCTTGCGACCTCTGCTGTCTATTC
TAGCAGCTCGTGCCATTGACTATAAAGGTGATTCTCACCTTAAATTGGCCGCCATTATCGAATTCATTCACACTGCGTCA
CTGTTACACGATGATGTCGTTGATGAGTCGATGTTGCGCCGTGGTAGAAAAACCGCCAATGCCTTATTCGGAAATAGCGC
AAGTGTATTAGTCGGTGACTTCCTATACACACGCTCATTCCAGATGATGACAGAGCTTCGTAGCTTAGACGTGCTAGAGA
TCCTTGCAGACGCAACCAACGTACTAGCAGAGGGTGAAGTCCTACAGTTAATGAACTGTAACGACCCTGACACCACTGAA
GATAGCTATATGCGTGTTATCTATTGCAAGACAGCAAAACTGTTTGAGGCGGCTACTCGCCTTGCAGGATTACTCGCTGA
CAGCCCAATAGAAGTGCAAACGGCGCTAGCTGACTACGGTAAGTATTTGGGCACTGCATTCCAGTTAACCGATGACCTAC
TCGATTACACCGCAGACACAGAAGAGCTTGGCAAAAACATCGGTGATGACTTAGCCGAAGGTAAGCCAACATTGCCGCTT
ATTTTTGCAATGGCAAACGGTAATGACACTGAAAAAGCCCTTATCCGTGATGCTATCGAGAAAGGTGACGGTACAGAACA
TATCGACGTCATTCTCGCCGCACTTAAAAACTGTGGCGCGTTAGAGTATACAGAGCAGCGTGCAATTGAAGAATCAGAAA
AAGCAATTGCGGCCTTATCAATTATTCCTGACTCTGATTATAAAAAAGCGCTCATCTCTTTAGCTAAAATTGCGACTCAA
CGTAGCAATTAG

Upstream 100 bases:

>100_bases
TCTTGTACCGTAAATTTAGGTAGAATCCAATTATTATAACATTTAAGCCTTAATTGAGCTGCAATGTTACTCAGCAGCCA
ACAAGAACTAGAGCCTACTT

Downstream 100 bases:

>100_bases
TCAACAAGGCTAAATCAGAAACCAAAAAAGGGAGCCTAGTGGCTCCCTTTTTAATTGCAGCTAATTGATTACTTGACGAA
ATCAATACCTAGTTGGATAT

Product: polyprenyl synthetase

Products: NA

Alternate protein names: All-trans-octaprenyl-diphosphate synthase; Octaprenyl pyrophosphate synthase; OPP synthase [H]

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MDLNAIRQLTDSDMQAVNQLIYKQLESDVALINQLGFYIINGGGKRLRPLLSILAARAIDYKGDSHLKLAAIIEFIHTAS
LLHDDVVDESMLRRGRKTANALFGNSASVLVGDFLYTRSFQMMTELRSLDVLEILADATNVLAEGEVLQLMNCNDPDTTE
DSYMRVIYCKTAKLFEAATRLAGLLADSPIEVQTALADYGKYLGTAFQLTDDLLDYTADTEELGKNIGDDLAEGKPTLPL
IFAMANGNDTEKALIRDAIEKGDGTEHIDVILAALKNCGALEYTEQRAIEESEKAIAALSIIPDSDYKKALISLAKIATQ
RSN

Sequences:

>Translated_323_residues
MDLNAIRQLTDSDMQAVNQLIYKQLESDVALINQLGFYIINGGGKRLRPLLSILAARAIDYKGDSHLKLAAIIEFIHTAS
LLHDDVVDESMLRRGRKTANALFGNSASVLVGDFLYTRSFQMMTELRSLDVLEILADATNVLAEGEVLQLMNCNDPDTTE
DSYMRVIYCKTAKLFEAATRLAGLLADSPIEVQTALADYGKYLGTAFQLTDDLLDYTADTEELGKNIGDDLAEGKPTLPL
IFAMANGNDTEKALIRDAIEKGDGTEHIDVILAALKNCGALEYTEQRAIEESEKAIAALSIIPDSDYKKALISLAKIATQ
RSN
>Mature_323_residues
MDLNAIRQLTDSDMQAVNQLIYKQLESDVALINQLGFYIINGGGKRLRPLLSILAARAIDYKGDSHLKLAAIIEFIHTAS
LLHDDVVDESMLRRGRKTANALFGNSASVLVGDFLYTRSFQMMTELRSLDVLEILADATNVLAEGEVLQLMNCNDPDTTE
DSYMRVIYCKTAKLFEAATRLAGLLADSPIEVQTALADYGKYLGTAFQLTDDLLDYTADTEELGKNIGDDLAEGKPTLPL
IFAMANGNDTEKALIRDAIEKGDGTEHIDVILAALKNCGALEYTEQRAIEESEKAIAALSIIPDSDYKKALISLAKIATQ
RSN

Specific function: Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=321, Percent_Identity=29.9065420560748, Blast_Score=137, Evalue=9e-33,
Organism=Homo sapiens, GI4758430, Length=262, Percent_Identity=26.7175572519084, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI83700220, Length=262, Percent_Identity=26.7175572519084, Blast_Score=81, Evalue=1e-15,
Organism=Escherichia coli, GI1789578, Length=321, Percent_Identity=62.9283489096573, Blast_Score=433, Evalue=1e-123,
Organism=Escherichia coli, GI1786623, Length=229, Percent_Identity=33.6244541484716, Blast_Score=93, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17505681, Length=321, Percent_Identity=31.4641744548287, Blast_Score=153, Evalue=1e-37,
Organism=Saccharomyces cerevisiae, GI6319475, Length=289, Percent_Identity=33.9100346020761, Blast_Score=138, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6325188, Length=227, Percent_Identity=26.8722466960352, Blast_Score=83, Evalue=7e-17,
Organism=Drosophila melanogaster, GI24651612, Length=292, Percent_Identity=31.8493150684932, Blast_Score=149, Evalue=2e-36,
Organism=Drosophila melanogaster, GI281365769, Length=274, Percent_Identity=24.8175182481752, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24660002, Length=274, Percent_Identity=24.8175182481752, Blast_Score=75, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.90 [H]

Molecular weight: Translated: 35372; Mature: 35372

Theoretical pI: Translated: 4.36; Mature: 4.36

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1 ; PS00444 POLYPRENYL_SYNTHET_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLNAIRQLTDSDMQAVNQLIYKQLESDVALINQLGFYIINGGGKRLRPLLSILAARAID
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHHC
YKGDSHLKLAAIIEFIHTASLLHDDVVDESMLRRGRKTANALFGNSASVLVGDFLYTRSF
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHHHHHH
QMMTELRSLDVLEILADATNVLAEGEVLQLMNCNDPDTTEDSYMRVIYCKTAKLFEAATR
HHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCEEEEEEEHHHHHHHHHHH
LAGLLADSPIEVQTALADYGKYLGTAFQLTDDLLDYTADTEELGKNIGDDLAEGKPTLPL
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCHHHHCCCCCCEE
IFAMANGNDTEKALIRDAIEKGDGTEHIDVILAALKNCGALEYTEQRAIEESEKAIAALS
EEEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHEE
IIPDSDYKKALISLAKIATQRSN
ECCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDLNAIRQLTDSDMQAVNQLIYKQLESDVALINQLGFYIINGGGKRLRPLLSILAARAID
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHHC
YKGDSHLKLAAIIEFIHTASLLHDDVVDESMLRRGRKTANALFGNSASVLVGDFLYTRSF
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHHHHHH
QMMTELRSLDVLEILADATNVLAEGEVLQLMNCNDPDTTEDSYMRVIYCKTAKLFEAATR
HHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCEEEEEEEHHHHHHHHHHH
LAGLLADSPIEVQTALADYGKYLGTAFQLTDDLLDYTADTEELGKNIGDDLAEGKPTLPL
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCHHHHCCCCCCEE
IFAMANGNDTEKALIRDAIEKGDGTEHIDVILAALKNCGALEYTEQRAIEESEKAIAALS
EEEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHEE
IIPDSDYKKALISLAKIATQRSN
ECCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8312607; 9278503; 2670911; 8037730 [H]