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The map label for this gene is ligT [C]

Identifier: 157960531

GI number: 157960531

Start: 863577

End: 864098

Strand: Reverse

Name: ligT [C]

Synonym: Spea_0703

Alternate gene names: 157960531

Gene position: 864098-863577 (Counterclockwise)

Preceding gene: 157960535

Following gene: 157960530

Centisome position: 16.7

GC content: 48.47

Gene sequence:

>522_bases
ATGCCGACTCAAACCAAGCGCGTGTTCTTAGGTTTTTCACTCAGTCAAACCCAGACTCAAGCGATAACCGCAATCCAGGT
GCAGCTGCCTAATAATGTCCGCTTAGTGCCCAGCGCCAACTTGCATATGACTCTAGCCTTCTTCGGCGCAGCAACACCAG
CACAATTATCGGCGCTTATCGATAAGGTTAATCGTCTGCATAAACCTAAATTTAGCGTCACTCTCGATACGTTAAGCCAC
TGGGCTAAACCTAAGATCCTCTGTCTCAGAGGGGATGCCAGTGATAATCGCCTACTCCAGCTCGCTCTCGATACTCAAAG
CCTTGCAAACGAACTTGGCTTACATTGCAGTGAACATCACTATAATCCCCACATCACCCTATCGCGCAAGGCAAAGGCTG
AAGTGAGCGGTGTCGATTATCAAGCCATAATTCTACACCCCAAACGGTTACAGCTGTTTGAATCCCATCCAGGCCCTAAT
GGGGTCGAATATCCAACACTCCACAGTTGGCCATTGTCTTAA

Upstream 100 bases:

>100_bases
AGCCTAGACTAAACTGCTTGGTTCAGCATTGAGCTTAATATCATGGCAGTTTAGCTCGTATTTTATCTAAATCCGTTAAT
TCAAACACTGGGAGCCTAAT

Downstream 100 bases:

>100_bases
GGATTAACCTTAACCCCATCAAAGCTGCACCGGTTATAGTCTTATCCCGGCCACTTACATTAGGGGAGAATATCCCCAAC
ACTTCACTCATGTCACTCTT

Product: 2'-5' RNA ligase

Products: NA

Alternate protein names: 2 5' RNA Ligase; 2\-5\' RNA Ligase; 2-5 RNA Ligase; 2 5 RNA Ligase; Phosphoesterase HXTX

Number of amino acids: Translated: 173; Mature: 172

Protein sequence:

>173_residues
MPTQTKRVFLGFSLSQTQTQAITAIQVQLPNNVRLVPSANLHMTLAFFGAATPAQLSALIDKVNRLHKPKFSVTLDTLSH
WAKPKILCLRGDASDNRLLQLALDTQSLANELGLHCSEHHYNPHITLSRKAKAEVSGVDYQAIILHPKRLQLFESHPGPN
GVEYPTLHSWPLS

Sequences:

>Translated_173_residues
MPTQTKRVFLGFSLSQTQTQAITAIQVQLPNNVRLVPSANLHMTLAFFGAATPAQLSALIDKVNRLHKPKFSVTLDTLSH
WAKPKILCLRGDASDNRLLQLALDTQSLANELGLHCSEHHYNPHITLSRKAKAEVSGVDYQAIILHPKRLQLFESHPGPN
GVEYPTLHSWPLS
>Mature_172_residues
PTQTKRVFLGFSLSQTQTQAITAIQVQLPNNVRLVPSANLHMTLAFFGAATPAQLSALIDKVNRLHKPKFSVTLDTLSHW
AKPKILCLRGDASDNRLLQLALDTQSLANELGLHCSEHHYNPHITLSRKAKAEVSGVDYQAIILHPKRLQLFESHPGPNG
VEYPTLHSWPLS

Specific function: Specifically Ligates tRNA Half-Molecules Containing Nucleoside Base Modifications And Shows A Preference Among Different tRNA Species. Acts In The Absence Of ATP To Form A 2'-5' Phosphodiester Linkage. [C]

COG id: COG1514

COG function: function code J; 2'-5' RNA ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87081692, Length=177, Percent_Identity=27.683615819209, Blast_Score=64, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 6.5.1.- [C]

Molecular weight: Translated: 19198; Mature: 19067

Theoretical pI: Translated: 9.89; Mature: 9.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTQTKRVFLGFSLSQTQTQAITAIQVQLPNNVRLVPSANLHMTLAFFGAATPAQLSALI
CCCCCEEEEEEEECCCCCCEEEEEEEEECCCCEEEEECCCEEEEEEEECCCCHHHHHHHH
DKVNRLHKPKFSVTLDTLSHWAKPKILCLRGDASDNRLLQLALDTQSLANELGLHCSEHH
HHHHHHCCCCEEEEHHHHHHCCCCEEEEEECCCCCCEEEEEEECHHHHHHHHCCCCCCCC
YNPHITLSRKAKAEVSGVDYQAIILHPKRLQLFESHPGPNGVEYPTLHSWPLS
CCCCEEEECCCCCEECCCCEEEEEECCHHHHHHHCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PTQTKRVFLGFSLSQTQTQAITAIQVQLPNNVRLVPSANLHMTLAFFGAATPAQLSALI
CCCCEEEEEEEECCCCCCEEEEEEEEECCCCEEEEECCCEEEEEEEECCCCHHHHHHHH
DKVNRLHKPKFSVTLDTLSHWAKPKILCLRGDASDNRLLQLALDTQSLANELGLHCSEHH
HHHHHHCCCCEEEEHHHHHHCCCCEEEEEECCCCCCEEEEEEECHHHHHHHHCCCCCCCC
YNPHITLSRKAKAEVSGVDYQAIILHPKRLQLFESHPGPNGVEYPTLHSWPLS
CCCCEEEECCCCCEECCCCEEEEEECCHHHHHHHCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA